Relationship between Oral Anaerobic Bacteria and Otitis Media with Effusion
Identifieur interne : 002E36 ( Pmc/Curation ); précédent : 002E35; suivant : 002E37Relationship between Oral Anaerobic Bacteria and Otitis Media with Effusion
Auteurs : Nursen Topcuoglu [Turquie] ; Fahriye Keskin [Turquie] ; Sevgi Ciftci [Turquie] ; Ceki Paltura [Turquie] ; Mehmet Kulekci [Turquie] ; Duran Ustek [Turquie] ; Guven Kulekci [Turquie]Source :
- International Journal of Medical Sciences [ 1449-1907 ] ; 2012.
Abstract
Url:
DOI: 10.7150/ijms.4382
PubMed: 22606045
PubMed Central: 3354330
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002E36
Links to Exploration step
PMC:3354330Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Relationship between Oral Anaerobic Bacteria and Otitis Media with Effusion</title>
<author><name sortKey="Topcuoglu, Nursen" sort="Topcuoglu, Nursen" uniqKey="Topcuoglu N" first="Nursen" last="Topcuoglu">Nursen Topcuoglu</name>
<affiliation wicri:level="1"><nlm:aff id="A1">1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul, Turkey;</nlm:aff>
<country xml:lang="fr">Turquie</country>
<wicri:regionArea>1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Keskin, Fahriye" sort="Keskin, Fahriye" uniqKey="Keskin F" first="Fahriye" last="Keskin">Fahriye Keskin</name>
<affiliation wicri:level="1"><nlm:aff id="A1">1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul, Turkey;</nlm:aff>
<country xml:lang="fr">Turquie</country>
<wicri:regionArea>1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ciftci, Sevgi" sort="Ciftci, Sevgi" uniqKey="Ciftci S" first="Sevgi" last="Ciftci">Sevgi Ciftci</name>
<affiliation wicri:level="1"><nlm:aff id="A1">1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul, Turkey;</nlm:aff>
<country xml:lang="fr">Turquie</country>
<wicri:regionArea>1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Paltura, Ceki" sort="Paltura, Ceki" uniqKey="Paltura C" first="Ceki" last="Paltura">Ceki Paltura</name>
<affiliation wicri:level="1"><nlm:aff id="A2">2. Department of Otorhinolaryngology, Head and Neck Surgery, Taksim Training and Research Hospital, Istanbul, Turkey;</nlm:aff>
<country xml:lang="fr">Turquie</country>
<wicri:regionArea>2. Department of Otorhinolaryngology, Head and Neck Surgery, Taksim Training and Research Hospital, Istanbul</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kulekci, Mehmet" sort="Kulekci, Mehmet" uniqKey="Kulekci M" first="Mehmet" last="Kulekci">Mehmet Kulekci</name>
<affiliation wicri:level="1"><nlm:aff id="A2">2. Department of Otorhinolaryngology, Head and Neck Surgery, Taksim Training and Research Hospital, Istanbul, Turkey;</nlm:aff>
<country xml:lang="fr">Turquie</country>
<wicri:regionArea>2. Department of Otorhinolaryngology, Head and Neck Surgery, Taksim Training and Research Hospital, Istanbul</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ustek, Duran" sort="Ustek, Duran" uniqKey="Ustek D" first="Duran" last="Ustek">Duran Ustek</name>
<affiliation wicri:level="1"><nlm:aff id="A3">3. Department of Genetics, Institute for Experimental Medicine, Istanbul Faculty of Medicine, Istanbul, Turkey.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Turquie</country>
<wicri:regionArea>3. Department of Genetics, Institute for Experimental Medicine, Istanbul Faculty of Medicine, Istanbul</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kulekci, Guven" sort="Kulekci, Guven" uniqKey="Kulekci G" first="Guven" last="Kulekci">Guven Kulekci</name>
<affiliation wicri:level="1"><nlm:aff id="A1">1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul, Turkey;</nlm:aff>
<country xml:lang="fr">Turquie</country>
<wicri:regionArea>1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">22606045</idno>
<idno type="pmc">3354330</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3354330</idno>
<idno type="RBID">PMC:3354330</idno>
<idno type="doi">10.7150/ijms.4382</idno>
<date when="2012">2012</date>
