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Microbiota of deciduous endodontic infections analyzed by MDA and Checkerboard DNA-DNA hybridization

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Microbiota of deciduous endodontic infections analyzed by MDA and Checkerboard DNA-DNA hybridization

Auteurs : Wlf Tavares [Brésil] ; Lc Neves De Brito [Brésil] ; Rp Teles [États-Unis] ; Mla Massara [Brésil] ; Ap Ribeiro Sobrinho [Brésil] ; Ad Haffajee [États-Unis] ; Ss Socransky [États-Unis] ; Fr Teles [États-Unis]

Source :

International endodontic journal [ 0143-2885 ] ; 2010.

RBID : PMC:3177302

Abstract

Aims

To evaluate the microbiota of endodontic infections in deciduous teeth by checkerboard DNA-DNA hybridization after uniform amplification of DNA in samples by multiple displacement amplification (MDA).

Methodology

Forty samples from the root canal system of deciduous teeth exhibiting pulp necrosis with or without radiographically detectable periradicular/interadicular bone resorption were collected and 32 were analyzed, with 3 individuals contributing 2 samples; these were MDA- amplified and analyzed by Checkerboard DNA-DNA hybridization for levels of 83 bacterial taxa. Two outcome measures were used: the percentage of teeth colonized by each species; and the mean proportion of each bacterial taxon present across all samples were computed.

Results

The mean amount of DNA in the samples prior to amplification was 5.2 (± 4.7) ng and 6.1 (± 2.3) μg after MDA. The mean number of species detected per sample was 19 (± 4) (range: 3–66) to the nearest whole number. The most prevalent taxa were Prevotella intermedia (96.9%), Neisseria mucosa (65.6%), Prevotella nigrescens (56.2%) and Tannerella forsythia (56.2%). Aggregatibacter (Haemophilus) aphrophilus and Helicobacter pylori were not detected. P. intermedia (10%), Prevotella tannerae (7%) and Prevotella nigrescens (4.3%) presented the highest mean proportions of the target species averaged across the positive samples.

Conclusion

Root canals of infected deciduous teeth had a diverse bacterial population. Prevotella sp were commonly found with P. intermedia, Prevotella tannerae and Prevotella nigrescens among the most prominent species detected.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177302

