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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Assessment of gingival thickness with regards to age, gender and arch location</title><author><name sortKey="Kolte, Rajashri" sort="Kolte, Rajashri" uniqKey="Kolte R" first="Rajashri" last="Kolte">Rajashri Kolte</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Kolte, Abhay" sort="Kolte, Abhay" uniqKey="Kolte A" first="Abhay" last="Kolte">Abhay Kolte</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Mahajan, Aaditi" sort="Mahajan, Aaditi" uniqKey="Mahajan A" first="Aaditi" last="Mahajan">Aaditi Mahajan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25210263</idno><idno type="pmc">4158590</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4158590</idno><idno type="RBID">PMC:4158590</idno><idno type="doi">10.4103/0972-124X.138699</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">003178</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003178</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Assessment of gingival thickness with regards to age, gender and arch location</title><author><name sortKey="Kolte, Rajashri" sort="Kolte, Rajashri" uniqKey="Kolte R" first="Rajashri" last="Kolte">Rajashri Kolte</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Kolte, Abhay" sort="Kolte, Abhay" uniqKey="Kolte A" first="Abhay" last="Kolte">Abhay Kolte</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Mahajan, Aaditi" sort="Mahajan, Aaditi" uniqKey="Mahajan A" first="Aaditi" last="Mahajan">Aaditi Mahajan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Indian Society of Periodontology</title><idno type="ISSN">0972-124X</idno><idno type="eISSN">0975-1580</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="st1"><title>Background:</title><p>There is a considerable intra and inter-individual variation in both width and thickness of the facial gingiva. As the attached gingiva is an important anatomic and functional landmark in the periodontium, the identification of gingival biotype is important in clinical practice since differences in gingival and osseous architecture have been shown to exhibit a significant impact on the outcome of restorative therapy. Hence, the aim of this study was to determine the variation in width and thickness of facial gingiva in the anterior segment with respect to age, gender and dental arch location.</p></sec><sec id="st2"><title>Materials and Methods:</title><p>120 subjects were divided into three age groups: The younger age group (16-24 years), the middle age group (25-39 years) and the older age group (>40 years) with 20 males and 20 females in each group. The width of the gingiva was assessed by William's graduated probe and the thickness was determined using transgingival probing in the maxillary and mandibular anterior segment.</p></sec><sec id="st3"><title>Results:</title><p>It was observed that the younger age group had significantly thicker gingiva but less width than that of the older age group. The gingiva was found to be thinner and with less width in females than males. The mandibular arch had thicker gingiva with less width compared to the maxillary arch.</p></sec><sec id="st4"><title>Conclusion:</title><p>In the present study, we concluded that gingival thickness and width varies with age, gender and dental arch location.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Seibert, J" uniqKey="Seibert J">J Seibert</name></author><author><name sortKey="Lindhe, J" uniqKey="Lindhe J">J Lindhe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Olsson, M" uniqKey="Olsson M">M Olsson</name></author><author><name sortKey="Lindhe, J" uniqKey="Lindhe J">J Lindhe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muller, Hp" uniqKey="Muller H">HP Müller</name></author><author><name sortKey="Eger, T" uniqKey="Eger T">T Eger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pontoriero, R" uniqKey="Pontoriero R">R Pontoriero</name></author><author><name sortKey="Carnevale, G" uniqKey="Carnevale G">G Carnevale</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Eger, T" uniqKey="Eger T">T Eger</name></author><author><name sortKey="Muller, Hp" uniqKey="Muller H">HP Müller</name></author><author><name sortKey="Heinecke, A" uniqKey="Heinecke A">A Heinecke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muller, Hp" uniqKey="Muller H">HP Müller</name></author><author><name sortKey="Schaller, N" uniqKey="Schaller N">N Schaller</name></author><author><name sortKey="Eger, T" uniqKey="Eger T">T