<idno type="wicri:Area/Pmc/Corpus">002E36</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002E36</idno>
<idno type="wicri:Area/Pmc/Curation">002E36</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002E36</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Relationship between Oral Anaerobic Bacteria and Otitis Media with Effusion</title>
<author><name sortKey="Topcuoglu, Nursen" sort="Topcuoglu, Nursen" uniqKey="Topcuoglu N" first="Nursen" last="Topcuoglu">Nursen Topcuoglu</name>
<affiliation wicri:level="1"><nlm:aff id="A1">1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul, Turkey;</nlm:aff>
<country xml:lang="fr">Turquie</country>
<wicri:regionArea>1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Keskin, Fahriye" sort="Keskin, Fahriye" uniqKey="Keskin F" first="Fahriye" last="Keskin">Fahriye Keskin</name>
<affiliation wicri:level="1"><nlm:aff id="A1">1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul, Turkey;</nlm:aff>
<country xml:lang="fr">Turquie</country>
<wicri:regionArea>1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ciftci, Sevgi" sort="Ciftci, Sevgi" uniqKey="Ciftci S" first="Sevgi" last="Ciftci">Sevgi Ciftci</name>
<affiliation wicri:level="1"><nlm:aff id="A1">1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul, Turkey;</nlm:aff>
<country xml:lang="fr">Turquie</country>
<wicri:regionArea>1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Paltura, Ceki" sort="Paltura, Ceki" uniqKey="Paltura C" first="Ceki" last="Paltura">Ceki Paltura</name>
<affiliation wicri:level="1"><nlm:aff id="A2">2. Department of Otorhinolaryngology, Head and Neck Surgery, Taksim Training and Research Hospital, Istanbul, Turkey;</nlm:aff>
<country xml:lang="fr">Turquie</country>
<wicri:regionArea>2. Department of Otorhinolaryngology, Head and Neck Surgery, Taksim Training and Research Hospital, Istanbul</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kulekci, Mehmet" sort="Kulekci, Mehmet" uniqKey="Kulekci M" first="Mehmet" last="Kulekci">Mehmet Kulekci</name>
<affiliation wicri:level="1"><nlm:aff id="A2">2. Department of Otorhinolaryngology, Head and Neck Surgery, Taksim Training and Research Hospital, Istanbul, Turkey;</nlm:aff>
<country xml:lang="fr">Turquie</country>
<wicri:regionArea>2. Department of Otorhinolaryngology, Head and Neck Surgery, Taksim Training and Research Hospital, Istanbul</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Ustek, Duran" sort="Ustek, Duran" uniqKey="Ustek D" first="Duran" last="Ustek">Duran Ustek</name>
<affiliation wicri:level="1"><nlm:aff id="A3">3. Department of Genetics, Institute for Experimental Medicine, Istanbul Faculty of Medicine, Istanbul, Turkey.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Turquie</country>
<wicri:regionArea>3. Department of Genetics, Institute for Experimental Medicine, Istanbul Faculty of Medicine, Istanbul</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kulekci, Guven" sort="Kulekci, Guven" uniqKey="Kulekci G" first="Guven" last="Kulekci">Guven Kulekci</name>
<affiliation wicri:level="1"><nlm:aff id="A1">1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul, Turkey;</nlm:aff>
<country xml:lang="fr">Turquie</country>
<wicri:regionArea>1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">International Journal of Medical Sciences</title>
<idno type="eISSN">1449-1907</idno>
<imprint><date when="2012">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p><bold>Objective:</bold>
In this study hypothesing the translocation of oral bacteria from oropharynx into the middle ear cavity may be involved in the pathogenesis of otitis media with effusion (OME), we aimed to investigate the presence and similarity of <italic>Fusobacterium nucleatum</italic>
and <italic>Treponema denticola</italic>
in saliva, nasopharyngeal secretion and the middle ear effusion samples from the children with OME.</p>
<p><bold>Methods:</bold>
