Cytokine production by cells adherent to regenerative membranes.
Identifieur interne : 003E95 ( PubMed/Corpus ); précédent : 003E94; suivant : 003E96Cytokine production by cells adherent to regenerative membranes.
Auteurs : R C Wakabayashi ; D K Iha ; J J Niu ; P W JohnsonSource :
- Journal of periodontal research [ 0022-3484 ] ; 1997.
English descriptors
- KwdEn :
- Adult, Aged, Alveolar Ridge Augmentation, Bone Regeneration, Calcification, Physiologic (genetics), Cell Adhesion, Cell Separation, Cytokines (biosynthesis), Dental Implants, Female, Fibroblasts (metabolism), Gene Expression, Gingiva (metabolism), Gingiva (pathology), Guided Tissue Regeneration, Periodontal, Humans, Interferon-gamma (biosynthesis), Interferon-gamma (genetics), Interleukin-1 (biosynthesis), Interleukin-1 (genetics), Interleukin-4 (biosynthesis), Interleukin-4 (genetics), Jaw, Edentulous (surgery), Male, Membranes, Artificial, Middle Aged, Periodontal Ligament (metabolism), Periodontal Ligament (pathology), Polymerase Chain Reaction, Polytetrafluoroethylene, RNA, Messenger (analysis), Transcription, Genetic.
- MESH :
- chemical , analysis : RNA, Messenger.
- chemical , biosynthesis : Cytokines, Interferon-gamma, Interleukin-1, Interleukin-4.
- genetics : Calcification, Physiologic, Interferon-gamma, Interleukin-1, Interleukin-4.
- metabolism : Fibroblasts, Gingiva, Periodontal Ligament.
- pathology : Gingiva, Periodontal Ligament.
- surgery : Jaw, Edentulous.
- Adult, Aged, Alveolar Ridge Augmentation, Bone Regeneration, Cell Adhesion, Cell Separation, Dental Implants, Female, Gene Expression, Guided Tissue Regeneration, Periodontal, Humans, Male, Membranes, Artificial, Middle Aged, Polymerase Chain Reaction, Polytetrafluoroethylene, Transcription, Genetic.
Abstract
Since cytokines play a critical role in tissue regeneration, we have assayed cytokine production by cells from tissue adherent to regenerative membranes. Cells were recovered from Gore-tex membranes in guided tissue regeneration (GTR) procedures to regenerate that attachment apparatus around teeth and from Gore-tex augmentation membranes (GTAM) used for guided gone regeneration (GBR) procedures in edentulous ridge augmentation with or without implant placement. Cells were screened for mineralized nodule formation in vitro to mRNA analysis to demonstrate that they could form mineralized tissue. Production in interleukin-1 alpha (IL-1 alpha) interleukin-1 beta (IL-1 beta), interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) was evaluated by reverse transcribed-polymerase chain reaction (RT-PCR) of mRNA from rescued regenerative cells, human gingival fibroblasts and periodontal ligament (PDL) cells. Both the gingival fibroblast and PDL cells isolates produced all 4 cytokines. However, the cell isolates from the regenerative membranes had various profiles of cytokine expression. Most GTR cell isolates were positive for all 4 cytokines. IL-1 beta was produced by all 6 GTR cell isolates but was not detected at the same number of cycles of RT-PCR amplification in any of the 6 GBR cell isolates. IL-1 beta transcripts were also not observed in cells derived from a direct biopsy of GBR tissue. Cells were recovered from unexposed GBR membranes did not produce detectable amounts of IFN-gamma, whereas cells recovered from exposed GBR and all GTR membranes produced IFN-gamma. These findings indicate that cells from regenerative tissues express different cytokines and that exposure to the tissue to the oral cavity during healing may modulate this expression.
