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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Association of Periodontal Diseases and Liver Fibrosis in Patients With HCV and/or HBV infection</title>
<author><name sortKey="Nagao, Yumiko" sort="Nagao, Yumiko" uniqKey="Nagao Y" first="Yumiko" last="Nagao">Yumiko Nagao</name>
<affiliation><nlm:aff id="aff47907">Department of Digestive Disease Information and Research, Kurume University School of Medicine, Kurume, Fukuoka, Japan</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kawahigashi, Yuji" sort="Kawahigashi, Yuji" uniqKey="Kawahigashi Y" first="Yuji" last="Kawahigashi">Yuji Kawahigashi</name>
<affiliation><nlm:aff id="aff47907">Department of Digestive Disease Information and Research, Kurume University School of Medicine, Kurume, Fukuoka, Japan</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Sata, Michio" sort="Sata, Michio" uniqKey="Sata M" first="Michio" last="Sata">Michio Sata</name>
<affiliation><nlm:aff id="aff47909">Research Center for Innovative Cancer Therapy, Kurume University School of Medicine, Kurume, Fukuoka, Japan</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">25737729</idno>
<idno type="pmc">4329233</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329233</idno>
<idno type="RBID">PMC:4329233</idno>
<idno type="doi">10.5812/hepatmon.23264</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">002C73</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002C73</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Association of Periodontal Diseases and Liver Fibrosis in Patients With HCV and/or HBV infection</title>
<author><name sortKey="Nagao, Yumiko" sort="Nagao, Yumiko" uniqKey="Nagao Y" first="Yumiko" last="Nagao">Yumiko Nagao</name>
<affiliation><nlm:aff id="aff47907">Department of Digestive Disease Information and Research, Kurume University School of Medicine, Kurume, Fukuoka, Japan</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kawahigashi, Yuji" sort="Kawahigashi, Yuji" uniqKey="Kawahigashi Y" first="Yuji" last="Kawahigashi">Yuji Kawahigashi</name>
<affiliation><nlm:aff id="aff47907">Department of Digestive Disease Information and Research, Kurume University School of Medicine, Kurume, Fukuoka, Japan</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Sata, Michio" sort="Sata, Michio" uniqKey="Sata M" first="Michio" last="Sata">Michio Sata</name>
<affiliation><nlm:aff id="aff47909">Research Center for Innovative Cancer Therapy, Kurume University School of Medicine, Kurume, Fukuoka, Japan</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Hepatitis Monthly</title>
<idno type="ISSN">1735-143X</idno>
<idno type="eISSN">1735-3408</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background:</title>
<p>Periodontal disease and systemic health are closely associated. However, there is no data supporting the association between periodontal disease and patients with liver diseases associated with hepatitis C virus (HCV) and/or hepatitis B virus (HBV) infection.</p>
</sec>
<sec><title>Objectives:</title>
<p>The aim of this study was to evaluate the association between periodontitis and progression of liver diseases in patients with HCV and/or HBV infection.</p>
</sec>
<sec><title>Patients and Methods:</title>
<p>In this retrospective study, 351 patients with HCV- and/or HBV-related liver diseases underwent screening for periodontal disease using the Salivaster® salivary occult blood test from February 2010 to June 2014. Furthermore, we examined the prevalence of fimbrillin (<italic>fimA</italic>
) genotype of <italic>Porphyromonas gingivalis</italic>
(P. gingivalis) in 28 HCV-infected patients visited at our hospital between January 2013 and June 2014. <italic>P. gingivalis</italic>
with <italic>fimA</italic>
genotype with types I to V was further detected using a PCR method.</p>
</sec>
<sec><title>Results:</title>
<p>Of 351 patients, 76 patients (group 1) had a strong positive result for salivary occult blood test and 275 patients (group 2) had weak positive or negative test results. Significant factors between the groups were obesity, level of AST, ALT, LDH, ALP, Alb, D.Bil, T.cho, AFP, platelets (Plt), IRI, HOMA-IR, current interferon (IFN) treatment and the daily frequency of tooth brushing. Between-groups analysis indicated that total protein (T.pro) level and liver fibrosis were significant factors. According to multivariate analysis, five factors were associated with periodontal disease as Plt count below 80000, brushing teeth only once a day, current IFN treatment, aged 65 years or older and obesity. The adjusted odds ratios for these five factors were 5.80, 3.46, 2.87, 2.50 and 2.33, respectively, and each was statistically significant. Twenty-eight saliva specimens had positive results for <italic>P. gingivalis</italic>
with <italic>fimA</italic>
genotype types I to V. The prevalence of <italic>fimA</italic>
genotype II was higher in 14 patients with liver cirrhosis or a history of hepatocellular carcinoma treatment (group B, 50.00%) than 14 patients with only hepatitis C (group A, 21.43%).</p>
</sec>
<sec><title>Conclusions:</title>
<p>Periodontitis might be associated with progression of viral liver disease; hence, controlling oral disease is essential for the prevention and management of liver fibrosis.</p>
</sec>
</div>
</front>
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</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Hepat Mon</journal-id>
<journal-id journal-id-type="iso-abbrev">Hepat Mon</journal-id>
<journal-id journal-id-type="doi">10.5812/hepatmon</journal-id>
<journal-id journal-id-type="publisher-id">Kowsar</journal-id>
<journal-title-group><journal-title>Hepatitis Monthly</journal-title>
</journal-title-group>
<issn pub-type="ppub">1735-143X</issn>
<issn pub-type="epub">1735-3408</issn>
<publisher><publisher-name>Kowsar</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">25737729</article-id>
<article-id pub-id-type="pmc">4329233</article-id>
<article-id pub-id-type="doi">10.5812/hepatmon.23264</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Association of Periodontal Diseases and Liver Fibrosis in Patients With HCV and/or HBV infection</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Nagao</surname>
<given-names>Yumiko</given-names>
</name>
<xref ref-type="aff" rid="aff47907">1</xref>
<xref ref-type="corresp" rid="cor47908">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kawahigashi</surname>
<given-names>Yuji</given-names>
</name>
<xref ref-type="aff" rid="aff47907">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sata</surname>
<given-names>Michio</given-names>
</name>
<xref ref-type="aff" rid="aff47909">2</xref>
</contrib>
</contrib-group>
<aff id="aff47907"><label>1</label>
Department of Digestive Disease Information and Research, Kurume University School of Medicine, Kurume, Fukuoka, Japan</aff>