Totally 20 children with OME undergoing myringotomy and ventilation tube placement were attended. Stimulated saliva samples were collected after otorhinolaryngological and oral examinations were done. The middle ear effusion and nasopharyngeal secretions were collected during the operations. The presence of <italic>F. nucleatum</italic>
and <italic>T. denticola</italic>
were detected using 16SrRNA-based PCR. The clonal similarities of the bacteria were detected in the samples which the same bacteria had been detected in each samples of the same child. After DNA sequencing, clonal similarity was determined by 16SrRNA gene clone library analysis. The sequences from each clone were compared with similar sequences of reference organisms by FASTA search.</p>
<p><bold>Results:</bold>
<italic> T. denticola</italic>
was detected only in four (20%) saliva and in one (5%) nasopharyngeal sample. <italic>F. nucleatum</italic>
was detected in 11 (55%) saliva, eight (40%) nasopharyngeal and six (30%) middle ear effusion samples. Sequences from <italic>F.nucleatum</italic>
clones derived from three different anatomic sites within patients were similar in 33% of OME patients, indicating their genetic relatedness.</p>
<p><bold>Conclusions:</bold>
Bacteria involved in this process most likely originate from the oropharynx since they show a close genetic relatedness with their oropharyngeal counterparts.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Bluestone, Cd" uniqKey="Bluestone C">CD Bluestone</name>
</author>
<author><name sortKey="Klein, Jo" uniqKey="Klein J">JO Klein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bennett, Ke" uniqKey="Bennett K">KE Bennett</name>
</author>
<author><name sortKey="Haggard, Mp" uniqKey="Haggard M">MP Haggard</name>
</author>
<author><name sortKey="Silva, Pa" uniqKey="Silva P">PA Silva</name>
</author>
<author><name sortKey="Stewart, Ia" uniqKey="Stewart I">IA Stewart</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hotomi, M" uniqKey="Hotomi M">M Hotomi</name>
</author>
<author><name sortKey="Tabata, T" uniqKey="Tabata T">T Tabata</name>
</author>
<author><name sortKey="Kakiuchi, H" uniqKey="Kakiuchi H">H Kakiuchi</name>
</author>
<author><name sortKey="Kunimoto, M" uniqKey="Kunimoto M">M Kunimoto</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Post, Jc" uniqKey="Post J">JC Post</name>
</author>
<author><name sortKey="Preston, Ra" uniqKey="Preston R">RA Preston</name>
</author>
<author><name sortKey="Aul, Jj" uniqKey="Aul J">JJ Aul</name>
</author>
<author><name sortKey="Larkins Pettigrew, M" uniqKey="Larkins Pettigrew M">M Larkins-Pettigrew</name>
</author>
<author><name sortKey="Rydquist White, J" uniqKey="Rydquist White J">J Rydquist-White</name>
</author>
<author><name sortKey="Anderson, Kw" uniqKey="Anderson K">KW Anderson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rayner, Mg" uniqKey="Rayner M">MG Rayner</name>
</author>
<author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name>
</author>
<author><name sortKey="Gorry, Mc" uniqKey="Gorry M">MC Gorry</name>
</author>
<author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name>
</author>
<author><name sortKey="Post, Jc" uniqKey="Post J">JC Post</name>
</author>
<author><name sortKey="Ehrlich, Gd" uniqKey="Ehrlich G">GD Ehrlich</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pereira, M B" uniqKey="Pereira M">M.B Pereira</name>
</author>
<author><name sortKey="Pereira, Mr" uniqKey="Pereira M">MR Pereira</name>
</author>
<author><name sortKey="Cantarelli, V" uniqKey="Cantarelli V">V Cantarelli</name>
</author>
<author><name sortKey="Costa, Ss" uniqKey="Costa S">SS Costa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kulekci, G" uniqKey="Kulekci G">G Külekci</name>
</author>
<author><name sortKey="Ciftci, S" uniqKey="Ciftci S">S Ciftci</name>
</author>
<author><name sortKey="Keskin, F" uniqKey="Keskin F">F Keskin</name>
</author>
<author><name sortKey="Kilic, Ao" uniqKey="Kilic A">AO Kilic</name>
</author>
<author><name sortKey="Turkoglu, S" uniqKey="Turkoglu S">S Türkoglu</name>
</author>
