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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Comparison of the eFficacy of Nystatin and Fluconazole Incorporated into Tissue Conditioner on the In Vitro Attachment and Colonization of <italic>Candida Albicans</italic></title><author><name sortKey="Falah Tafti, Abbas" sort="Falah Tafti, Abbas" uniqKey="Falah Tafti A" first="Abbas" last="Falah-Tafti">Abbas Falah-Tafti</name><affiliation><nlm:aff id="aff1">Assistant Professor, Department of Prosthodontics, School of Dentistry, Yazd University of Medical Sciences, Yazd, Iran</nlm:aff></affiliation></author><author><name sortKey="Jafari, Abbas Ali" sort="Jafari, Abbas Ali" uniqKey="Jafari A" first="Abbas Ali" last="Jafari">Abbas Ali Jafari</name><affiliation><nlm:aff id="aff2">Associate Professor, Department of Parasitology, School of Medicine, Yazd University of Medical Sciences, Yazd, Iran</nlm:aff></affiliation></author><author><name sortKey="Lotfi Kamran, Mohammad Hossein" sort="Lotfi Kamran, Mohammad Hossein" uniqKey="Lotfi Kamran M" first="Mohammad Hossein" last="Lotfi-Kamran">Mohammad Hossein Lotfi-Kamran</name><affiliation><nlm:aff id="aff1">Assistant Professor, Department of Prosthodontics, School of Dentistry, Yazd University of Medical Sciences, Yazd, Iran</nlm:aff></affiliation></author><author><name sortKey="Fallahzadeh, Hossein" sort="Fallahzadeh, Hossein" uniqKey="Fallahzadeh H" first="Hossein" last="Fallahzadeh">Hossein Fallahzadeh</name><affiliation><nlm:aff id="aff3">Associate Professor, Department of Biostatistics and Epidemiology, School of Public Health, Yazd University of Medical Sciences, Yazd, Iran</nlm:aff></affiliation></author><author><name sortKey="Hayan, Reyhana Sadat" sort="Hayan, Reyhana Sadat" uniqKey="Hayan R" first="Reyhana Sadat" last="Hayan">Reyhana Sadat Hayan</name><affiliation><nlm:aff id="aff4">Dentist, Yazd University of Medical Sciences, Yazd, Iran</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21448442</idno><idno type="pmc">3065338</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065338</idno><idno type="RBID">PMC:3065338</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">002B97</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002B97</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Comparison of the eFficacy of Nystatin and Fluconazole Incorporated into Tissue Conditioner on the In Vitro Attachment and Colonization of <italic>Candida Albicans</italic></title><author><name sortKey="Falah Tafti, Abbas" sort="Falah Tafti, Abbas" uniqKey="Falah Tafti A" first="Abbas" last="Falah-Tafti">Abbas Falah-Tafti</name><affiliation><nlm:aff id="aff1">Assistant Professor, Department of Prosthodontics, School of Dentistry, Yazd University of Medical Sciences, Yazd, Iran</nlm:aff></affiliation></author><author><name sortKey="Jafari, Abbas Ali" sort="Jafari, Abbas Ali" uniqKey="Jafari A" first="Abbas Ali" last="Jafari">Abbas Ali Jafari</name><affiliation><nlm:aff id="aff2">Associate Professor, Department of Parasitology, School of Medicine, Yazd University of Medical Sciences, Yazd, Iran</nlm:aff></affiliation></author><author><name sortKey="Lotfi Kamran, Mohammad Hossein" sort="Lotfi Kamran, Mohammad Hossein" uniqKey="Lotfi Kamran M" first="Mohammad Hossein" last="Lotfi-Kamran">Mohammad Hossein Lotfi-Kamran</name><affiliation><nlm:aff id="aff1">Assistant Professor, Department of Prosthodontics, School of Dentistry, Yazd University of Medical Sciences, Yazd, Iran</nlm:aff></affiliation></author><author><name sortKey="Fallahzadeh, Hossein" sort="Fallahzadeh, Hossein" uniqKey="Fallahzadeh H" first="Hossein" last="Fallahzadeh">Hossein Fallahzadeh</name><affiliation><nlm:aff id="aff3">Associate Professor, Department of Biostatistics and Epidemiology, School of Public Health, Yazd University of Medical Sciences, Yazd, Iran</nlm:aff></affiliation></author><author><name sortKey="Hayan, Reyhana Sadat" sort="Hayan, Reyhana Sadat" uniqKey="Hayan R" first="Reyhana Sadat" last="Hayan">Reyhana Sadat Hayan</name><affiliation><nlm:aff id="aff4">Dentist, Yazd University of Medical Sciences, Yazd, Iran</nlm:aff></affiliation></author></analytic><series><title level="j">Dental Research Journal</title><idno type="ISSN">1735-3327</idno><idno type="eISSN">2008-0255</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="st1"><title>Background:</title><p>Denture stomatitis is a common oral lesion following the use of ill-fitting dentures. A layer of tissue conditioner is usually used to improve adaptation of the denture. These liners can support the <italic>in vivo</italic> adhesion and colonization of the oral <italic>Candida</italic>. The aim of this study was to evaluate the efficacy of the two common antifungal agents mixed with tissue conditioner against <italic>Candida albicans</italic>.</p></sec><sec id="st2"><title>Methods:</title><p>Tissue conditioner disks (Acrosoft) with 5mm diameter and 1mm thickness containing different concentrations of nystatin and fluconazole (1%, 3%, 5%, 10% wt/wt) as well as disks with no antifungal agents (8 disks for each group) were prepared for experimental biofilm formation by inoculation with <italic>Candida albicans</italic> cell suspensions. The specimens were incubated in cell culture microtiter plate wells containing Sabouraud's broth in a rotator shaker at 30°C for 48 hours. Then, the specimens were rinsed and sonicated in sterile water to remove surface organisms. The attached yeasts were enumerated by inoculation of the yeast suspension on Sabouraud's agar. The data was compared using Kruskal-Wallis and Dunn's tests using prism software. P value less than 0.05 was considered significant.</p></sec><sec id="st3"><title>Results:</title><p>The 1% to 10% mixture of nystatin and tissue conditioner completely inhibited the attachment and colonization of <italic>Candida albicans</italic>, although for fluconazole only a 10% concentration caused complete inhibition. Nystatin showed a potentially higher effect in inhibition of candida attachment and colonization (P = 0.0001) compared to that of fluconazole and a statistically significant difference was seen between 5% and 1% fluconazole (P = 0.0001).</p></sec><sec id="st4"><title>Conclusion:</title><p>Tissue conditioner with 1% to 10% nystatin or 10% fluconazole can completely inhibit the adhesion and colonization of <italic>Candida albicans</italic>.