The mandibular ridge oral mucosa model of stromal influences on the endothelial tip cells: an immunohistochemical and TEM study.
Identifieur interne : 000D58 ( PubMed/Corpus ); précédent : 000D57; suivant : 000D59The mandibular ridge oral mucosa model of stromal influences on the endothelial tip cells: an immunohistochemical and TEM study.
Auteurs : Mugurel Constantin Rusu ; Andreea Cristiana Didilescu ; Ruxandra St Nescu ; Florinel Pop ; Valentina Mariana M Noiu ; Adelina Maria Jianu ; Marek VâlcuSource :
- Anatomical record (Hoboken, N.J. : 2007) [ 1932-8494 ] ; 2013.
English descriptors
- KwdEn :
- Adult, Biomarkers (analysis), Case-Control Studies, Dental Implantation, Endothelial Cells (chemistry), Endothelial Cells (ultrastructure), Female, Humans, Immunohistochemistry, Jaw, Edentulous (metabolism), Jaw, Edentulous (pathology), Jaw, Edentulous (surgery), Male, Mandible (blood supply), Mandible (chemistry), Mandible (ultrastructure), Microscopy, Electron, Transmission, Mouth Mucosa (blood supply), Mouth Mucosa (chemistry), Mouth Mucosa (ultrastructure), Neovascularization, Physiologic, Phenotype, Stromal Cells (chemistry), Stromal Cells (ultrastructure), Wound Healing.
- MESH :
- chemical , analysis : Biomarkers.
- blood supply : Mandible, Mouth Mucosa.
- chemistry : Endothelial Cells, Mandible, Mouth Mucosa, Stromal Cells.
- metabolism : Jaw, Edentulous.
- pathology : Jaw, Edentulous.
- surgery : Jaw, Edentulous.
- ultrastructure : Endothelial Cells, Mandible, Mouth Mucosa, Stromal Cells.
- Adult, Case-Control Studies, Dental Implantation, Female, Humans, Immunohistochemistry, Male, Microscopy, Electron, Transmission, Neovascularization, Physiologic, Phenotype, Wound Healing.
Abstract
This study aimed to evaluate by immunohistochemistry and transmission electron microscopy (TEM) the morphological features of the oral mucosa endothelial tip cells (ETCs) and to determine the immune and ultrastructural patterns of the stromal nonimmune cells which could influence healing processes. Immune labeling was performed on bioptic samples obtained from six edentulous patients undergoing surgery for dental implants placement; three normal samples were collected from patients prior to the extraction of the third mandibular molar. The antibodies were tested for CD34, CD117(c-kit), platelet derived growth factor receptor-alpha (PDGFR-α), Mast Cell Tryptase, CD44, vimentin, CD45, CD105, alpha-smooth muscle actin, FGF2, Ki67. In light microscopy, while stromal cells (StrCs) of the reparatory and normal oral mucosa, with a fibroblastic appearance, were found positive for a CD34/CD44/CD45/CD105/PDGFR-α/vimentin immune phenotype, the CD117/c-kit labeling led to a positive stromal reaction only in the reparatory mucosa. In TEM, non-immune StrCs presenting particular ultrastructural features were identified as circulating fibrocytes (CFCs). Within the lamina propria CFCs were in close contact with ETCs. Long processes of the ETCs were moniliform, and hook-like collaterals were arising from the dilated segments, suggestive for a different stage migration. Maintenance and healing of oral mucosa are so supported by extensive processes of angiogenesis, guided by ETCs that, in turn, are influenced by the CFCs that populate the stromal compartment both in normal and reparatory states. Therefore, CFCs could be targeted by specific therapies, with pro- or anti-angiogenic purposes.
