Molecular evolution of influenza A/H3N2 viruses in the province of Québec (Canada) during the 1997-2000 period.
Identifieur interne : 000A77 ( Main/Curation ); précédent : 000A76; suivant : 000A78Molecular evolution of influenza A/H3N2 viruses in the province of Québec (Canada) during the 1997-2000 period.
Auteurs : I. Hardy [Canada] ; Y. Li ; M B Coulthart ; N. Goyette ; G. BoivinSource :
- Virus research [ 0168-1702 ] ; 2001.
Descripteurs français
- KwdFr :
- ADN viral (génétique), Analyse de séquence d'ADN (MeSH), Antigènes viraux (immunologie), Données de séquences moléculaires (MeSH), Glycoprotéine hémagglutinine du virus influenza (génétique), Grippe humaine (virologie), Grippe humaine (épidémiologie), Gènes viraux (MeSH), Humains (MeSH), Phylogenèse (MeSH), Québec (épidémiologie), Sous-type H3N2 du virus de la grippe A (MeSH), Tests d'inhibition de l'hémagglutination (MeSH), Virus de la grippe A (génétique), Virus de la grippe A (immunologie), Évolution moléculaire (MeSH).
- MESH :
- génétique : ADN viral, Glycoprotéine hémagglutinine du virus influenza, Virus de la grippe A.
- immunologie : Antigènes viraux, Virus de la grippe A.
- virologie : Grippe humaine.
- épidémiologie : Grippe humaine, Québec.
- Analyse de séquence d'ADN, Données de séquences moléculaires, Gènes viraux, Humains, Phylogenèse, Sous-type H3N2 du virus de la grippe A, Tests d'inhibition de l'hémagglutination, Évolution moléculaire.
English descriptors
- KwdEn :
- Antigens, Viral (immunology), DNA, Viral (genetics), Evolution, Molecular (MeSH), Genes, Viral (MeSH), Hemagglutination Inhibition Tests (MeSH), Hemagglutinin Glycoproteins, Influenza Virus (genetics), Humans (MeSH), Influenza A Virus, H3N2 Subtype (MeSH), Influenza A virus (genetics), Influenza A virus (immunology), Influenza, Human (epidemiology), Influenza, Human (virology), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Quebec (epidemiology), Sequence Analysis, DNA (MeSH).
- MESH :
- chemical , genetics : DNA, Viral, Hemagglutinin Glycoproteins, Influenza Virus.
- chemical , immunology : Antigens, Viral.
- geographic , epidemiology : Quebec.
- epidemiology : Influenza, Human.
- genetics : Influenza A virus.
- immunology : Influenza A virus.
- virology : Influenza, Human.
- Evolution, Molecular, Genes, Viral, Hemagglutination Inhibition Tests, Humans, Influenza A Virus, H3N2 Subtype, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA.
Abstract
In this study, we compared antigenic (hemagglutination inhibition (HI) assay) and molecular (sequencing of the hemagglutinin (HA1) gene) characterization of influenza isolates collected in the Province of Québec (Canada) during the last three flu seasons (1997-2000). Twenty-three isolates were tested by a standard HI assay and 37 by sequencing of the HA1 gene for their homology to the A/H3N2 vaccine strains A/Wuhan/359/95 (1997-1998) and A/Sydney/5/97 (1998-1999 and 1999-2000). By HI, two isolates were antigenically similar to A/Wuhan/359/95 (both from 1997 to 1998), 16 were similar to A/Sydney/5/97 (1997-2000) and no conclusions could be inferred for the other five isolates due to identical HI titers for the two vaccine strains (n=4) or insufficient viral titer (n=1). Sequence analysis revealed that four isolates from 1997 to 1998 were related to A/Wuhan/359/95 whereas the others (n=4) from 1997 to 1998, as well as all isolates from 1998 to 1999 (n=18) and 1999 to 2000 (n=11) were closer to A/Sydney/5/97. The mean number of amino acid differences for the 33 A/Sydney/5/97-like isolates compared with the homologous vaccine strain was 6.3 (1.9%), 9.2 (2.8%) and 13.6 (4.1%) for those collected in 1997-1998, 1998-1999, and 1999-2000, respectively. Phylogenetic analysis confirmed that a progressive drift occurred among our A/H3N2 influenza isolates over the last three flu seasons. Furthermore, it revealed that isolates collected during the last two flu seasons were in fact more related to A/Panama/2007/99 (2000-2001 vaccine strain) than to A/Sydney/5/97. Our studies suggest that molecular analysis of the HA1 gene should complement the HI assay for a more accurate analysis of influenza A virus drift.