<author><name sortKey="Badur, S" uniqKey="Badur S">S Badur</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kulekci, G" uniqKey="Kulekci G">G Külekci</name>
</author>
<author><name sortKey="Leblebicioglu, B" uniqKey="Leblebicioglu B">B Leblebicioglu</name>
</author>
<author><name sortKey="Keskin, F" uniqKey="Keskin F">F Keskin</name>
</author>
<author><name sortKey="Ciftci, S" uniqKey="Ciftci S">S Ciftci</name>
</author>
<author><name sortKey="Badur, S" uniqKey="Badur S">S Badur</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Giebink, Gs" uniqKey="Giebink G">GS Giebink</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kononen, E" uniqKey="Kononen E">E Könönen</name>
</author>
<author><name sortKey="Syrjanen, R" uniqKey="Syrjanen R">R Syrjanen</name>
</author>
<author><name sortKey="Takala, A" uniqKey="Takala A">A Takala</name>
</author>
<author><name sortKey="Jousimies Somer, H" uniqKey="Jousimies Somer H">H Jousimies-Somer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kononen, E" uniqKey="Kononen E">E Könönen</name>
</author>
<author><name sortKey="Kanervo, A" uniqKey="Kanervo A">A Kanervo</name>
</author>
<author><name sortKey="Bryk, A" uniqKey="Bryk A">A Bryk</name>
</author>
<author><name sortKey="Takala, A" uniqKey="Takala A">A Takala</name>
</author>
<author><name sortKey="Syrjanen, R" uniqKey="Syrjanen R">R Syrjanen</name>
</author>
<author><name sortKey="Jousimies Somer, H" uniqKey="Jousimies Somer H">H Jousimies-Somer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brook, I" uniqKey="Brook I">I Brook</name>
</author>
<author><name sortKey="Frazier, Eh" uniqKey="Frazier E">EH Frazier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brook, I" uniqKey="Brook I">I Brook</name>
</author>
<author><name sortKey="Yocum, P" uniqKey="Yocum P">P Yocum</name>
</author>
<author><name sortKey="Shah, K" uniqKey="Shah K">K Shah</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brook, I" uniqKey="Brook I">I Brook</name>
</author>
<author><name sortKey="Yocum, P" uniqKey="Yocum P">P Yocum</name>
</author>
<author><name sortKey="Shah, K" uniqKey="Shah K">K Shah</name>
</author>
<author><name sortKey="Feldman, B" uniqKey="Feldman B">B Feldman</name>
</author>
<author><name sortKey="Epstein, S" uniqKey="Epstein S">S Epstein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kaufman, E" uniqKey="Kaufman E">E Kaufman</name>
</author>
<author><name sortKey="Lamster, Ib" uniqKey="Lamster I">IB Lamster</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Haraldsson, G" uniqKey="Haraldsson G">G Haraldsson</name>
</author>
<author><name sortKey="Holbrook, Wp" uniqKey="Holbrook W">WP Holbrook</name>
</author>
<author><name sortKey="Kononen, E" uniqKey="Kononen E">E Könönen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ueyama, T" uniqKey="Ueyama T">T Ueyama</name>
</author>
<author><name sortKey="Kurono, Y" uniqKey="Kurono Y">Y Kurono</name>
</author>
<author><name sortKey="Shrabe, K" uniqKey="Shrabe K">K Shrabe</name>
</author>
<author><name sortKey="Takeshita, M" uniqKey="Takeshita M">M Takeshita</name>
</author>
<author><name sortKey="Magi, G" uniqKey="Magi G">G Magi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Faden, H" uniqKey="Faden H">H Faden</name>
</author>
<author><name sortKey="Duffy, L" uniqKey="Duffy L">L Duffy</name>
</author>
<author><name sortKey="Wasielewski, R" uniqKey="Wasielewski R">R Wasielewski</name>
</author>
<author><name sortKey="Wolf, J" uniqKey="Wolf J">J Wolf</name>
</author>
<author><name sortKey="Krystofik, D" uniqKey="Krystofik D">D Krystofik</name>
</author>
<author><name sortKey="Tung, Y" uniqKey="Tung Y">Y Tung</name>
</author>
<author><name sortKey="Tonawanda Williamsville, Pediatrics" uniqKey="Tonawanda Williamsville P">Pediatrics Tonawanda/ Williamsville</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Greene, Jc" uniqKey="Greene J">JC Greene</name>
</author>
<author><name sortKey="Vermillion, Jr" uniqKey="Vermillion J">JR Vermillion</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Silness, J" uniqKey="Silness J">J Silness</name>
