Isolation and characterisation of segment 1 of the infectious salmon anaemia virus genome.
Identifieur interne : 000941 ( Main/Exploration ); précédent : 000940; suivant : 000942Isolation and characterisation of segment 1 of the infectious salmon anaemia virus genome.
Auteurs : M. Snow [Royaume-Uni] ; R. Ritchie ; O. Arnaud ; S. Villoing ; V. Aspehaug ; C O CunninghamSource :
- Virus research [ 0168-1702 ] ; 2003.
Descripteurs français
- KwdFr :
- ADN viral (génétique), Animaux, Canada, Données de séquences moléculaires, Génome viral, Infections à Orthomyxoviridae (médecine vétérinaire), Infections à Orthomyxoviridae (virologie), Lignée cellulaire, Maladies des poissons (virologie), Orthomyxoviridae (génétique), Orthomyxoviridae (isolement et purification), Orthomyxoviridae (pathogénicité), Protéines de fusion recombinantes (génétique), Protéines de fusion recombinantes (métabolisme), Protéines virales (génétique), Protéines virales (métabolisme), RNA replicase, RT-PCR, Saumon, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique, Transfection, Écosse.
- MESH :
- génétique : ADN viral, Orthomyxoviridae, Protéines de fusion recombinantes, Protéines virales.
- isolement et purification : Orthomyxoviridae.
- médecine vétérinaire : Infections à Orthomyxoviridae.
- métabolisme : Protéines de fusion recombinantes, Protéines virales.
- pathogénicité : Orthomyxoviridae.
- virologie : Infections à Orthomyxoviridae, Maladies des poissons.
- Animaux, Canada, Données de séquences moléculaires, Génome viral, Lignée cellulaire, RNA replicase, RT-PCR, Saumon, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique, Transfection, Écosse.
- Wicri :
- geographic : Canada.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Canada, Cell Line, DNA, Viral (genetics), Fish Diseases (virology), Genome, Viral, Molecular Sequence Data, Orthomyxoviridae (genetics), Orthomyxoviridae (isolation & purification), Orthomyxoviridae (pathogenicity), Orthomyxoviridae Infections (veterinary), Orthomyxoviridae Infections (virology), RNA Replicase, Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (metabolism), Reverse Transcriptase Polymerase Chain Reaction, Salmon, Scotland, Sequence Homology, Amino Acid, Transfection, Viral Proteins (genetics), Viral Proteins (metabolism).
- MESH :
- chemical , genetics : DNA, Viral, Recombinant Fusion Proteins, Viral Proteins.
- chemical , metabolism : Recombinant Fusion Proteins, Viral Proteins.
- geographic : Canada, RNA Replicase, Scotland.
- genetics : Orthomyxoviridae.
- isolation & purification : Orthomyxoviridae.
- pathogenicity : Orthomyxoviridae.
- veterinary : Orthomyxoviridae Infections.
- virology : Fish Diseases, Orthomyxoviridae Infections.
- Amino Acid Sequence, Animals, Base Sequence, Cell Line, Genome, Viral, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Salmon, Sequence Homology, Amino Acid, Transfection.
Abstract
The isolation and characterisation of the largest genomic segment of infectious salmon anaemia virus (ISAV) is reported. Following identification of ISAV-specific clones from a cDNA library, a rapid amplification of cDNA ends-PCR strategy was designed to obtain the sequence of the full length mRNA transcript. The full length open reading frame (ORF) of this gene was shown to be 2169 nucleotides in length, encoding a putative protein of 722 aa. This sequence was demonstrated by RT-PCR to be specific to ISAV-infected cell cultures. The start codon of this ORF was preceded by the ISAV consensus sequence 5' GCTAAGA 3' indicating the full 5' end of the gene to have been obtained. Based on protein size and amino acid composition, this protein was shown to be similar to the PB2 protein of other orthomyxoviruses. Furthermore, a bipartite nuclear localisation signal was identified in the C-terminus of the protein as is found on all of the influenza virus P proteins. Expression of the putative PB2 as a green fluorescent marker protein-fusion protein confirmed that this protein exhibited nuclear localisation in a fish cell line. Sequences of the ISAV segment 1 gene were obtained from Scottish, Norwegian and Canadian ISAV isolates. Analyses confirmed the close genetic relationship between Norwegian and Scottish ISAV and indicated that this segment was among the most conserved of the ISAV genes identified to date. Thus, this evidence strongly suggests that the genomic segment 1 of ISAV encodes a polymerase protein which is thought to be analagous in function to the PB2 protein of influenza viruses.
