Microstructured zirconia surfaces modulate osteogenic marker genes in human primary osteoblasts
Identifieur interne : 000D16 ( Pmc/Curation ); précédent : 000D15; suivant : 000D17Microstructured zirconia surfaces modulate osteogenic marker genes in human primary osteoblasts
Auteurs : Claudia Bergemann [Allemagne] ; Kathrin Duske [Allemagne] ; J. Barbara Nebe [Allemagne] ; André Schöne [Allemagne] ; Ulrike Bulnheim [Allemagne] ; Hermann Seitz [Allemagne] ; Jens Fischer [Allemagne, Suisse]Source :
- Journal of Materials Science. Materials in Medicine [ 0957-4530 ] ; 2015.
Abstract
In dentistry, zirconia has been used since the early 1990s for endodontic posts, more recently for implant abutments and frameworks for fixed dental prostheses. Zirconia is biocompatible and mechanically strong enough to serve as implant material for oral implants. Although several zirconia implant systems are available, currently the scientific and clinical data for zirconia implants are not sufficient to recommend them for routine clinical use. Here the influence of microstructured yttria-stabilized zirconia (YZ) on human primary osteoblast (HOB) behavior was determined. YZ surfaces were treated by sandblasting (YZ-S), acid etching (YZ-SE) and additionally heat treatment (YZ-SEH). Morphological changes of HOB were determined by scanning electron microscopy. Actin cytoskeleton was investigated by laser scanning microscopy and analyzed by novel actin quantification software. Differentiation of HOB was determined by real time RT-PCR. Improved mechanical interlocking of primary HOB into the porous microstructure of the acid etched and additionally heat treated YZ-surfaces correlates with drastically increased osteocalcin (OCN) gene expression. In particular, OCN was considerably elevated in primary HOB after 3 days on YZ-SE (13-fold) as well as YZ-SEH (12-fold) surfaces. Shorter actin filaments without any favored orientation on YZ-SE and YZ-SEH surfaces are associated with higher roughness (Ra) values. Topographically modified yttria-stabilized zirconia is a likely material for dental implants with cell stimulating properties achieving or actually exceeding those of titanium.
Url:
DOI: 10.1007/s10856-014-5350-x
PubMed: 25578704
PubMed Central: 4289972
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<front><div type="abstract" xml:lang="en"><p>In dentistry, zirconia has been used since the early 1990s for endodontic posts, more recently for implant abutments and frameworks for fixed dental prostheses. Zirconia is biocompatible and mechanically strong enough to serve as implant material for oral implants. Although several zirconia implant systems are available, currently the scientific and clinical data for zirconia implants are not sufficient to recommend them for routine clinical use. Here the influence of microstructured yttria-stabilized zirconia (YZ) on human primary osteoblast (HOB) behavior was determined. YZ surfaces were treated by sandblasting (YZ-S), acid etching (YZ-SE) and additionally heat treatment (YZ-SEH). Morphological changes of HOB were determined by scanning electron microscopy. Actin cytoskeleton was investigated by laser scanning microscopy and analyzed by novel actin quantification software. Differentiation of HOB was determined by real time RT-PCR. Improved mechanical interlocking of primary HOB into the porous microstructure of the acid etched and additionally heat treated YZ-surfaces correlates with drastically increased osteocalcin (OCN) gene expression. In particular, OCN was considerably elevated in primary HOB after 3 days on YZ-SE (13-fold) as well as YZ-SEH (12-fold) surfaces. Shorter actin filaments without any favored orientation on YZ-SE and YZ-SEH surfaces are associated with higher roughness (R<sub>a</sub>