<aff id="aff47909"><label>2</label>
Research Center for Innovative Cancer Therapy, Kurume University School of Medicine, Kurume, Fukuoka, Japan</aff>
<author-notes><corresp id="cor47908"><label>*</label>
Corresponding Author: Yumiko Nagao, Department of Digestive Disease Information and Research, Kurume University School of Medicine, Kurume, Fukuoka, 830-0011, Japan. Tel: +81-942317902, Fax: +81-942317820, E-mail: <email>Nagao Y: nagao@med.kurume-u.ac.jp</email>
</corresp>
</author-notes>
<pub-date pub-type="epub"><day>20</day>
<month>12</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="collection"><month>12</month>
<year>2014</year>
</pub-date>
<volume>14</volume>
<issue>12</issue>
<elocation-id>e23264</elocation-id>
<history><date date-type="received"><day>03</day>
<month>9</month>
<year>2014</year>
</date>
<date date-type="rev-recd"><day>06</day>
<month>10</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>08</day>
<month>11</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2014, Kowsar Corp.</copyright-statement>
<copyright-year>2014</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc/4.0/"><license-p><pmc-comment>CREATIVE COMMONS</pmc-comment>
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/4.0/">http://creativecommons.org/licenses/by-nc/4.0/</ext-link>
) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.</license-p>
</license>
</permissions>
<abstract><sec><title>Background:</title>
<p>Periodontal disease and systemic health are closely associated. However, there is no data supporting the association between periodontal disease and patients with liver diseases associated with hepatitis C virus (HCV) and/or hepatitis B virus (HBV) infection.</p>
</sec>
<sec><title>Objectives:</title>
<p>The aim of this study was to evaluate the association between periodontitis and progression of liver diseases in patients with HCV and/or HBV infection.</p>
</sec>
<sec><title>Patients and Methods:</title>
<p>In this retrospective study, 351 patients with HCV- and/or HBV-related liver diseases underwent screening for periodontal disease using the Salivaster® salivary occult blood test from February 2010 to June 2014. Furthermore, we examined the prevalence of fimbrillin (<italic>fimA</italic>
) genotype of <italic>Porphyromonas gingivalis</italic>
(P. gingivalis) in 28 HCV-infected patients visited at our hospital between January 2013 and June 2014. <italic>P. gingivalis</italic>
with <italic>fimA</italic>
genotype with types I to V was further detected using a PCR method.</p>
</sec>
<sec><title>Results:</title>
<p>Of 351 patients, 76 patients (group 1) had a strong positive result for salivary occult blood test and 275 patients (group 2) had weak positive or negative test results. Significant factors between the groups were obesity, level of AST, ALT, LDH, ALP, Alb, D.Bil, T.cho, AFP, platelets (Plt), IRI, HOMA-IR, current interferon (IFN) treatment and the daily frequency of tooth brushing. Between-groups analysis indicated that total protein (T.pro) level and liver fibrosis were significant factors. According to multivariate analysis, five factors were associated with periodontal disease as Plt count below 80000, brushing teeth only once a day, current IFN treatment, aged 65 years or older and obesity. The adjusted odds ratios for these five factors were 5.80, 3.46, 2.87, 2.50 and 2.33, respectively, and each was statistically significant. Twenty-eight saliva specimens had positive results for <italic>P. gingivalis</italic>
with <italic>fimA</italic>
genotype types I to V. The prevalence of <italic>fimA</italic>
genotype II was higher in 14 patients with liver cirrhosis or a history of hepatocellular carcinoma treatment (group B, 50.00%) than 14 patients with only hepatitis C (group A, 21.43%).</p>
</sec>
<sec><title>Conclusions:</title>
<p>Periodontitis might be associated with progression of viral liver disease; hence, controlling oral disease is essential for the prevention and management of liver fibrosis.</p>
</sec>
</abstract>
<kwd-group kwd-group-type="author"><kwd>Periodontal Disease</kwd>
<kwd>Hepatitis C virus</kwd>
<kwd>Hepatitis B Virus</kwd>
<kwd>Interferon</kwd>
<kwd>Fibrosis</kwd>
<kwd><italic>Porphyromonas gingivalis</italic>
</kwd>
<kwd>Fimbrillin</kwd>
</kwd-group>
</article-meta>
</front>
<body><sec id="sec77257"><title>1. Background</title>
<p>The incidence of hepatocellular carcinoma (HCC) is increasing worldwide. In Japan, about 35000 deaths are reported due to HCC annually (<xref rid="A23264R1" ref-type="bibr">1</xref>
). In Japan, patients infected with hepatitis C virus (HCV) are older than those in other countries (<xref rid="A23264R2" ref-type="bibr">2</xref>
, <xref rid="A23264R3" ref-type="bibr">3</xref>
). Therefore, for patients with a high risk of developing HCC, it is necessary to implement a multifaceted treatment plan including nutritional interventions, exercise and measures to promote overall general wellness in addition to drug therapy. Therapy using interferon (IFN), an antiviral agent, reduces the rate of occurrence of HCC and improves long-term prognosis (<xref rid="A23264R4" ref-type="bibr">4</xref>
-<xref rid="A23264R6" ref-type="bibr">6</xref>
). Whereas, it is shown that periodontal disease even minimal chronic inflammation of the periodontal tissue, is associated with various systemic diseases such as cardiovascular disease and type II diabetes mellitus (DM), as well as low preterm birth-weight in children (<xref rid="A23264R7" ref-type="bibr">7</xref>
-<xref rid="A23264R9" ref-type="bibr">9</xref>
). Periodontitis is the most common chronic infection in adults and caused by bacteria encountered in subgingival plaque. The most important and prevalent anaerobic gram-negative bacteria in subgingival area are <italic>Actinobacillus actinomycetemcomitans</italic>
(<italic>A. actinomycetemcomitans</italic>
), <italic>Porphyromonas gingivalis</italic>
(<italic>P. gingivalis</italic>
), <italic>Prevotella intermedia</italic>
(<italic>P. intermedia</italic>
) and <italic>Tannerella forsythensis</italic>
(T. forsythensis). These bacteria play an important role in the onset and subsequent development of periodontitis, participating in the formation of periodontal pocket, connective tissue destruction, and alveolar bone resorption by means of an immunopathogenic mechanism (<xref rid="A23264R10" ref-type="bibr">10</xref>
). Dental plaque is a naturally occurring microbial layer or biofilm, which develops on the tooth surface. Biofilm formation is an important step in the etiology of periodontal diseases. Biofilms are particularly important in the oral cavity where the primary colonizers, which attach to oral surfaces, can enhance colonization of other bacteria which are themselves poor colonizers (<xref rid="A23264R11" ref-type="bibr">11</xref>
