Microbial analysis of bone collected during implant surgery: a clinical and laboratory study.
Identifieur interne : 003489 ( PubMed/Corpus ); précédent : 003488; suivant : 003490Microbial analysis of bone collected during implant surgery: a clinical and laboratory study.
Auteurs : M P Young ; D H Carter ; H. Worthington ; M. Korachi ; D B DruckerSource :
- Clinical oral implants research [ 0905-7161 ] ; 2001.
English descriptors
- KwdEn :
- MESH :
- isolation & purification : Bacteria, Anaerobic, Gram-Positive Cocci.
- microbiology : Alveolar Process, Jaw, Edentulous, Partially.
- Colony Count, Microbial, Dental Implantation, Endosseous, Female, Humans, Male, Suction.
Abstract
Dental implant surgery produces bone debris which can be used to correct bone defects in the "simultaneous-augmentation" technique. However, this debris is potentially contaminated with oral bacteria. Therefore, this study examined bone debris collected during dental implant surgery in order 1) to identify the microbial contaminants and 2) to compare the effects of two different aspiration protocols on the levels of microbial contamination. Twenty-four partially dentate patients were randomly allocated into two equal groups and underwent bone collection using the Frios Bone Collector during surgery to insert two endosseous dental implants. In group S (using a stringent aspiration protocol), bone collection occurred within the surgical site only. In group NS (utilizing a non-stringent aspiration protocol), bone collection and tissue fluid control was achieved using the same suction tip. Bone samples were immediately transported for microbial analysis. Colonial and microscopic morphology, gaseous requirements and identification kits were utilized for identification of the isolated microbes. Twenty-eight species were identified including a number associated with disease, in particular, Enterococcus faecalis and Staphylococcus epidermidis as well as the anaerobes Actinomyces odontolyticus, Eubacterium sp., Prevotella intermedia, Propionibacterium propionicum and Peptostreptococcus asaccharolyticus. In group S (stringent aspiration protocol), significantly fewer organisms were found than in group NS, the non-stringent aspiration protocol (P=0.001). Gram-positive cocci dominated the isolates from both groups. It is concluded that if bone debris is collected for implantation around dental implants, it should be collected with a stringent aspiration protocol (within the surgical site only) to minimize bacterial contaminants.
PubMed: 11251657
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pubmed:11251657Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Microbial analysis of bone collected during implant surgery: a clinical and laboratory study.</title><author><name sortKey="Young, M P" sort="Young, M P" uniqKey="Young M" first="M P" last="Young">M P Young</name><affiliation><nlm:affiliation>Department of Dental Surgery & Medicine, Turner Dental School, University of Manchester, Manchester, United Kingdom. David.Drucker@man.ac.uk</nlm:affiliation></affiliation></author><author><name sortKey="Carter, D H" sort="Carter, D H" uniqKey="Carter D" first="D H" last="Carter">D H Carter</name></author><author><name sortKey="Worthington, H" sort="Worthington, H" uniqKey="Worthington H" first="H" last="Worthington">H. Worthington</name></author><author><name sortKey="Korachi, M" sort="Korachi, M" uniqKey="Korachi M" first="M" last="Korachi">M. Korachi</name></author><author><name sortKey="Drucker, D B" sort="Drucker, D B" uniqKey="Drucker D" first="D B" last="Drucker">D B Drucker</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11251657</idno><idno type="pmid">11251657</idno><idno type="wicri:Area/PubMed/Corpus">003489</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003489</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Microbial analysis of bone collected during implant surgery: a clinical and laboratory study.</title><author><name sortKey="Young, M P" sort="Young, M P" uniqKey="Young M" first="M P" last="Young">M P Young</name><affiliation><nlm:affiliation>Department of Dental Surgery & Medicine, Turner Dental School, University of Manchester, Manchester, United Kingdom. David.Drucker@man.ac.uk</nlm:affiliation></affiliation></author><author><name sortKey="Carter, D H" sort="Carter, D H" uniqKey="Carter D" first="D H" last="Carter">D H Carter</name></author><author><name sortKey="Worthington, H" sort="Worthington, H" uniqKey="Worthington H" first="H" last="Worthington">H. Worthington</name></author><author><name sortKey="Korachi, M" sort="Korachi, M" uniqKey="Korachi M" first="M" last="Korachi">M. Korachi</name></author><author><name sortKey="Drucker, D B" sort="Drucker, D B" uniqKey="Drucker D" first="D B" last="Drucker">D B Drucker</name></author></analytic><series><title level="j">Clinical oral implants research</title><idno type="ISSN">0905-7161</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alveolar Process (microbiology)</term><term>Bacteria, Anaerobic (isolation & purification)</term><term>Colony Count, Microbial</term><term>Dental Implantation, Endosseous</term><term>Female</term><term>Gram-Positive Cocci (isolation & purification)</term><term>Humans</term><term>Jaw, Edentulous, Partially (microbiology)</term><term>Male</term><term>Suction</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacteria, Anaerobic</term><term>Gram-Positive Cocci</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Alveolar Process</term><term>Jaw, Edentulous, Partially</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Colony Count, Microbial</term><term>Dental Implantation, Endosseous</term><term>Female</term><term>Humans</term><term>Male</term><term>Suction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Dental implant surgery produces bone debris which can be used to correct bone defects in the "simultaneous-augmentation" technique. However, this debris is potentially contaminated with oral bacteria. Therefore, this study examined bone debris collected during dental implant surgery in order 1) to identify the microbial contaminants and 2) to compare the effects of two different