DOI: 10.1016/s0168-1702(02)00322-2
PubMed: 12606081
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Isolation and characterisation of segment 1 of the infectious salmon anaemia virus genome.</title><author><name sortKey="Snow, M" sort="Snow, M" uniqKey="Snow M" first="M" last="Snow">M. Snow</name><affiliation wicri:level="1"><nlm:affiliation>FRS Marine Laboratory, PO Box 101, Victoria Road, Aberdeen AB11 9DB, Scotland, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>FRS Marine Laboratory, PO Box 101, Victoria Road, Aberdeen AB11 9DB, Scotland</wicri:regionArea><wicri:noRegion>Scotland</wicri:noRegion></affiliation></author><author><name sortKey="Ritchie, R" sort="Ritchie, R" uniqKey="Ritchie R" first="R" last="Ritchie">R. Ritchie</name></author><author><name sortKey="Arnaud, O" sort="Arnaud, O" uniqKey="Arnaud O" first="O" last="Arnaud">O. Arnaud</name></author><author><name sortKey="Villoing, S" sort="Villoing, S" uniqKey="Villoing S" first="S" last="Villoing">S. Villoing</name></author><author><name sortKey="Aspehaug, V" sort="Aspehaug, V" uniqKey="Aspehaug V" first="V" last="Aspehaug">V. Aspehaug</name></author><author><name sortKey="Cunningham, C O" sort="Cunningham, C O" uniqKey="Cunningham C" first="C O" last="Cunningham">C O Cunningham</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12606081</idno><idno type="pmid">12606081</idno><idno type="doi">10.1016/s0168-1702(02)00322-2</idno><idno type="wicri:Area/Main/Corpus">000A28</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000A28</idno><idno type="wicri:Area/Main/Curation">000A28</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000A28</idno><idno type="wicri:Area/Main/Exploration">000A28</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Isolation and characterisation of segment 1 of the infectious salmon anaemia virus genome.</title><author><name sortKey="Snow, M" sort="Snow, M" uniqKey="Snow M" first="M" last="Snow">M. Snow</name><affiliation wicri:level="1"><nlm:affiliation>FRS Marine Laboratory, PO Box 101, Victoria Road, Aberdeen AB11 9DB, Scotland, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>FRS Marine Laboratory, PO Box 101, Victoria Road, Aberdeen AB11 9DB, Scotland</wicri:regionArea><wicri:noRegion>Scotland</wicri:noRegion></affiliation></author><author><name sortKey="Ritchie, R" sort="Ritchie, R" uniqKey="Ritchie R" first="R" last="Ritchie">R. Ritchie</name></author><author><name sortKey="Arnaud, O" sort="Arnaud, O" uniqKey="Arnaud O" first="O" last="Arnaud">O. Arnaud</name></author><author><name sortKey="Villoing, S" sort="Villoing, S" uniqKey="Villoing S" first="S" last="Villoing">S. Villoing</name></author><author><name sortKey="Aspehaug, V" sort="Aspehaug, V" uniqKey="Aspehaug V" first="V" last="Aspehaug">V. Aspehaug</name></author><author><name sortKey="Cunningham, C O" sort="Cunningham, C O" uniqKey="Cunningham C" first="C O" last="Cunningham">C O Cunningham</name></author></analytic><series><title level="j">Virus research</title><idno type="ISSN">0168-1702</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Canada</term><term>Cell Line</term><term>DNA, Viral (genetics)</term><term>Fish Diseases (virology)</term><term>Genome, Viral</term><term>Molecular Sequence Data</term><term>Orthomyxoviridae (genetics)</term><term>Orthomyxoviridae (isolation & purification)</term><term>Orthomyxoviridae (pathogenicity)</term><term>Orthomyxoviridae Infections (veterinary)</term><term>Orthomyxoviridae Infections (virology)</term><term>RNA Replicase</term><term>Recombinant Fusion Proteins (genetics)</term><term>Recombinant Fusion Proteins (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Salmon</term><term>Scotland</term><term>Sequence Homology, Amino Acid</term><term>Transfection</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term><term>Animaux</term><term>Canada</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Infections à Orthomyxoviridae (médecine vétérinaire)</term><term>Infections à Orthomyxoviridae (virologie)</term><term>Lignée cellulaire</term><term>Maladies des poissons (virologie)</term><term>Orthomyxoviridae (génétique)</term><term>Orthomyxoviridae (isolement et purification)</term><term>Orthomyxoviridae (pathogénicité)</term><term>Protéines de fusion recombinantes (génétique)</term><term>Protéines de fusion recombinantes (métabolisme)</term><term>Protéines virales (génétique)</term><term>Protéines virales (métabolisme)</term><term>RNA