Prospective evaluation of Coris Influ-A&B Respi-Strip and of BinaxNOW Influenza A&B assay against viral culture and real-time PCR assay for detection of 2009 pandemic influenza A/H1N1v in Belgian patients.
Identifieur interne : 000054 ( Main/Corpus ); précédent : 000053; suivant : 000055Prospective evaluation of Coris Influ-A&B Respi-Strip and of BinaxNOW Influenza A&B assay against viral culture and real-time PCR assay for detection of 2009 pandemic influenza A/H1N1v in Belgian patients.
Auteurs : M. Reynders ; M. De Foor ; Y. Maaroufi ; I. Thomas ; A. Vergison ; S. Debulpaep ; O. Vandenberg ; F. CrokaertSource :
- Acta clinica Belgica [ 1784-3286 ]
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Belgium, Child, Child, Preschool, Chromatography, Affinity, Humans, Infant, Influenza A Virus, H1N1 Subtype, Influenza, Human (diagnosis), Male, Middle Aged, Prospective Studies, Reagent Kits, Diagnostic, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Young Adult.
- MESH :
- chemical : Reagent Kits, Diagnostic.
- geographic : Belgium.
- diagnosis : Influenza, Human.
- Adolescent, Adult, Aged, Child, Child, Preschool, Chromatography, Affinity, Humans, Infant, Influenza A Virus, H1N1 Subtype, Male, Middle Aged, Prospective Studies, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Young Adult.
Abstract
PURPOSE
Evaluation of the performance of two rapid (15') antigen detection tests (RAT), BinaxNOW Influenza A&B and Coris Influ-A&B Respi-Strip for the detection of A(H1N1)v2009.
STUDY DESIGN
Between July 2009 and November 2009, 4105 respiratory specimens from patients with influenza-like illness attending seven public hospitals in Brussels were prospectively examined by two immunochromatographic RAT, followed by viral culture and/or specific real-time RT-PCR.
RESULTS
Samples consisted predominantly of nasopharyngeal aspirates (NPA-41%), nasopharyngeal (NPS-37%) and throat swabs (TS-14%). The sensitivity and specificity of Coris RAT and BinaxNOW RAT were 36.6% and 99.7%, and 47% and 98.7% respectively compared to culture; and 33.7% and 99.6%; and 46.5% and 98.8% compared to RT-PCR. Significant differences in sensitivity could be observed when splitting up the samples by sample type and patient's age. NPA gave by far the highest sensitivities: 51.1- 62% for Coris compared to culture and 62.6-78.4% for BinaxNOW. Sensitivities in paediatric NPS varied less between different hospitals (34-41.9%) being still much higher than in adult NPS (11.4-20%). TS resulted in unsatisfactory results: 13% sensitivity in children and 10.5% in adults.
CONCLUSIONS
Both RAT showed excellent specificities, but insufficient sensitivities. Consequently, negative results should be confirmed. NPA are clearly superior to NPS orTS, and they stay the sample of choice for viral diagnosis.
DOI: 10.2143/ACB.67.2.2062638
PubMed: 22712164
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pubmed:22712164Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Prospective evaluation of Coris Influ-A&B Respi-Strip and of BinaxNOW Influenza A&B assay against viral culture and real-time PCR assay for detection of 2009 pandemic influenza A/H1N1v in Belgian patients.</title><author><name sortKey="Reynders, M" sort="Reynders, M" uniqKey="Reynders M" first="M" last="Reynders">M. Reynders</name><affiliation><nlm:affiliation>Laboratoire de Microbiologie, Secteur de Virologie, Centre Hospitalier Universitaire Saint-Pierre, Bruxelles, Belgium. marijke.reynders@azsintjan.be</nlm:affiliation></affiliation></author><author><name sortKey="De Foor, M" sort="De Foor, M" uniqKey="De Foor M" first="M" last="De Foor">M. De Foor</name></author><author><name sortKey="Maaroufi, Y" sort="Maaroufi, Y" uniqKey="Maaroufi Y" first="Y" last="Maaroufi">Y. Maaroufi</name></author><author><name sortKey="Thomas, I" sort="Thomas, I" uniqKey="Thomas I" first="I" last="Thomas">I. Thomas</name></author><author><name sortKey="Vergison, A" sort="Vergison, A" uniqKey="Vergison A" first="A" last="Vergison">A. Vergison</name></author><author><name sortKey="Debulpaep, S" sort="Debulpaep, S" uniqKey="Debulpaep S" first="S" last="Debulpaep">S. Debulpaep</name></author><author><name sortKey="Vandenberg, O" sort="Vandenberg, O" uniqKey="Vandenberg O" first="O" last="Vandenberg">O. Vandenberg</name></author><author><name sortKey="Crokaert, F" sort="Crokaert, F" uniqKey="Crokaert F" first="F" last="Crokaert">F. Crokaert</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>2012 Mar-Apr</MedlineDate></PubDate></date><idno type="RBID">pubmed:22712164</idno><idno type="pmid">22712164</idno><idno type="doi">10.2143/ACB.67.2.2062638</idno><idno type="wicri:Area/Main/Corpus">000054</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000054</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Prospective evaluation of Coris Influ-A&B Respi-Strip and of BinaxNOW Influenza A&B assay against viral culture and real-time PCR assay for detection of 2009 pandemic influenza A/H1N1v in Belgian patients.