aspiration protocols on the levels of microbial contamination. Twenty-four partially dentate patients were randomly allocated into two equal groups and underwent bone collection using the Frios Bone Collector during surgery to insert two endosseous dental implants. In group S (using a stringent aspiration protocol), bone collection occurred within the surgical site only. In group NS (utilizing a non-stringent aspiration protocol), bone collection and tissue fluid control was achieved using the same suction tip. Bone samples were immediately transported for microbial analysis. Colonial and microscopic morphology, gaseous requirements and identification kits were utilized for identification of the isolated microbes. Twenty-eight species were identified including a number associated with disease, in particular, Enterococcus faecalis and Staphylococcus epidermidis as well as the anaerobes Actinomyces odontolyticus, Eubacterium sp., Prevotella intermedia, Propionibacterium propionicum and Peptostreptococcus asaccharolyticus. In group S (stringent aspiration protocol), significantly fewer organisms were found than in group NS, the non-stringent aspiration protocol (P=0.001). Gram-positive cocci dominated the isolates from both groups. It is concluded that if bone debris is collected for implantation around dental implants, it should be collected with a stringent aspiration protocol (within the surgical site only) to minimize bacterial contaminants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11251657</PMID><DateCompleted><Year>2001</Year><Month>12</Month><Day>04</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0905-7161</ISSN><JournalIssue CitedMedium="Print"><Volume>12</Volume><Issue>2</Issue><PubDate><Year>2001</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Clinical oral implants research</Title><ISOAbbreviation>Clin Oral Implants Res</ISOAbbreviation></Journal><ArticleTitle>Microbial analysis of bone collected during implant surgery: a clinical and laboratory study.</ArticleTitle><Pagination><MedlinePgn>95-103</MedlinePgn></Pagination><Abstract><AbstractText>Dental implant surgery produces bone debris which can be used to correct bone defects in the "simultaneous-augmentation" technique. However, this debris is potentially contaminated with oral bacteria. Therefore, this study examined bone debris collected during dental implant surgery in order 1) to identify the microbial contaminants and 2) to compare the effects of two different aspiration protocols on the levels of microbial contamination. Twenty-four partially dentate patients were randomly allocated into two equal groups and underwent bone collection using the Frios Bone Collector during surgery to insert two endosseous dental implants. In group S (using a stringent aspiration protocol), bone collection occurred within the surgical site only. In group NS (utilizing a non-stringent aspiration protocol), bone collection and tissue fluid control was achieved using the same suction tip. Bone samples were immediately transported for microbial analysis. Colonial and microscopic morphology, gaseous requirements and identification kits were utilized for identification of the isolated microbes. Twenty-eight species were identified including a number associated with disease, in particular, Enterococcus faecalis and Staphylococcus epidermidis as well as the anaerobes Actinomyces odontolyticus, Eubacterium sp., Prevotella intermedia, Propionibacterium propionicum and Peptostreptococcus asaccharolyticus. In group S (stringent aspiration protocol), significantly fewer organisms were found than in group NS, the non-stringent aspiration protocol (P=0.001). Gram-positive cocci dominated the isolates from both groups. It is concluded that if bone debris is collected for implantation around dental implants, it should be collected with a stringent aspiration protocol (within the surgical site only) to minimize bacterial contaminants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Young</LastName><ForeName>M P</ForeName><Initials>MP</Initials><AffiliationInfo><Affiliation>Department of Dental Surgery & Medicine, Turner Dental School, University of Manchester, Manchester, United Kingdom. David.Drucker@man.ac.uk</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Carter</LastName><ForeName>D H</ForeName><Initials>DH</Initials></Author><Author ValidYN="Y"><LastName>Worthington</LastName><ForeName>H</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Korachi</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Drucker</LastName><ForeName>D B</ForeName><Initials>DB</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016430">Clinical Trial</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D016449">Randomized Controlled Trial</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Denmark</Country><MedlineTA>Clin Oral Implants Res</MedlineTA><NlmUniqueID>9105713</NlmUniqueID><ISSNLinking>0905-7161</ISSNLinking></MedlineJournalInfo><CitationSubset>D</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000539" MajorTopicYN="N">Alveolar Process</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001421" MajorTopicYN="N">Bacteria, Anaerobic</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015169" MajorTopicYN="N">Colony Count, Microbial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003758" MajorTopicYN="Y">Dental Implantation, Endosseous</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006095" MajorTopicYN="N">Gram-Positive Cocci</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007576" MajorTopicYN="N">Jaw, Edentulous, Partially</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013396" MajorTopicYN="N">Suction</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2001</Year><Month>3</Month><Day>17</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2002</Year><Month>1</Month><Day>5</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2001</Year><Month>3</Month><Day>17</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11251657</ArticleId><ArticleId IdType="pii">clr120201</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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