replicase</term><term>RT-PCR</term><term>Saumon</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Transfection</term><term>Écosse</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term><term>Recombinant Fusion Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Recombinant Fusion Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Canada</term><term>RNA Replicase</term><term>Scotland</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term><term>Orthomyxoviridae</term><term>Protéines de fusion recombinantes</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines de fusion recombinantes</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à Orthomyxoviridae</term><term>Maladies des poissons</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Fish Diseases</term><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cell Line</term><term>Genome, Viral</term><term>Molecular Sequence Data</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Salmon</term><term>Sequence Homology, Amino Acid</term><term>Transfection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Canada</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Lignée cellulaire</term><term>RNA replicase</term><term>RT-PCR</term><term>Saumon</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Transfection</term><term>Écosse</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Canada</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The isolation and characterisation of the largest genomic segment of infectious salmon anaemia virus (ISAV) is reported. Following identification of ISAV-specific clones from a cDNA library, a rapid amplification of cDNA ends-PCR strategy was designed to obtain the sequence of the full length mRNA transcript. The full length open reading frame (ORF) of this gene was shown to be 2169 nucleotides in length, encoding a putative protein of 722 aa. This sequence was demonstrated by RT-PCR to be specific to ISAV-infected cell cultures. The start codon of this ORF was preceded by the ISAV consensus sequence 5' GCTAAGA 3' indicating the full 5' end of the gene to have been obtained. Based on protein size and amino acid composition, this protein was shown to be similar to the PB2 protein of other orthomyxoviruses. Furthermore, a bipartite nuclear localisation signal was identified in the C-terminus of the protein as is found on all of the influenza virus P proteins. Expression of the putative PB2 as a green fluorescent marker protein-fusion protein confirmed that this protein exhibited nuclear localisation in a fish cell line. Sequences of the ISAV segment 1 gene were obtained from Scottish, Norwegian and Canadian ISAV isolates. Analyses confirmed the close genetic relationship between Norwegian and Scottish ISAV and indicated that this segment was among the most conserved of the ISAV genes identified to date. Thus, this evidence strongly suggests that the genomic segment 1 of ISAV encodes a polymerase protein which is thought to be analagous in function to the PB2 protein of influenza viruses.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12606081</PMID><DateCompleted><Year>2003</Year><Month>05</Month><Day>27</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0168-1702</ISSN><JournalIssue CitedMedium="Print"><Volume>92</Volume><Issue>1</Issue><PubDate><Year>2003</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Virus research</Title><ISOAbbreviation>Virus Res.</ISOAbbreviation></Journal><ArticleTitle>Isolation and characterisation of segment 1 of the infectious salmon anaemia virus genome.</ArticleTitle><Pagination><MedlinePgn>99-105</MedlinePgn></Pagination><Abstract><AbstractText>The isolation and characterisation of the largest genomic segment of infectious salmon anaemia virus (ISAV) is reported. Following identification of ISAV-specific clones from a cDNA library, a rapid amplification of cDNA ends-PCR strategy was designed to obtain the sequence of the full length mRNA transcript. The full length open reading frame (ORF) of this gene was shown to be 2169 nucleotides in length, encoding a putative protein of 722 aa. This sequence was demonstrated by RT-PCR to be specific to ISAV-infected cell cultures. The start codon of this ORF was preceded by the ISAV consensus sequence 5' GCTAAGA 3' indicating the full 5' end of the gene to have been obtained. Based on protein size and amino acid composition, this protein was shown to be similar to the PB2 protein of other orthomyxoviruses. Furthermore, a bipartite