Role of Salivary and Candidal Proteins in Denture Stomatitis; an exploratory proteomic analysis
Identifieur interne : 002647 ( Pmc/Curation ); précédent : 002646; suivant : 002648Role of Salivary and Candidal Proteins in Denture Stomatitis; an exploratory proteomic analysis
Auteurs : Warren C. Byrd [États-Unis] ; Sarah Schwartz-Baxter [États-Unis] ; Jim Carlson [États-Unis] ; Silvana Barros [États-Unis] ; Steven Offenbacher [États-Unis] ; Sompop Bencharit [États-Unis]Source :
- Molecular bioSystems [ 1742-206X ] ; 2014.
Abstract
Denture stomatitis, inflammation and redness beneath a denture, affects nearly half of all denture wearers. Candida organism, the presence of a denture, saliva, and host immunity are the key etiological factors for the condition. The role of salivary proteins in denture stomatitis is not clear. In this study 30 edentulous subjects wearing a maxillary complete denture were recruited. Unstimulated whole saliva from each subject was collected and pooled into two groups (n=15 each); healthy and stomatitis (Newton classification II and III). Label-free multidimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS) proteomics on two mass spectrometry platforms were used to determine peptide mass differences between control and stomatitis groups. Cluster analysis and principal component analysis were used to determine differential expression among the groups. The two proteomic platforms identified 97 and 176 proteins (ANOVA; p<0.01) differentially expressed among the healthy, type 2 and 3 stomatitis groups. Three proteins including carbonic anhydrase 6, cystatin C, and cystatin SN were found to be the same as previous study. Salivary proteomic profiles of patients with denture stomatitis were found to be uniquely different from controls. Analysis of protein components suggests that certain salivary proteins may predispose some patients to denture stomatitis while others are believed to be involved in the reaction to fungal infection. Analysis of candidal proteins suggest that multiple species of candidal organisms play a role in denture stomatitis.
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DOI: 10.1039/c4mb00185k
PubMed: 24947908
PubMed Central: 4119604
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Byrd</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, School of Dentistry, Department of Pharmacology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Prosthodontics, School of Dentistry, Department of Pharmacology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599</wicri:regionArea></affiliation></author><author><name sortKey="Schwartz Baxter, Sarah" sort="Schwartz Baxter, Sarah" uniqKey="Schwartz Baxter S" first="Sarah" last="Schwartz-Baxter">Sarah Schwartz-Baxter</name><affiliation wicri:level="1"><nlm:aff id="A2">David H. Murdock Research Institute, North Carolina Research Campus, Kannapolis, NC 28081, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>David H. Murdock Research Institute, North Carolina Research Campus, Kannapolis, NC 28081</wicri:regionArea></affiliation></author><author><name sortKey="Carlson, Jim" sort="Carlson, Jim" uniqKey="Carlson J" first="Jim" last="Carlson">Jim Carlson</name><affiliation wicri:level="1"><nlm:aff id="A2">David H. Murdock Research Institute, North Carolina Research Campus, Kannapolis, NC 28081, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>David H. 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Candida organism, the presence of a denture, saliva, and host immunity are the key etiological factors for the condition. The role of salivary proteins in denture stomatitis is not clear. In this study 30 edentulous subjects wearing a maxillary complete denture were recruited. Unstimulated whole saliva from each subject was collected and pooled into two groups (n=15 each); healthy and stomatitis (Newton classification II and III). Label-free multidimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS) proteomics on two mass spectrometry platforms were used to determine peptide mass differences between control and stomatitis groups. Cluster analysis and principal component analysis were used to determine differential expression among the groups. The two proteomic platforms identified 97 and 176 proteins (ANOVA; p<0.01) differentially expressed among the healthy, type 2 and 3 stomatitis groups. Three proteins including carbonic anhydrase 6, cystatin C, and cystatin SN were found to be the same as previous study. Salivary proteomic profiles of patients with denture stomatitis were found to be uniquely different from controls. Analysis of protein components suggests that certain salivary proteins may predispose some patients to denture stomatitis while others are believed to be involved in the reaction to fungal infection. Analysis of candidal proteins suggest that multiple species of candidal organisms play a role in denture stomatitis.