Analysis of early biofilm formation on oral implants in man.
Identifieur interne : 001E68 ( PubMed/Checkpoint ); précédent : 001E67; suivant : 001E69Analysis of early biofilm formation on oral implants in man.
Auteurs : W. Heuer [Allemagne] ; C. Elter ; A. Demling ; A. Neumann ; S. Suerbaum ; M. Hannig ; T. Heidenblut ; F W Bach ; M. Stiesch-ScholzSource :
- Journal of oral rehabilitation [ 0305-182X ] ; 2007.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Biofilms (croissance et développement), Exsudat gingival (microbiologie), Femelle, Haemophilus (isolement et purification), Humains, Implants dentaires (microbiologie), Mâchoire partiellement édentée (microbiologie), Mâle, Piliers dentaires (microbiologie), Porphyromonas gingivalis (isolement et purification), Réaction de polymérisation en chaîne (), Sujet âgé.
- MESH :
- croissance et développement : Biofilms.
- isolement et purification : Haemophilus, Porphyromonas gingivalis.
- microbiologie : Exsudat gingival, Implants dentaires, Mâchoire partiellement édentée, Piliers dentaires.
- Adolescent, Adulte, Adulte d'âge moyen, Femelle, Humains, Mâle, Réaction de polymérisation en chaîne, Sujet âgé.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Biofilms (growth & development), Dental Abutments (microbiology), Dental Implants (microbiology), Female, Gingival Crevicular Fluid (microbiology), Haemophilus (isolation & purification), Humans, Jaw, Edentulous, Partially (microbiology), Male, Middle Aged, Polymerase Chain Reaction (methods), Porphyromonas gingivalis (isolation & purification).
- MESH :
- chemical , microbiology : Dental Implants.
- growth & development : Biofilms.
- isolation & purification : Haemophilus, Porphyromonas gingivalis.
- methods : Polymerase Chain Reaction.
- microbiology : Dental Abutments, Gingival Crevicular Fluid, Jaw, Edentulous, Partially.
- Adolescent, Adult, Aged, Female, Humans, Male, Middle Aged.
Abstract
Biofilm formation on oral implants can cause inflammation of peri-implant tissues, which endangers the long-term success of osseointegrated implants. It has been reported previously that implants revealing signs of peri-implantitis contain subgingival microbiota similar to those of natural teeth with periodontitis. The purpose of the first part of this study was an atraumatic, quantitative investigation of biofilm formation on oral implant abutments; the objective of the second part was to investigate whether Haemophilus actinomycetemcomitans and Porphyromonas gingivalis were present in the crevicular fluid around oral implants. Biofilm formation on 14 healing abutments, inserted for 14 days in 10 patients, was analysed quantitatively by use of secondary-electron and Rutherford-backscattering-detection methods. A 16S rRNA-based polymerase chain reaction detection method was used to detect the presence of H. actinomycetemcomitans and P. gingivalis in the crevicular fluid. For this investigation, samples of sulcus fluid were collected with sterile paper points at four measurement points per abutment. The difference between biofilm coverage of supragingival surfaces (17.5 +/- 18.3%) and subgingival surfaces (0.8 +/- 1.0%) was statistically significant (P < 0.05). By use of universal primers, bacteria were found in all the samples taken, although the two periodontal pathogens were not found in any of the samples. The absence of periodontal pathogens from the sulcus fluid during initial bacterial colonization, despite massive supragingival biofilm formation, substantiates the assumption that cellular adherence of peri-implant tissue by means of hemidesmosoma, actin filaments and microvilli reduces the risk of formation of anaerobic subgingival pockets.