nuclear localisation signal was identified in the C-terminus of the protein as is found on all of the influenza virus P proteins. Expression of the putative PB2 as a green fluorescent marker protein-fusion protein confirmed that this protein exhibited nuclear localisation in a fish cell line. Sequences of the ISAV segment 1 gene were obtained from Scottish, Norwegian and Canadian ISAV isolates. Analyses confirmed the close genetic relationship between Norwegian and Scottish ISAV and indicated that this segment was among the most conserved of the ISAV genes identified to date. Thus, this evidence strongly suggests that the genomic segment 1 of ISAV encodes a polymerase protein which is thought to be analagous in function to the PB2 protein of influenza viruses.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Snow</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>FRS Marine Laboratory, PO Box 101, Victoria Road, Aberdeen AB11 9DB, Scotland, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ritchie</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Arnaud</LastName><ForeName>O</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Villoing</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Aspehaug</LastName><ForeName>V</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Cunningham</LastName><ForeName>C O</ForeName><Initials>CO</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Virus Res</MedlineTA><NlmUniqueID>8410979</NlmUniqueID><ISSNLinking>0168-1702</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004279">DNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C057027">PB2 protein, Influenzavirus A</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.7.48</RegistryNumber><NameOfSubstance UI="D012324">RNA Replicase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002170" MajorTopicYN="N" Type="Geographic">Canada</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005393" MajorTopicYN="N">Fish Diseases</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016679" MajorTopicYN="Y">Genome, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009975" MajorTopicYN="N">Orthomyxoviridae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009976" MajorTopicYN="N">Orthomyxoviridae Infections</DescriptorName><QualifierName UI="Q000662" MajorTopicYN="N">veterinary</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012324" MajorTopicYN="N">RNA Replicase</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012474" MajorTopicYN="N">Salmon</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012606" MajorTopicYN="N" Type="Geographic">Scotland</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014162" MajorTopicYN="N">Transfection</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>2</Month><Day>28</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>5</Month><Day>28</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>2</Month><Day>28</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12606081</ArticleId><ArticleId IdType="pii">S0168170202003222</ArticleId><ArticleId IdType="doi">10.1016/s0168-1702(02)00322-2</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><noCountry><name sortKey="Arnaud, O" sort="Arnaud, O" uniqKey="Arnaud O" first="O" last="Arnaud">O. Arnaud</name><name sortKey="Aspehaug, V" sort="Aspehaug, V" uniqKey="Aspehaug V" first="V" last="Aspehaug">V. Aspehaug</name><name sortKey="Cunningham, C O" sort="Cunningham, C O" uniqKey="Cunningham C" first="C O" last="Cunningham">C O Cunningham</name><name sortKey="Ritchie, R" sort="Ritchie, R" uniqKey="Ritchie R" first="R" last="Ritchie">R. Ritchie</name><name sortKey="Villoing, S" sort="Villoing, S" uniqKey="Villoing S" first="S" last="Villoing">S. Villoing</name></noCountry><country name="Royaume-Uni"><noRegion><name sortKey="Snow, M" sort="Snow, M" uniqKey="Snow M" first="M" last="Snow">M. Snow</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/GrippeCanadaV3/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000941 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000941 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= GrippeCanadaV3
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:12606081
|texte= Isolation and characterisation of segment 1 of the infectious salmon anaemia virus genome.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:12606081" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a GrippeCanadaV3
| This area was generated with Dilib version V0.6.35. |  |