Pyrosequencing of supra- and subgingival biofilms from inflamed peri-implant and periodontal sites
Identifieur interne : 000B86 ( Pmc/Curation ); précédent : 000B85; suivant : 000B87Pyrosequencing of supra- and subgingival biofilms from inflamed peri-implant and periodontal sites
Auteurs : Simone Schaumann [Allemagne] ; Ingmar Staufenbiel [Allemagne] ; Ralph Scherer [Allemagne] ; Markus Schilhabel [Allemagne] ; Andreas Winkel [Allemagne] ; Sascha Nico Stumpp [Allemagne] ; Jörg Eberhard [Allemagne] ; Meike Stiesch [Allemagne]Source :
- BMC Oral Health [ 1472-6831 ] ; 2014.
Abstract
To investigate the microbial composition of biofilms at inflamed peri-implant and periodontal tissues in the same subject, using 16S rRNA sequencing.
Supra- and submucosal, and supra- and subgingival plaque samples were collected from 7 subjects suffering from diseased peri-implant and periodontal tissues. Bacterial DNA was isolated and 16S rRNA genes were amplified, sequenced and aligned for the identification of bacterial genera.
43734 chimera-depleted, denoised sequences were identified, corresponding to 1 phylum, 8 classes, 10 orders, 44 families and 150 genera. The most abundant families or genera found in supramucosal or supragingival plaque were
Diseased peri-implant and periodontal tissues in the same subject share similiar bacterial genera and based on the analysis of taxa on a genus level biofilm compositions may not account for the potentially distinct pathologies at implants or teeth.
The online version of this article (doi:10.1186/1472-6831-14-157) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/1472-6831-14-157
PubMed: 25518856
PubMed Central: 4298060
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Pyrosequencing of supra- and subgingival biofilms from inflamed peri-implant and periodontal sites</title><author><name sortKey="Schaumann, Simone" sort="Schaumann, Simone" uniqKey="Schaumann S" first="Simone" last="Schaumann">Simone Schaumann</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Staufenbiel, Ingmar" sort="Staufenbiel, Ingmar" uniqKey="Staufenbiel I" first="Ingmar" last="Staufenbiel">Ingmar Staufenbiel</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Hannover Medical School, Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Hannover Medical School, Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Scherer, Ralph" sort="Scherer, Ralph" uniqKey="Scherer R" first="Ralph" last="Scherer">Ralph Scherer</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Institute for Biometry, Hannover Medical School, Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Biometry, Hannover Medical School, Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Schilhabel, Markus" sort="Schilhabel, Markus" uniqKey="Schilhabel M" first="Markus" last="Schilhabel">Markus Schilhabel</name><affiliation wicri:level="1"><nlm:aff id="Aff4">Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel, Kiel, Germany</nlm:aff><country 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Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Eberhard, Jorg" sort="Eberhard, Jorg" uniqKey="Eberhard J" first="Jörg" last="Eberhard">Jörg Eberhard</name><affiliation wicri:level="1"><nlm:aff id="Aff5">Peri-implant and Oral Infections, Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Peri-implant and Oral Infections, Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Stiesch, Meike" sort="Stiesch, Meike" uniqKey="Stiesch M" first="Meike" last="Stiesch">Meike Stiesch</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25518856</idno><idno type="pmc">4298060</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4298060</idno><idno type="RBID">PMC:4298060</idno><idno type="doi">10.1186/1472-6831-14-157</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000B86</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000B86</idno><idno type="wicri:Area/Pmc/Curation">000B86</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000B86</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Pyrosequencing of supra- and subgingival biofilms from inflamed peri-implant and periodontal sites</title><author><name sortKey="Schaumann, Simone" sort="Schaumann, Simone" uniqKey="Schaumann S" first="Simone" last="Schaumann">Simone Schaumann</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Staufenbiel, Ingmar" sort="Staufenbiel, Ingmar" uniqKey="Staufenbiel I" first="Ingmar" last="Staufenbiel">Ingmar Staufenbiel</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Hannover Medical School, Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Hannover Medical School, Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Scherer, Ralph" sort="Scherer, Ralph" uniqKey="Scherer R" first="Ralph" last="Scherer">Ralph Scherer</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Institute for Biometry, Hannover Medical School, Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Biometry, Hannover Medical School, Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Schilhabel, Markus" sort="Schilhabel, Markus" uniqKey="Schilhabel M" first="Markus" last="Schilhabel">Markus Schilhabel</name><affiliation wicri:level="1"><nlm:aff id="Aff4">Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel, Kiel, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel, Kiel</wicri:regionArea></affiliation></author><author><name sortKey="Winkel, Andreas" sort="Winkel, Andreas" uniqKey="Winkel A" first="Andreas" last="Winkel">Andreas Winkel</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Stumpp, Sascha Nico" sort="Stumpp, Sascha Nico" uniqKey="Stumpp S" first="Sascha Nico" last="Stumpp">Sascha Nico Stumpp</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Eberhard, Jorg" sort="Eberhard, Jorg" uniqKey="Eberhard J" first="Jörg" last="Eberhard">Jörg Eberhard</name><affiliation wicri:level="1"><nlm:aff id="Aff5">Peri-implant and Oral Infections, Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Peri-implant and Oral Infections, Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover</wicri:regionArea></affiliation></author><author><name sortKey="Stiesch, Meike" sort="Stiesch, Meike" uniqKey="Stiesch M" first="Meike" last="Stiesch">Meike Stiesch</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover</wicri:regionArea></affiliation></author></analytic><series><title level="j">BMC Oral Health</title><idno type="eISSN">1472-6831</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>To investigate the microbial composition of biofilms at inflamed peri-implant and periodontal tissues in the same subject, using 16S rRNA sequencing.</p></sec><sec><title>Methods</title><p>Supra- and submucosal, and supra- and subgingival plaque samples were collected from 7 subjects suffering from diseased peri-implant and periodontal tissues. Bacterial DNA was isolated and 16S rRNA genes were amplified, sequenced and aligned for the identification of bacterial genera.</p></sec><sec><title>Results</title><p>43734 chimera-depleted, denoised sequences were identified, corresponding to 1 phylum, 8 classes, 10 orders, 44 families and 150 genera. The most abundant families or genera found in supramucosal or supragingival plaque were <italic>Streptoccocaceae, Rothia</italic> and <italic>Porphyromonas.</italic> In submucosal plaque, the most abundant family or genera found were <italic>Rothia, Streptococcaceae</italic> and <italic>Porphyromonas</italic> on implants. The most abundant subgingival bacteria on teeth were <italic>Prevotella, Streptococcaceae,</italic> and <italic>TG5.</italic> The number of sequences found for the genera <italic>Tannerella</italic> and <italic>Aggregatibacter</italic> on implants differed significantly between supra- and submucosal locations before multiple testing. The analyses demonstrated no significant differences between microbiomes on implants and teeth in supra- or submucosal and supra- or subgingival biofilms.</p></sec><sec><title>Conclusion</title><p>Diseased peri-implant and periodontal tissues in the same subject share similiar bacterial genera and based on the analysis of taxa on a genus level biofilm compositions may not account for the potentially distinct pathologies at implants or teeth.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1472-6831-14-157) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Mombelli, A" uniqKey="Mombelli A">A Mombelli</name></author><author><name sortKey="Muller, N" uniqKey="Muller N">N Muller</name></author><author><name sortKey="Cionca, N" uniqKey="Cionca N">N 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article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Oral Health</journal-id><journal-id journal-id-type="iso-abbrev">BMC Oral Health</journal-id><journal-title-group><journal-title>BMC Oral Health</journal-title></journal-title-group><issn pub-type="epub">1472-6831</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25518856</article-id><article-id pub-id-type="pmc">4298060</article-id><article-id pub-id-type="publisher-id">486</article-id><article-id pub-id-type="doi">10.1186/1472-6831-14-157</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Pyrosequencing of supra- and subgingival biofilms from inflamed peri-implant and periodontal sites</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Schaumann</surname><given-names>Simone</given-names></name><address><email>schaumann.simone@mh-hannover.de</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Staufenbiel</surname><given-names>Ingmar</given-names></name><address><email>staufenbiel.ingmar@mh-hannover.de</email></address><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author"><name><surname>Scherer</surname><given-names>Ralph</given-names></name><address><email>scherer.ralph@mh-hannover.de</email></address><xref