</author>
<author><name sortKey="Loe, H" uniqKey="Loe H">H Löe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pearson, Wr" uniqKey="Pearson W">WR Pearson</name>
</author>
<author><name sortKey="Lipman, Dj" uniqKey="Lipman D">DJ Lipman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brook, I" uniqKey="Brook I">I Brook</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Litterio, M" uniqKey="Litterio M">M Litterio</name>
</author>
<author><name sortKey="Hernandez, C" uniqKey="Hernandez C">C Hernandez</name>
</author>
<author><name sortKey="Bernaldez, P" uniqKey="Bernaldez P">P Bernaldez</name>
</author>
<author><name sortKey="Fiorill, Ig" uniqKey="Fiorill I">IG Fiorill</name>
</author>
<author><name sortKey="Rubeglio, E" uniqKey="Rubeglio E">E Rubeglio</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kononen, E" uniqKey="Kononen E">E Könönen</name>
</author>
<author><name sortKey="Kanervo, A" uniqKey="Kanervo A">A Kanervo</name>
</author>
<author><name sortKey="Bryk, A" uniqKey="Bryk A">A Bryk</name>
</author>
<author><name sortKey="Asikainen, S" uniqKey="Asikainen S">S Asikainen</name>
</author>
<author><name sortKey="Jousimies Somer, H" uniqKey="Jousimies Somer H">H Jousimies-Somer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kolenbrander, Pe" uniqKey="Kolenbrander P">PE Kolenbrander</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kononen, E" uniqKey="Kononen E">E Könönen</name>
</author>
<author><name sortKey="Kanervo, A" uniqKey="Kanervo A">A Kanervo</name>
</author>
<author><name sortKey="Salminen, K" uniqKey="Salminen K">K Salminen</name>
</author>
<author><name sortKey="Jousimies Somer, H" uniqKey="Jousimies Somer H">H Jousimies-Somer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Haraldsson, G" uniqKey="Haraldsson G">G Haraldsson</name>
</author>
<author><name sortKey="Holbrook, Wp" uniqKey="Holbrook W">WP Holbrook</name>
</author>
<author><name sortKey="Kononen, E" uniqKey="Kononen E">E Könönen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="George, Ks" uniqKey="George K">KS George</name>
</author>
<author><name sortKey="Reynolds, Ma" uniqKey="Reynolds M">MA Reynolds</name>
</author>
<author><name sortKey="Falkler, Wa" uniqKey="Falkler W">WA Falkler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thurnheer, T" uniqKey="Thurnheer T">T Thurnheer</name>
</author>
<author><name sortKey="Guggenheim, B" uniqKey="Guggenheim B">B Guggenheim</name>
</author>
<author><name sortKey="Gruica, B" uniqKey="Gruica B">B Gruica</name>
</author>
<author><name sortKey="Gmur, R" uniqKey="Gmur R">R Gmür</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Int J Med Sci</journal-id>
<journal-id journal-id-type="iso-abbrev">Int J Med Sci</journal-id>
<journal-id journal-id-type="publisher-id">ijms</journal-id>
<journal-title-group><journal-title>International Journal of Medical Sciences</journal-title>
</journal-title-group>
<issn pub-type="epub">1449-1907</issn>
<publisher><publisher-name>Ivyspring International Publisher</publisher-name>
<publisher-loc>Sydney</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">22606045</article-id>
<article-id pub-id-type="pmc">3354330</article-id>
<article-id pub-id-type="doi">10.7150/ijms.4382</article-id>
<article-id pub-id-type="publisher-id">ijmsv09p0256</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Paper</subject>
</subj-group>
</article-categories>
<title-group><article-title>Relationship between Oral Anaerobic Bacteria and Otitis Media with Effusion</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Topcuoglu</surname>
<given-names>Nursen</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="corresp" rid="FNA_envelop">✉</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Keskin</surname>
<given-names>Fahriye</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ciftci</surname>
<given-names>Sevgi</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Paltura</surname>
<given-names>Ceki</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kulekci</surname>