</title><author><name sortKey="Reynders, M" sort="Reynders, M" uniqKey="Reynders M" first="M" last="Reynders">M. Reynders</name><affiliation><nlm:affiliation>Laboratoire de Microbiologie, Secteur de Virologie, Centre Hospitalier Universitaire Saint-Pierre, Bruxelles, Belgium. marijke.reynders@azsintjan.be</nlm:affiliation></affiliation></author><author><name sortKey="De Foor, M" sort="De Foor, M" uniqKey="De Foor M" first="M" last="De Foor">M. De Foor</name></author><author><name sortKey="Maaroufi, Y" sort="Maaroufi, Y" uniqKey="Maaroufi Y" first="Y" last="Maaroufi">Y. Maaroufi</name></author><author><name sortKey="Thomas, I" sort="Thomas, I" uniqKey="Thomas I" first="I" last="Thomas">I. Thomas</name></author><author><name sortKey="Vergison, A" sort="Vergison, A" uniqKey="Vergison A" first="A" last="Vergison">A. Vergison</name></author><author><name sortKey="Debulpaep, S" sort="Debulpaep, S" uniqKey="Debulpaep S" first="S" last="Debulpaep">S. Debulpaep</name></author><author><name sortKey="Vandenberg, O" sort="Vandenberg, O" uniqKey="Vandenberg O" first="O" last="Vandenberg">O. Vandenberg</name></author><author><name sortKey="Crokaert, F" sort="Crokaert, F" uniqKey="Crokaert F" first="F" last="Crokaert">F. Crokaert</name></author></analytic><series><title level="j">Acta clinica Belgica</title><idno type="ISSN">1784-3286</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Belgium</term><term>Child</term><term>Child, Preschool</term><term>Chromatography, Affinity</term><term>Humans</term><term>Infant</term><term>Influenza A Virus, H1N1 Subtype</term><term>Influenza, Human (diagnosis)</term><term>Male</term><term>Middle Aged</term><term>Prospective Studies</term><term>Reagent Kits, Diagnostic</term><term>Real-Time Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Reagent Kits, Diagnostic</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Belgium</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Child</term><term>Child, Preschool</term><term>Chromatography, Affinity</term><term>Humans</term><term>Infant</term><term>Influenza A Virus, H1N1 Subtype</term><term>Male</term><term>Middle Aged</term><term>Prospective Studies</term><term>Real-Time Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>PURPOSE</b></p><p>Evaluation of the performance of two rapid (15') antigen detection tests (RAT), BinaxNOW Influenza A&B and Coris Influ-A&B Respi-Strip for the detection of A(H1N1)v2009.</p></div><div type="abstract" xml:lang="en"><p><b>STUDY DESIGN</b></p><p>Between July 2009 and November 2009, 4105 respiratory specimens from patients with influenza-like illness attending seven public hospitals in Brussels were prospectively examined by two immunochromatographic RAT, followed by viral culture and/or specific real-time RT-PCR.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Samples consisted predominantly of nasopharyngeal aspirates (NPA-41%), nasopharyngeal (NPS-37%) and throat swabs (TS-14%). The sensitivity and specificity of Coris RAT and BinaxNOW RAT were 36.6% and 99.7%, and 47% and 98.7% respectively compared to culture; and 33.7% and 99.6%; and 46.5% and 98.8% compared to RT-PCR. Significant differences in sensitivity could be observed when splitting up the samples by sample type and patient's age. NPA gave by far the highest sensitivities: 51.1- 62% for Coris compared to culture and 62.6-78.4% for BinaxNOW. Sensitivities in paediatric NPS varied less between different hospitals (34-41.9%) being still much higher than in adult NPS (11.4-20%). TS resulted in unsatisfactory results: 13% sensitivity in children and 10.5% in adults.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Both RAT showed excellent specificities, but insufficient sensitivities. Consequently, negative results should be confirmed. NPA are clearly superior to NPS orTS, and they stay the sample of choice for viral diagnosis.