PubMed: 9089488
Links to Exploration step
pubmed:9089488Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cytokine production by cells adherent to regenerative membranes.</title><author><name sortKey="Wakabayashi, R C" sort="Wakabayashi, R C" uniqKey="Wakabayashi R" first="R C" last="Wakabayashi">R C Wakabayashi</name><affiliation><nlm:affiliation>Department of Stomatology, School of Dentistry, University of California, San Francisco 94143-0650, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Iha, D K" sort="Iha, D K" uniqKey="Iha D" first="D K" last="Iha">D K Iha</name></author><author><name sortKey="Niu, J J" sort="Niu, J J" uniqKey="Niu J" first="J J" last="Niu">J J Niu</name></author><author><name sortKey="Johnson, P W" sort="Johnson, P W" uniqKey="Johnson P" first="P W" last="Johnson">P W Johnson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1997">1997</date><idno type="RBID">pubmed:9089488</idno><idno type="pmid">9089488</idno><idno type="wicri:Area/PubMed/Corpus">003E95</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003E95</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cytokine production by cells adherent to regenerative membranes.</title><author><name sortKey="Wakabayashi, R C" sort="Wakabayashi, R C" uniqKey="Wakabayashi R" first="R C" last="Wakabayashi">R C Wakabayashi</name><affiliation><nlm:affiliation>Department of Stomatology, School of Dentistry, University of California, San Francisco 94143-0650, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Iha, D K" sort="Iha, D K" uniqKey="Iha D" first="D K" last="Iha">D K Iha</name></author><author><name sortKey="Niu, J J" sort="Niu, J J" uniqKey="Niu J" first="J J" last="Niu">J J Niu</name></author><author><name sortKey="Johnson, P W" sort="Johnson, P W" uniqKey="Johnson P" first="P W" last="Johnson">P W Johnson</name></author></analytic><series><title level="j">Journal of periodontal research</title><idno type="ISSN">0022-3484</idno><imprint><date when="1997" type="published">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Aged</term><term>Alveolar Ridge Augmentation</term><term>Bone Regeneration</term><term>Calcification, Physiologic (genetics)</term><term>Cell Adhesion</term><term>Cell Separation</term><term>Cytokines (biosynthesis)</term><term>Dental Implants</term><term>Female</term><term>Fibroblasts (metabolism)</term><term>Gene Expression</term><term>Gingiva (metabolism)</term><term>Gingiva (pathology)</term><term>Guided Tissue Regeneration, Periodontal</term><term>Humans</term><term>Interferon-gamma (biosynthesis)</term><term>Interferon-gamma (genetics)</term><term>Interleukin-1 (biosynthesis)</term><term>Interleukin-1 (genetics)</term><term>Interleukin-4 (biosynthesis)</term><term>Interleukin-4 (genetics)</term><term>Jaw, Edentulous (surgery)</term><term>Male</term><term>Membranes, Artificial</term><term>Middle Aged</term><term>Periodontal Ligament (metabolism)</term><term>Periodontal Ligament (pathology)</term><term>Polymerase Chain Reaction</term><term>Polytetrafluoroethylene</term><term>RNA, Messenger (analysis)</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Cytokines</term><term>Interferon-gamma</term><term>Interleukin-1</term><term>Interleukin-4</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Calcification, Physiologic</term><term>Interferon-gamma</term><term>Interleukin-1</term><term>Interleukin-4</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fibroblasts</term><term>Gingiva</term><term>Periodontal Ligament</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Gingiva</term><term>Periodontal Ligament</term></keywords><keywords scheme="MESH" qualifier="surgery" xml:lang="en"><term>Jaw, Edentulous</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Aged</term><term>Alveolar Ridge Augmentation</term><term>Bone Regeneration</term><term>Cell Adhesion</term><term>Cell Separation</term><term>Dental Implants</term><term>Female</term><term>Gene Expression</term><term>Guided Tissue Regeneration, Periodontal</term><term>Humans</term><term>Male</term><term>Membranes, Artificial</term><term>Middle Aged</term><term>Polymerase Chain Reaction</term><term>Polytetrafluoroethylene</term><term>Transcription, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Since cytokines play a critical role in tissue regeneration, we have assayed cytokine production by cells from tissue adherent to regenerative membranes. Cells were recovered from Gore-tex membranes in guided tissue regeneration (GTR) procedures to regenerate that attachment apparatus around teeth and from Gore-tex augmentation membranes (GTAM) used for guided gone regeneration (GBR) procedures in edentulous ridge augmentation with or without implant placement. Cells were screened for mineralized nodule formation in vitro to mRNA analysis to demonstrate that they could form mineralized tissue. Production in interleukin-1 alpha (IL-1 alpha) interleukin-1 beta (IL-1 beta), interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) was evaluated by reverse transcribed-polymerase chain reaction (RT-PCR) of mRNA from rescued regenerative cells, human gingival fibroblasts and periodontal ligament (PDL) cells. Both the gingival fibroblast and PDL cells isolates produced all 4 cytokines. However, the cell isolates from the regenerative membranes had various profiles of cytokine expression. Most GTR cell isolates were positive for all 4 cytokines. IL-1 beta was produced by all 6 GTR cell isolates but was not detected at the same number of cycles of RT-PCR amplification in any of the 6 GBR cell isolates. IL-1 beta transcripts were also not observed in cells derived from a direct biopsy of GBR tissue. Cells were recovered from unexposed GBR membranes did not produce detectable amounts of IFN-gamma, whereas cells recovered from exposed GBR and all GTR membranes produced IFN-gamma. These findings indicate that cells from regenerative tissues express different cytokines and that exposure to the tissue to the oral cavity during healing may modulate this expression.