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<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Lindhe, J" uniqKey="Lindhe J">J Lindhe</name>
</author>
<author><name sortKey="Lang, Np" uniqKey="Lang N">NP Lang</name>
</author>
<author><name sortKey="Karring, T" uniqKey="Karring T">T Karring</name>
</author>
<author><name sortKey="Berglundh, T" uniqKey="Berglundh T">T Berglundh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Avivi Arber, L" uniqKey="Avivi Arber L">L Avivi-Arber</name>
</author>
<author><name sortKey="Zarb, Ga" uniqKey="Zarb G">GA Zarb</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gibbard, Ll" uniqKey="Gibbard L">LL Gibbard</name>
</author>
<author><name sortKey="Zarb, G" uniqKey="Zarb G">G Zarb</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gomez Roman, G" uniqKey="Gomez Roman G">G Gomez-Roman</name>
</author>
<author><name sortKey="Schulte, W" uniqKey="Schulte W">W Schulte</name>
</author>
<author><name sortKey="D Oedt, B" uniqKey="D Oedt B">B d’Hoedt</name>
</author>
<author><name sortKey="Axman Krcmar, D" uniqKey="Axman Krcmar D">D Axman-Krcmar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Henry, Pj" uniqKey="Henry P">PJ Henry</name>
</author>
<author><name sortKey="Laney, Wr" uniqKey="Laney W">WR Laney</name>
</author>
<author><name sortKey="Jemt, T" uniqKey="Jemt T">T Jemt</name>
</author>
<author><name sortKey="Harris, D" uniqKey="Harris D">D Harris</name>
</author>
<author><name sortKey="Krogh, Ph" uniqKey="Krogh P">PH Krogh</name>
</author>
<author><name sortKey="Polizzi, G" uniqKey="Polizzi G">G Polizzi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Krennmair, G" uniqKey="Krennmair G">G Krennmair</name>
</author>
<author><name sortKey="Schmidinger, S" uniqKey="Schmidinger S">S Schmidinger</name>
</author>
<author><name sortKey="Waldenberger, O" uniqKey="Waldenberger O">O Waldenberger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Laney, Wr" uniqKey="Laney W">WR Laney</name>
</author>
<author><name sortKey="Jemt, T" uniqKey="Jemt T">T Jemt</name>
</author>
<author><name sortKey="Harris, D" uniqKey="Harris D">D Harris</name>
</author>
<author><name sortKey="Henry, Pj" uniqKey="Henry P">PJ Henry</name>
</author>
<author><name sortKey="Krogh, Ph" uniqKey="Krogh P">PH Krogh</name>
</author>
<author><name sortKey="Polizzi, G" uniqKey="Polizzi G">G Polizzi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Malevez, C" uniqKey="Malevez C">C Malevez</name>
</author>
<author><name sortKey="Hermans, M" uniqKey="Hermans M">M Hermans</name>
</author>
<author><name sortKey="Daelemans, P" uniqKey="Daelemans P">P Daelemans</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Priest, G" uniqKey="Priest G">G Priest</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Renouard, F" uniqKey="Renouard F">F Renouard</name>
</author>
<author><name sortKey="Nisand, D" uniqKey="Nisand D">D Nisand</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Scheller, H" uniqKey="Scheller H">H Scheller</name>
</author>
<author><name sortKey="Urgell, Jp" uniqKey="Urgell J">JP Urgell</name>
</author>
<author><name sortKey="Kultje, C" uniqKey="Kultje C">C Kultje</name>
</author>
<author><name sortKey="Klineberg, I" uniqKey="Klineberg I">I Klineberg</name>
</author>
<author><name sortKey="Goldberg, Pv" uniqKey="Goldberg P">PV Goldberg</name>
</author>
<author><name sortKey="Stevenson Moore, P" uniqKey="Stevenson Moore P">P Stevenson-Moore</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schmitt, A" uniqKey="Schmitt A">A Schmitt</name>
</author>
<author><name sortKey="Zarb, Ga" uniqKey="Zarb G">GA Zarb</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zarb, Ga" uniqKey="Zarb G">GA Zarb</name>