DOI: 10.1111/j.1365-2842.2007.01725.x
PubMed: 17441878
Affiliations:
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Heuer</name><affiliation wicri:level="3"><nlm:affiliation>Department of Prosthetic Dentistry, Hannover Medical School, Hannover, Germany. heuer.wieland@mh-hannover.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Prosthetic Dentistry, Hannover Medical School, Hannover</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author><author><name sortKey="Elter, C" sort="Elter, C" uniqKey="Elter C" first="C" last="Elter">C. Elter</name></author><author><name sortKey="Demling, A" sort="Demling, A" uniqKey="Demling A" first="A" last="Demling">A. Demling</name></author><author><name sortKey="Neumann, A" sort="Neumann, A" uniqKey="Neumann A" first="A" last="Neumann">A. Neumann</name></author><author><name sortKey="Suerbaum, S" sort="Suerbaum, S" uniqKey="Suerbaum S" first="S" last="Suerbaum">S. Suerbaum</name></author><author><name sortKey="Hannig, M" sort="Hannig, M" uniqKey="Hannig M" first="M" last="Hannig">M. Hannig</name></author><author><name sortKey="Heidenblut, T" sort="Heidenblut, T" uniqKey="Heidenblut T" first="T" last="Heidenblut">T. Heidenblut</name></author><author><name sortKey="Bach, F W" sort="Bach, F W" uniqKey="Bach F" first="F W" last="Bach">F W Bach</name></author><author><name sortKey="Stiesch Scholz, M" sort="Stiesch Scholz, M" uniqKey="Stiesch Scholz M" first="M" last="Stiesch-Scholz">M. Stiesch-Scholz</name></author></analytic><series><title level="j">Journal of oral rehabilitation</title><idno type="ISSN">0305-182X</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Biofilms (growth & development)</term><term>Dental Abutments (microbiology)</term><term>Dental Implants (microbiology)</term><term>Female</term><term>Gingival Crevicular Fluid (microbiology)</term><term>Haemophilus (isolation & purification)</term><term>Humans</term><term>Jaw, Edentulous, Partially (microbiology)</term><term>Male</term><term>Middle Aged</term><term>Polymerase Chain Reaction (methods)</term><term>Porphyromonas gingivalis (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Biofilms (croissance et développement)</term><term>Exsudat gingival (microbiologie)</term><term>Femelle</term><term>Haemophilus (isolement et purification)</term><term>Humains</term><term>Implants dentaires (microbiologie)</term><term>Mâchoire partiellement édentée (microbiologie)</term><term>Mâle</term><term>Piliers dentaires (microbiologie)</term><term>Porphyromonas gingivalis (isolement et purification)</term><term>Réaction de polymérisation en chaîne ()</term><term>Sujet âgé</term></keywords><keywords scheme="MESH" type="chemical" qualifier="microbiology" xml:lang="en"><term>Dental Implants</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Biofilms</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Biofilms</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Haemophilus</term><term>Porphyromonas gingivalis</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Haemophilus</term><term>Porphyromonas gingivalis</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Exsudat gingival</term><term>Implants dentaires</term><term>Mâchoire partiellement édentée</term><term>Piliers dentaires</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Dental Abutments</term><term>Gingival Crevicular Fluid</term><term>Jaw, Edentulous, Partially</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Réaction de polymérisation en chaîne</term><term>Sujet âgé</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Biofilm formation on oral implants can cause inflammation of peri-implant tissues, which endangers the long-term success of osseointegrated implants. It has been reported previously that implants revealing signs of peri-implantitis contain subgingival microbiota similar to those of natural teeth with periodontitis. The purpose of the first part of this study was an atraumatic, quantitative investigation of biofilm formation on oral implant abutments; the objective of the second part was to investigate whether Haemophilus actinomycetemcomitans and Porphyromonas gingivalis were present in the crevicular fluid around oral implants. Biofilm formation on 14 healing abutments, inserted for 14 days in 10 patients, was analysed quantitatively by use of secondary-electron and Rutherford-backscattering-detection methods. A 16S rRNA-based polymerase chain reaction detection method was used to detect the presence of H. actinomycetemcomitans and P. gingivalis in the crevicular fluid. For this investigation, samples of sulcus fluid were collected with sterile paper points at four measurement points per abutment. The difference between biofilm coverage of supragingival surfaces (17.5 +/- 18.3%) and subgingival surfaces (0.8 +/- 1.0%) was statistically significant (P < 0.05). By use of universal primers, bacteria were found in all the samples taken, although the two periodontal pathogens were not found in any of the samples. The absence of periodontal pathogens from the sulcus fluid during initial bacterial colonization, despite massive supragingival biofilm formation, substantiates the assumption that cellular adherence of peri-implant tissue by means of hemidesmosoma, actin filaments and microvilli reduces the risk of formation of anaerobic subgingival pockets.