<given-names>Mehmet</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ustek</surname>
<given-names>Duran</given-names>
</name>
<xref ref-type="aff" rid="A3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kulekci</surname>
<given-names>Guven</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
</contrib-group>
<aff id="A1">1. Oral Microbiology Laboratory, Istanbul University Faculty of Dentistry, Istanbul, Turkey;</aff>
<aff id="A2">2. Department of Otorhinolaryngology, Head and Neck Surgery, Taksim Training and Research Hospital, Istanbul, Turkey;</aff>
<aff id="A3">3. Department of Genetics, Institute for Experimental Medicine, Istanbul Faculty of Medicine, Istanbul, Turkey.</aff>
<author-notes><corresp id="FNA_envelop">✉ Corresponding author: Tel: +902124142595; Fax: +902125312230, E-mail address: <email>nursentopcuoglu@yahoo.com</email>
(N. Topuoglu).</corresp>
<fn fn-type="conflict"><p>Competing Interests: The authors have declared that no competing interest exists.</p>
</fn>
</author-notes>
<pub-date pub-type="collection"><year>2012</year>
</pub-date>
<pub-date pub-type="epub"><day>7</day>
<month>5</month>
<year>2012</year>
</pub-date>
<volume>9</volume>
<issue>3</issue>
<fpage>256</fpage>
<lpage>261</lpage>
<history><date date-type="received"><day>19</day>
<month>3</month>
<year>2012</year>
</date>
<date date-type="accepted"><day>3</day>
<month>5</month>
<year>2012</year>
</date>
</history>
<permissions><copyright-statement>© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.</copyright-statement>
<copyright-year>2012</copyright-year>
</permissions>
<abstract><p><bold>Objective:</bold>
In this study hypothesing the translocation of oral bacteria from oropharynx into the middle ear cavity may be involved in the pathogenesis of otitis media with effusion (OME), we aimed to investigate the presence and similarity of <italic>Fusobacterium nucleatum</italic>
and <italic>Treponema denticola</italic>
in saliva, nasopharyngeal secretion and the middle ear effusion samples from the children with OME.</p>
<p><bold>Methods:</bold>
Totally 20 children with OME undergoing myringotomy and ventilation tube placement were attended. Stimulated saliva samples were collected after otorhinolaryngological and oral examinations were done. The middle ear effusion and nasopharyngeal secretions were collected during the operations. The presence of <italic>F. nucleatum</italic>
and <italic>T. denticola</italic>
were detected using 16SrRNA-based PCR. The clonal similarities of the bacteria were detected in the samples which the same bacteria had been detected in each samples of the same child. After DNA sequencing, clonal similarity was determined by 16SrRNA gene clone library analysis. The sequences from each clone were compared with similar sequences of reference organisms by FASTA search.</p>
<p><bold>Results:</bold>
<italic> T. denticola</italic>
was detected only in four (20%) saliva and in one (5%) nasopharyngeal sample. <italic>F. nucleatum</italic>
was detected in 11 (55%) saliva, eight (40%) nasopharyngeal and six (30%) middle ear effusion samples. Sequences from <italic>F.nucleatum</italic>
clones derived from three different anatomic sites within patients were similar in 33% of OME patients, indicating their genetic relatedness.</p>
<p><bold>Conclusions:</bold>
Bacteria involved in this process most likely originate from the oropharynx since they show a close genetic relatedness with their oropharyngeal counterparts.</p>
</abstract>
<kwd-group><kwd>Middle ear effusion</kwd>
<kwd>saliva</kwd>
<kwd><italic>Fusobacterium nucleatum</italic>
</kwd>
<kwd>sequence analysis.</kwd>
</kwd-group>
</article-meta>
</front>
<floats-group><table-wrap id="T1" position="float"><label>Table 1</label>
<caption><p>Number and percentage of OME patient with positive samples distributed over respective locations.</p>
</caption>
<table frame="hsides" rules="groups"><thead valign="top"><tr><th rowspan="1" colspan="1"></th>