</author>
<author><name sortKey="Schmitt, A" uniqKey="Schmitt A">A Schmitt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wennstrom, Jl" uniqKey="Wennstrom J">JL Wennstrom</name>
</author>
<author><name sortKey="Ekestubbe, A" uniqKey="Ekestubbe A">A Ekestubbe</name>
</author>
<author><name sortKey="Grondahl, K" uniqKey="Grondahl K">K Grondahl</name>
</author>
<author><name sortKey="Karlsson, S" uniqKey="Karlsson S">S Karlsson</name>
</author>
<author><name sortKey="Lindhe, J" uniqKey="Lindhe J">J Lindhe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wennerberg, A" uniqKey="Wennerberg A">A Wennerberg</name>
</author>
<author><name sortKey="Albrektsson, T" uniqKey="Albrektsson T">T Albrektsson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wennerberg, A" uniqKey="Wennerberg A">A Wennerberg</name>
</author>
<author><name sortKey="Albrektsson, T" uniqKey="Albrektsson T">T Albrektsson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meyenberg, Kh" uniqKey="Meyenberg K">KH Meyenberg</name>
</author>
<author><name sortKey="Luthy, H" uniqKey="Luthy H">H Luthy</name>
</author>
<author><name sortKey="Scharer, P" uniqKey="Scharer P">P Scharer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brodbeck, U" uniqKey="Brodbeck U">U Brodbeck</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Glauser, R" uniqKey="Glauser R">R Glauser</name>
</author>
<author><name sortKey="Sailer, I" uniqKey="Sailer I">I Sailer</name>
</author>
<author><name sortKey="Wohlwend, A" uniqKey="Wohlwend A">A Wohlwend</name>
</author>
<author><name sortKey="Studer, S" uniqKey="Studer S">S Studer</name>
</author>
<author><name sortKey="Schibli, M" uniqKey="Schibli M">M Schibli</name>
</author>
<author><name sortKey="Scharer, P" uniqKey="Scharer P">P Scharer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Filser, F" uniqKey="Filser F">F Filser</name>
</author>
<author><name sortKey="Kocher, P" uniqKey="Kocher P">P Kocher</name>
</author>
<author><name sortKey="Weibel, F" uniqKey="Weibel F">F Weibel</name>
</author>
<author><name sortKey="Luthy, H" uniqKey="Luthy H">H Luthy</name>
</author>
<author><name sortKey="Scharer, P" uniqKey="Scharer P">P Scharer</name>
</author>
<author><name sortKey="Gauckler, Lj" uniqKey="Gauckler L">LJ Gauckler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sailer, I" uniqKey="Sailer I">I Sailer</name>
</author>
<author><name sortKey="Feher, A" uniqKey="Feher A">A Feher</name>
</author>
<author><name sortKey="Filser, F" uniqKey="Filser F">F Filser</name>
</author>
<author><name sortKey="Luthy, H" uniqKey="Luthy H">H Luthy</name>
</author>
<author><name sortKey="Gauckler, Lj" uniqKey="Gauckler L">LJ Gauckler</name>
</author>
<author><name sortKey="Scharer, P" uniqKey="Scharer P">P Scharer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Andreiotelli, M" uniqKey="Andreiotelli M">M Andreiotelli</name>
</author>
<author><name sortKey="Wenz, Hj" uniqKey="Wenz H">HJ Wenz</name>
</author>
<author><name sortKey="Kohal, Rj" uniqKey="Kohal R">RJ Kohal</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ozkurt, Z" uniqKey="Ozkurt Z">Z Ozkurt</name>
</author>
<author><name sortKey="Kazazoglu, E" uniqKey="Kazazoglu E">E Kazazoglu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wenz, Hj" uniqKey="Wenz H">HJ Wenz</name>
</author>
<author><name sortKey="Bartsch, J" uniqKey="Bartsch J">J Bartsch</name>
</author>
<author><name sortKey="Wolfart, S" uniqKey="Wolfart S">S Wolfart</name>
</author>
<author><name sortKey="Kern, M" uniqKey="Kern M">M Kern</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Horbett, Ta" uniqKey="Horbett T">TA Horbett</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Albrektsson, T" uniqKey="Albrektsson T">T Albrektsson</name>