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">101251620</journal-id><journal-id journal-id-type="pubmed-jr-id">33015</journal-id><journal-id journal-id-type="nlm-ta">Mol Biosyst</journal-id><journal-id journal-id-type="iso-abbrev">Mol Biosyst</journal-id><journal-title-group><journal-title>Molecular bioSystems</journal-title></journal-title-group><issn pub-type="ppub">1742-206X</issn><issn pub-type="epub">1742-2051</issn></journal-meta><article-meta><article-id pub-id-type="pmid">24947908</article-id><article-id pub-id-type="pmc">4119604</article-id><article-id pub-id-type="doi">10.1039/c4mb00185k</article-id><article-id pub-id-type="manuscript">NIHMS607081</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Role of Salivary and Candidal Proteins in Denture Stomatitis; an exploratory proteomic analysis</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Byrd</surname><given-names>Warren C.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Schwartz-Baxter</surname><given-names>Sarah</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Carlson</surname><given-names>Jim</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Barros</surname><given-names>Silvana</given-names></name><xref ref-type="aff" rid="A3">3</xref></contrib><contrib contrib-type="author"><name><surname>Offenbacher</surname><given-names>Steven</given-names></name><xref ref-type="aff" rid="A3">3</xref></contrib><contrib contrib-type="author"><name><surname>Bencharit</surname><given-names>Sompop</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref rid="FN1" ref-type="author-notes">*</xref></contrib></contrib-group><aff id="A1"><label>1</label>Department of Prosthodontics, School of Dentistry, Department of Pharmacology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599, USA</aff><aff id="A2"><label>2</label>David H. Murdock Research Institute, North Carolina Research Campus, Kannapolis, NC 28081, USA</aff><aff id="A3"><label>3</label>Department of Periodontology, School of Dentistry, University of North Carolina, Chapel Hill, NC 27599, USA</aff><author-notes><corresp id="FN1"><label>*</label>Address correspondence to: Sompop Bencharit, DDS, MS, PhD, FACP, Department of Prosthodontics, School of Dentistry; and Department of Pharmacology, School of Medicine, CB#7450 Chapel Hill, NC 27599-7450, Tel: 919-843-8734, Fax: 919-966-3821, <email>Sompop_Bencharit@dentistry.unc.edu</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>21</day><month>6</month><year>2014</year></pub-date><pub-date pub-type="ppub"><day>29</day><month>7</month><year>2014</year></pub-date><pub-date pub-type="pmc-release"><day>29</day><month>7</month><year>2015</year></pub-date><volume>10</volume><issue>9</issue><fpage>2299</fpage><lpage>2304</lpage><pmc-comment>elocation-id from pubmed: 10.1039/c4mb00185k</pmc-comment>
<abstract><p id="P1">Denture stomatitis, inflammation and redness beneath a denture, affects nearly half of all denture wearers. Candida organism, the presence of a denture, saliva, and host immunity are the key etiological factors for the condition. The role of salivary proteins in denture stomatitis is not clear. In this study 30 edentulous subjects wearing a maxillary complete denture were recruited. Unstimulated whole saliva from each subject was collected and pooled into two groups (n=15 each); healthy and stomatitis (Newton classification II and III). Label-free multidimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS) proteomics on two mass spectrometry platforms were used to determine peptide mass differences between control and stomatitis groups. Cluster analysis and principal component analysis were used to determine differential expression among the groups. The two proteomic platforms identified 97 and 176 proteins (ANOVA; p<0.01) differentially expressed among the healthy, type 2 and 3 stomatitis groups. Three proteins including carbonic anhydrase 6, cystatin C, and cystatin SN were found to be the same as previous study. Salivary proteomic profiles of patients with denture stomatitis were found to be uniquely different from controls. Analysis of protein components suggests that certain salivary proteins may predispose some patients to denture stomatitis while others are believed to be involved in the reaction to fungal infection. Analysis of candidal proteins suggest that multiple species of candidal organisms play a role in denture stomatitis.</p></abstract><kwd-group><kwd>dentures</kwd><kwd>mass spectrometry</kwd><kwd>proteomics</kwd><kwd>saliva</kwd><kwd>stomatitis</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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