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Wilson, J" uniqKey="Wilson J">J Wilson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Budtz Jlrgensen, E" uniqKey="Budtz Jlrgensen E">E Budtz-Jlrgensen</name></author><author><name sortKey="Mojon, P" uniqKey="Mojon P">P Mojon</name></author><author><name sortKey="Banon Clement, Jm" uniqKey="Banon Clement J">JM Banon-Clement</name></author><author><name sortKey="Baehni, P" uniqKey="Baehni P">P Baehni</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Espinoza, I" uniqKey="Espinoza I">I Espinoza</name></author><author><name sortKey="Rojas, R" uniqKey="Rojas R">R Rojas</name></author><author><name sortKey="Aranda, W" uniqKey="Aranda W">W Aranda</name></author><author><name sortKey="Gamonal, J" uniqKey="Gamonal J">J Gamonal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Darwazeh, Am" uniqKey="Darwazeh A">AM Darwazeh</name></author><author><name sortKey="Al Refai, S" uniqKey="Al Refai S">S Al Refai</name></author><author><name sortKey="Al Mojaiwel, S" uniqKey="Al Mojaiwel S">S Al Mojaiwel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barbeau, J" uniqKey="Barbeau J">J Barbeau</name></author><author><name sortKey="Seguin, J" uniqKey="Seguin J">J Seguin</name></author><author><name sortKey="Goulet, Jp" uniqKey="Goulet J">JP Goulet</name></author><author><name sortKey="De Koninck, L" uniqKey="De Koninck L">L de Koninck</name></author><author><name sortKey="Avon, Sl" uniqKey="Avon S">SL Avon</name></author><author><name sortKey="Lalonde, B" uniqKey="Lalonde B">B Lalonde</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Monsenego, P" uniqKey="Monsenego P">P Monsenego</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bissell, V" uniqKey="Bissell V">V Bissell</name></author><author><name sortKey="Felix, Dh" uniqKey="Felix D">DH Felix</name></author><author><name sortKey="Wray, D" uniqKey="Wray D">D Wray</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moura, Js" uniqKey="Moura J">JS Moura</name></author><author><name sortKey="Da Silva, Wj" uniqKey="Da Silva W">WJ da Silva</name></author><author><name sortKey="Pereira, T" uniqKey="Pereira T">T Pereira</name></author><author><name sortKey="Bel Cury, Aa" uniqKey="Bel Cury A">AA Bel Cury</name></author><author><name sortKey="Rodrigues Garcia, Rc" uniqKey="Rodrigues Garcia R">RC Rodrigues Garcia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fanello, S" uniqKey="Fanello S">S Fanello</name></author><author><name sortKey="Bouchara, Jp" uniqKey="Bouchara J">JP Bouchara</name></author><author><name sortKey="Sauteron, M" uniqKey="Sauteron M">M Sauteron</name></author><author><name sortKey="Delbos, V" uniqKey="Delbos V">V Delbos</name></author><author><name sortKey="Parot, E" uniqKey="Parot E">E Parot</name></author><author><name sortKey="Marot Leblond, A" uniqKey="Marot Leblond A">A Marot-Leblond</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chander, S" uniqKey="Chander S">S Chander</name></author><author><name sortKey="Hill, M" uniqKey="Hill M">M Hill</name></author><author><name sortKey="Moore, D" uniqKey="Moore D">D Moore</name></author><author><name sortKey="Morrow, L" uniqKey="Morrow L">L Morrow</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Waters, Mg" uniqKey="Waters M">MG Waters</name></author><author><name sortKey="Williams, Dw" uniqKey="Williams D">DW Williams</name></author><author><name sortKey="Jagger, Rg" uniqKey="Jagger R">RG Jagger</name></author><author><name sortKey="Lewis, Ma" uniqKey="Lewis M">MA Lewis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kulak, Y" uniqKey="Kulak Y">Y Kulak</name></author><author><name sortKey="Kadir, T" uniqKey="Kadir T">T Kadir</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ramage, G" uniqKey="Ramage G">G Ramage</name></author><author><name sortKey="Vandewalle, K" uniqKey="Vandewalle K">K Vandewalle</name></author><author><name sortKey="Wickes, Bl" uniqKey="Wickes B">BL Wickes</name></author><author><name sortKey="Lopez Ribot, Jl" uniqKey="Lopez Ribot J">JL Lopez-Ribot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weir, E" uniqKey="Weir E">E Weir</name></author><author><name sortKey="Lawlor, A" uniqKey="Lawlor A">A Lawlor</name></author><author><name sortKey="Whelan, A" uniqKey="Whelan A">A Whelan</name></author><author><name sortKey="Regan, F" uniqKey="Regan F">F Regan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valappil, Sp" uniqKey="Valappil S">SP Valappil</name></author><author><name sortKey="Pickup, Dm" uniqKey="Pickup D">DM Pickup</name></author><author><name sortKey="Carroll, Dl" uniqKey="Carroll D">DL Carroll</name></author><author><name sortKey="Hope, Ck" uniqKey="Hope C">CK Hope</name></author><author><name sortKey="Pratten, J" uniqKey="Pratten J">J Pratten</name></author><author><name sortKey="Newport, Rj" uniqKey="Newport R">RJ Newport</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Quinn, Dm" uniqKey="Quinn D">DM Quinn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Akiba, N" uniqKey="Akiba N">N Akiba</name></author><author><name sortKey="Hayakawa, I" uniqKey="Hayakawa I">I Hayakawa</name></author><author><name sortKey="Keh, Es" uniqKey="Keh E">ES Keh</name></author><author><name sortKey="Watanabe, A" uniqKey="Watanabe A">A Watanabe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Catalan, A" uniqKey="Catalan A">A Catalan</name></author><author><name sortKey="Pacheco, Jg" uniqKey="Pacheco J">JG Pacheco</name></author><author><name sortKey="Martinez, A" uniqKey="Martinez A">A Martinez</name></author><author><name sortKey="Mondaca, Ma" uniqKey="Mondaca M">MA Mondaca</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Espinel Ingroff, A" uniqKey="Espinel Ingroff A">A Espinel-Ingroff</name></author><author><name sortKey="Fothergill, A" uniqKey="Fothergill A">A Fothergill</name></author><author><name sortKey="Ghannoum, M" uniqKey="Ghannoum M">M Ghannoum</name></author><author><name sortKey="Manavathu, E" uniqKey="Manavathu E">E Manavathu</name></author><author><name sortKey="Ostrosky Zeichner, L" uniqKey="Ostrosky Zeichner L">L Ostrosky-Zeichner</name></author><author><name sortKey="Pfaller, M" uniqKey="Pfaller M">M Pfaller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Geerts, Ga" uniqKey="Geerts G">GA Geerts</name></author><author><name