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22712164</PMID><DateCompleted><Year>2012</Year><Month>07</Month><Day>24</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1784-3286</ISSN><JournalIssue CitedMedium="Print"><Volume>67</Volume><Issue>2</Issue><PubDate><MedlineDate>2012 Mar-Apr</MedlineDate></PubDate></JournalIssue><Title>Acta clinica Belgica</Title><ISOAbbreviation>Acta Clin Belg</ISOAbbreviation></Journal><ArticleTitle>Prospective evaluation of Coris Influ-A&B Respi-Strip and of BinaxNOW Influenza A&B assay against viral culture and real-time PCR assay for detection of 2009 pandemic influenza A/H1N1v in Belgian patients.</ArticleTitle><Pagination><MedlinePgn>94-8</MedlinePgn></Pagination><Abstract><AbstractText Label="PURPOSE" NlmCategory="OBJECTIVE">Evaluation of the performance of two rapid (15') antigen detection tests (RAT), BinaxNOW Influenza A&B and Coris Influ-A&B Respi-Strip for the detection of A(H1N1)v2009.</AbstractText><AbstractText Label="STUDY DESIGN" NlmCategory="METHODS">Between July 2009 and November 2009, 4105 respiratory specimens from patients with influenza-like illness attending seven public hospitals in Brussels were prospectively examined by two immunochromatographic RAT, followed by viral culture and/or specific real-time RT-PCR.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Samples consisted predominantly of nasopharyngeal aspirates (NPA-41%), nasopharyngeal (NPS-37%) and throat swabs (TS-14%). The sensitivity and specificity of Coris RAT and BinaxNOW RAT were 36.6% and 99.7%, and 47% and 98.7% respectively compared to culture; and 33.7% and 99.6%; and 46.5% and 98.8% compared to RT-PCR. Significant differences in sensitivity could be observed when splitting up the samples by sample type and patient's age. NPA gave by far the highest sensitivities: 51.1- 62% for Coris compared to culture and 62.6-78.4% for BinaxNOW. Sensitivities in paediatric NPS varied less between different hospitals (34-41.9%) being still much higher than in adult NPS (11.4-20%). TS resulted in unsatisfactory results: 13% sensitivity in children and 10.5% in adults.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Both RAT showed excellent specificities, but insufficient sensitivities. Consequently, negative results should be confirmed. NPA are clearly superior to NPS orTS, and they stay the sample of choice for viral diagnosis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Reynders</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratoire de Microbiologie, Secteur de Virologie, Centre Hospitalier Universitaire Saint-Pierre, Bruxelles, Belgium. marijke.reynders@azsintjan.be</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>De Foor</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Maaroufi</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Thomas</LastName><ForeName>I</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Vergison</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Debulpaep</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Vandenberg</LastName><ForeName>O</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Crokaert</LastName><ForeName>F</ForeName><Initials>F</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D016448">Multicenter Study</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Acta Clin Belg</MedlineTA><NlmUniqueID>0370306</NlmUniqueID><ISSNLinking>1784-3286</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011933">Reagent Kits, Diagnostic</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001530" MajorTopicYN="N" Type="Geographic">Belgium</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002648" MajorTopicYN="N">Child</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002675" MajorTopicYN="N">Child, Preschool</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002846" MajorTopicYN="N">Chromatography, Affinity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007223" MajorTopicYN="N">Infant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053118" MajorTopicYN="Y">Influenza A Virus, H1N1 Subtype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011446" MajorTopicYN="N">Prospective Studies</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011933" MajorTopicYN="Y">Reagent Kits, Diagnostic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055815" MajorTopicYN="N">Young Adult</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>6</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>6</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>7</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22712164</ArticleId><ArticleId IdType="doi">10.2143/ACB.67.2.2062638</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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