DOI: 10.1002/ar.22630
PubMed: 23192856
Links to Exploration step
pubmed:23192856Le document en format XML
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uniqKey="Pop F" first="Florinel" last="Pop">Florinel Pop</name></author><author><name sortKey="M Noiu, Valentina Mariana" sort="M Noiu, Valentina Mariana" uniqKey="M Noiu V" first="Valentina Mariana" last="M Noiu">Valentina Mariana M Noiu</name></author><author><name sortKey="Jianu, Adelina Maria" sort="Jianu, Adelina Maria" uniqKey="Jianu A" first="Adelina Maria" last="Jianu">Adelina Maria Jianu</name></author><author><name sortKey="Valcu, Marek" sort="Valcu, Marek" uniqKey="Valcu M" first="Marek" last="Vâlcu">Marek Vâlcu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23192856</idno><idno type="pmid">23192856</idno><idno type="doi">10.1002/ar.22630</idno><idno type="wicri:Area/PubMed/Corpus">000D58</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000D58</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The mandibular ridge oral mucosa model of stromal influences on the endothelial tip cells: an immunohistochemical and TEM study.</title><author><name sortKey="Rusu, Mugurel Constantin" sort="Rusu, Mugurel Constantin" uniqKey="Rusu M" first="Mugurel Constantin" last="Rusu">Mugurel Constantin Rusu</name><affiliation><nlm:affiliation>Division of Anatomy, Faculty of Dental Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. anatomon@gmail.com</nlm:affiliation></affiliation></author><author><name sortKey="Didilescu, Andreea Cristiana" sort="Didilescu, Andreea Cristiana" uniqKey="Didilescu A" first="Andreea Cristiana" last="Didilescu">Andreea Cristiana Didilescu</name></author><author><name sortKey="St Nescu, Ruxandra" sort="St Nescu, Ruxandra" uniqKey="St Nescu R" first="Ruxandra" last="St Nescu">Ruxandra St Nescu</name></author><author><name sortKey="Pop, Florinel" sort="Pop, Florinel" uniqKey="Pop F" first="Florinel" last="Pop">Florinel Pop</name></author><author><name sortKey="M Noiu, Valentina Mariana" sort="M Noiu, Valentina Mariana" uniqKey="M Noiu V" first="Valentina Mariana" last="M Noiu">Valentina Mariana M Noiu</name></author><author><name sortKey="Jianu, Adelina Maria" sort="Jianu, Adelina Maria" uniqKey="Jianu A" first="Adelina Maria" last="Jianu">Adelina Maria Jianu</name></author><author><name sortKey="Valcu, Marek" sort="Valcu, Marek" uniqKey="Valcu M" first="Marek" last="Vâlcu">Marek Vâlcu</name></author></analytic><series><title level="j">Anatomical record (Hoboken, N.J. : 2007)</title><idno type="eISSN">1932-8494</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Biomarkers (analysis)</term><term>Case-Control Studies</term><term>Dental Implantation</term><term>Endothelial Cells (chemistry)</term><term>Endothelial Cells (ultrastructure)</term><term>Female</term><term>Humans</term><term>Immunohistochemistry</term><term>Jaw, Edentulous (metabolism)</term><term>Jaw, Edentulous (pathology)</term><term>Jaw, Edentulous (surgery)</term><term>Male</term><term>Mandible (blood supply)</term><term>Mandible (chemistry)</term><term>Mandible (ultrastructure)</term><term>Microscopy, Electron, Transmission</term><term>Mouth Mucosa (blood supply)</term><term>Mouth Mucosa (chemistry)</term><term>Mouth Mucosa (ultrastructure)</term><term>Neovascularization, Physiologic</term><term>Phenotype</term><term>Stromal Cells (chemistry)</term><term>Stromal Cells (ultrastructure)</term><term>Wound Healing</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Biomarkers</term></keywords><keywords scheme="MESH" qualifier="blood supply" xml:lang="en"><term>Mandible</term><term>Mouth Mucosa</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Endothelial Cells</term><term>Mandible</term><term>Mouth Mucosa</term><term>Stromal Cells</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Jaw, Edentulous</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Jaw, Edentulous</term></keywords><keywords scheme="MESH" qualifier="surgery" xml:lang="en"><term>Jaw, Edentulous</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Endothelial Cells</term><term>Mandible</term><term>Mouth Mucosa</term><term>Stromal Cells</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Case-Control Studies</term><term>Dental Implantation</term><term>Female</term><term>Humans</term><term>Immunohistochemistry</term><term>Male</term><term>Microscopy, Electron, Transmission</term><term>Neovascularization, Physiologic</term><term>Phenotype</term><term>Wound Healing</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study aimed to evaluate by immunohistochemistry and transmission electron microscopy (TEM) the morphological features of the oral mucosa endothelial tip cells (ETCs) and to determine the immune and ultrastructural patterns of the stromal nonimmune cells which could influence healing processes. Immune labeling was performed on bioptic samples obtained from six edentulous patients undergoing surgery for dental implants placement; three normal samples were collected from patients prior to the extraction of the third mandibular molar. The antibodies were tested for CD34, CD117(c-kit), platelet derived growth factor receptor-alpha (PDGFR-α), Mast Cell Tryptase, CD44, vimentin, CD45, CD105, alpha-smooth muscle actin, FGF2, Ki67. In light microscopy, while stromal cells (StrCs) of the reparatory and normal oral mucosa, with a fibroblastic appearance, were found positive for a CD34/CD44/CD45/CD105/PDGFR-α/vimentin immune phenotype, the CD117/c-kit labeling led to a positive stromal reaction only in the reparatory mucosa. In TEM, non-immune StrCs presenting particular ultrastructural features were identified as circulating fibrocytes (CFCs). Within the lamina propria CFCs were in close contact with ETCs. Long processes of the ETCs were moniliform, and hook-like collaterals were arising from the dilated segments, suggestive for a different stage migration. Maintenance and healing