). Periodontal bacterial DNA of <italic>P. gingivalis</italic>
has been detected in atherosclerotic lesions of aortic tissues using various polymerase chain reaction (PCR) techniques (<xref rid="A23264R12" ref-type="bibr">12</xref>
). <italic>P. gingivalis</italic>
, a gram-negative oral anaerobe, is strongly associated with chronic adult periodontitis (<xref rid="A23264R13" ref-type="bibr">13</xref>
). The major structural subunit protein of fimbriae, <italic>FimA</italic>
(fimbrillin), mediates the invasion of gingival epithelial cells by <italic>P. gingivalis</italic>
(<xref rid="A23264R14" ref-type="bibr">14</xref>
). The <italic>FimA</italic>
-coding gene, <italic>fimA</italic>
, was classified into several genotypes (<xref rid="A23264R15" ref-type="bibr">15</xref>
) and their correlations with severity of periodontal diseases were investigated (<xref rid="A23264R15" ref-type="bibr">15</xref>
, <xref rid="A23264R16" ref-type="bibr">16</xref>
). There are six variants of <italic>P. gingivalis</italic>
<italic>fimA</italic>
gene (types I to V and Ib). It was demonstrated that bacterial clones with types II, IV or Ib <italic>fimA</italic>
are invasive, whereas those with types I, III or V fimbriae are noninvasive (<xref rid="A23264R15" ref-type="bibr">15</xref>
, <xref rid="A23264R16" ref-type="bibr">16</xref>
). It was recently reported that infection with high-virulence <italic>P. gingivalis</italic>
might be a risk factor of development and progression of non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) (<xref rid="A23264R17" ref-type="bibr">17</xref>
). However, there are no data to support the association between periodontal disease and patients with liver diseases associated with HCV and/or hepatitis B virus (HBV) infection.</p>
</sec>
<sec id="sec77258"><title>2. Objectives</title>
<p>In this study, we retrospectively examined the impact of oral environment on liver disease state, especially whether the presence of periodontal disease has an influence on liver disease progression, and we assessed the effect of IFN therapy in patients with HCV-and/or HBV-related liver diseases.</p>
</sec>
<sec id="sec77271"><title>3. Patients and Methods</title>
<sec id="sec77261"><title>3.1. Patients</title>
<sec id="sec77259"><title>3.1.1. Study 1</title>
<p>A total of 433 consecutive patients had checkups for oral mucosal diseases at the Oral Medicine clinic of the Digestive Diseases Center at Kurume University Hospital, Japan from February 1, 2010 to June 17, 2014. At the clinic, each patient was examined by physicians and an oral surgeon. Of 433 subjects, nine were edentulous and had been using a complete denture, and 34 subjects did not undergo a salivary occult blood test to screen the presence of periodontal disease. After exclusion of subjects with normal livers (20 patients) and subjects who had liver disease unrelated to HCV-and/or HBV infection, such as those with autoimmune liver disease (totally 19 patients; fatty liver: 10 cases; primary biliary cirrhosis: 4 cases; autoimmune hepatitis and HCC: 1 case; NASH: 1 case; non-B, non-C liver cirrhosis (LC): 1 case; non-B, non-C LC and HCC: 1 case; drug-induced liver injury: 1 case), 351 patients were enrolled in this study (<xref ref-type="fig" rid="fig14904">Figure 1</xref>
).</p>
<fig id="fig14904" orientation="portrait" position="float"><label>Figure 1.</label>
<caption><title>Schema for the Subjects</title>
</caption>
<graphic xlink:href="hepatmon-14-12-23264-i001"></graphic>
</fig>
</sec>
<sec id="sec77260"><title>3.1.2. Study 2</title>
<p>The prevalence of <italic>fimA</italic>
fimbriae genotype was assessed in 28 HCV-infected patients visited at our hospital between January 2013 and June 2014. HBs antigen-positive patients were excluded as subjects.</p>
</sec>
</sec>
<sec id="sec77262"><title>3.2. Examination of Oral Mucosal Disease</title>
<p>Subjects were asked regarding their daily frequency of tooth brushing. Mucosal examination was performed using a headband fiber (50-100-10, Daiichi Medical Co., Ltd.) with a brightness of 34000 luces for mucosal examination. Oral biopsy was performed on some patients.</p>
</sec>
<sec id="sec77263"><title>3.3. Salivary Occult Blood Test</title>
<p>Salivary occult blood test examines the presence of blood derived mainly from the gingival tissue and released into the saliva. We examined the presence of periodontal disease as an indicator of periodontitis using the Salivaster® reagent-based test (Showa Yakuhin Kako Co. LTD, Tokyo, Japan) (<xref rid="A23264R18" ref-type="bibr">18</xref>
, <xref rid="A23264R19" ref-type="bibr">19</xref>
). The Salivaster® is a colorimetric test based on a catalytic reaction of hemoglobin in saliva inducing the formation of different colors ranging from yellow to dark blue. The principle of color reaction is similar to the test for blood in urine, but was developed for the particular viscosity of saliva. The occult blood reaction is classified according to three levels: double positive (++), positive (+) and negative (−). This method is reported to have a sensitivity of 75.9% and specificity of 90.5% to detect gingival inflammation (<xref rid="A23264R20" ref-type="bibr">20</xref>
).</p>
</sec>
<sec id="sec77264"><title>3.4. Measurement of Body Weight</title>
<p>Obesity was defined as a body mass index (BMI) equal to or more than 25 kg/m<sup>2</sup>
.</p>
</sec>
<sec id="sec77265"><title>3.5. Serological Assays</title>
<p>All subjects were tested for red blood cell (RBC) count, white blood cell (WBC) count, platelet (Plt) count, hemoglobin (Hb), insulin resistance index (IRI), zinc (Zn) level, fasting blood glucose (FBS), and HbA1c, and for the following liver function tests: serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), prothrombin time (PT), total bilirubin (T.Bil), direct bilirubin (D.Bil), total cholesterol (T.cho), total protein (T.pro), albumin (Alb) and C-reactive protein (CRP). HOMA-IR formula is: HOMA-IR = FBS × fasting insulin/405.</p>
</sec>
<sec id="sec77266"><title>3.6. Evaluation of Liver Diseases</title>
<p>Anti-HCV was measured using a chemiluminescent enzyme immunoassay kit (Lumipulse II HCV, Fujirebio; Tokyo, Japan). HCV RNA was analyzed in serum by quantitative PCR assay (COBAS AMPLICOR HCV MONITOR v 2.0 Test, COBAS AmpliPrep/COBAS Taq-Man HCV Test; Roche Molecular Systems, New Jersey, the US) (<xref rid="A23264R21" ref-type="bibr">21</xref>
, <xref rid="A23264R22" ref-type="bibr">22</xref>
). HCV genotype was determined by polymerase chain reaction assay, using a mixture of primers for the subtype, as reported previously (<xref rid="A23264R23" ref-type="bibr">23</xref>
). Ultrasonographic examination was performed for all patients to investigate the shape of liver and its lesions. Computed tomography was also performed for some patients. We used other possible predictors of progression of liver cirrhosis (LC), including serum Alb, T.Bil, prothrombin time and Plt.</p>