sortKey="Stuhlinger, Me" uniqKey="Stuhlinger M">ME Stuhlinger</name></author><author><name sortKey="Basson, Nj" uniqKey="Basson N">NJ Basson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jittapiromsak, N" uniqKey="Jittapiromsak N">N Jittapiromsak</name></author><author><name sortKey="Benjavongkulchai, E" uniqKey="Benjavongkulchai E">E Benjavongkulchai</name></author><author><name sortKey="Purnaveja, S" uniqKey="Purnaveja S">S Purnaveja</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rathore, P" uniqKey="Rathore P">P Rathore</name></author><author><name sortKey="Hegde, A" uniqKey="Hegde A">A Hegde</name></author><author><name sortKey="Ginjupalli, K" uniqKey="Ginjupalli K">K Ginjupalli</name></author><author><name sortKey="Upadhya, N" uniqKey="Upadhya N">N Upadhya</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Truhlar, Mr" uniqKey="Truhlar M">MR Truhlar</name></author><author><name sortKey="Shay, K" uniqKey="Shay K">K Shay</name></author><author><name sortKey="Sohnle, P" uniqKey="Sohnle P">P Sohnle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thomas, Cj" uniqKey="Thomas C">CJ Thomas</name></author><author><name sortKey="Nutt, Gm" uniqKey="Nutt G">GM Nutt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chow, Ck" uniqKey="Chow C">CK Chow</name></author><author><name sortKey="Matear, Dw" uniqKey="Matear D">DW Matear</name></author><author><name sortKey="Lawrence, Hp" uniqKey="Lawrence H">HP Lawrence</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dogan, A" uniqKey="Dogan A">A Dogan</name></author><author><name sortKey="Bek, B" uniqKey="Bek B">B Bek</name></author><author><name sortKey="Cevik, Nn" uniqKey="Cevik N">NN Cevik</name></author><author><name sortKey="Usanmaz, A" uniqKey="Usanmaz A">A Usanmaz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Douglas, Wh" uniqKey="Douglas W">WH Douglas</name></author><author><name sortKey="Clarke, Da" uniqKey="Clarke D">DA Clarke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saatchi, S" uniqKey="Saatchi S">S Saatchi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martindale, W" uniqKey="Martindale W">W Martindale</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Dent Res J (Isfahan)</journal-id><journal-id journal-id-type="publisher-id">DRJ</journal-id><journal-title-group><journal-title>Dental Research Journal</journal-title></journal-title-group><issn pub-type="ppub">1735-3327</issn><issn pub-type="epub">2008-0255</issn><publisher><publisher-name>Medknow Publications & Media Pvt Ltd</publisher-name><publisher-loc>India</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">21448442</article-id><article-id pub-id-type="pmc">3065338</article-id><article-id pub-id-type="publisher-id">DRJ-7-18</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>A Comparison of the eFficacy of Nystatin and Fluconazole Incorporated into Tissue Conditioner on the In Vitro Attachment and Colonization of <italic>Candida Albicans</italic></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Falah-Tafti</surname><given-names>Abbas</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Jafari</surname><given-names>Abbas Ali</given-names></name><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="corresp" rid="cor1"></xref></contrib><contrib contrib-type="author"><name><surname>Lotfi-Kamran</surname><given-names>Mohammad Hossein</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Fallahzadeh</surname><given-names>Hossein</given-names></name><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Hayan</surname><given-names>Reyhana Sadat</given-names></name><xref ref-type="aff" rid="aff4">4</xref></contrib></contrib-group><aff id="aff1"><label>1</label>Assistant Professor, Department of Prosthodontics, School of Dentistry, Yazd University of Medical Sciences, Yazd, Iran</aff><aff id="aff2"><label>2</label>Associate Professor, Department of Parasitology, School of Medicine, Yazd University of Medical Sciences, Yazd, Iran</aff><aff id="aff3"><label>3</label>Associate Professor, Department of Biostatistics and Epidemiology, School of Public Health, Yazd University of Medical Sciences, Yazd, Iran</aff><aff id="aff4"><label>4</label>Dentist, Yazd University of Medical Sciences, Yazd, Iran</aff><author-notes><corresp id="cor1">Correspondence to: Abbas Ali Jafari, <email xlink:href="jafariabbas@ssu.ac.ir">jafariabbas@ssu.ac.ir</email></corresp></author-notes><pub-date pub-type="ppub"><season>Winter</season><year>2010</year></pub-date><volume>7</volume><issue>1</issue><fpage>18</fpage><lpage>22</lpage><history><date date-type="received"><month>12</month><year>2009</year></date><date date-type="accepted"><month>5</month><year>2010</year></date></history><permissions><copyright-statement>Copyright: © Dental Research Journal</copyright-statement><copyright-year>2010</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><sec id="st1"><title>Background:</title><p>Denture stomatitis is a common oral lesion following the use of ill-fitting dentures. A layer of tissue conditioner is usually used to improve adaptation of the denture. These liners can support the <italic>in vivo</italic> adhesion and colonization of the oral <italic>Candida</italic>. The aim of this study was to evaluate the efficacy of the two common antifungal agents mixed with tissue conditioner against <italic>Candida albicans</italic>.</p></sec><sec id="st2"><title>Methods:</title><p>Tissue conditioner disks (Acrosoft) with 5mm diameter and 1mm thickness containing different concentrations of nystatin and fluconazole (1%, 3%, 5%, 10% wt/wt) as well as disks with no antifungal agents (8 disks for each group) were prepared for experimental biofilm formation by inoculation with <italic>Candida albicans</italic> cell suspensions. The specimens were incubated in cell culture microtiter plate wells containing Sabouraud's broth in a rotator shaker at 30°C for 48 hours. Then, the specimens were rinsed and sonicated in sterile water to remove surface organisms. The attached yeasts were enumerated by inoculation of the yeast suspension on Sabouraud's agar. The data was compared using Kruskal-Wallis and Dunn's tests using prism software. P value less than 0.05 was considered significant.