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9089488</PMID><DateCompleted><Year>1997</Year><Month>06</Month><Day>10</Day></DateCompleted><DateRevised><Year>2008</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-3484</ISSN><JournalIssue CitedMedium="Print"><Volume>32</Volume><Issue>2</Issue><PubDate><Year>1997</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Journal of periodontal research</Title><ISOAbbreviation>J. Periodont. Res.</ISOAbbreviation></Journal><ArticleTitle>Cytokine production by cells adherent to regenerative membranes.</ArticleTitle><Pagination><MedlinePgn>215-24</MedlinePgn></Pagination><Abstract><AbstractText>Since cytokines play a critical role in tissue regeneration, we have assayed cytokine production by cells from tissue adherent to regenerative membranes. Cells were recovered from Gore-tex membranes in guided tissue regeneration (GTR) procedures to regenerate that attachment apparatus around teeth and from Gore-tex augmentation membranes (GTAM) used for guided gone regeneration (GBR) procedures in edentulous ridge augmentation with or without implant placement. Cells were screened for mineralized nodule formation in vitro to mRNA analysis to demonstrate that they could form mineralized tissue. Production in interleukin-1 alpha (IL-1 alpha) interleukin-1 beta (IL-1 beta), interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) was evaluated by reverse transcribed-polymerase chain reaction (RT-PCR) of mRNA from rescued regenerative cells, human gingival fibroblasts and periodontal ligament (PDL) cells. Both the gingival fibroblast and PDL cells isolates produced all 4 cytokines. However, the cell isolates from the regenerative membranes had various profiles of cytokine expression. Most GTR cell isolates were positive for all 4 cytokines. IL-1 beta was produced by all 6 GTR cell isolates but was not detected at the same number of cycles of RT-PCR amplification in any of the 6 GBR cell isolates. IL-1 beta transcripts were also not observed in cells derived from a direct biopsy of GBR tissue. Cells were recovered from unexposed GBR membranes did not produce detectable amounts of IFN-gamma, whereas cells recovered from exposed GBR and all GTR membranes produced IFN-gamma. These findings indicate that cells from regenerative tissues express different cytokines and that exposure to the tissue to the oral cavity during healing may modulate this expression.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wakabayashi</LastName><ForeName>R C</ForeName><Initials>RC</Initials><AffiliationInfo><Affiliation>Department of Stomatology, School of Dentistry, University of California, San Francisco 94143-0650, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Iha</LastName><ForeName>D K</ForeName><Initials>DK</Initials></Author><Author ValidYN="Y"><LastName>Niu</LastName><ForeName>J J</ForeName><Initials>JJ</Initials></Author><Author ValidYN="Y"><LastName>Johnson</LastName><ForeName>P W</ForeName><Initials>PW</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>1 RO 3 DE10783</GrantID><Acronym>DE</Acronym><Agency>NIDCR NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Periodontal Res</MedlineTA><NlmUniqueID>0055107</NlmUniqueID><ISSNLinking>0022-3484</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016207">Cytokines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015921">Dental Implants</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007375">Interleukin-1</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008567">Membranes, Artificial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>207137-56-2</RegistryNumber><NameOfSubstance UI="D015847">Interleukin-4</NameOfSubstance></Chemical><Chemical><RegistryNumber>82115-62-6</RegistryNumber><NameOfSubstance UI="D007371">Interferon-gamma</NameOfSubstance></Chemical><Chemical><RegistryNumber>9002-84-0</RegistryNumber><NameOfSubstance UI="D011138">Polytetrafluoroethylene</NameOfSubstance></Chemical></ChemicalList><CitationSubset>D</CitationSubset><CitationSubset>IM</CitationSubset><CitationSubset>S</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000540" MajorTopicYN="N">Alveolar Ridge Augmentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001861" MajorTopicYN="N">Bone Regeneration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002113" MajorTopicYN="N">Calcification, Physiologic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002448" MajorTopicYN="N">Cell Adhesion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002469" MajorTopicYN="N">Cell Separation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016207" MajorTopicYN="N">Cytokines</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015921" MajorTopicYN="N">Dental Implants</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005347" MajorTopicYN="N">Fibroblasts</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005881" MajorTopicYN="N">Gingiva</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016557" MajorTopicYN="Y">Guided Tissue Regeneration, Periodontal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007371" MajorTopicYN="N">Interferon-gamma</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007375" MajorTopicYN="N">Interleukin-1</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015847" MajorTopicYN="N">Interleukin-4</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007575" MajorTopicYN="N">Jaw, Edentulous</DescriptorName><QualifierName UI="Q000601" MajorTopicYN="N">surgery</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008567" MajorTopicYN="Y">Membranes, Artificial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010513" MajorTopicYN="N">Periodontal Ligament</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011138" MajorTopicYN="N">Polytetrafluoroethylene</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1997</Year><Month>2</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1997</Year><Month>2</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1997</Year><Month>2</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9089488</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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