</sec>
<sec id="sec77267"><title>3.7. Evaluation of Extrahepatic Diseases</title>
<p>Diagnosis of type II DM was based on the American Diabetic Association (ADA) criteria of 1997. Hypertension was defined as a systolic blood pressure (SBP) of 140 mmHg or higher or a diastolic blood pressure (DBP) of 90 mmHg or higher, according to the criteria of JNC-VI of the International Hypertension Society. FT3, FT4 and thyroid stimulating hormone (TSH) were measured for all patients and thyroid sonography examination was performed for some. Examination of upper or lower gastrointestinal tract was performed for patients for whom it was deemed clinically necessary.</p>
</sec>
<sec id="sec77268"><title>3.8. Detection of P. gingivalis fimA Genotypes in 28 Patients (Study 2)</title>
<p>Analysis of <italic>P. gingivalis</italic>
, <italic>Aggregatibacter actinomycetemcomitans</italic>
(<italic>A. actinomycetemcomitans</italic>
), <italic>P. intermedia</italic>
, <italic>T. forsythensis</italic>
, <italic>Treponema denticol</italic>
(<italic>T. denticol</italic>
) and <italic>Fusobacterium necrophorum</italic>
(<italic>F. necrophorum</italic>
) bacteria was conducted by a clinical laboratory test company using a PCR-Invader assay (BML Inc., Tokyo, Japan) (<xref rid="A23264R24" ref-type="bibr">24</xref>
). <italic>P. gingivalis</italic>
with <italic>fimA</italic>
genotype types I to V was detected in 28 saliva specimens.</p>
</sec>
<sec id="sec77269"><title>3.9. Ethical Considerations</title>
<p>The investigation was explained to all participants. The study was conducted in full accordance with the ethical principles of the World Medical Association Declaration of Helsinki.</p>
<p>Study 1, The Ethical Committee of Kurume University concluded that this retrospective study was not included in the ethics guidelines, because it was a medical practice involving diagnosis and treatment. Study 2, The study protocol was approved by the Ethics Committee of Kurume University (reference number: 12240) in accordance with the Declaration of Helsinki. A written informed consent was obtained from each patient.</p>
</sec>
<sec id="sec77270"><title>3.10. Statistical Analysis</title>
<p>All data were expressed as mean ± standard error. Differences between the two groups were analyzed using Mann-Whitney U test, Wilcoxon’s test and the Fisher’s exact test. Differences were significant at P < 0.05 (two-tailed). Adjusted odds ratios were calculated using logistic regression analysis. All statistical analyses were conducted using JMP Version 11.1.1 software (SAS Institute, Cary, NC, USA). The level of statistical significance was defined as P < 0.05.</p>
</sec>
</sec>
<sec id="sec77276"><title>4. Results</title>
<sec id="sec77272"><title>4.1. Characteristics of 351 Patients in Study 1</title>
<p>In total, 351 patients aged 17 to 87 years, with an average age of 60.62 ± 11.26 years (<xref ref-type="table" rid="tbl19652">Table 1</xref>
) were recruited. 147 were men and 204 women. The liver disease in study 1 included acute hepatitis C (n = 1, 0.28%), chronic hepatitis C (CH-C) (n = 156, 44.44%), CH-C and fatty liver (n = 6, 1.71%), CH-C and HCC (n = 15, 4.27%), CH-C and alcoholic liver disease (n = 3, 0.85%), CH-C and autoimmune hepatitis (n = 2, 0.57%), past history of HCV infection (n = 1, 0.28%), CH-C during IFN (n = 66, 18.80%), CH-C during IFN and fatty liver (n = 1, 0.28%), CH-C during IFN, alcoholic liver disease (n = 2, 0.57%), CH-C during IFN, post HCV-related HCC (n = 3, 0.85%), CH-C post IFN (sustained virological response, SVR)(n = 23, 6.55%), CH-C post IFN (SVR) and fatty liver (n = 2, 0.57%), CH-C post IFN (SVR) and NAFLD (n = 1, 0.28%), CH-C post IFN (SVR) and post HCV-related HCC (n = 2, 0.57%), CH-C post IFN (SVR) and asymptomatic HBV carrier (n = 1, 0.28%), liver cirrhosis type C (LC-C) (n = 10, 2.85%), LC-C and HCC (n = 21, 5.98%), LC-C and HCC with autoimmune hepatitis (n = 1, 0.28%), LC-C during IFN (n = 13, 3.70%), LC-C during IFN and post HCV-related HCC (n = 2, 0.57%), LC-C post IFN (SVR) (n = 4, 1.14%), CH-C and B and HCC (n = 2, 0.57%), asymptomatic HBV carrier (n = 1, 0.28%), chronic hepatitis B (n = 6, 1.71%), chronic hepatitis B and fatty liver (n = 1, 0.28%), chronic hepatitis B during IFN (n = 1, 0.28%), LC type B (n = 2, 0.57%), LC type B and CH-C during IFN (n = 1, 0.28%) and a past history of HBV infection (n = 1, 0.28%). Complications are shown in <xref ref-type="table" rid="tbl19652">Table 1</xref>
. The results of salivary occult blood testing were as follows: double positive/++, n = 76; positive/+, n = 181 and negative/−, n = 94. We compared characteristics of 76 patients (group 1) who had a strong positive result for salivary occult blood test and 275 patients (group 2) with a weak positive or negative result.</p>
<table-wrap id="tbl19652" orientation="portrait" position="float"><label>Table 1.</label>
<caption><title> Factors Associated With a Positive Result for Salivary Occult Blood Test According to Univariate Analysis <sup><xref ref-type="table-fn" rid="fn16988">a</xref>
</sup>
</title>
</caption>
<table frame="hsides" rules="groups"><thead><tr><th style="text-align: left;" rowspan="1" colspan="1"></th>
<th style="vertical-align: top;" rowspan="1" colspan="1">Patients' Data</th>
<th style="vertical-align: top;" rowspan="1" colspan="1">Strong positive (Group 1) (n = 76)</th>
<th style="vertical-align: top;" rowspan="1" colspan="1">Weak positive or negative (Group 2) (n = 275)</th>
<th style="vertical-align: top;" rowspan="1" colspan="1">P Value</th>
</tr>
</thead>
<tbody><tr><td rowspan="1" colspan="1"><bold>Gender, No.</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">Male</td>
<td style="text-align: center;" rowspan="1" colspan="1">147</td>
<td style="text-align: center;" rowspan="1" colspan="1">39</td>
<td style="text-align: center;" rowspan="1" colspan="1">108</td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">Female</td>
<td style="text-align: center;" rowspan="1" colspan="1">204</td>
<td style="text-align: center;" rowspan="1" colspan="1">37</td>
<td style="text-align: center;" rowspan="1" colspan="1">167</td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>Age, y </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">60.62 ± 11.26</td>
<td style="text-align: center;" rowspan="1" colspan="1">62.36 ± 10.07</td>
<td style="text-align: center;" rowspan="1" colspan="1">60.14 ± 11.53</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>BMI, kg/m<sup>2</sup>
</bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">22.78 ± 3.29</td>
<td style="text-align: center;" rowspan="1" colspan="1">23.95 ± 3.72</td>