</p></sec><sec id="st3"><title>Results:</title><p>The 1% to 10% mixture of nystatin and tissue conditioner completely inhibited the attachment and colonization of <italic>Candida albicans</italic>, although for fluconazole only a 10% concentration caused complete inhibition. Nystatin showed a potentially higher effect in inhibition of candida attachment and colonization (P = 0.0001) compared to that of fluconazole and a statistically significant difference was seen between 5% and 1% fluconazole (P = 0.0001).</p></sec><sec id="st4"><title>Conclusion:</title><p>Tissue conditioner with 1% to 10% nystatin or 10% fluconazole can completely inhibit the adhesion and colonization of <italic>Candida albicans</italic>.</p></sec></abstract><kwd-group><kwd>Candida</kwd><kwd>Fluconazole</kwd><kwd>Nystatin</kwd><kwd>Tissue conditioner</kwd></kwd-group></article-meta></front><body><sec sec-type="intro" id="sec1-1"><title>Introduction</title><p>Denture stomatitis (DS) is a chronic inflammatory condition of the palatal and alveolar mucosa underlying removable dental prostheses. Denture stomatitis is more commonly seen in the maxillary mucosa. The prevalence of denture stomatitis is varied from 15 to 65%<xref ref-type="bibr" rid="ref1">1</xref> and even more significant in the institutionalized denture wearing population at up to 72%.<xref ref-type="bibr" rid="ref2">2</xref><xref ref-type="bibr" rid="ref3">3</xref> This condition is usually asymptomatic, although it can be associated with burning, bleeding, an unpleasant taste. Even though several microorganisms can cause this condition, most studies showed that <italic>Candida albicans</italic> and the ill fitting dentures promote the development of this condition. <italic>Candida albicans</italic> is an oral commensally fungus found in 40% of human beings, which facilitates the formation of denture plaque, in which <italic>Candida albicans</italic><xref ref-type="bibr" rid="ref4">4</xref>–<xref ref-type="bibr" rid="ref7">7</xref> is commonly isolated as the pathogenic agents. The adhesion of <italic>Candida albicans</italic> to denture base material<xref ref-type="bibr" rid="ref8">8</xref>–<xref ref-type="bibr" rid="ref10">10</xref> particularly when worn continuously in the conditions of trauma and poor oral hygiene can usually cause denture stomatitis.<xref ref-type="bibr" rid="ref9">9</xref> One of the initial steps in prevention and treatment of denture stomatitis is improving denture adaptation and preparing the conditions for recovering of denture bearing tissues. Although tissue conditioners can improve the adaptation of the denture,<xref ref-type="bibr" rid="ref10">10</xref> they are susceptible to the attachment and colonization of oral microorganisms<xref ref-type="bibr" rid="ref11">11</xref><xref ref-type="bibr" rid="ref12">12</xref> especially <italic>Candida</italic> and resulted in the irritation of underlying tissues.<xref ref-type="bibr" rid="ref13">13</xref> Formation of <italic>Candida</italic> biofilm is the result of <italic>Candida</italic> colonization on tissue conditioner surfaces in an aqueous environment<xref ref-type="bibr" rid="ref14">14</xref> following a sequence of events including microbial surface attachment, cell proliferation, matrix production and detachment.<xref ref-type="bibr" rid="ref15">15</xref> Therefore, the prevention of biofilm formation is important for controlling denture stomatitis in denture wearers. Since the use of antifungal agents for prevention and treatment of denture stomatitis caused undesirable taste and also needed frequency doses; it was frequently associated with poor patient compliance.<xref ref-type="bibr" rid="ref13">13</xref> Several studies have attempted to incorporate antifungal agents to tissue conditioner for prevention of plaque formation and treatment of denture stomatitis.<xref ref-type="bibr" rid="ref16">16</xref><xref ref-type="bibr" rid="ref17">17</xref> The purpose of the current <italic>in vitro</italic> study was to evaluate the effectiveness of the combination of two common antifungal agents with tissue conditioner to inhibit the attachment and colonization of <italic>Candida albicans</italic>.</p></sec><sec sec-type="materials|methods" id="sec1-2"><title>Materials and Methods</title><sec id="sec2-1"><title>Candida albicans inoculation preparation</title><p>Clinical isolates of <italic>Candida albicans</italic> (ATCC 10231), obtained from the Department of molecular microbiology in Manchester, UK, were used as test organisms for the current experimental study. <italic>Candida albicans</italic> was cultured onto Sabouraud dextrose agar (Oxoid, UK) plate and incubated at 37ºC for 3 days. A colony from the stock culture was then diluted in 2 ml sterile saline and a suspension of 1×10<sup>6</sup> CFU/ml was prepared. Viable counts were performed to confirm the inoculums size. The number of colonies that appeared after incubation was expressed as CFUs (ColonyForming Units) and was enumerated with a Haemacytometer slide. The solution was used for further inoculums concentrations.</p></sec><sec id="sec2-2"><title>Incorporation of antifungals into tissue conditioner</title><p>Tissue conditioner, (Acropars, Tehran, Iran) was mixed and prepared according to manufacturer's instruction. Antifungal agents, nystatin (Iran Darou, Iran) and fluconazole (Pars Darou, Iran) were mixed into tissue conditioner powder at concentrations of 1, 3, 5, 10% wt/wt in a sterile plate. A sterile glass rod was used to prepare a thin film of tissue conditioner with 1mm thickness and punched as 5mm diameter disks. (<xref ref-type="fig" rid="F1">Figure 1</xref>) (N = 8). Eight specimens of pure tissue conditioner were also prepared as negative control.</p><fig id="F1" position="float"><label>Figure 1</label><caption><p>Tissue conditioner disks preparation for in vitro biofilm formation</p></caption><graphic xlink:href="DRJ-7-18-g001"></graphic></fig></sec><sec id="sec2-3"><title>Susceptibility testing</title><p>Broth microdilution testing<xref ref-type="bibr" rid="ref18">18</xref> was performed in 24 well sterile plastic cell culture microtiter plates), with their corresponding covers as follows:</p><p>Tissue conditioner disks (containing different concentration of antifungal agents and negative pure disks as control group) were immersed in triplicate in microtiter well containing 900 μl Sabouraud broths (Highmedia, Iran). All disks were contaminated with 100 μl of 1 × 10 <sup>6</sup> CFU/ml C. albicans cell suspension and the cell culture plate were incubated at 35ºC on a rotary shaker (150 rpm) for 48 hours. After incubation, the broth was removed with a sterile pipette. The specimens were rinsed 5 times with sterile water to remove the loosely adherent <italic>C. albicans.