</author>
<author><name sortKey="Johansson, C" uniqKey="Johansson C">C Johansson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Anselme, K" uniqKey="Anselme K">K Anselme</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Drosse, I" uniqKey="Drosse I">I Drosse</name>
</author>
<author><name sortKey="Volkmer, E" uniqKey="Volkmer E">E Volkmer</name>
</author>
<author><name sortKey="Capanna, R" uniqKey="Capanna R">R Capanna</name>
</author>
<author><name sortKey="De Biase, P" uniqKey="De Biase P">P De Biase</name>
</author>
<author><name sortKey="Mutschler, W" uniqKey="Mutschler W">W Mutschler</name>
</author>
<author><name sortKey="Schieker, M" uniqKey="Schieker M">M Schieker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vasita, R" uniqKey="Vasita R">R Vasita</name>
</author>
<author><name sortKey="Shanmugam, Ik" uniqKey="Shanmugam I">IK Shanmugam</name>
</author>
<author><name sortKey="Katt, Ds" uniqKey="Katt D">DS Katt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bachle, M" uniqKey="Bachle M">M Bachle</name>
</author>
<author><name sortKey="Butz, F" uniqKey="Butz F">F Butz</name>
</author>
<author><name sortKey="Hubner, U" uniqKey="Hubner U">U Hubner</name>
</author>
<author><name sortKey="Bakalinis, E" uniqKey="Bakalinis E">E Bakalinis</name>
</author>
<author><name sortKey="Kohal, Rj" uniqKey="Kohal R">RJ Kohal</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Luthen, F" uniqKey="Luthen F">F Luthen</name>
</author>
<author><name sortKey="Lange, R" uniqKey="Lange R">R Lange</name>
</author>
<author><name sortKey="Becker, P" uniqKey="Becker P">P Becker</name>
</author>
<author><name sortKey="Rychly, J" uniqKey="Rychly J">J Rychly</name>
</author>
<author><name sortKey="Beck, U" uniqKey="Beck U">U Beck</name>
</author>
<author><name sortKey="Nebe, Jg" uniqKey="Nebe J">JG Nebe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nebe, B" uniqKey="Nebe B">B Nebe</name>
</author>
<author><name sortKey="Forster, C" uniqKey="Forster C">C Forster</name>
</author>
<author><name sortKey="Pommerenke, H" uniqKey="Pommerenke H">H Pommerenke</name>
</author>
<author><name sortKey="Fulda, G" uniqKey="Fulda G">G Fulda</name>
</author>
<author><name sortKey="Behrend, D" uniqKey="Behrend D">D Behrend</name>
</author>
<author><name sortKey="Bernewski, U" uniqKey="Bernewski U">U Bernewski</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yamashita, D" uniqKey="Yamashita D">D Yamashita</name>
</author>
<author><name sortKey="Machigashira, M" uniqKey="Machigashira M">M Machigashira</name>
</author>
<author><name sortKey="Miyamoto, M" uniqKey="Miyamoto M">M Miyamoto</name>
</author>
<author><name sortKey="Takeuchi, H" uniqKey="Takeuchi H">H Takeuchi</name>
</author>
<author><name sortKey="Noguchi, K" uniqKey="Noguchi K">K Noguchi</name>
</author>
<author><name sortKey="Izumi, Y" uniqKey="Izumi Y">Y Izumi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kunz, F" uniqKey="Kunz F">F Kunz</name>
</author>
<author><name sortKey="Bergemann, C" uniqKey="Bergemann C">C Bergemann</name>
</author>
<author><name sortKey="Klinkenberg, Ed" uniqKey="Klinkenberg E">ED Klinkenberg</name>
</author>
<author><name sortKey="Weidmann, A" uniqKey="Weidmann A">A Weidmann</name>
</author>
<author><name sortKey="Lange, R" uniqKey="Lange R">R Lange</name>
</author>
<author><name sortKey="Beck, U" uniqKey="Beck U">U Beck</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Finke, B" uniqKey="Finke B">B Finke</name>
</author>
<author><name sortKey="Luethen, F" uniqKey="Luethen F">F Luethen</name>
</author>
<author><name sortKey="Schroeder, K" uniqKey="Schroeder K">K Schroeder</name>