<td style="text-align: center;" rowspan="1" colspan="1">22.46 ±3.09</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0003</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>AST, U/I </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">48.93 ± 33.80</td>
<td style="text-align: center;" rowspan="1" colspan="1">60.41 ± 37.35</td>
<td style="text-align: center;" rowspan="1" colspan="1">45.76 ± 32.11</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0003</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>ALT, U/I </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">50.99 ± 41.92</td>
<td style="text-align: center;" rowspan="1" colspan="1">61.24 ± 43.53</td>
<td style="text-align: center;" rowspan="1" colspan="1">48.16 ± 41.10</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0021</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>LDH, U/I </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">198.89 ± 43.37</td>
<td style="text-align: center;" rowspan="1" colspan="1">208.63 ± 49.22</td>
<td style="text-align: center;" rowspan="1" colspan="1">196.17 ± 41.28</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0171</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>ALP, U/l </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">295.76 ± 139.29</td>
<td style="text-align: center;" rowspan="1" colspan="1">354.23 ±190.97</td>
<td style="text-align: center;" rowspan="1" colspan="1">279.48 ±116.38</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0005</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>T.pro, g/dL </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">7.60 ± 0.56</td>
<td style="text-align: center;" rowspan="1" colspan="1">7.69 ± 0.52</td>
<td style="text-align: center;" rowspan="1" colspan="1">7.58 ± 0.57</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0549</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>Alb, g/dL </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">4.15 ± 0.45</td>
<td style="text-align: center;" rowspan="1" colspan="1">4.02 ± 0.44</td>
<td style="text-align: center;" rowspan="1" colspan="1">4.18 ± 0.44</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0018</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>D.Bil, mg/dL </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.12 ± 0.12</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.18 ± 0.20</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.10 ± 0.08</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0003</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>FBS, mg/dL </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">105.50 ± 21.94</td>
<td style="text-align: center;" rowspan="1" colspan="1">108.69 ± 26.84</td>
<td style="text-align: center;" rowspan="1" colspan="1">104.63 ± 20.37</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>T.cho, mg/dL </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">173.18 ± 35.52</td>
<td style="text-align: center;" rowspan="1" colspan="1">164.77 ± 32.85</td>
<td style="text-align: center;" rowspan="1" colspan="1">175.45 ± 35.93</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0316</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>HbA1c, % </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">5.43 ± 0.69</td>
<td style="text-align: center;" rowspan="1" colspan="1">5.45 ± 0.79</td>
<td style="text-align: center;" rowspan="1" colspan="1">5.42 ± 0.67</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>AFP, ng/dL </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">23.13 ± 176.28</td>
<td style="text-align: center;" rowspan="1" colspan="1">63.42 ± 371.08</td>
<td style="text-align: center;" rowspan="1" colspan="1">11.79 ± 29.98</td>
<td style="text-align: center;" rowspan="1" colspan="1">< 0.0001</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>Plt, ×104/μL </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">14.91 ± 5.11</td>
<td style="text-align: center;" rowspan="1" colspan="1">12.91 ± 4.64</td>
<td style="text-align: center;" rowspan="1" colspan="1">15.46 ± 5.11</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0003</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>WBC, μL </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">4642.61 ± 1453.08</td>
<td style="text-align: center;" rowspan="1" colspan="1">4568.00 ± 1479.51</td>
<td style="text-align: center;" rowspan="1" colspan="1">4663.03 ±1447.83</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>Hb, g/dL </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">13.70 ± 1.63</td>
<td style="text-align: center;" rowspan="1" colspan="1">13.65 ± 1.66</td>
<td style="text-align: center;" rowspan="1" colspan="1">13.72 ± 1.63</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>IRI, μU/mL </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">14.15 ± 15.98</td>
<td style="text-align: center;" rowspan="1" colspan="1">17.13 ± 20.23</td>
<td style="text-align: center;" rowspan="1" colspan="1">13.31 ± 14.48</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0071</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>HOMA-IR </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">3.77 ± 4.89</td>
<td style="text-align: center;" rowspan="1" colspan="1">4.87 ± 7.03</td>
<td style="text-align: center;" rowspan="1" colspan="1">3.45 ± 4.02</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0036</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>CRP, mg/dL </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.09 ± 0.16</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.08 ± 010</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.09 ± 0.18</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>Zn, μg/dL </bold>
<sup><bold><xref ref-type="table-fn" rid="fn16989">b</xref>
</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">72.14 ± 14.83</td>
<td style="text-align: center;" rowspan="1" colspan="1">71.34 ± 15.92</td>
<td style="text-align: center;" rowspan="1" colspan="1">72.36 ± 14.54</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>Anti-HCV, positive %</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">340 (96.87)</td>
<td style="text-align: center;" rowspan="1" colspan="1">75 (98.68)</td>
<td style="text-align: center;" rowspan="1" colspan="1">265 (96.36)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>HBsAg, positive %</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">9 (2.56)</td>
<td style="text-align: center;" rowspan="1" colspan="1">0 (0)</td>
<td style="text-align: center;" rowspan="1" colspan="1">9 (3.27)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>Current IFN treatment, positive %</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">89 (25.36)</td>
<td style="text-align: center;" rowspan="1" colspan="1">30 (39.47)</td>
<td style="text-align: center;" rowspan="1" colspan="1">59(21.45)</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0014</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>Liver diseases</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">HCC or post HCC</td>