<th colspan="2" rowspan="1"><italic>F.nucleatum</italic>
</th>
<th colspan="2" rowspan="1"><italic>T.denticola</italic>
</th>
</tr>
<tr><th rowspan="1" colspan="1">Location</th>
<th rowspan="1" colspan="1">ƒ</th>
<th rowspan="1" colspan="1">%</th>
<th rowspan="1" colspan="1">ƒ</th>
<th rowspan="1" colspan="1">%</th>
</tr>
</thead>
<tbody valign="top"><tr><td rowspan="1" colspan="1">Saliva+NS+MEE</td>
<td rowspan="1" colspan="1">6</td>
<td rowspan="1" colspan="1">30</td>
<td rowspan="1" colspan="1">0</td>
<td rowspan="1" colspan="1">0</td>
</tr>
<tr><td rowspan="1" colspan="1">Only Saliva+NS</td>
<td rowspan="1" colspan="1">2</td>
<td rowspan="1" colspan="1">10</td>
<td rowspan="1" colspan="1">0</td>
<td rowspan="1" colspan="1">0</td>
</tr>
<tr><td rowspan="1" colspan="1">Only Saliva</td>
<td rowspan="1" colspan="1">3</td>
<td rowspan="1" colspan="1">15</td>
<td rowspan="1" colspan="1">4</td>
<td rowspan="1" colspan="1">20</td>
</tr>
<tr><td rowspan="1" colspan="1">Only NS</td>
<td rowspan="1" colspan="1">0</td>
<td rowspan="1" colspan="1">0</td>
<td rowspan="1" colspan="1">1</td>
<td rowspan="1" colspan="1">5</td>
</tr>
<tr><td rowspan="1" colspan="1">Only MEE</td>
<td rowspan="1" colspan="1">0</td>
<td rowspan="1" colspan="1">0</td>
<td rowspan="1" colspan="1">0</td>
<td rowspan="1" colspan="1">0</td>
</tr>
<tr><td rowspan="1" colspan="1">TOTAL</td>
<td rowspan="1" colspan="1">11</td>
<td rowspan="1" colspan="1">55</td>
<td rowspan="1" colspan="1">5</td>
<td rowspan="1" colspan="1">25</td>
</tr>
</tbody>
</table>
<table-wrap-foot><fn><p>ƒ, Frequency; NS, nasopharyngeal secretion; MEE, middle ear effusion.</p>
</fn>
</table-wrap-foot>
</table-wrap>
<table-wrap id="T2" position="float"><label>Table 2</label>
<caption><p>Clinical conditions of the children according to presence of <italic>F.nucleatum</italic>
in their MEE samples.</p>
</caption>
<table frame="hsides" rules="groups"><thead valign="top"><tr><th rowspan="1" colspan="1"><italic>F.nucletum</italic>
positive in MEE samples</th>
<th rowspan="1" colspan="1">n</th>
<th rowspan="1" colspan="1">Mixed dentition</th>
<th rowspan="1" colspan="1">Age</th>
<th rowspan="1" colspan="1">Plaque index</th>
<th rowspan="1" colspan="1">Gingival index</th>
<th rowspan="1" colspan="1">Antibiotic usage</th>
</tr>
</thead>
<tbody valign="top"><tr><td rowspan="1" colspan="1">Yes</td>
<td rowspan="1" colspan="1">6</td>
<td rowspan="1" colspan="1">5 (83%)</td>
<td rowspan="1" colspan="1">7.7 ± 1.9</td>
<td rowspan="1" colspan="1">1,36 ± 0,75</td>
<td rowspan="1" colspan="1">0,11 ± 0,20</td>
<td rowspan="1" colspan="1">5 (83%)</td>
</tr>
<tr><td rowspan="1" colspan="1">No</td>
<td rowspan="1" colspan="1">14</td>
<td rowspan="1" colspan="1">11 (79%)</td>
<td rowspan="1" colspan="1">7.9 ± 1.9</td>
<td rowspan="1" colspan="1">1,37 ± 0,57</td>
<td rowspan="1" colspan="1">0,37 ± 0,39</td>
<td rowspan="1" colspan="1">7 (50%)</td>
</tr>
<tr><td rowspan="1" colspan="1">Total</td>
<td rowspan="1" colspan="1">20</td>
<td rowspan="1" colspan="1">16 (80%)</td>
<td rowspan="1" colspan="1">7.8 ± 2</td>
<td rowspan="1" colspan="1">1,36 ± 0,29</td>
<td rowspan="1" colspan="1">0,29 ± 0,36</td>
<td rowspan="1" colspan="1">12 (60%)</td>
</tr>
</tbody>
</table>
</table-wrap>
<table-wrap id="T3" position="float"><label>Table 3</label>
<caption><p>The clonal similarities of the <italic>Fusobacterium nucleatum</italic>
obtained from the samples with reference organisms.</p>
</caption>
<table frame="hsides" rules="groups"><thead valign="top"><tr><th rowspan="2" colspan="1">Patient No</th>
<th rowspan="2" colspan="1">Sample</th>
<th rowspan="2" colspan="1">Sequence No</th>
<th colspan="3" rowspan="1"><italic>F.nucleatum</italic>
reference strains</th>
</tr>
<tr><th rowspan="1" colspan="1">ATCC 49256</th>
<th rowspan="1" colspan="1">ATCC 25586</th>
<th rowspan="1" colspan="1">ATCC 33693</th>
</tr>
</thead>
<tbody valign="top"><tr><td colspan="2" rowspan="1"><bold><italic>Triple samples</italic>
</bold>