DOI: 10.1111/j.1365-2591.2010.01805.x
PubMed: 21083570
PubMed Central: 3177302

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<author><name sortKey="Tavares, Wlf" sort="Tavares, Wlf" uniqKey="Tavares W" first="Wlf" last="Tavares">Wlf Tavares</name>
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<series><title level="j">International endodontic journal</title>
<idno type="ISSN">0143-2885</idno>
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<imprint><date when="2010">2010</date>
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<front><div type="abstract" xml:lang="en"><sec id="S1"><title>Aims</title>
<p id="P1">To evaluate the microbiota of endodontic infections in deciduous teeth by checkerboard DNA-DNA hybridization after uniform amplification of DNA in samples by multiple displacement amplification (MDA).</p>
</sec>
<sec sec-type="methods" id="S2"><title>Methodology</title>
<p id="P2">Forty samples from the root canal system of deciduous teeth exhibiting pulp necrosis with or without radiographically detectable periradicular/interadicular bone resorption were collected and 32 were analyzed, with 3 individuals contributing 2 samples; these were MDA- amplified and analyzed by Checkerboard DNA-DNA hybridization for levels of 83 bacterial taxa. Two outcome measures were used: the percentage of teeth colonized by each species; and the mean proportion of each bacterial taxon present across all samples were computed.</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">The mean amount of DNA in the samples prior to amplification was 5.2 (± 4.7) ng and 6.1 (± 2.3) μg after MDA. The mean number of species detected per sample was 19 (± 4) (range: 3–66) to the nearest whole number. The most prevalent taxa were <italic>Prevotella intermedia</italic>
 (96.9%), <italic>Neisseria mucosa</italic>
 (65.6%), <italic>Prevotella nigrescens</italic>
 (56.2%) and <italic>Tannerella forsythia</italic>
 (56.2%). <italic>Aggregatibacter (Haemophilus) aphrophilus</italic>
 and <italic>Helicobacter pylori</italic>
 were not detected. <italic>P. intermedia</italic>
 (10%), <italic>Prevotella tannerae</italic>
 (7%) and <italic>Prevotella nigrescens</italic>
 (4.3%) presented the highest mean proportions of the target species averaged across the positive samples.</p>
</sec>
<sec id="S4"><title>Conclusion</title>
<p id="P4">Root canals of infected deciduous teeth had a diverse bacterial population. <italic>Prevotella</italic>
 sp were commonly found with <italic>P. intermedia, Prevotella tannerae</italic>
 and <italic>Prevotella nigrescens</italic>
 among the most prominent species detected.</p>
</sec>
</div>
</front>
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<pmc article-type="research-article" xml:lang="en"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <pmc-dir>properties manuscript</pmc-dir>
  <front><journal-meta><journal-id journal-id-type="nlm-journal-id">8004996</journal-id>
<journal-id journal-id-type="pubmed-jr-id">4259</journal-id>
<journal-id journal-id-type="nlm-ta">Int Endod J</journal-id>
<journal-title-group><journal-title>International endodontic journal</journal-title>
</journal-title-group>
<issn pub-type="ppub">0143-2885</issn>
<issn pub-type="epub">1365-2591</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">21083570</article-id>
<article-id pub-id-type="pmc">3177302</article-id>
<article-id pub-id-type="doi">10.1111/j.1365-2591.2010.01805.x</article-id>
<article-id pub-id-type="manuscript">NIHMS270460</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Microbiota of deciduous endodontic infections analyzed by MDA and Checkerboard DNA-DNA hybridization</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Tavares</surname>
<given-names>WLF</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>de Brito</surname>
<given-names>LC Neves</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Teles</surname>
<given-names>RP</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Massara</surname>
<given-names>MLA</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sobrinho</surname>
<given-names>AP Ribeiro</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Haffajee</surname>
<given-names>AD</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Socransky</surname>
<given-names>SS</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Teles</surname>
<given-names>FR</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>1</label>
Department of Periodontology, The Forsyth Institute, Boston, Massachusetts, USA</aff>
<aff id="A2"><label>2</label>
Federal University of Minas Gerais School of Dentistry, Belo Horizonte, Minas Gerais, Brazil</aff>
<author-notes><corresp id="CR1"><bold>Correspondence:</bold>
 Dr. Antonio Paulino Ribeiro Sobrinho, Departamento de Odontologia Restauradora, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil, Av. Antonio Carlos 6627, CEP 30.161-970, Belo Horizonte, MG, Brasil. Tel. 55-31-34992470, <email>sobrinho.bhz@terra.com.br</email>
</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>14</day>
<month>2</month>
<year>2011</year>
</pub-date>
<pub-date pub-type="epub"><day>17</day>
<month>11</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="ppub"><month>3</month>
<year>2011</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>1</day>
<month>3</month>
<year>2012</year>
</pub-date>
<volume>44</volume>
<issue>3</issue>
<fpage>225</fpage>
<lpage>235</lpage>
<abstract><sec id="S1"><title>Aims</title>
<p id="P1">To evaluate the microbiota of endodontic infections in deciduous teeth by checkerboard DNA-DNA hybridization after uniform amplification of DNA in samples by multiple displacement amplification (MDA).</p>
</sec>
<sec sec-type="methods" id="S2"><title>Methodology</title>
<p id="P2">Forty samples from the root canal system of deciduous teeth exhibiting pulp necrosis with or without radiographically detectable periradicular/interadicular bone resorption were collected and 32 were analyzed, with 3 individuals contributing 2 samples; these were MDA- amplified and analyzed by Checkerboard DNA-DNA hybridization for levels of 83 bacterial taxa. Two outcome measures were used: the percentage of teeth colonized by each species; and the mean proportion of each bacterial taxon present across all samples were computed.</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">The mean amount of DNA in the samples prior to amplification was 5.2 (± 4.7) ng and 6.1 (± 2.3) μg after MDA. The mean number of species detected per sample was 19 (± 4) (range: 3–66) to the nearest whole number. The most prevalent taxa were <italic>Prevotella intermedia</italic>
 (96.9%), <italic>Neisseria mucosa</italic>
 (65.6%), <italic>Prevotella nigrescens</italic>
 (56.2%) and <italic>Tannerella forsythia</italic>
 (56.2%). <italic>Aggregatibacter (Haemophilus) aphrophilus</italic>
 and <italic>Helicobacter pylori</italic>
 were not detected. <italic>P. intermedia</italic>
 (10%), <italic>Prevotella tannerae</italic>
 (7%) and <italic>Prevotella nigrescens</italic>
 (4.3%) presented the highest mean proportions of the target species averaged across the positive samples.</p>
</sec>
<sec id="S4"><title>Conclusion</title>
<p id="P4">Root canals of infected deciduous teeth had a diverse bacterial population. <italic>Prevotella</italic>
 sp were commonly found with <italic>P. intermedia, Prevotella tannerae</italic>
 and <italic>Prevotella nigrescens</italic>
 among the most prominent species detected.</p>
</sec>
</abstract>
<funding-group><award-group><funding-source country="United States">National Institute of Dental and Craniofacial Research  : NIDCR</funding-source>
<award-id>T32 DE007327-09 || DE</award-id>
</award-group>
<award-group><funding-source country="United States">National Institute of Dental and Craniofacial Research  : NIDCR</funding-source>
<award-id>R01 DE012108-08 || DE</award-id>
</award-group>
</funding-group>
</article-meta>
</front>
</pmc>
</record>

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Microbiota of deciduous endodontic infections analyzed by MDA and Checkerboard DNA-DNA hybridization

Microbiota of deciduous endodontic infections analyzed by MDA and Checkerboard DNA-DNA hybridization

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Abstract

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