<td style="text-align: center;" rowspan="1" colspan="1">49 (13.96)</td>
<td style="text-align: center;" rowspan="1" colspan="1">14 (18.42)</td>
<td style="text-align: center;" rowspan="1" colspan="1">35(12.73)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">LC</td>
<td style="text-align: center;" rowspan="1" colspan="1">54 (15.38)</td>
<td style="text-align: center;" rowspan="1" colspan="1">17 (22.37)</td>
<td style="text-align: center;" rowspan="1" colspan="1">37(13.45)</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0566</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>Extrahepatic manifestations</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">Lichen planus, positive</td>
<td style="text-align: center;" rowspan="1" colspan="1">51 (14.53)</td>
<td style="text-align: center;" rowspan="1" colspan="1">11 (14.47)</td>
<td style="text-align: center;" rowspan="1" colspan="1">40 (14.55)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">Extrahepatic malignant tumor, positive</td>
<td style="text-align: center;" rowspan="1" colspan="1">27 (7.69)</td>
<td style="text-align: center;" rowspan="1" colspan="1">4 (5.26)</td>
<td style="text-align: center;" rowspan="1" colspan="1">23 (8.36)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">Diabetes mellitus, positive</td>
<td style="text-align: center;" rowspan="1" colspan="1">59 (16.81)</td>
<td style="text-align: center;" rowspan="1" colspan="1">17 (22.37)</td>
<td style="text-align: center;" rowspan="1" colspan="1">42 (15.27)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">Hypertension, positive</td>
<td style="text-align: center;" rowspan="1" colspan="1">103 (29.34)</td>
<td style="text-align: center;" rowspan="1" colspan="1">25 (32.89)</td>
<td style="text-align: center;" rowspan="1" colspan="1">78 (28.36)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">Heart disease, positive</td>
<td style="text-align: center;" rowspan="1" colspan="1">14 (3.99)</td>
<td style="text-align: center;" rowspan="1" colspan="1">4 (5.26)</td>
<td style="text-align: center;" rowspan="1" colspan="1">10 (3.64)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">Sjogren's syndrome, positive</td>
<td style="text-align: center;" rowspan="1" colspan="1">4 (1.14)</td>
<td style="text-align: center;" rowspan="1" colspan="1">1 (1.32)</td>
<td style="text-align: center;" rowspan="1" colspan="1">3 (1.09%)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">Thyroid disease, positive</td>
<td style="text-align: center;" rowspan="1" colspan="1">18 (5.13)</td>
<td style="text-align: center;" rowspan="1" colspan="1">6 (7.89)</td>
<td style="text-align: center;" rowspan="1" colspan="1">12 (4.36)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">Osteoporosis, positive</td>
<td style="text-align: center;" rowspan="1" colspan="1">13 (3.70)</td>
<td style="text-align: center;" rowspan="1" colspan="1">1 (1.32)</td>
<td style="text-align: center;" rowspan="1" colspan="1">12 (4.36)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">Others, positive</td>
<td style="text-align: center;" rowspan="1" colspan="1">79 (22.51)</td>
<td style="text-align: center;" rowspan="1" colspan="1">17 (22.37)</td>
<td style="text-align: center;" rowspan="1" colspan="1">62 (22.55)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>Brushing teeth</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">Twice or more a day</td>
<td style="text-align: center;" rowspan="1" colspan="1">281 (80.06)</td>
<td style="text-align: center;" rowspan="1" colspan="1">50 (65.79)</td>
<td style="text-align: center;" rowspan="1" colspan="1">231 (84.00)</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0004</td>
</tr>
<tr><td style="padding-left: 20pt;" rowspan="1" colspan="1">Only once a day</td>
<td style="text-align: center;" rowspan="1" colspan="1">61 (17.38)</td>
<td style="text-align: center;" rowspan="1" colspan="1">25 (32.89)</td>
<td style="text-align: center;" rowspan="1" colspan="1">36 (13.09)</td>
<td style="text-align: center;" rowspan="1" colspan="1">< 0.0001</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>Smoking history, yes</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">168 (47.86)</td>
<td style="text-align: center;" rowspan="1" colspan="1">43 (56.58)</td>
<td style="text-align: center;" rowspan="1" colspan="1">125 (45.45)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>History of alcohol intake, yes</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">174 (49.57)</td>
<td style="text-align: center;" rowspan="1" colspan="1">39 (51.32)</td>
<td style="text-align: center;" rowspan="1" colspan="1">135 (49.09)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr><td rowspan="1" colspan="1"><bold>Subjective symptom of taste disorder, positive</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">16 (4.56)</td>
<td style="text-align: center;" rowspan="1" colspan="1">1 (1.32)</td>
<td style="text-align: center;" rowspan="1" colspan="1">15 (5.45)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
</tbody>
</table>
<table-wrap-foot><fn id="fn16988"><p><sup>a</sup>
Abbreviations: HCV, hepatitis C virus; LC-C, liver cirrhosis type C; HCC, hepatocellular carcinoma; IFN, interferon; NS, not significant; AST, aspartate aminotransferase; ALT, alanine aminotransferase; LDH, lactate dehydrogenase; ALP, alkaline phosphatase; TP, total protein; Alb, albumin; D.Bil, direct bilirubin; T.cho, total cholesterol; Plt, platelets.</p>
</fn>
<fn id="fn16989"><p><sup>b</sup>
Data are presented as No. (%) or Mean ± SD.</p>
</fn>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="sec77273"><title>4.2. Factors Associated with a Positive Result for Salivary Occult Blood Test According to Univariate Analysis (study 1)</title>
<p>Univariate analyses demonstrated significant associations between group 1 and group 2 (<xref ref-type="table" rid="tbl19652">Table 1</xref>
). Significant factors for groups were obesity (P = 0.0003), AST (P = 0.0003), ALT (P = 0.0021), LDH (P = 0.0171), ALP (P = 0.0005), Alb (P = 0.0018), D.Bil (P = 0.0003), T.cho (P = 0.0316), AFP (P < 0.0001), Plt (P = 0.0003), IRI (P = 0.0071), HOMA-IR (P = 0.0036), current IFN treatment (P = 0.0014) and daily frequency of tooth brushing (P < 0.0001). Between the two groups, T.pro level and liver fibrosis were significant factors. There were no significant differences in the levels of FBS, HbA1c, WBC, Hb, CRP, Zn, presence of HCC and extrahepatic manifestations, history of smoking and alcohol intake and taste disorder between the two groups.</p>
</sec>
<sec id="sec77274"><title>4.3. Multivariate Analysis (study 1)</title>
<p>All variables in the univariate analyses were included in the multivariate analysis (<xref ref-type="table" rid="tbl19653">Table 2</xref>