Eger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barriviera, M" uniqKey="Barriviera M">M Barriviera</name></author><author><name sortKey="Duarte, Wr" uniqKey="Duarte W">WR Duarte</name></author><author><name sortKey="Janua Rio, Al" uniqKey="Janua Rio A">AL Janua×rio</name></author><author><name sortKey="Faber, J" uniqKey="Faber J">J Faber</name></author><author><name sortKey="Bezerra, Ac" uniqKey="Bezerra A">AC Bezerra</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Studer, Sp" uniqKey="Studer S">SP Studer</name></author><author><name sortKey="Allen, Ep" uniqKey="Allen E">EP Allen</name></author><author><name sortKey="Rees, Tc" uniqKey="Rees T">TC Rees</name></author><author><name sortKey="Kouba, A" uniqKey="Kouba A">A Kouba</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Waraaswapati, N" uniqKey="Waraaswapati N">N Waraaswapati</name></author><author><name sortKey="Pitiphat, W" uniqKey="Pitiphat W">W Pitiphat</name></author><author><name sortKey="Chandrapho, N" uniqKey="Chandrapho N">N Chandrapho</name></author><author><name sortKey="Rattanayatikul, C" uniqKey="Rattanayatikul C">C Rattanayatikul</name></author><author><name sortKey="Karimbux, N" uniqKey="Karimbux N">N Karimbux</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vandana, Kl" uniqKey="Vandana K">KL Vandana</name></author><author><name sortKey="Savitha, B" uniqKey="Savitha B">B Savitha</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muller, Hp" uniqKey="Muller H">HP Muller</name></author><author><name sortKey="Schaller, N" uniqKey="Schaller N">N Schaller</name></author><author><name sortKey="Eger, T" uniqKey="Eger T">T Eger</name></author><author><name sortKey="Heinecke, A" uniqKey="Heinecke A">A Heinecke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, Je" uniqKey="Song J">JE Song</name></author><author><name sortKey="Um, Yj" uniqKey="Um Y">YJ Um</name></author><author><name sortKey="Kim, Cs" uniqKey="Kim C">CS Kim</name></author><author><name sortKey="Choi, Sh" uniqKey="Choi S">SH Choi</name></author><author><name sortKey="Cho, Ks" uniqKey="Cho K">KS Cho</name></author><author><name sortKey="Kim, Ck" uniqKey="Kim C">CK Kim</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Indian Soc Periodontol</journal-id><journal-id journal-id-type="iso-abbrev">J Indian Soc Periodontol</journal-id><journal-id journal-id-type="publisher-id">JISP</journal-id><journal-title-group><journal-title>Journal of Indian Society of Periodontology</journal-title></journal-title-group><issn pub-type="ppub">0972-124X</issn><issn pub-type="epub">0975-1580</issn><publisher><publisher-name>Medknow Publications & Media Pvt Ltd</publisher-name><publisher-loc>India</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25210263</article-id><article-id pub-id-type="pmc">4158590</article-id><article-id pub-id-type="publisher-id">JISP-18-478</article-id><article-id pub-id-type="doi">10.4103/0972-124X.138699</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Assessment of gingival thickness with regards to age, gender and arch location</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Kolte</surname><given-names>Rajashri</given-names></name><xref ref-type="aff" rid="aff1"></xref><xref ref-type="corresp" rid="cor1"></xref></contrib><contrib contrib-type="author"><name><surname>Kolte</surname><given-names>Abhay</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Mahajan</surname><given-names>Aaditi</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib></contrib-group><aff id="aff1"><italic>Department of Periodontics, VSPM Dental College and Research Centre, Nagpur, Maharashtra, India</italic></aff><author-notes><corresp id="cor1"><bold>Address for correspondence:</bold> Dr. Rajashri Kolte, B-301, Poonam Heights, Pande Lay Out, Khamla Road, Nagpur - 440 025, Maharashtra, India. E-mail: <email xlink:href="drrajashrikolte@gmail.com">drrajashrikolte@gmail.com</email></corresp></author-notes><pub-date pub-type="ppub"><season>Jul-Aug</season><year>2014</year></pub-date><volume>18</volume><issue>4</issue><fpage>478</fpage><lpage>481</lpage><history><date date-type="received"><day>24</day><month>5</month><year>2013</year></date><date date-type="accepted"><day>03</day><month>12</month><year>2013</year></date></history><permissions><copyright-statement>Copyright: © Journal of Indian Society of Periodontology</copyright-statement><copyright-year>2014</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><sec id="st1"><title>Background:</title><p>There is a considerable intra and inter-individual variation in both width and thickness of the facial gingiva. As the attached gingiva is an important anatomic and functional landmark in the periodontium, the identification of gingival biotype is important in clinical practice since differences in gingival and osseous architecture have been shown to exhibit a significant impact on the outcome of restorative therapy. Hence, the aim of this study was to determine the variation in width and thickness of facial gingiva in the anterior segment with respect to age, gender and dental arch location.