</italic></p><p>The specimens were placed in sterile test tubes that contained sterile saline and sonicated for 5 minutes to remove surface organisms. Tenfold serial dilution of the elution suspension were made, and 100 μl of each elute was placed on duplicate Sabouraud's agar plates. The plates were incubated at 37ºC for 48 hours and the colonies were enumerated.</p></sec><sec id="sec2-4"><title>Statistical tests</title><p>The groups were compared by KruskalWallis statistical test. Nonparametric multiple comparative test (Dunn's test) was also used for each test conditions using Prism software. The significant level was set at P = 0.05.</p></sec></sec><sec sec-type="results" id="sec1-3"><title>Results</title><p>Nystatin in all concentrations completely inhibited the attachment and colonization of <italic>C. albicans</italic> (P = 0.0001), but in the case of fluconazole, only the 10% wt/wt combination showed complete inhibition of <italic>Candida</italic> colonization while other concentrations reduced the density of <italic>Candida</italic> cells on the tissue conditioner disks. The controls were easily colonized by <italic>C. albicans</italic>. (<xref ref-type="table" rid="T1">Table 1</xref>)</p><table-wrap id="T1" position="float"><label>Table 1</label><caption><p>Candida density (CFU/ml) in the specimens treated with nystatin, fluconazole and in the control group</p></caption><graphic xlink:href="DRJ-7-18-g002"></graphic></table-wrap></sec><sec sec-type="discussion" id="sec1-4"><title>Discussion</title><p>Most tissue conditioners not only exhibit any antifungal activity, but different studies showed their supporting role in adhesion and growth of <italic>Candida</italic> leading to denture stomatitis in the oral cavity of denture wearers.<xref ref-type="bibr" rid="ref11">11</xref><xref ref-type="bibr" rid="ref12">12</xref> A tissue conditioner with antifungal activity could be a great advantage for patients with a high risk of denture stomatitis.<xref ref-type="bibr" rid="ref19">19</xref> Several attempts have been made to incorporate additives<xref ref-type="bibr" rid="ref17">17</xref><xref ref-type="bibr" rid="ref18">18</xref> and antifungals<xref ref-type="bibr" rid="ref16">16</xref><xref ref-type="bibr" rid="ref20">20</xref><xref ref-type="bibr" rid="ref21">21</xref> to tissue conditioner as a drug delivery method for controlling microbial attachment and colonization.</p><p>A number of studies placed agar on top of mixed concentrations of antifungal agents and tissue conditioners to evaluate the efficacy of this mixture by measuring the area of growth inhibition of <italic>C.albicans</italic>.<xref ref-type="bibr" rid="ref16">16</xref><xref ref-type="bibr" rid="ref20">20</xref><xref ref-type="bibr" rid="ref22">22</xref> Different antifungals such as nystatin, amphotericin B,<xref ref-type="bibr" rid="ref23">23</xref><xref ref-type="bibr" rid="ref24">24</xref> miconazole and ketoconazole<xref ref-type="bibr" rid="ref16">16</xref> combined with different tissue conditioners like Viscogel, Lynal, GC liner and Co comfort have been tested in this manner. Except for Amphotericin B and Viscogel, all other combinations showed a range of growth inhibition results which compared to that of the control group. Addition of herbal medicine to tissue conditioner was also studied for <italic>in vitro</italic> and <italic>in vivo</italic> controlling of denture stomatitis.<xref ref-type="bibr" rid="ref18">18</xref></p><p>However, comparing results from different studies was difficult due to the lack of standardization of concentrations used.<xref ref-type="bibr" rid="ref25">25</xref> Since the addition of antifungal agents into denture liners did not alter the mechanical properties of tissue conditioners,<xref ref-type="bibr" rid="ref26">26</xref><xref ref-type="bibr" rid="ref27">27</xref> in this study the broth dilution method was used to investigate the fungicidal effects of antifungal agents incorporated into tissue conditioners. In this method, <italic>C. albicans</italic> suspensions were prepared from a log phase colony and used for inoculation of the test and control groups. The present method varied from previous studies in that the goal was to record the fungicidal activity more accurately based on the Clinical and Laboratory Standards Institute (CLSI) method rather than the agar assays used in previous studies.<xref ref-type="bibr" rid="ref19">19</xref> Study by Saatchi showed that Acropars tissue conditioner has no antifungal effects by itself.<xref ref-type="bibr" rid="ref28">28</xref></p><p>An oral suspension of both antifungal agents was used in this study (<xref ref-type="table" rid="T2">Table 2</xref>). <xref ref-type="bibr" rid="ref29">29</xref> Nystatin is the current standard topical treatment for oral candidiasis and fluconazole is the most common systemic antifungal used alternative to topical nystatin<xref ref-type="bibr" rid="ref7">7</xref>.</p><table-wrap id="T2" position="float"><label>Table 2</label><caption><p>Recommended dosage of antifungal agents<xref ref-type="bibr" rid="ref29">29</xref></p></caption><graphic xlink:href="DRJ-7-18-g003"></graphic></table-wrap><p>In the present study nystatin showed higher inhibitory effects than fluconazole as in all tested combinations completely inhibited the production of <italic>C. albicans</italic> in tissue conditioner disks, however only 10% fluconazole could completely prevent the growth and adhesion of <italic>Candida</italic>. Combination of 3% and 5% fluconazole to Acrosoft were also showed to have significant inhibitory properties compared to control. Chow et al.<xref ref-type="bibr" rid="ref25">25</xref> incorporated nystatin, fluconazole and itraconazole into tissue conditioners to investigate their drug delivery effectiveness using conventional agar based method by measuring their inhibition activities. They showed that itraconazole and fluconazole have more inhibitory activity than nystatin, which is different from the results of our study. The broth microdilution technique used this study was economical and less timeconsuming than agar based methods and was able to eliminate uncontrolled results as it facilitated data analysis.