). According to multivariate analysis, five factors were associated with a strong positive result for salivary occult blood test, suggestive of the presence of periodontal disease: i.e. Plt count below 80000, brushing teeth only once a day, current IFN treatment, aged 65 years or older and obesity (BMI ≥ 25 kg/m<sup>2</sup>
). The adjusted odds ratios for these five factors were 5.80, 3.46, 2.87, 2.50 and 2.33, respectively, and each was statistically significant.</p>
<table-wrap id="tbl19653" orientation="portrait" position="float"><label>Table 2.</label>
<caption><title> Factors Associated With a Strong Positive Result for Salivary Occult Blood Test According to Multivariate Analysis <sup><xref ref-type="table-fn" rid="fn16991">a</xref>
</sup>
</title>
</caption>
<table frame="hsides" rules="groups"><thead><tr><th style="text-align: left;" rowspan="1" colspan="1"></th>
<th rowspan="1" colspan="1">Odds Ratio</th>
<th rowspan="1" colspan="1">95% CI</th>
<th rowspan="1" colspan="1">P Value</th>
</tr>
</thead>
<tbody><tr style="vertical-align:top;"><td rowspan="1" colspan="1"><bold>PLT < 8.0, × 10</bold>
<sup><bold>4</bold>
</sup>
<bold>/μL</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">5.80</td>
<td style="text-align: center;" rowspan="1" colspan="1">[2.30, 14.92]</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0002</td>
</tr>
<tr style="vertical-align:top;"><td rowspan="1" colspan="1"><bold>Brushing teeth only once a day</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">3.46</td>
<td style="text-align: center;" rowspan="1" colspan="1">[1.78, 6.76]</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0003</td>
</tr>
<tr style="vertical-align:top;"><td rowspan="1" colspan="1"><bold>Current IFN treatment</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">2.87</td>
<td style="text-align: center;" rowspan="1" colspan="1">[1.52, 5.43]</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0012</td>
</tr>
<tr style="vertical-align:top;"><td rowspan="1" colspan="1"><bold>65 years or older</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">2.50</td>
<td style="text-align: center;" rowspan="1" colspan="1">[1.37, 4.63]</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0028</td>
</tr>
<tr style="vertical-align:top;"><td rowspan="1" colspan="1"><bold>Obesity, BMI ≥ 25 kg/m</bold>
<sup><bold>2</bold>
</sup>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">2.33</td>
<td style="text-align: center;" rowspan="1" colspan="1">[1.22, 4.39]</td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0103</td>
</tr>
</tbody>
</table>
<table-wrap-foot><fn id="fn16991"><p><sup>a</sup>
Abbreviation: PLT, platelets.</p>
</fn>
</table-wrap-foot>
</table-wrap>
</sec>
<sec id="sec77275"><title>4.4. Prevalence of fimA Genotype Among HCV-Infected Patients</title>
<p>Among 28 patients, 14 had only hepatitis C (group A), and 14 had LC or a history of HCC treatment (group B) (<xref ref-type="table" rid="tbl19654">Table 3</xref>
). The prevalence of <italic>fimA</italic>
genotype II was higher in group B (50.00%) than group A (21.43%).</p>
<table-wrap id="tbl19654" orientation="portrait" position="float"><label>Table 3.</label>
<caption><title> Prevalence of <italic>fim A</italic>
Genotype Among HCV-Infected Patients (n = 14)<sup><xref ref-type="table-fn" rid="fn16990">a</xref>
</sup>
</title>
</caption>
<table frame="hsides" rules="groups"><thead><tr><th style="text-align: left;" rowspan="1" colspan="1"></th>
<th style="vertical-align: top;" rowspan="1" colspan="1">Only CH-C Group A</th>
<th style="vertical-align: top;" rowspan="1" colspan="1">LC or HCC Group B</th>
<th style="vertical-align: top;" rowspan="1" colspan="1">P Value</th>
</tr>
</thead>
<tbody><tr style="vertical-align:top;"><td rowspan="1" colspan="1"><bold>Gender</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1">0.0219</td>
</tr>
<tr style="vertical-align:top;"><td style="padding-left: 20pt;" rowspan="1" colspan="1">Male</td>
<td style="text-align: center;" rowspan="1" colspan="1">5</td>
<td style="text-align: center;" rowspan="1" colspan="1">11</td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
</tr>
<tr style="vertical-align:top;"><td style="padding-left: 20pt;" rowspan="1" colspan="1">Female</td>
<td style="text-align: center;" rowspan="1" colspan="1">9</td>
<td style="text-align: center;" rowspan="1" colspan="1">3</td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
</tr>
<tr style="vertical-align:top;"><td rowspan="1" colspan="1"><bold>Age, y</bold>
</td>
<td style="text-align: center;" rowspan="1" colspan="1">70.79 ± 5.28</td>
<td style="text-align: center;" rowspan="1" colspan="1">69.15 ± 10.80</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr style="vertical-align:top;"><td rowspan="1" colspan="1"><italic><bold>P.gingivalisfimA</bold>
</italic>
</td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
<td style="text-align: center;" rowspan="1" colspan="1"></td>
</tr>
<tr style="vertical-align:top;"><td style="padding-left: 20pt;" rowspan="1" colspan="1">Genotype I</td>
<td style="text-align: center;" rowspan="1" colspan="1">3 (21.43)</td>
<td style="text-align: center;" rowspan="1" colspan="1">3 (21.43)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr style="vertical-align:top;"><td style="padding-left: 20pt;" rowspan="1" colspan="1">Genotype I and II</td>
<td style="text-align: center;" rowspan="1" colspan="1">0 (0.00)</td>
<td style="text-align: center;" rowspan="1" colspan="1">1 (7.14)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr style="vertical-align:top;"><td style="padding-left: 20pt;" rowspan="1" colspan="1">Genotype II</td>
<td style="text-align: center;" rowspan="1" colspan="1">3 (21.43)</td>
<td style="text-align: center;" rowspan="1" colspan="1">7 (50.00)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr style="vertical-align:top;"><td style="padding-left: 20pt;" rowspan="1" colspan="1">Genotype III</td>
<td style="text-align: center;" rowspan="1" colspan="1">1 (7.14)</td>
<td style="text-align: center;" rowspan="1" colspan="1">0 (0.00)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr style="vertical-align:top;"><td style="padding-left: 20pt;" rowspan="1" colspan="1">Genotype IV</td>
<td style="text-align: center;" rowspan="1" colspan="1">2 (14.29)</td>
<td style="text-align: center;" rowspan="1" colspan="1">0 (0.00)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr style="vertical-align:top;"><td style="padding-left: 20pt;" rowspan="1" colspan="1">Genotype V</td>
<td style="text-align: center;" rowspan="1" colspan="1">0 (0.00)</td>
<td style="text-align: center;" rowspan="1" colspan="1">1 (7.14)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
<tr style="vertical-align:top;"><td style="padding-left: 20pt;" rowspan="1" colspan="1">Below detection sensitivity limits</td>