</p></sec><sec id="st2"><title>Materials and Methods:</title><p>120 subjects were divided into three age groups: The younger age group (16-24 years), the middle age group (25-39 years) and the older age group (>40 years) with 20 males and 20 females in each group. The width of the gingiva was assessed by William's graduated probe and the thickness was determined using transgingival probing in the maxillary and mandibular anterior segment.</p></sec><sec id="st3"><title>Results:</title><p>It was observed that the younger age group had significantly thicker gingiva but less width than that of the older age group. The gingiva was found to be thinner and with less width in females than males. The mandibular arch had thicker gingiva with less width compared to the maxillary arch.</p></sec><sec id="st4"><title>Conclusion:</title><p>In the present study, we concluded that gingival thickness and width varies with age, gender and dental arch location.</p></sec></abstract><kwd-group><kwd>Attached gingiva</kwd><kwd>age</kwd><kwd>gingival thickness</kwd><kwd>gender</kwd><kwd>transgingival probing</kwd></kwd-group></article-meta></front><body><sec sec-type="intro" id="sec1-1"><title>INTRODUCTION</title><p>Attached gingiva is one of the most important anatomic and functional landmarks in the periodontium. It is defined as the distance between the mucogingival junction and the projection on the external surface of the bottom of gingival sulcus or the periodontal pocket. It is not synonymous with keratinized gingiva because the latter also includes the free gingival margin. An adequate width of the attached gingiva helps in maintaining esthetics and enhanced plaque control. Restoring an adequate width of attached gingiva is a part of the periodontal plastic and esthetic surgery. Sufficient thickness of the attached gingiva is desired as a thin and delicate gingival margin may lead to recession after trauma, surgical or inflammatory injuries. It may also aggravate the existing recession post operatively.</p><p>Normally, there is a considerable intra and inter-individual variation in both width[<xref rid="ref1" ref-type="bibr">1</xref>] and thickness of the facial gingiva,[<xref rid="ref2" ref-type="bibr">2</xref>] a fact that gives rise to the assumption that different gingival phenotypes might exist in any adult population. Müller and Eger in 1997[<xref rid="ref3" ref-type="bibr">3</xref>] coined the term ‘gingival or periodontal phenotype’ to address a common clinical observation of great variation in thickness and width of facial keratinized tissue. The identification of gingival biotype may be important in clinical practice since differences in gingival and osseous architecture have been shown to exhibit a significant impact on the outcome of restorative therapy. In natural teeth Pontoriero and Carnevale (2001)[<xref rid="ref4" ref-type="bibr">4</xref>] showed that more soft tissue regained following crown lengthening procedures in patients with a so-called thick flat biotype, than in those with a ‘thin scalloped biotype’. This observation is in line with a higher prevalence of gingival recession in the latter as reported by Olsson and Lindhe (1991).[<xref rid="ref2" ref-type="bibr">2</xref>] Also, at implant restorations, the gingival biotype has been described as one of the key elements decisive for a successful treatment outcome. Various invasive and non-invasive methods can be used to assess the masticatory mucosa such as conventional histology on cadaver jaws, injection needle, transgingival probing, histologic sections, cephalometric radiographs and cone-beam computerized tomography (CBCT).</p><p>Most of the studies conducted earlier were either carried out on edentulous patients or they estimated only the thickness of the palatal masticatory mucosa. The palatal masticatory mucosa has a less bearing as far as aesthetics or gingival recession is concerned. Also, it was felt that literature needs to be augmented with regards to the understandings of thickness and width of gingiva in the anterior maxillary and mandibular sextants, which bear the maximum brunt of the gingival recession and have greater esthetic relevance.