</author>
<author><name sortKey="Mueller, Pd" uniqKey="Mueller P">PD Mueller</name>
</author>
<author><name sortKey="Bergemann, C" uniqKey="Bergemann C">C Bergemann</name>
</author>
<author><name sortKey="Frant, M" uniqKey="Frant M">M Frant</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Birkholz, H" uniqKey="Birkholz H">H Birkholz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Matschegewski, C" uniqKey="Matschegewski C">C Matschegewski</name>
</author>
<author><name sortKey="Staehlke, S" uniqKey="Staehlke S">S Staehlke</name>
</author>
<author><name sortKey="Birkholz, H" uniqKey="Birkholz H">H Birkholz</name>
</author>
<author><name sortKey="Lange, R" uniqKey="Lange R">R Lange</name>
</author>
<author><name sortKey="Beck, U" uniqKey="Beck U">U Beck</name>
</author>
<author><name sortKey="Engel, U" uniqKey="Engel U">U Engel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nebe, Jg" uniqKey="Nebe J">JG Nebe</name>
</author>
<author><name sortKey="Luethen, F" uniqKey="Luethen F">F Luethen</name>
</author>
<author><name sortKey="Lange, R" uniqKey="Lange R">R Lange</name>
</author>
<author><name sortKey="Beck, U" uniqKey="Beck U">U Beck</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Depprich, R" uniqKey="Depprich R">R Depprich</name>
</author>
<author><name sortKey="Ommerborn, M" uniqKey="Ommerborn M">M Ommerborn</name>
</author>
<author><name sortKey="Zipprich, H" uniqKey="Zipprich H">H Zipprich</name>
</author>
<author><name sortKey="Naujoks, C" uniqKey="Naujoks C">C Naujoks</name>
</author>
<author><name sortKey="Handschel, J" uniqKey="Handschel J">J Handschel</name>
</author>
<author><name sortKey="Wiesmann, Hp" uniqKey="Wiesmann H">HP Wiesmann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wennerberg, A" uniqKey="Wennerberg A">A Wennerberg</name>
</author>
<author><name sortKey="Albrektsson, T" uniqKey="Albrektsson T">T Albrektsson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Uggeri, J" uniqKey="Uggeri J">J Uggeri</name>
</author>
<author><name sortKey="Guizzardi, S" uniqKey="Guizzardi S">S Guizzardi</name>
</author>
<author><name sortKey="Scandroglio, R" uniqKey="Scandroglio R">R Scandroglio</name>
</author>
<author><name sortKey="Gatti, R" uniqKey="Gatti R">R Gatti</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Billiard, J" uniqKey="Billiard J">J Billiard</name>
</author>
<author><name sortKey="Moran, Ra" uniqKey="Moran R">RA Moran</name>
</author>
<author><name sortKey="Whitley, Mz" uniqKey="Whitley M">MZ Whitley</name>
</author>
<author><name sortKey="Chatterjee Kishore, M" uniqKey="Chatterjee Kishore M">M Chatterjee-Kishore</name>
</author>
<author><name sortKey="Gillis, K" uniqKey="Gillis K">K Gillis</name>
</author>
<author><name sortKey="Brown, El" uniqKey="Brown E">EL Brown</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Martin, Jy" uniqKey="Martin J">JY Martin</name>
</author>
<author><name sortKey="Schwartz, Z" uniqKey="Schwartz Z">Z Schwartz</name>
</author>
<author><name sortKey="Hummert, Tw" uniqKey="Hummert T">TW Hummert</name>
</author>
<author><name sortKey="Schraub, Dm" uniqKey="Schraub D">DM Schraub</name>
</author>
<author><name sortKey="Simpson, J" uniqKey="Simpson J">J Simpson</name>
</author>
<author><name sortKey="Lankford, J" uniqKey="Lankford J">J Lankford</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schneider, Gb" uniqKey="Schneider G">GB Schneider</name>
</author>
<author><name sortKey="Perinpanayagam, H" uniqKey="Perinpanayagam H">H Perinpanayagam</name>
</author>
<author><name sortKey="Clegg, M" uniqKey="Clegg M">M Clegg</name>
</author>
<author><name sortKey="Zaharias, R" uniqKey="Zaharias R">R Zaharias</name>
</author>