<td style="text-align: center;" rowspan="1" colspan="1">5 (35.71)</td>
<td style="text-align: center;" rowspan="1" colspan="1">2 (14.29)</td>
<td style="text-align: center;" rowspan="1" colspan="1">NS</td>
</tr>
</tbody>
</table>
<table-wrap-foot><fn id="fn16990"><p><sup>a</sup>
Data are presented as No. (%) or Mean ± SD.</p>
</fn>
</table-wrap-foot>
</table-wrap>
</sec>
</sec>
<sec id="sec77277"><title>5. Discussion</title>
<p>HCC is one of the most common forms of malignancy, particularly in Japan. The most important risk factors of HCC are chronic hepatitis C and B and cirrhosis. Japanese patients with chronic hepatitis C are generally treated with IFN, but an additional strategy for preventing HCC development is urgent need for LC patients. In our retrospective analysis, we found an association between periodontitis and IFN therapy. We previously reported that dental problems delayed the initiation of IFN therapy for HCV-infected patients (<xref rid="A23264R25" ref-type="bibr">25</xref>
). HCV-infected patients treated with IFN therapy should be managed by intensive oral care because of lowered resistance to infection during the therapy. Regarding a potential link between periodontal diseases and liver fibrosis in patients with HCV- and/or HBV-related liver diseases, patients with a strong positive result for salivary occult blood test, indicative of periodontal disease, had a higher prevalence of indices associated with liver disease progression (e.g. elevated AFP level, thrombocytopenia, hypoalbuminemia and hyperbilirubinemia) compared to patients with slight or no periodontitis. Moreover, the prevalence of <italic>fimA</italic>
genotype II was higher in patients with LC (50.00%) than those with only hepatitis C (21.43%). In summary, our results showed a possible association between periodontitis and progression of viral liver disease. We found obesity as a risk factor of periodontal disease among patients with viral liver disease. The meta-analysis showed a significant association between obesity and periodontitis (OR = 1.30 [95% Confidence Interval (CI), 1.25 - 1.35]) and between mean BMI and periodontal disease (mean difference = 2.75) (<xref rid="A23264R26" ref-type="bibr">26</xref>
). Obesity, a key component of metabolic syndrome, has been linked to a higher frequency of cancers in a variety of tissues including the liver (<xref rid="A23264R27" ref-type="bibr">27</xref>
). Potential mediators of obesity-related HCC include lipotoxicity, changes in the gut microbiome, an imbalance in proinflammatory/antiinflammatory cytokines, stimulation of insulin-like growth factor 1 (IGF-1) axis by hyperinsulinemia, reduced adiponectin secretion and increased leptin levels. Patients with periodontitis had a significantly lower frequency of tooth brushing. Joint support involving a dentist, a primary care physician and particularly a hepatologist is necessary for patients with liver disease to appreciate the importance of good oral hygiene. Currently, in Japan, no such support system exists. Several studies showed an association between HCV infection and insulin resistance (<xref rid="A23264R28" ref-type="bibr">28</xref>
, <xref rid="A23264R29" ref-type="bibr">29</xref>
), and severe periodontal disease is known to cause insulin resistance (<xref rid="A23264R30" ref-type="bibr">30</xref>
). Problems faced by HCV-infected individuals regarding oral healthcare may be due to decreased salivary flow rate, elicitation of periodontal disease by insulin resistance and difficulties with radical dental treatment for patients with liver disease, because of complications such as prolonged bleeding. Henderson and colleagues reported that HCV-infected patients may sometimes face discrimination by practitioners (<xref rid="A23264R31" ref-type="bibr">31</xref>
). The role of oral fluid on periodontal condition of patients with viral hepatitis is unknown. However, HBV and HCV markers in gingival crevicular fluid (GCF) have been studied. GCF, a serum transudate or inflammatory exudate, can be collected from gingival crevice surrounding the teeth. GCF contains microbial plaque, host inflammatory cells, host tissue and serum-derivers factors (<xref rid="A23264R32" ref-type="bibr">32</xref>
). Hepatitis B surface antigen (HBsAg) was detected in GCF and whole saliva in approximately 90% of samples from seropositive patients, implying that GCF is likely the source of HBsAg in saliva (<xref rid="A23264R33" ref-type="bibr">33</xref>
). Anti-HCV antibodies and HCV RNA were also detected in GCF and saliva of HCV-positive patients (<xref rid="A23264R34" ref-type="bibr">34</xref>
-<xref rid="A23264R36" ref-type="bibr">36</xref>
). These data support the concept that GCF may be a significant source of hepatitis virus in saliva. AST is an enzyme found at increased concentrations in red blood cells, liver, heart and muscles. Persson et al. demonstrated that AST levels can be used to assess the presence and extent of periodontal inflammation (<xref rid="A23264R37" ref-type="bibr">37</xref>
). Median AST levels were significantly higher for the sites experiencing clinical attachment loss in comparison to sites not experiencing attachment loss. Further studies are needed to clarify the association between inflammatory substances in GCF and viral hepatitis. Liver fibrosis can increase the risk of periodontal diseases, which are more prevalent among elderly. The prevalence of periodontal disease among HCV-infected patients in Japan would be expected to increase. Similarly, in Japan the number of older patients with HCV is increasing. A cooperative system to help minimize the impact of periodontitis on the progression of liver disease is urgently needed. In conclusion, our retrospective analysis showed that periodontitis might be associated with progression of viral liver disease; therefore, controlling oral disease is essential for prevention and management of liver fibrosis.</p>
</sec>
</body>
<back><ack><p>This study was supported in part by a Grant-in-Aid for Scientific Research (C) (No.25463274) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.</p>
</ack>
<fn-group><fn id="afn34928" fn-type="other"><p><bold>Authors' Contributions:</bold>
Yumiko Nagao performed most of data collection, designed the study, performed the statistical analysis and drafted the manuscript. Yuji Kawahigashi provided comments during manuscript preparation. Michio Sata contributed to data collections and clinical diagnosis. All authors read and approved the final version of the manuscript.</p>
</fn>
<fn id="afn34929" fn-type="supported-by"><p><bold>Finding/Support:</bold>
This study was supported in part by a Grant-in-Aid for Scientific Research (C) (No.25463274) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.</p>
</fn>
</fn-group>
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