DOI: 10.1016/s0168-1702(01)00269-6
PubMed: 11451491
Links toward previous steps (curation, corpus...)
- to stream Main, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000A77
Links to Exploration step
pubmed:11451491Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular evolution of influenza A/H3N2 viruses in the province of Québec (Canada) during the 1997-2000 period.</title><author><name sortKey="Hardy, I" sort="Hardy, I" uniqKey="Hardy I" first="I" last="Hardy">I. Hardy</name><affiliation wicri:level="1"><nlm:affiliation>Infectious Disease Research Center, Centre Hospitalier Universitaire de Québec and Laval University, Pavillon, CHUL RC-709, 2705 blvd Laurier, Québec, GIV 4G2, Sainte-Foy, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Infectious Disease Research Center, Centre Hospitalier Universitaire de Québec and Laval University, Pavillon, CHUL RC-709, 2705 blvd Laurier, Québec, GIV 4G2, Sainte-Foy</wicri:regionArea></affiliation></author><author><name sortKey="Li, Y" sort="Li, Y" uniqKey="Li Y" first="Y" last="Li">Y. Li</name></author><author><name sortKey="Coulthart, M B" sort="Coulthart, M B" uniqKey="Coulthart M" first="M B" last="Coulthart">M B Coulthart</name></author><author><name sortKey="Goyette, N" sort="Goyette, N" uniqKey="Goyette N" first="N" last="Goyette">N. Goyette</name></author><author><name sortKey="Boivin, G" sort="Boivin, G" uniqKey="Boivin G" first="G" last="Boivin">G. Boivin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11451491</idno><idno type="pmid">11451491</idno><idno type="doi">10.1016/s0168-1702(01)00269-6</idno><idno type="wicri:Area/Main/Corpus">000A77</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000A77</idno><idno type="wicri:Area/Main/Curation">000A77</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000A77</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular evolution of influenza A/H3N2 viruses in the province of Québec (Canada) during the 1997-2000 period.</title><author><name sortKey="Hardy, I" sort="Hardy, I" uniqKey="Hardy I" first="I" last="Hardy">I. Hardy</name><affiliation wicri:level="1"><nlm:affiliation>Infectious Disease Research Center, Centre Hospitalier Universitaire de Québec and Laval University, Pavillon, CHUL RC-709, 2705 blvd Laurier, Québec, GIV 4G2, Sainte-Foy, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Infectious Disease Research Center, Centre Hospitalier Universitaire de Québec and Laval University, Pavillon, CHUL RC-709, 2705 blvd Laurier, Québec, GIV 4G2, Sainte-Foy</wicri:regionArea></affiliation></author><author><name sortKey="Li, Y" sort="Li, Y" uniqKey="Li Y" first="Y" last="Li">Y. Li</name></author><author><name sortKey="Coulthart, M B" sort="Coulthart, M B" uniqKey="Coulthart M" first="M B" last="Coulthart">M B Coulthart</name></author><author><name sortKey="Goyette, N" sort="Goyette, N" uniqKey="Goyette N" first="N" last="Goyette">N. Goyette</name></author><author><name sortKey="Boivin, G" sort="Boivin, G" uniqKey="Boivin G" first="G" last="Boivin">G. Boivin</name></author></analytic><series><title level="j">Virus research</title><idno type="ISSN">0168-1702</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigens, Viral (immunology)</term><term>DNA, Viral (genetics)</term><term>Evolution, Molecular (MeSH)</term><term>Genes, Viral (MeSH)</term><term>Hemagglutination Inhibition Tests (MeSH)</term><term>Hemagglutinin Glycoproteins, Influenza Virus (genetics)</term><term>Humans (MeSH)</term><term>Influenza A Virus, H3N2 Subtype (MeSH)</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (immunology)</term><term>Influenza, Human (epidemiology)</term><term>Influenza, Human (virology)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Quebec (epidemiology)</term><term>Sequence Analysis, DNA (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Antigènes viraux (immunologie)</term><term>Données de séquences moléculaires (MeSH)</term><term>Glycoprotéine hémagglutinine du virus influenza (génétique)</term><term>Grippe humaine (virologie)</term><term>Grippe humaine (épidémiologie)</term><term>Gènes viraux (MeSH)</term><term>Humains (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Québec (épidémiologie)</term><term>Sous-type H3N2 du virus de la grippe A (MeSH)</term><term>Tests d'inhibition de l'hémagglutination (MeSH)</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (immunologie)</term><term>Évolution moléculaire (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term><term>Hemagglutinin Glycoproteins, Influenza Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Quebec</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term><term>Glycoprotéine hémagglutinine du virus influenza</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes viraux</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Grippe humaine</term><term>Québec</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Evolution, Molecular</term><term>Genes, Viral</term><term>Hemagglutination Inhibition Tests</term><term>Humans</term><term>Influenza A Virus, H3N2 Subtype</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Gènes viraux</term><term>Humains</term><term>Phylogenèse</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Tests d'inhibition de l'hémagglutination</term><term>Évolution moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study, we compared antigenic (hemagglutination inhibition (HI) assay) and molecular (sequencing of the hemagglutinin (HA1) gene) characterization of influenza isolates collected in the Province of Québec (Canada) during the last three flu seasons (1997-2000). Twenty-three isolates were tested by a standard HI assay and 37 by sequencing of the HA1 gene for their homology to the A/H3N2 vaccine strains A/Wuhan/359/95 (1997-1998) and A/Sydney/5/97 (1998-1999 and 1999-2000). By HI, two isolates were antigenically similar to A/Wuhan/359/95 (both from 1997 to 1998), 16 were similar to A/Sydney/5/97 (1997-2000) and no conclusions could be inferred for the other five isolates due to identical HI titers for the two vaccine strains (n=4) or insufficient viral titer (n=1). Sequence analysis revealed that four isolates from 1997 to 1998 were related to A/Wuhan/359/95 whereas the others (n=4) from 1997 to 1998, as well as all isolates from 1998 to 1999 (n=18) and 1999 to 2000 (n=11) were closer to A/Sydney/5/97. The mean number of amino acid differences for the 33 A/Sydney/5/97-like isolates compared with the homologous vaccine strain was 6.3 (1.9%), 9.2 (2.8%) and 13.6 (4.1%) for those collected in 1997-1998, 1998-1999, and 1999-2000, respectively. Phylogenetic analysis confirmed that a progressive drift occurred among our A/H3N2 influenza isolates over the last three flu seasons. Furthermore, it revealed that isolates collected during the last two flu seasons were in fact more related to A/Panama/2007/99 (2000-2001 vaccine strain) than to A/Sydney/5/97. Our studies suggest that molecular analysis of the HA1 gene should complement the HI assay for a more accurate analysis of influenza A virus drift.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11451491</PMID><DateCompleted><Year>2001</Year><Month>10</Month><Day>25</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0168-1702</ISSN><JournalIssue CitedMedium="Print"><Volume>77</Volume><Issue>1</Issue><PubDate><Year>2001</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Virus research</Title><ISOAbbreviation>Virus Res.</ISOAbbreviation></Journal><ArticleTitle>Molecular evolution of influenza A/H3N2 viruses in the province of Québec (Canada) during the 1997-2000 period.