<author><name sortKey="Seabold, D" uniqKey="Seabold D">D Seabold</name>
</author>
<author><name sortKey="Keller, J" uniqKey="Keller J">J Keller</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Masaki, C" uniqKey="Masaki C">C Masaki</name>
</author>
<author><name sortKey="Schneider, Gb" uniqKey="Schneider G">GB Schneider</name>
</author>
<author><name sortKey="Zaharias, R" uniqKey="Zaharias R">R Zaharias</name>
</author>
<author><name sortKey="Seabold, D" uniqKey="Seabold D">D Seabold</name>
</author>
<author><name sortKey="Stanford, C" uniqKey="Stanford C">C Stanford</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Carinci, F" uniqKey="Carinci F">F Carinci</name>
</author>
<author><name sortKey="Pezzetti, F" uniqKey="Pezzetti F">F Pezzetti</name>
</author>
<author><name sortKey="Volinia, S" uniqKey="Volinia S">S Volinia</name>
</author>
<author><name sortKey="Francioso, F" uniqKey="Francioso F">F Francioso</name>
</author>
<author><name sortKey="Arcelli, D" uniqKey="Arcelli D">D Arcelli</name>
</author>
<author><name sortKey="Farina, E" uniqKey="Farina E">E Farina</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kohal, Rj" uniqKey="Kohal R">RJ Kohal</name>
</author>
<author><name sortKey="Bachle, M" uniqKey="Bachle M">M Bachle</name>
</author>
<author><name sortKey="Att, W" uniqKey="Att W">W Att</name>
</author>
<author><name sortKey="Chaar, S" uniqKey="Chaar S">S Chaar</name>
</author>
<author><name sortKey="Altmann, B" uniqKey="Altmann B">B Altmann</name>
</author>
<author><name sortKey="Renz, A" uniqKey="Renz A">A Renz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Higuchi, C" uniqKey="Higuchi C">C Higuchi</name>
</author>
<author><name sortKey="Nakamura, N" uniqKey="Nakamura N">N Nakamura</name>
</author>
<author><name sortKey="Yoshikawa, H" uniqKey="Yoshikawa H">H Yoshikawa</name>
</author>
<author><name sortKey="Itoh, K" uniqKey="Itoh K">K Itoh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Altmann, B" uniqKey="Altmann B">B Altmann</name>
</author>
<author><name sortKey="Kohal, Rj" uniqKey="Kohal R">RJ Kohal</name>
</author>
<author><name sortKey="Steinberg, T" uniqKey="Steinberg T">T Steinberg</name>
</author>
<author><name sortKey="Tomakidi, P" uniqKey="Tomakidi P">P Tomakidi</name>
</author>
<author><name sortKey="Bachle Haas, M" uniqKey="Bachle Haas M">M Bachle-Haas</name>
</author>
<author><name sortKey="Wennerberg, A" uniqKey="Wennerberg A">A Wennerberg</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Mater Sci Mater Med</journal-id>
<journal-id journal-id-type="iso-abbrev">J Mater Sci Mater Med</journal-id>
<journal-title-group><journal-title>Journal of Materials Science. Materials in Medicine</journal-title>
</journal-title-group>
<issn pub-type="ppub">0957-4530</issn>
<issn pub-type="epub">1573-4838</issn>
<publisher><publisher-name>Springer US</publisher-name>
<publisher-loc>New York</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">25578704</article-id>
<article-id pub-id-type="pmc">4289972</article-id>
<article-id pub-id-type="publisher-id">5350</article-id>
<article-id pub-id-type="doi">10.1007/s10856-014-5350-x</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Tissue Engineering Constructs and Cell Substrates</subject>
</subj-group>
</article-categories>
<title-group><article-title>Microstructured zirconia surfaces modulate osteogenic marker genes in human primary osteoblasts</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Bergemann</surname>
<given-names>Claudia</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Duske</surname>
<given-names>Kathrin</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Nebe</surname>
<given-names>J. Barbara</given-names>
</name>
<address><phone>+49 381 494 7771</phone>