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17441878</PMID><DateCompleted><Year>2007</Year><Month>07</Month><Day>30</Day></DateCompleted><DateRevised><Year>2007</Year><Month>04</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0305-182X</ISSN><JournalIssue CitedMedium="Print"><Volume>34</Volume><Issue>5</Issue><PubDate><Year>2007</Year><Month>May</Month></PubDate></JournalIssue><Title>Journal of oral rehabilitation</Title><ISOAbbreviation>J Oral Rehabil</ISOAbbreviation></Journal><ArticleTitle>Analysis of early biofilm formation on oral implants in man.</ArticleTitle><Pagination><MedlinePgn>377-82</MedlinePgn></Pagination><Abstract><AbstractText>Biofilm formation on oral implants can cause inflammation of peri-implant tissues, which endangers the long-term success of osseointegrated implants. It has been reported previously that implants revealing signs of peri-implantitis contain subgingival microbiota similar to those of natural teeth with periodontitis. The purpose of the first part of this study was an atraumatic, quantitative investigation of biofilm formation on oral implant abutments; the objective of the second part was to investigate whether Haemophilus actinomycetemcomitans and Porphyromonas gingivalis were present in the crevicular fluid around oral implants. Biofilm formation on 14 healing abutments, inserted for 14 days in 10 patients, was analysed quantitatively by use of secondary-electron and Rutherford-backscattering-detection methods. A 16S rRNA-based polymerase chain reaction detection method was used to detect the presence of H. actinomycetemcomitans and P. gingivalis in the crevicular fluid. For this investigation, samples of sulcus fluid were collected with sterile paper points at four measurement points per abutment. The difference between biofilm coverage of supragingival surfaces (17.5 +/- 18.3%) and subgingival surfaces (0.8 +/- 1.0%) was statistically significant (P < 0.05). By use of universal primers, bacteria were found in all the samples taken, although the two periodontal pathogens were not found in any of the samples. The absence of periodontal pathogens from the sulcus fluid during initial bacterial colonization, despite massive supragingival biofilm formation, substantiates the assumption that cellular adherence of peri-implant tissue by means of hemidesmosoma, actin filaments and microvilli reduces the risk of formation of anaerobic subgingival pockets.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Heuer</LastName><ForeName>W</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Prosthetic Dentistry, Hannover Medical School, Hannover, Germany. heuer.wieland@mh-hannover.de</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Elter</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Demling</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Neumann</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Suerbaum</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Hannig</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Heidenblut</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Bach</LastName><ForeName>F W</ForeName><Initials>FW</Initials></Author><Author ValidYN="Y"><LastName>Stiesch-Scholz</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Oral Rehabil</MedlineTA><NlmUniqueID>0433604</NlmUniqueID><ISSNLinking>0305-182X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015921">Dental Implants</NameOfSubstance></Chemical></ChemicalList><CitationSubset>D</CitationSubset><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018441" MajorTopicYN="N">Biofilms</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000044" MajorTopicYN="N">Dental Abutments</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015921" MajorTopicYN="N">Dental Implants</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005883" MajorTopicYN="N">Gingival Crevicular Fluid</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006190" MajorTopicYN="N">Haemophilus</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007576" MajorTopicYN="N">Jaw, Edentulous, Partially</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016966" MajorTopicYN="N">Porphyromonas gingivalis</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>4</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>7</Month><Day>31</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>4</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17441878</ArticleId><ArticleId IdType="pii">JOR1725</ArticleId><ArticleId IdType="doi">10.1111/j.1365-2842.2007.01725.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Basse-Saxe</li></region><settlement><li>Hanovre</li></settlement></list><tree><noCountry><name sortKey="Bach, F W" sort="Bach, F W" uniqKey="Bach F" first="F W" last="Bach">F W Bach</name><name sortKey="Demling, A" sort="Demling, A" uniqKey="Demling A" first="A" last="Demling">A. Demling</name><name sortKey="Elter, C" sort="Elter, C" uniqKey="Elter C" first="C" last="Elter">C. Elter</name><name sortKey="Hannig, M" sort="Hannig, M" uniqKey="Hannig M" first="M" last="Hannig">M. Hannig</name><name sortKey="Heidenblut, T" sort="Heidenblut, T" uniqKey="Heidenblut T" first="T" last="Heidenblut">T. Heidenblut</name><name sortKey="Neumann, A" sort="Neumann, A" uniqKey="Neumann A" first="A" last="Neumann">A. Neumann</name><name sortKey="Stiesch Scholz, M" sort="Stiesch Scholz, M" uniqKey="Stiesch Scholz M" first="M" last="Stiesch-Scholz">M. Stiesch-Scholz</name><name sortKey="Suerbaum, S" sort="Suerbaum, S" uniqKey="Suerbaum S" first="S" last="Suerbaum">S. Suerbaum</name></noCountry><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="Heuer, W" sort="Heuer, W" uniqKey="Heuer W" first="W" last="Heuer">W. Heuer</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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