[<xref rid="ref2" ref-type="bibr">2</xref><xref rid="ref3" ref-type="bibr">3</xref>] Hence, the aim of this study was to determine the variation in width and thickness of facial gingiva in the anterior segment with respect to age, gender and dental arch location.</p></sec><sec sec-type="materials|methods" id="sec1-2"><title>MATERIALS AND METHODS</title><p>A total of 120 systemically healthy adults participated in this study. Patients were selected randomly. Power analysis was performed to determine the sample size. As difference between width of attached gingiva of maxillary and mandibular arch was 1.15 with standard deviation of 1.21; α error (5%); Power (1-β) 90%, hence, minimum sample size required was 40. Prior to the study, purpose and the design of the study were explained to the patient and informed consent was obtained. The study was approved by the Institutional Ethics Committee of VSPM Dental College and Research Centre, Nagpur, India. Periodontally healthy subjects with no loss of attachment and patients having all anterior teeth in the maxillary and mandibular arch and those who do not require special health care for daily activities were included in this study. Exclusion criteria were hospitalized patients, pregnant and lactating females, patients with gingival recession in the anterior teeth and extensive restorations and those under medication affecting the periodontium such as cyclosporin A, calcium channel blockers, phenytoin. The younger age group included subjects with 16-24 years of age, the middle age group comprised subjects between 25 and 39 years of age and the older age group having subjects of 40 years and above age. Each group had a population comprising of 20 males and 20 females.</p><p>In the first visit, Plaque Index (Turesky- Gilmore-Glickman modification of Quigley Hein plaque index 1970), Papillary Bleeding Index (Muhlemann and Son 1977) and Probing depth using a William's graduated periodontal probe were recorded followed by scaling and polishing.</p><p>After anesthetizing the facial gingiva with a Lidocaine spray (LOX 10%), the width of the attached gingiva was determined by stretching the lip or cheek and subtracting the sulcus or pocket depth from the total width of the attached gingiva that is from the marginal gingiva to mucogingival junction. This estimation was done using a periodontal probe fitted with an endodontic rubber stopper and the measurements were recorded with the help of a steel ruler calibrated at 1 millimeter. Gingival thickness was assessed midbuccally halfway between the mucogingival junction and the free gingival groove in the attached gingiva using an endodontic spreader fitted with a rubber stopper and measured on the ruler [<xref ref-type="fig" rid="F1">Figure 1</xref>]. Measurements were not rounded off to the nearest millimeter. Both the width and the thickness of the attached gingiva were recorded for 6 maxillary and 6 mandibular anterior teeth. Measurement errors were minimized by allowing only one person (AM) to perform the measurements three times for each area and the most frequently measured and recorded readings were selected as the final measurement.</p><fig id="F1" position="float"><label>Figure 1</label><caption><p>Measurement of thickness of gingiva using an endodontic spreader</p></caption><graphic xlink:href="JISP-18-478-g001"></graphic></fig><sec id="sec2-1"><title>Statistical analysis</title><p>Mean width and thickness of gingiva were compared across different age groups by performing one-way analysis of variance (ANOVA). The unpaired <italic>t</italic>-test was used to compare mean width and thickness of gingiva between male and female subjects and also to compare between maxillary and mandibular arches. Correlation coefficient was used to assess magnitude and nature of correlation between width and thickness of gingiva. <italic>P</italic> < 0.05 was considered as statistically significant. Data was analyzed using statistical software STATA version 10.0. Data had normal distribution to use the <italic>t</italic>-test.</p></sec></sec><sec sec-type="results" id="sec1-3"><title>RESULTS</title><p>The mean width of attached gingiva in the maxillary arch and the mandibular arch for 16-24 years age group was 2.66 (±0.52) mm and 1.89 (±0.6) mm, respectively; for the middle age group (25-39 years) it was 2.82 (±0.54) and 2.11 (±0.53) mm, respectively and for the higher age group (>40 years) it was 3.71 (±0.50) mm and 3.04 (±0.48) mm, respectively. The width of the attached gingiva significantly increased with age for both the arches [<xref ref-type="table" rid="T1">Table 1</xref>].