</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1">1</td>
<td rowspan="1" colspan="1">MEE</td>
<td rowspan="1" colspan="1">801</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">Saliva</td>
<td rowspan="1" colspan="1">807</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
</tr>
<tr><td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">NS</td>
<td rowspan="1" colspan="1">813</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
</tr>
<tr><td rowspan="1" colspan="1">4</td>
<td rowspan="1" colspan="1">MEE</td>
<td rowspan="1" colspan="1">802</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">Saliva</td>
<td rowspan="1" colspan="1">808</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">NS</td>
<td rowspan="1" colspan="1">814</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1">7</td>
<td rowspan="1" colspan="1">MEE</td>
<td rowspan="1" colspan="1">803</td>
<td rowspan="1" colspan="1">100</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">Saliva</td>
<td rowspan="1" colspan="1">809</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
</tr>
<tr><td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">NS</td>
<td rowspan="1" colspan="1">815</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1">11</td>
<td rowspan="1" colspan="1">MEE</td>
<td rowspan="1" colspan="1">804</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">Saliva</td>
<td rowspan="1" colspan="1">810</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
</tr>
<tr><td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">NS</td>
<td rowspan="1" colspan="1">816</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
</tr>
<tr><td rowspan="1" colspan="1">12</td>
<td rowspan="1" colspan="1">MEE</td>
<td rowspan="1" colspan="1">805</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">Saliva</td>
<td rowspan="1" colspan="1">811</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
</tr>
<tr><td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">NS</td>
<td rowspan="1" colspan="1">817</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">Mixed</td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1">13</td>
<td rowspan="1" colspan="1">MEE</td>
<td rowspan="1" colspan="1">806</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">Saliva</td>
<td rowspan="1" colspan="1">812</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">NS</td>
<td rowspan="1" colspan="1">818</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td colspan="2" rowspan="1"><bold><italic>Double samples</italic>
</bold>
</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1">3</td>
<td rowspan="1" colspan="1">Saliva</td>
<td rowspan="1" colspan="1">819</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">NS</td>
<td rowspan="1" colspan="1">821</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
<td rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1">9</td>
<td rowspan="1" colspan="1">Saliva</td>
<td rowspan="1" colspan="1">820</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
</tr>
<tr><td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">NS</td>
<td rowspan="1" colspan="1">822</td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1"></td>
<td rowspan="1" colspan="1">100</td>
</tr>
</tbody>
</table>
<table-wrap-foot><fn><p>NS: nasopharyngeal secretion; MEE: middle ear effusion.</p>
</fn>
</table-wrap-foot>
</table-wrap>
</floats-group>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/EdenteV2/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002E36 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 002E36 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Santé |area= EdenteV2 |flux= Pmc |étape= Curation |type= RBID |clé= PMC:3354330 |texte= Relationship between Oral Anaerobic Bacteria and Otitis Media with Effusion }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:22606045" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \ | NlmPubMed2Wicri -a EdenteV2
![]() | This area was generated with Dilib version V0.6.32. | ![]() |