Occurrence and severity of lung lesions in slaughter pigs vaccinated against Mycoplasma hyopneumoniae with different strategies.
Identifieur interne : 000171 ( Main/Corpus ); précédent : 000170; suivant : 000172Occurrence and severity of lung lesions in slaughter pigs vaccinated against Mycoplasma hyopneumoniae with different strategies.
Auteurs : Sonja Hillen ; Stephan Von Berg ; Kernt Köhler ; Manfred Reinacher ; Hermann Willems ; Gerald ReinerSource :
- Preventive veterinary medicine [ 1873-1716 ] ; 2014.
English descriptors
- KwdEn :
- Animals (MeSH), Bacteria (isolation & purification), Bacterial Load (veterinary), Bacterial Vaccines (administration & dosage), Bacterial Vaccines (pharmacology), Female (MeSH), Germany (epidemiology), Lung (pathology), Male (MeSH), Mycoplasma hyopneumoniae (isolation & purification), Pneumonia of Swine, Mycoplasmal (epidemiology), Pneumonia of Swine, Mycoplasmal (pathology), Pneumonia of Swine, Mycoplasmal (prevention & control), Pneumonia of Swine, Mycoplasmal (virology), Prevalence (MeSH), Real-Time Polymerase Chain Reaction (veterinary), Swine (MeSH), Vaccination (veterinary), Viruses (isolation & purification).
- MESH :
- chemical , administration & dosage : Bacterial Vaccines.
- geographic , epidemiology : Germany.
- epidemiology : Pneumonia of Swine, Mycoplasmal.
- isolation & purification : Bacteria, Mycoplasma hyopneumoniae, Viruses.
- pathology : Lung, Pneumonia of Swine, Mycoplasmal.
- chemical , pharmacology : Bacterial Vaccines.
- prevention & control : Pneumonia of Swine, Mycoplasmal.
- veterinary : Bacterial Load, Real-Time Polymerase Chain Reaction, Vaccination.
- virology : Pneumonia of Swine, Mycoplasmal.
- Animals, Female, Male, Prevalence, Swine.
Abstract
Different vaccination strategies against Mycoplasma hyopneumoniae have been adopted worldwide. Reports from the field indicate varying levels of protection among currently available vaccines. The goal of the present study was to compare the efficacies of three widespread commercial vaccination strategies against M. hyopneumoniae under field conditions. 20 farms were included. 14 farms used different single dose vaccines (vaccine 1 [V1], 8 herds; vaccine 2 [V2], 6 herds); another 6 farms (V3) used a two dose vaccination strategy. Gross lesions of 854 lungs and histopathology from 140 lungs were quantified, and a quantitative PCR was applied to detect M. hyopneumoniae and porcine circovirus 2 (PCV2) DNA in lung tissue (n=140). In addition, porcine reproductive and respiratory disease virus (PRRSV), swine influenza virus (SIV), Actinobacillus pleuropneumoniae, Haemophilus parasuis and Pasteurella multocida were tested by qualitative PCR. 53% of lungs were positive for M. hyopneumoniae. 55.9% of lungs showed macroscopic enzootic pneumonia (EP)-like lesions. Lung lesion scores (P<0.001) and M. hyopneumoniae-loads (P<0.008) differed significantly among the vaccination groups, with the most severe cases and highest amounts occurring in V1. Histological alterations differed (P<0.001) between V1 and V3. Lung lesion scores and histopathological changes were significantly correlated, with prevalence and load of M. hyopneumoniae indicating that the applied diagnostic tools are valuable in confirming the prevalence and severity of M. hyopneumoniae infections. Comparing different vaccination strategies against M. hyopneumoniae indicates varying levels of protection. M. hyopneumoniae is still a major problem despite the widely applied vaccination.