<xref ref-type="bibr" rid="ref19">19</xref></p></sec><sec sec-type="conclusion" id="sec1-5"><title>Conclusion</title><p>Incorporation of nystatin rather than fluconazole into tissue conditioners is more effective in treatment of chronic atrophic candidiasis in denture users. Combination of 1% wt/wt nystatin with Acrosoft is recommended for treating Candidal infections under dentures.</p></sec></body><back><ack><p>This work has been supported by Yazd University of Medical Sciences and Health Services (thesis number 553).The authors would like to thank Farzaneh Mirzaei for her kind help in the laboratory works.</p></ack><ref-list><title>References</title><ref id="ref1"><label>1</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wilson</surname><given-names>J</given-names></name></person-group><article-title>The aetiology, diagnosis and management of denture stomatitis</article-title><source>Br Dent J</source><year>1998</year><volume>185</volume><issue>8</issue><fpage>380</fpage><lpage>4</lpage><pub-id pub-id-type="pmid">9828496</pub-id></element-citation></ref><ref id="ref2"><label>2</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Budtz-Jlrgensen</surname><given-names>E</given-names></name><name><surname>Mojon</surname><given-names>P</given-names></name><name><surname>Banon-Clement</surname><given-names>JM</given-names></name><name><surname>Baehni</surname><given-names>P</given-names></name></person-group><article-title>Oral candidosis in long-term hospital care: comparison of edentulous and dentate subjects</article-title><source>Oral Dis</source><year>1996</year><volume>2</volume><issue>4</issue><fpage>285</fpage><lpage>90</lpage><pub-id pub-id-type="pmid">9171512</pub-id></element-citation></ref><ref id="ref3"><label>3</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Espinoza</surname><given-names>I</given-names></name><name><surname>Rojas</surname><given-names>R</given-names></name><name><surname>Aranda</surname><given-names>W</given-names></name><name><surname>Gamonal</surname><given-names>J</given-names></name></person-group><article-title>Prevalence of oral mucosal lesions in elderly people in Santiago, Chile</article-title><source>J Oral Pathol Med</source><year>2003</year><volume>32</volume><issue>10</issue><fpage>571</fpage><lpage>5</lpage><pub-id pub-id-type="pmid">14632931</pub-id></element-citation></ref><ref id="ref4"><label>4</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Darwazeh</surname><given-names>AM</given-names></name><name><surname>Al Refai</surname><given-names>S</given-names></name><name><surname>Al Mojaiwel</surname><given-names>S</given-names></name></person-group><article-title>Isolation of Candida species from the oral cavity and finger-tips of complete denture wearers</article-title><source>J Prosthet Dent</source><year>2001</year><volume>86</volume><issue>4</issue><fpage>420</fpage><lpage>3</lpage><pub-id pub-id-type="pmid">11677537</pub-id></element-citation></ref><ref id="ref5"><label>5</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Barbeau</surname><given-names>J</given-names></name><name><surname>Seguin</surname><given-names>J</given-names></name><name><surname>Goulet</surname><given-names>JP</given-names></name><name><surname>de Koninck</surname><given-names>L</given-names></name><name><surname>Avon</surname><given-names>SL</given-names></name><name><surname>Lalonde</surname><given-names>B</given-names></name><etal></etal></person-group><article-title>Reassessing the presence of Candida albicans in denture-related stomatitis</article-title><source>Oral Surg Oral Med Oral Pathol Oral Radiol Endod</source><year>2003</year><volume>95</volume><issue>1</issue><fpage>51</fpage><lpage>9</lpage><pub-id pub-id-type="pmid">12539027</pub-id></element-citation></ref><ref id="ref6"><label>6</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Monsenego</surname><given-names>P</given-names></name></person-group><article-title>Presence of microorganisms on the fitting denture complete surface: study ‘in vivo’</article-title><source>J Oral Rehabil</source><year>2000</year><volume>27</volume><issue>8</issue><fpage>708</fpage><lpage>13</lpage><pub-id pub-id-type="pmid">10931267</pub-id></element-citation></ref><ref id="ref7"><label>7</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Bissell</surname><given-names>V</given-names></name><name><surname>Felix</surname><given-names>DH</given-names></name><name><surname>Wray</surname><given-names>D</given-names></name></person-group><article-title>Comparative trial of fluconazole and amphotericin in the treatment of denture stomatitis</article-title><source>Oral Surg Oral Med Oral Pathol</source><year>1993</year><volume>76</volume><issue>1</issue><fpage>35</fpage><lpage>9</lpage><pub-id pub-id-type="pmid">8351118</pub-id></element-citation></ref><ref id="ref8"><label>8</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Moura</surname><given-names>JS</given-names></name><name><surname>da Silva</surname><given-names>WJ</given-names></name><name><surname>Pereira</surname><given-names>T</given-names></name><name><surname>Bel Cury</surname><given-names>AA</given-names></name><name><surname>Rodrigues Garcia</surname><given-names>RC</given-names></name></person-group><article-title>Influence of acrylic resin polymerization methods and saliva on the adherence of four Candida species</article-title><source>J Prosthet Dent</source><year>2006</year><volume>96</volume><issue>3</issue><fpage>205</fpage><lpage>11</lpage><pub-id pub-id-type="pmid">16990072</pub-id></element-citation></ref><ref id="ref9"><label>9</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Fanello</surname><given-names>S</given-names></name><name><surname>Bouchara</surname><given-names>JP</given-names></name><name><surname>Sauteron</surname><given-names>M</given-names></name><name><surname>Delbos</surname><given-names>V</given-names></name><name><surname>Parot</surname><given-names>E</given-names></name><name><surname>Marot-Leblond</surname><given-names>A</given-names></name><etal></etal></person-group><article-title>Predictive value of oral colonization by Candida yeasts for the onset of a nosocomial