</p><table-wrap id="T1" position="float"><label>Table 1</label><caption><p>Mean width of attached gingiva in different age groups separately for maxillary and mandibular arch</p></caption><graphic xlink:href="JISP-18-478-g002"></graphic></table-wrap><p>The mean width of attached gingiva in the maxillary arch and the mandibular arch in males was 3.03 (±0.56) mm and 3.10 (±0.61) mm, respectively; and for females it was 2.33 (±0.71) mm and 2.37 (±0.78) mm, respectively. The width of the attached gingiva was higher in males compared to females but not statistically significant [<xref ref-type="table" rid="T2">Table 2</xref>].</p><table-wrap id="T2" position="float"><label>Table 2</label><caption><p>Mean width of attached gingiva in different sex groups separately for maxillary and mandibular arch</p></caption><graphic xlink:href="JISP-18-478-g003"></graphic></table-wrap><p>The mean width of the attached gingiva was 3.06 (±0.69) mm and 2.35 (±0.74) mm for the maxillary arch and mandibular arch, respectively. This shows that it was significantly higher in maxilla compared to mandible (<italic>P</italic> < 0.0001).</p><p>The mean thickness of attached gingiva in the maxillary arch and the mandibular arch for 16-24 years age group was 1.60 (±0.43) mm and 1.70 (±0.59) mm respectively; for the middle age group (25-39 years) it was 0.86 (±0.26) mm and 0.91 (±0.25) mm respectively and for the higher age group (>40 years) it was 0.66 (±0.25) mm and 0.75 (±0.71) mm, respectively. The thickness of the attached gingiva significantly decreased with age for both the arches (<italic>P</italic> < 0.0001).</p><p>The mean thickness of attached gingiva in the maxillary arch and the mandibular arch in males was 1.04 (±0.59) mm and 1.26 (±0.67) mm, respectively, whereas in females it was 0.91 (±0.48) mm and 1.17 (±0.71) mm, respectively. The thickness of the attached gingiva was higher in males than females and showed significant difference in the maxillary arch for males and females, whereas it was non-significant in the mandibular arch [<xref ref-type="table" rid="T3">Table 3</xref>].</p><table-wrap id="T3" position="float"><label>Table 3</label><caption><p>Mean thickness of attached gingiva in different sex groups separately for maxillary and mandibular arch</p></caption><graphic xlink:href="JISP-18-478-g004"></graphic></table-wrap><p>The mean thickness of the attached gingiva was 1.04 (±0.52) mm and 1.12 (±0.69) mm for the maxillary arch and mandibular arch, respectively. This shows that it was higher in mandible compared to maxilla but not significant (<italic>P</italic> > 0.0001). <xref ref-type="table" rid="T4">Table 4</xref> shows that the thickness of the attached gingiva significantly decreased with an increase in the width for both the arches.</p><table-wrap id="T4" position="float"><label>Table 4</label><caption><p>Correlation between width of attached gingiva with thickness separately for maxillary and mandibular arch</p></caption><graphic xlink:href="JISP-18-478-g005"></graphic></table-wrap></sec><sec sec-type="discussion" id="sec1-4"><title>DISCUSSION</title><p>Various methods have been employed by research workers and clinicians to measure the thickness of gingiva, which is of immense importance considering their use in many therapeutic modalities for correction of mucogingival problems. The literature search reveals very limited clinical trials performed in this area and among these, majority have measured the thickness of masticatory mucosa on the palatal aspect, with a consideration that few of the therapeutic modalities employ palatal donor tissue for correction of the mucogingival problems. However, there are several other modalities, which rely on the adequacy of width of attached gingiva and thickness of gingiva on the buccal or facial aspects. Measurements of these parameters have been calculated by non-invasive methods such as application of ultrasonic devices and cone-beam computed tomography. Although the former method is more comfortable for the patient, the authors found difficulty in obtaining reliable results on a consistent basis.[<xref rid="ref5" ref-type="bibr">5</xref><xref rid="ref6" ref-type="bibr">6</xref>] The latter method using CBCT reveals a high quality image of hard and soft tissue and allows measurements of the dimensions and relationships of these structures. However, this method is unable to distinguish between normal and inflamed gingiva, which have similar images acquired by CBCT.