</ArticleTitle><Pagination><MedlinePgn>89-96</MedlinePgn></Pagination><Abstract><AbstractText>In this study, we compared antigenic (hemagglutination inhibition (HI) assay) and molecular (sequencing of the hemagglutinin (HA1) gene) characterization of influenza isolates collected in the Province of Québec (Canada) during the last three flu seasons (1997-2000). Twenty-three isolates were tested by a standard HI assay and 37 by sequencing of the HA1 gene for their homology to the A/H3N2 vaccine strains A/Wuhan/359/95 (1997-1998) and A/Sydney/5/97 (1998-1999 and 1999-2000). By HI, two isolates were antigenically similar to A/Wuhan/359/95 (both from 1997 to 1998), 16 were similar to A/Sydney/5/97 (1997-2000) and no conclusions could be inferred for the other five isolates due to identical HI titers for the two vaccine strains (n=4) or insufficient viral titer (n=1). Sequence analysis revealed that four isolates from 1997 to 1998 were related to A/Wuhan/359/95 whereas the others (n=4) from 1997 to 1998, as well as all isolates from 1998 to 1999 (n=18) and 1999 to 2000 (n=11) were closer to A/Sydney/5/97. The mean number of amino acid differences for the 33 A/Sydney/5/97-like isolates compared with the homologous vaccine strain was 6.3 (1.9%), 9.2 (2.8%) and 13.6 (4.1%) for those collected in 1997-1998, 1998-1999, and 1999-2000, respectively. Phylogenetic analysis confirmed that a progressive drift occurred among our A/H3N2 influenza isolates over the last three flu seasons. Furthermore, it revealed that isolates collected during the last two flu seasons were in fact more related to A/Panama/2007/99 (2000-2001 vaccine strain) than to A/Sydney/5/97. Our studies suggest that molecular analysis of the HA1 gene should complement the HI assay for a more accurate analysis of influenza A virus drift.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hardy</LastName><ForeName>I</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Infectious Disease Research Center, Centre Hospitalier Universitaire de Québec and Laval University, Pavillon, CHUL RC-709, 2705 blvd Laurier, Québec, GIV 4G2, Sainte-Foy, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Coulthart</LastName><ForeName>M B</ForeName><Initials>MB</Initials></Author><Author ValidYN="Y"><LastName>Goyette</LastName><ForeName>N</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Boivin</LastName><ForeName>G</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Virus Res</MedlineTA><NlmUniqueID>8410979</NlmUniqueID><ISSNLinking>0168-1702</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004279">DNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019267">Hemagglutinin Glycoproteins, Influenza Virus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019143" MajorTopicYN="Y">Evolution, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005814" MajorTopicYN="N">Genes, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006385" MajorTopicYN="N">Hemagglutination Inhibition Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019267" MajorTopicYN="N">Hemagglutinin Glycoproteins, Influenza Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053122" MajorTopicYN="Y">Influenza A Virus, H3N2 Subtype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009980" MajorTopicYN="N">Influenza A virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011792" MajorTopicYN="N" Type="Geographic">Quebec</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2001</Year><Month>7</Month><Day>14</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>10</Month><Day>26</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2001</Year><Month>7</Month><Day>14</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11451491</ArticleId><ArticleId IdType="pii">S0168-1702(01)00269-6</ArticleId><ArticleId IdType="doi">10.1016/s0168-1702(01)00269-6</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/GrippeCanadaV4/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A77 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 000A77 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= GrippeCanadaV4
|flux= Main
|étape= Curation
|type= RBID
|clé= pubmed:11451491
|texte= Molecular evolution of influenza A/H3N2 viruses in the province of Québec (Canada) during the 1997-2000 period.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:11451491" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \
| NlmPubMed2Wicri -a GrippeCanadaV4
| This area was generated with Dilib version V0.6.35. |  |