infection in elderly hospitalized patients</article-title><source>J Med Microbiol</source><year>2006</year><volume>55</volume><issue>Pt 2</issue><fpage>223</fpage><lpage>8</lpage><pub-id pub-id-type="pmid">16434716</pub-id></element-citation></ref><ref id="ref10"><label>10</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chander</surname><given-names>S</given-names></name><name><surname>Hill</surname><given-names>M</given-names></name><name><surname>Moore</surname><given-names>D</given-names></name><name><surname>Morrow</surname><given-names>L</given-names></name></person-group><article-title>Tissue conditioning materials as functional impression materials</article-title><source>Eur J Prosthodont Restor Dent</source><year>2007</year><volume>15</volume><issue>2</issue><fpage>67</fpage><lpage>71</lpage><pub-id pub-id-type="pmid">17645067</pub-id></element-citation></ref><ref id="ref11"><label>11</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Waters</surname><given-names>MG</given-names></name><name><surname>Williams</surname><given-names>DW</given-names></name><name><surname>Jagger</surname><given-names>RG</given-names></name><name><surname>Lewis</surname><given-names>MA</given-names></name></person-group><article-title>Adherence of Candida albicans to experimental denture soft lining materials</article-title><source>J Prosthet Dent</source><year>1997</year><volume>77</volume><issue>3</issue><fpage>306</fpage><lpage>12</lpage><pub-id pub-id-type="pmid">9069086</pub-id></element-citation></ref><ref id="ref12"><label>12</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kulak</surname><given-names>Y</given-names></name><name><surname>Kadir</surname><given-names>T</given-names></name></person-group><article-title>in vitro study of fungal presence and growth on three tissue conditioner materials</article-title><source>J Marmara Univ Dent Fac</source><year>1997</year><volume>2</volume><issue>4</issue><fpage>682</fpage><lpage>4</lpage><pub-id pub-id-type="pmid">9569798</pub-id></element-citation></ref><ref id="ref13"><label>13</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ramage</surname><given-names>G</given-names></name><name><surname>Vandewalle</surname><given-names>K</given-names></name><name><surname>Wickes</surname><given-names>BL</given-names></name><name><surname>Lopez-Ribot</surname><given-names>JL</given-names></name></person-group><article-title>Characteristics of biofilm formation by Candida albicans</article-title><source>Rev Iberoam Micol</source><year>2001</year><volume>18</volume><issue>4</issue><fpage>163</fpage><lpage>70</lpage><pub-id pub-id-type="pmid">15496122</pub-id></element-citation></ref><ref id="ref14"><label>14</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Weir</surname><given-names>E</given-names></name><name><surname>Lawlor</surname><given-names>A</given-names></name><name><surname>Whelan</surname><given-names>A</given-names></name><name><surname>Regan</surname><given-names>F</given-names></name></person-group><article-title>The use of nanoparticles in anti-microbial materials and their characterization</article-title><source>Analyst</source><year>2008</year><volume>133</volume><issue>7</issue><fpage>835</fpage><lpage>45</lpage><pub-id pub-id-type="pmid">18575632</pub-id></element-citation></ref><ref id="ref15"><label>15</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Valappil</surname><given-names>SP</given-names></name><name><surname>Pickup</surname><given-names>DM</given-names></name><name><surname>Carroll</surname><given-names>DL</given-names></name><name><surname>Hope</surname><given-names>CK</given-names></name><name><surname>Pratten</surname><given-names>J</given-names></name><name><surname>Newport</surname><given-names>RJ</given-names></name><etal></etal></person-group><article-title>Effect of silver content on the structure and antibacterial activity of silver-doped phosphate-based glasses</article-title><source>Antimicrob Agents Chemother</source><year>2007</year><volume>51</volume><issue>12</issue><fpage>4453</fpage><lpage>61</lpage><pub-id pub-id-type="pmid">17908949</pub-id></element-citation></ref><ref id="ref16"><label>16</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Quinn</surname><given-names>DM</given-names></name></person-group><article-title>The effectiveness, in vitro, of micona-zole and ketoconazole combined with tissue condi-tioners in inhibiting the growth of Candida albicans</article-title><source>J Oral Rehabil</source><year>1985</year><volume>12</volume><issue>2</issue><fpage>177</fpage><lpage>82</lpage><pub-id pub-id-type="pmid">3886866</pub-id></element-citation></ref><ref id="ref17"><label>17</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Akiba</surname><given-names>N</given-names></name><name><surname>Hayakawa</surname><given-names>I</given-names></name><name><surname>Keh</surname><given-names>ES</given-names></name><name><surname>Watanabe</surname><given-names>A</given-names></name></person-group><article-title>Anti-fungal effects of a tissue conditioner coating agent with TiO2 photocatalyst</article-title><source>J Med Dent Sci</source><year>2005</year><volume>52</volume><issue>4</issue><fpage>223</fpage><lpage>7</lpage><pub-id pub-id-type="pmid">16669456</pub-id></element-citation></ref><ref id="ref18"><label>18</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Catalan</surname><given-names>A</given-names></name><name><surname>Pacheco</surname><given-names>JG</given-names></name><name><surname>Martinez</surname><given-names>A</given-names></name><name><surname>Mondaca</surname><given-names>MA</given-names></name></person-group><article-title>In vitro and in vivo activity of Melaleuca alternifolia mixed with tissue conditioner on Candida albicans</article-title><source>Oral Surg Oral Med Oral Pathol Oral Radiol Endod</source><year>2008</year><volume>105</volume><issue>3</issue><fpage>327</fpage><lpage>32</lpage><pub-id pub-id-type="pmid">18280967</pub-id></element-citation></ref><ref id="ref19"><label>19</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Espinel-Ingroff</surname><given-names>A</given-names></name><name><surname>Fothergill</surname><given-names>A</given-names></name><name><surname>Ghannoum</surname><given-names>M</given-names></name><name><surname>Manavathu</surname><given-names>E</given-names></name><name><surname>Ostrosky-Zeichner</surname><given-names>L</given-names></name><name><surname>Pfaller</surname><given-names>M</given-names></name><etal></etal></person-group><article-title>Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M 