ref-type="aff" rid="Aff3"></xref></contrib><contrib contrib-type="author"><name><surname>Schilhabel</surname><given-names>Markus</given-names></name><address><email>m.schilhabel@ikmb.uni-kiel.de</email></address><xref ref-type="aff" rid="Aff4"></xref></contrib><contrib contrib-type="author"><name><surname>Winkel</surname><given-names>Andreas</given-names></name><address><email>winkel.andreas@mh-hannover.de</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Stumpp</surname><given-names>Sascha Nico</given-names></name><address><email>stumpp.nico@mh-hannover.de</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author" corresp="yes" equal-contrib="yes"><name><surname>Eberhard</surname><given-names>Jörg</given-names></name><address><email>Eberhard.joerg@mh-hannover.de</email></address><xref ref-type="aff" rid="Aff5"></xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Stiesch</surname><given-names>Meike</given-names></name><address><email>stiesch.meike@mh-hannover.de</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><aff id="Aff1"><label></label>Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany</aff><aff id="Aff2"><label></label>Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Hannover Medical School, Hannover, Germany</aff><aff id="Aff3"><label></label>Institute for Biometry, Hannover Medical School, Hannover, Germany</aff><aff id="Aff4"><label></label>Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel, Kiel, Germany</aff><aff id="Aff5"><label></label>Peri-implant and Oral Infections, Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany</aff></contrib-group><pub-date pub-type="epub"><day>17</day><month>12</month><year>2014</year></pub-date><pub-date pub-type="pmc-release"><day>17</day><month>12</month><year>2014</year></pub-date><pub-date pub-type="collection"><year>2014</year></pub-date><volume>14</volume><elocation-id>157</elocation-id><history><date date-type="received"><day>28</day><month>8</month><year>2014</year></date><date date-type="accepted"><day>15</day><month>12</month><year>2014</year></date></history><permissions><copyright-statement>© Schaumann et al.; licensee BioMed Central. 2014</copyright-statement><license license-type="open-access"><license-p>This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0">http://creativecommons.org/licenses/by/4.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>) applies to the data made available in this article, unless otherwise stated.</license-p></license></permissions><abstract id="Abs1"><sec><title>Background</title><p>To investigate the microbial composition of biofilms at inflamed peri-implant and periodontal tissues in the same subject, using 16S rRNA sequencing.</p></sec><sec><title>Methods</title><p>Supra- and submucosal, and supra- and subgingival plaque samples were collected from 7 subjects suffering from diseased peri-implant and periodontal tissues. Bacterial DNA was isolated and 16S rRNA genes were amplified, sequenced and aligned for the identification of bacterial genera.</p></sec><sec><title>Results</title><p>43734 chimera-depleted, denoised sequences were identified, corresponding to 1 phylum, 8 classes, 10 orders, 44 families and 150 genera. The most abundant families or genera found in supramucosal or supragingival plaque were <italic>Streptoccocaceae, Rothia</italic> and <italic>Porphyromonas.</italic> In submucosal plaque, the most abundant family or genera found were <italic>Rothia, Streptococcaceae</italic> and <italic>Porphyromonas</italic> on implants. The most abundant subgingival bacteria on teeth were <italic>Prevotella, Streptococcaceae,</italic> and <italic>TG5.</italic> The number of sequences found for the genera <italic>Tannerella</italic> and <italic>Aggregatibacter</italic> on implants differed significantly between supra- and submucosal locations before multiple testing. The analyses demonstrated no significant differences between microbiomes on implants and teeth in supra- or submucosal and supra- or subgingival biofilms.</p></sec><sec><title>Conclusion</title><p>Diseased peri-implant and periodontal tissues in the same subject share similiar bacterial genera and based on the analysis of taxa on a genus level biofilm compositions may not account for the potentially distinct pathologies at implants or teeth.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1472-6831-14-157) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Deep-sequencing</kwd><kwd>16S rRNA sequencing</kwd><kwd>Diseased peri-implant tissues</kwd><kwd>Diseased periodontal tissues</kwd><kwd>Supragingival plaque</kwd><kwd>Subgingival plaque</kwd><kwd>Biofilm</kwd><kwd>Microbiology</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2014</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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