<email>barbara.nebe@med.uni-rostock.de</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Schöne</surname>
<given-names>André</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Bulnheim</surname>
<given-names>Ulrike</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Seitz</surname>
<given-names>Hermann</given-names>
</name>
<xref ref-type="aff" rid="Aff4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Fischer</surname>
<given-names>Jens</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
<xref ref-type="aff" rid="Aff3">3</xref>
</contrib>
<aff id="Aff1"><label>1</label>
Department of Cell Biology, University Medical Center Rostock, Schillingallee 69, 18057 Rostock, Germany</aff>
<aff id="Aff2"><label>2</label>
VITA Zahnfabrik, H. Rauter GmbH & Co.KG, Spitalgasse 3, PO Box 1338, 79713 Bad Säckingen, Germany</aff>
<aff id="Aff3"><label>3</label>
Institute for Dental Materials and Engineering, University of Basel, Hebelstrasse 3, 4056 Basel, Switzerland</aff>
<aff id="Aff4"><label>4</label>
Fluid Technology and Microfluidics, University of Rostock, Justus-von-Liebig Weg 6, 18057 Rostock, Germany</aff>
</contrib-group>
<pub-date pub-type="epub"><day>13</day>
<month>1</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>13</day>
<month>1</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="ppub"><year>2015</year>
</pub-date>
<volume>26</volume>
<issue>1</issue>
<elocation-id>26</elocation-id>
<history><date date-type="received"><day>1</day>
<month>5</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>4</day>
<month>8</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>© The Author(s) 2014</copyright-statement>
<license license-type="OpenAccess"><license-p><bold>Open Access</bold>
This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.</license-p>
</license>
</permissions>
<abstract id="Abs1"><p>In dentistry, zirconia has been used since the early 1990s for endodontic posts, more recently for implant abutments and frameworks for fixed dental prostheses. Zirconia is biocompatible and mechanically strong enough to serve as implant material for oral implants. Although several zirconia implant systems are available, currently the scientific and clinical data for zirconia implants are not sufficient to recommend them for routine clinical use. Here the influence of microstructured yttria-stabilized zirconia (YZ) on human primary osteoblast (HOB) behavior was determined. YZ surfaces were treated by sandblasting (YZ-S), acid etching (YZ-SE) and additionally heat treatment (YZ-SEH). Morphological changes of HOB were determined by scanning electron microscopy. Actin cytoskeleton was investigated by laser scanning microscopy and analyzed by novel actin quantification software. Differentiation of HOB was determined by real time RT-PCR. Improved mechanical interlocking of primary HOB into the porous microstructure of the acid etched and additionally heat treated YZ-surfaces correlates with drastically increased osteocalcin (OCN) gene expression. In particular, OCN was considerably elevated in primary HOB after 3 days on YZ-SE (13-fold) as well as YZ-SEH (12-fold) surfaces. Shorter actin filaments without any favored orientation on YZ-SE and YZ-SEH surfaces are associated with higher roughness (R<sub>a</sub>
) values. Topographically modified yttria-stabilized zirconia is a likely material for dental implants with cell stimulating properties achieving or actually exceeding those of titanium.
</p>
</abstract>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© Springer Science+Business Media New York 2015</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
</record>
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