DOI: 10.1016/j.prevetmed.2013.12.012
PubMed: 24485705
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pubmed:24485705Le document en format XML
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Electronic address: gerald.reiner@vetmed.uni-giessen.de.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24485705</idno><idno type="pmid">24485705</idno><idno type="doi">10.1016/j.prevetmed.2013.12.012</idno><idno type="wicri:Area/Main/Corpus">000171</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000171</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Occurrence and severity of lung lesions in slaughter pigs vaccinated against Mycoplasma hyopneumoniae with different strategies.</title><author><name sortKey="Hillen, Sonja" sort="Hillen, Sonja" uniqKey="Hillen S" first="Sonja" last="Hillen">Sonja Hillen</name><affiliation><nlm:affiliation>Department of Clinical Sciences, Justus-Liebig-University Giessen, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Von Berg, Stephan" sort="Von Berg, Stephan" uniqKey="Von Berg S" first="Stephan" last="Von Berg">Stephan Von Berg</name><affiliation><nlm:affiliation>MSD Animal Health, Munich, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Kohler, Kernt" sort="Kohler, Kernt" uniqKey="Kohler K" first="Kernt" last="Köhler">Kernt Köhler</name><affiliation><nlm:affiliation>Institute for Veterinary Pathology, Justus-Liebig-University Giessen, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Reinacher, Manfred" sort="Reinacher, Manfred" uniqKey="Reinacher M" first="Manfred" last="Reinacher">Manfred Reinacher</name><affiliation><nlm:affiliation>Institute for Veterinary Pathology, Justus-Liebig-University Giessen, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Willems, Hermann" sort="Willems, Hermann" uniqKey="Willems H" first="Hermann" last="Willems">Hermann Willems</name><affiliation><nlm:affiliation>Department of Clinical Sciences, Justus-Liebig-University Giessen, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Reiner, Gerald" sort="Reiner, Gerald" uniqKey="Reiner G" first="Gerald" last="Reiner">Gerald Reiner</name><affiliation><nlm:affiliation>Department of Clinical Sciences, Justus-Liebig-University Giessen, Germany. Electronic address: gerald.reiner@vetmed.uni-giessen.de.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Preventive veterinary medicine</title><idno type="eISSN">1873-1716</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Bacteria (isolation & purification)</term><term>Bacterial Load (veterinary)</term><term>Bacterial Vaccines (administration & dosage)</term><term>Bacterial Vaccines (pharmacology)</term><term>Female (MeSH)</term><term>Germany (epidemiology)</term><term>Lung (pathology)</term><term>Male (MeSH)</term><term>Mycoplasma hyopneumoniae (isolation & purification)</term><term>Pneumonia of Swine, Mycoplasmal (epidemiology)</term><term>Pneumonia of Swine, Mycoplasmal (pathology)</term><term>Pneumonia of Swine, Mycoplasmal (prevention & control)</term><term>Pneumonia of Swine, Mycoplasmal (virology)</term><term>Prevalence (MeSH)</term><term>Real-Time Polymerase Chain Reaction (veterinary)</term><term>Swine (MeSH)</term><term>Vaccination (veterinary)</term><term>Viruses (isolation & purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Bacterial Vaccines</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Germany</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Pneumonia of Swine, Mycoplasmal</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacteria</term><term>Mycoplasma hyopneumoniae</term><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Lung</term><term>Pneumonia of Swine, Mycoplasmal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Bacterial Vaccines</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Pneumonia of Swine, Mycoplasmal</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Bacterial Load</term><term>Real-Time Polymerase Chain Reaction</term><term>Vaccination</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Pneumonia of Swine, Mycoplasmal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Male</term><term>Prevalence</term><term>Swine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Different vaccination strategies against Mycoplasma hyopneumoniae have been adopted worldwide. Reports from the field indicate varying levels of protection among currently available vaccines. The goal of the present study was to compare the efficacies of three widespread commercial vaccination strategies against M. hyopneumoniae under field conditions. 20 farms were included. 14 farms used different single dose vaccines (vaccine 1 [V1], 8 herds; vaccine 2 [V2], 6 herds); another 6 farms (V3) used a two dose vaccination strategy. Gross lesions of 854 lungs and histopathology from 140 lungs were quantified, and a quantitative PCR was applied to detect M. hyopneumoniae and porcine circovirus 2 (PCV2) DNA in lung tissue (n=140). In addition, porcine reproductive and respiratory disease virus (PRRSV), swine influenza virus (SIV), Actinobacillus pleuropneumoniae, Haemophilus parasuis and Pasteurella multocida were tested by qualitative PCR. 53% of lungs were positive for M. hyopneumoniae. 55.9% of lungs showed macroscopic enzootic pneumonia (EP)-like lesions. Lung lesion scores (P<0.001) and M. hyopneumoniae-loads (P<0.008) differed significantly among the vaccination groups, with the most severe cases and highest amounts occurring in V1. Histological alterations differed (P<0.001) between V1 and V3. Lung lesion scores and histopathological changes were significantly correlated, with prevalence and load of M. hyopneumoniae indicating that the applied diagnostic tools are valuable in confirming the prevalence and severity of M. hyopneumoniae infections. Comparing different vaccination strategies against M. hyopneumoniae indicates varying levels of protection. M. hyopneumoniae is still a major problem despite the widely applied vaccination. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24485705</PMID><DateCompleted><Year>2014</Year><Month>10</Month><Day>15</Day></DateCompleted><DateRevised><Year>2014</Year><Month>02</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-1716</ISSN><JournalIssue CitedMedium="Internet"><Volume>113</Volume><Issue>4</Issue><PubDate><Year>2014</Year><Month>Mar</Month><Day>01</Day></PubDate></JournalIssue><Title>Preventive veterinary medicine</Title><ISOAbbreviation>Prev. Vet. Med.</ISOAbbreviation></Journal><ArticleTitle>Occurrence and severity of lung lesions in slaughter pigs vaccinated against Mycoplasma hyopneumoniae with different strategies.</ArticleTitle><Pagination><MedlinePgn>580-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.prevetmed.2013.12.012</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0167-5877(13)00394-2</ELocationID><Abstract><AbstractText>Different vaccination strategies against Mycoplasma hyopneumoniae have been adopted worldwide. Reports from the field indicate varying levels of protection among currently available vaccines. The goal of the present study was to compare the efficacies of three widespread commercial vaccination strategies against M. hyopneumoniae under field conditions. 20 farms were included. 14 farms used different single dose vaccines (vaccine 1 [V1], 8 herds; vaccine 2 [V2], 6 herds); another 6 farms (V3) used a two dose vaccination strategy. Gross lesions of 854 lungs and histopathology from 140 lungs were quantified, and a quantitative PCR was applied to detect M. hyopneumoniae and porcine circovirus 2 (PCV2) DNA in lung tissue (n=140). In addition, porcine reproductive and respiratory disease virus (PRRSV), swine influenza virus (SIV), Actinobacillus pleuropneumoniae, Haemophilus parasuis and Pasteurella multocida were tested by qualitative PCR. 53% of lungs were positive for M. hyopneumoniae. 55.9% of lungs showed macroscopic enzootic pneumonia (EP)-like lesions. Lung lesion scores (P<0.001) and M. hyopneumoniae-loads (P<0.008) differed significantly among the vaccination groups, with the most severe cases and highest amounts occurring in V1. Histological alterations differed (P<0.001) between V1 and V3. Lung lesion scores and histopathological changes were significantly correlated, with prevalence and load of M. hyopneumoniae indicating that the applied diagnostic tools are valuable in confirming the prevalence and severity of M. hyopneumoniae infections. Comparing different vaccination strategies against M. hyopneumoniae indicates varying levels of protection. M. hyopneumoniae is still a major problem despite the widely applied vaccination. </AbstractText><CopyrightInformation>Copyright © 2014 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hillen</LastName><ForeName>Sonja</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Clinical Sciences, Justus-Liebig-University Giessen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>von Berg</LastName><ForeName>Stephan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>MSD Animal Health, Munich, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Köhler</LastName><ForeName>Kernt</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Institute for Veterinary Pathology, Justus-Liebig-University Giessen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reinacher</LastName><ForeName>Manfred</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute for Veterinary Pathology, Justus-Liebig-University Giessen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Willems</LastName><ForeName>Hermann</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Clinical Sciences, Justus-Liebig-University Giessen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reiner</LastName><ForeName>Gerald</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Clinical Sciences, Justus-Liebig-University Giessen, Germany. Electronic address: gerald.reiner@vetmed.uni-giessen.de.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>01</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Prev Vet Med</MedlineTA><NlmUniqueID>8217463</NlmUniqueID><ISSNLinking>0167-5877</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001428">Bacterial Vaccines</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058491" MajorTopicYN="N">Bacterial Load</DescriptorName><QualifierName UI="Q000662" MajorTopicYN="N">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001428" MajorTopicYN="N">Bacterial Vaccines</DescriptorName><QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005858" MajorTopicYN="N" Type="Geographic">Germany</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008168" MajorTopicYN="N">Lung</DescriptorName><QualifierName UI="Q000473" MajorTopicYN="Y">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045705" MajorTopicYN="N">Mycoplasma hyopneumoniae</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045729" MajorTopicYN="N">Pneumonia of Swine, Mycoplasmal</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="Y">epidemiology</QualifierName><QualifierName UI="Q000473" MajorTopicYN="Y">pathology</QualifierName><QualifierName UI="Q000517" MajorTopicYN="N">prevention & control</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015995" MajorTopicYN="N">Prevalence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000662" MajorTopicYN="N">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013552" MajorTopicYN="N">Swine</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014611" MajorTopicYN="N">Vaccination</DescriptorName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014780" MajorTopicYN="N">Viruses</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Mycoplasma hyopneumoniae</Keyword><Keyword MajorTopicYN="N">Swine</Keyword><Keyword MajorTopicYN="N">Vaccination</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>07</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>12</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>12</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>2</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>2</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>10</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24485705</ArticleId><ArticleId IdType="pii">S0167-5877(13)00394-2</ArticleId><ArticleId IdType="doi">10.1016/j.prevetmed.2013.12.012</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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