of oral mucosa are so supported by extensive processes of angiogenesis, guided by ETCs that, in turn, are influenced by the CFCs that populate the stromal compartment both in normal and reparatory states. Therefore, CFCs could be targeted by specific therapies, with pro- or anti-angiogenic purposes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23192856</PMID><DateCompleted><Year>2013</Year><Month>07</Month><Day>09</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1932-8494</ISSN><JournalIssue CitedMedium="Internet"><Volume>296</Volume><Issue>2</Issue><PubDate><Year>2013</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Anatomical record (Hoboken, N.J. : 2007)</Title><ISOAbbreviation>Anat Rec (Hoboken)</ISOAbbreviation></Journal><ArticleTitle>The mandibular ridge oral mucosa model of stromal influences on the endothelial tip cells: an immunohistochemical and TEM study.</ArticleTitle><Pagination><MedlinePgn>350-63</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/ar.22630</ELocationID><Abstract><AbstractText>This study aimed to evaluate by immunohistochemistry and transmission electron microscopy (TEM) the morphological features of the oral mucosa endothelial tip cells (ETCs) and to determine the immune and ultrastructural patterns of the stromal nonimmune cells which could influence healing processes. Immune labeling was performed on bioptic samples obtained from six edentulous patients undergoing surgery for dental implants placement; three normal samples were collected from patients prior to the extraction of the third mandibular molar. The antibodies were tested for CD34, CD117(c-kit), platelet derived growth factor receptor-alpha (PDGFR-α), Mast Cell Tryptase, CD44, vimentin, CD45, CD105, alpha-smooth muscle actin, FGF2, Ki67. In light microscopy, while stromal cells (StrCs) of the reparatory and normal oral mucosa, with a fibroblastic appearance, were found positive for a CD34/CD44/CD45/CD105/PDGFR-α/vimentin immune phenotype, the CD117/c-kit labeling led to a positive stromal reaction only in the reparatory mucosa. In TEM, non-immune StrCs presenting particular ultrastructural features were identified as circulating fibrocytes (CFCs). Within the lamina propria CFCs were in close contact with ETCs. Long processes of the ETCs were moniliform, and hook-like collaterals were arising from the dilated segments, suggestive for a different stage migration. Maintenance and healing of oral mucosa are so supported by extensive processes of angiogenesis, guided by ETCs that, in turn, are influenced by the CFCs that populate the stromal compartment both in normal and reparatory states. Therefore, CFCs could be targeted by specific therapies, with pro- or anti-angiogenic purposes.</AbstractText><CopyrightInformation>Copyright © 2012 Wiley Periodicals, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rusu</LastName><ForeName>Mugurel Constantin</ForeName><Initials>MC</Initials><AffiliationInfo><Affiliation>Division of Anatomy, Faculty of Dental Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. anatomon@gmail.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Didilescu</LastName><ForeName>Andreea Cristiana</ForeName><Initials>AC</Initials></Author><Author ValidYN="Y"><LastName>Stănescu</LastName><ForeName>Ruxandra</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Pop</LastName><ForeName>Florinel</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Mănoiu</LastName><ForeName>Valentina Mariana</ForeName><Initials>VM</Initials></Author><Author ValidYN="Y"><LastName>Jianu</LastName><ForeName>Adelina Maria</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Vâlcu</LastName><ForeName>Marek</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>11</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Anat Rec (Hoboken)</MedlineTA><NlmUniqueID>101292775</NlmUniqueID><ISSNLinking>1932-8486</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015415">Biomarkers</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015415" MajorTopicYN="N">Biomarkers</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016022" MajorTopicYN="N">Case-Control Studies</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003757" MajorTopicYN="N">Dental Implantation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042783" MajorTopicYN="Y">Endothelial Cells</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007150" MajorTopicYN="Y">Immunohistochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007575" MajorTopicYN="Y">Jaw, Edentulous</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000473" MajorTopicYN="N">pathology</QualifierName><QualifierName UI="Q000601" MajorTopicYN="N">surgery</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008334" MajorTopicYN="Y">Mandible</DescriptorName><QualifierName UI="Q000098" MajorTopicYN="N">blood supply</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046529" MajorTopicYN="Y">Microscopy, Electron, Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009061" MajorTopicYN="Y">Mouth Mucosa</DescriptorName><QualifierName UI="Q000098" MajorTopicYN="N">blood supply</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018919" MajorTopicYN="N">Neovascularization, Physiologic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017154" MajorTopicYN="Y">Stromal Cells</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014945" MajorTopicYN="N">Wound Healing</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>05</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>08</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>10</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>11</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>11</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23192856</ArticleId><ArticleId IdType="doi">10.1002/ar.22630</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour 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