[<xref rid="ref7" ref-type="bibr">7</xref>] Hence, the measurements are likely to be unreliable in some of the cases. Transgingival sounding by means of a periodontal probe has also been used for this purpose, however, this method seems to be inconvenient for the patient because it is invasive and has been performed under infiltration local anesthesia.[<xref rid="ref8" ref-type="bibr">8</xref><xref rid="ref9" ref-type="bibr">9</xref>] The present study hence was conducted to determine the width and thickness of facial gingiva in the anterior sextant by a periodontal probe under adequate local anesthesia spray.</p><p>Analysis of the width of the gingiva and thickness was performed not only in three age groups i.e., younger (16-24 years), middle (25-39 years) and older (above 40 years) age groups, but the gender variability was also taken into consideration by having equal number of males and females (20 each) in all these age groups. There is lack of studies wherein age wise, gender wise and dental arch location specific comparisons of these parameters are reported. The results indicated that there was an increase in width of attached gingiva in both maxillary and mandibular arches, with the increasing age groups, which was more in the maxillary compared to mandibular arches. Also the width of the attached gingiva was found to be more in males as compared to females.</p><p>In our study, the thickness of the gingiva was found to be more in younger age group and reduced with the increasing age. These findings are similar to those reported by Vandana <italic>et al</italic>.[<xref rid="ref10" ref-type="bibr">10</xref>] which can be because of change in oral epithelium caused by age related thinning of the epithelium and diminished keratinization. In our study, the thickness of the gingiva was recorded only on the midfacial aspect, as there could be existing variations in respect of midfacial and interdental recordings because the alveolar bone contours are different in these areas, which might influence the soft tissue thickness. So the recordings of our study seem to be more appropriate.</p><p>These findings are contrary to the observations of Waraaswapati <italic>et al</italic>.[<xref rid="ref9" ref-type="bibr">9</xref>] who reported that the thickness of palatal masticatory mucosa also increased with the increasing age group. However, it may not be proper to compare the findings of facial gingiva with palatal mucosa in toto.</p><p>The study population in the present study comprised of equal number of males and females in each age group, which enabled comparisons with regards to gender. The results indicated that the thickness of attached gingiva was more in males as compared to the females in both maxillary and mandibular arches. These findings showed statistically significant differences for maxillary arches, whereas they were non-significant in mandibular arches. These results were consistent with those of Muller <italic>et al</italic>. 2000[<xref rid="ref11" ref-type="bibr">11</xref>] and Song <italic>et al</italic>. 2008[<xref rid="ref12" ref-type="bibr">12</xref>] and contrary to those reported by Barriviera <italic>et al</italic>. 2009[<xref rid="ref7" ref-type="bibr">7</xref>] and Studer <italic>et al</italic>. 1997.[<xref rid="ref8" ref-type="bibr">8</xref>] These variations in results can be attributed to the methods of measurements. In case of infiltration anesthesia, the thickness can be influenced by the anesthetic solution deposited, whereas in case of CBCT, the inflammatory tissues can lead to increase in measurements. Local anesthesia sprays with periodontal probe seem to be one of the best methods of assessing thickness of attached gingiva as the chances of errors are least likely to creep in.</p><p>One of the limitations of this study is the influence of the bucco-lingual tooth position within the alveolar process. Although adequate care was taken that the teeth were in proper arch positions, minor variations if any may also have effect on the gingival biotypes.</p></sec><sec sec-type="conclusion" id="sec1-5"><title>CONCLUSION</title><p>It has been demonstrated that the thickness of the gingiva reduces with an increase in age, whereas the width of the attached gingiva increases with increasing age in both maxillary and mandibular dental arches. With regards to gender, the males had greater thickness of attached gingiva when compared to female counterparts. 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