38-A document) for amphotericin B, itraconazole, posa-conazole, and voriconazole</article-title><source>J Clin Microbiol</source><year>2005</year><volume>43</volume><issue>10</issue><fpage>5243</fpage><lpage>6</lpage><pub-id pub-id-type="pmid">16207990</pub-id></element-citation></ref><ref id="ref20"><label>20</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Geerts</surname><given-names>GA</given-names></name><name><surname>Stuhlinger</surname><given-names>ME</given-names></name><name><surname>Basson</surname><given-names>NJ</given-names></name></person-group><article-title>Effect of an antifungal denture liner on the saliva yeast count in patients with denture stomatitis: a pilot study</article-title><source>J Oral Rehabil</source><year>2008</year><volume>35</volume><issue>9</issue><fpage>664</fpage><lpage>9</lpage><pub-id pub-id-type="pmid">18793352</pub-id></element-citation></ref><ref id="ref21"><label>21</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jittapiromsak</surname><given-names>N</given-names></name><name><surname>Benjavongkulchai</surname><given-names>E</given-names></name><name><surname>Purnaveja</surname><given-names>S</given-names></name></person-group><article-title>Nystatin Release from Tissue Conditioner and Its Candidicidal Effects</article-title><source>Clinical Microbiology Poster</source><year>2004</year><fpage>10</fpage><lpage>3</lpage></element-citation></ref><ref id="ref22"><label>22</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Rathore</surname><given-names>P</given-names></name><name><surname>Hegde</surname><given-names>A</given-names></name><name><surname>Ginjupalli</surname><given-names>K</given-names></name><name><surname>Upadhya</surname><given-names>N</given-names></name></person-group><article-title>Evaluation of antifungal activity of additives to resi-lient liners: an <italic>in vitro</italic> pilot study</article-title><source>Trends in Bioma-terials and Artificial Organs</source><year>2009</year><volume>23</volume><issue>1</issue><fpage>6</fpage><lpage>9</lpage></element-citation></ref><ref id="ref23"><label>23</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Truhlar</surname><given-names>MR</given-names></name><name><surname>Shay</surname><given-names>K</given-names></name><name><surname>Sohnle</surname><given-names>P</given-names></name></person-group><article-title>Use of a new assay technique for quantification of antifungal activity of nystatin incorporated in denture liners</article-title><source>J Prosthet Dent</source><year>1994</year><volume>71</volume><issue>5</issue><fpage>517</fpage><lpage>24</lpage><pub-id pub-id-type="pmid">8006850</pub-id></element-citation></ref><ref id="ref24"><label>24</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Thomas</surname><given-names>CJ</given-names></name><name><surname>Nutt</surname><given-names>GM</given-names></name></person-group><article-title>The <italic>in vitro</italic> fungicidal prop-erties of Visco-gel, alone and combined with nysta-tin and amphotericin B</article-title><source>J Oral Rehabil</source><year>1978</year><volume>5</volume><issue>2</issue><fpage>167</fpage><lpage>72</lpage><pub-id pub-id-type="pmid">361931</pub-id></element-citation></ref><ref id="ref25"><label>25</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chow</surname><given-names>CK</given-names></name><name><surname>Matear</surname><given-names>DW</given-names></name><name><surname>Lawrence</surname><given-names>HP</given-names></name></person-group><article-title>Efficacy of antifungal agents in tissue conditioners in treating candidiasis</article-title><source>Gerodontology</source><year>1999</year><volume>16</volume><issue>2</issue><fpage>110</fpage><lpage>8</lpage><pub-id pub-id-type="pmid">10825850</pub-id></element-citation></ref><ref id="ref26"><label>26</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Dogan</surname><given-names>A</given-names></name><name><surname>Bek</surname><given-names>B</given-names></name><name><surname>Cevik</surname><given-names>NN</given-names></name><name><surname>Usanmaz</surname><given-names>A</given-names></name></person-group><article-title>The effect of preparation conditions of acrylic denture base materials on the level of residual monomer, mechanical properties and water absorption</article-title><source>J Dent</source><year>1995</year><volume>23</volume><issue>5</issue><fpage>313</fpage><lpage>8</lpage><pub-id pub-id-type="pmid">7560378</pub-id></element-citation></ref><ref id="ref27"><label>27</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Douglas</surname><given-names>WH</given-names></name><name><surname>Clarke</surname><given-names>DA</given-names></name></person-group><article-title>Physical and mechanical properties of nystatin-containing denture liners</article-title><source>J Prosthet Dent</source><year>1975</year><volume>34</volume><issue>4</issue><fpage>428</fpage><lpage>34</lpage><pub-id pub-id-type="pmid">1100810</pub-id></element-citation></ref><ref id="ref28"><label>28</label><element-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Saatchi</surname><given-names>S</given-names></name></person-group><article-title>Comarative study of the effect of two kinds of tissue conditioner materials on the oral candida growth in patients with maxillary complete denture</article-title><source>Journal of Hamadan University of Medical Sciences and Health Services</source><year>2003</year><volume>10</volume><issue>3</issue><fpage>21</fpage><lpage>4</lpage></element-citation></ref><ref id="ref29"><label>29</label><element-citation publication-type="book"><person-group person-group-type="author"><name><surname>Martindale</surname><given-names>W</given-names></name></person-group><article-title>The Extra pharmacopoeia</article-title><year>2001</year><edition>34th ed</edition><publisher-loc>London</publisher-loc><publisher-name>Pharmaceutical Press</publisher-name><fpage>123</fpage></element-citation></ref></ref-list></back></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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