Candidate salivary biomarkers associated with alveolar bone loss: cross-sectional and in vitro studies
Identifieur interne : 002782 ( Pmc/Corpus ); précédent : 002781; suivant : 002783Candidate salivary biomarkers associated with alveolar bone loss: cross-sectional and in vitro studies
Auteurs : Patricia Yen Bee Ng ; Maureen Donley ; Ernest Hausmann ; Alan D. Hutson ; Edward F. Rossomando ; Frank A. ScannapiecoSource :
- FEMS immunology and medical microbiology [ 0928-8244 ] ; 2007.
Abstract
This cross-sectional study evaluated the association between radiographic evidence of alveolar bone loss and the concentration of host-derived bone resorptive factors (interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6, prostaglandin-E2), and markers of bone turnover [pyridinoline cross-linked carboxyterminal telopeptide of type I collagen (ICTP), osteocalcin, osteonectin] in stimulated human whole saliva collected from 110 untreated dental patients. Alveolar bone loss scores for each patient were derived from radiographic examination. Variables positively associated with increased bone loss score were: age, current smoking, use of bisphosphonate drugs, and salivary interleukin-1beta levels above the median. Salivary osteonectin levels above the median were associated with a decreased bone loss score. Additional
Url:
DOI: 10.1111/j.1574-695X.2006.00187.x
PubMed: 17328758
PubMed Central: 2258090
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PMC:2258090Le document en format XML
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<author><name sortKey="Ng, Patricia Yen Bee" sort="Ng, Patricia Yen Bee" uniqKey="Ng P" first="Patricia Yen Bee" last="Ng">Patricia Yen Bee Ng</name>
<affiliation><nlm:aff id="A1">Department of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, Buffalo, NY, USA</nlm:aff>
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<author><name sortKey="Donley, Maureen" sort="Donley, Maureen" uniqKey="Donley M" first="Maureen" last="Donley">Maureen Donley</name>
<affiliation><nlm:aff id="A2">Department of Oral Diagnostic Sciences, School of Dental Medicine, University at Buffalo, Buffalo, NY, USA</nlm:aff>
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<author><name sortKey="Hausmann, Ernest" sort="Hausmann, Ernest" uniqKey="Hausmann E" first="Ernest" last="Hausmann">Ernest Hausmann</name>
<affiliation><nlm:aff id="A1">Department of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, Buffalo, NY, USA</nlm:aff>
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<author><name sortKey="Hutson, Alan D" sort="Hutson, Alan D" uniqKey="Hutson A" first="Alan D." last="Hutson">Alan D. Hutson</name>
<affiliation><nlm:aff id="A3">Department of Biostatistics, University at Buffalo, NY, USA</nlm:aff>
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<author><name sortKey="Rossomando, Edward F" sort="Rossomando, Edward F" uniqKey="Rossomando E" first="Edward F." last="Rossomando">Edward F. Rossomando</name>
<affiliation><nlm:aff id="A4">Department of BioStructure and Function, University of Connecticut Health Center, Farmington, CT, USA</nlm:aff>
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<author><name sortKey="Scannapieco, Frank A" sort="Scannapieco, Frank A" uniqKey="Scannapieco F" first="Frank A." last="Scannapieco">Frank A. Scannapieco</name>
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<author><name sortKey="Donley, Maureen" sort="Donley, Maureen" uniqKey="Donley M" first="Maureen" last="Donley">Maureen Donley</name>
<affiliation><nlm:aff id="A2">Department of Oral Diagnostic Sciences, School of Dental Medicine, University at Buffalo, Buffalo, NY, USA</nlm:aff>
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<author><name sortKey="Hausmann, Ernest" sort="Hausmann, Ernest" uniqKey="Hausmann E" first="Ernest" last="Hausmann">Ernest Hausmann</name>
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<author><name sortKey="Hutson, Alan D" sort="Hutson, Alan D" uniqKey="Hutson A" first="Alan D." last="Hutson">Alan D. Hutson</name>
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<author><name sortKey="Rossomando, Edward F" sort="Rossomando, Edward F" uniqKey="Rossomando E" first="Edward F." last="Rossomando">Edward F. Rossomando</name>
<affiliation><nlm:aff id="A4">Department of BioStructure and Function, University of Connecticut Health Center, Farmington, CT, USA</nlm:aff>
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<author><name sortKey="Scannapieco, Frank A" sort="Scannapieco, Frank A" uniqKey="Scannapieco F" first="Frank A." last="Scannapieco">Frank A. Scannapieco</name>
<affiliation><nlm:aff id="A1">Department of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, Buffalo, NY, USA</nlm:aff>
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<series><title level="j">FEMS immunology and medical microbiology</title>
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<front><div type="abstract" xml:lang="en"><p id="P1">This cross-sectional study evaluated the association between radiographic evidence of alveolar bone loss and the concentration of host-derived bone resorptive factors (interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6, prostaglandin-E2), and markers of bone turnover [pyridinoline cross-linked carboxyterminal telopeptide of type I collagen (ICTP), osteocalcin, osteonectin] in stimulated human whole saliva collected from 110 untreated dental patients. Alveolar bone loss scores for each patient were derived from radiographic examination. Variables positively associated with increased bone loss score were: age, current smoking, use of bisphosphonate drugs, and salivary interleukin-1beta levels above the median. Salivary osteonectin levels above the median were associated with a decreased bone loss score. Additional <italic>in vitro</italic>
studies were carried out to determine the fate of interleukin-1beta, interleukin-6 and tumor necrosis factor-alpha added to whole and parotid saliva. All cytokines added to saliva were detected in significantly lower concentrations than when added to buffer alone. Protease inhibitors added to saliva did not prevent the reduction in detection of biomarkers. Variation in time of incubation, repeated cycles of freezing and thawing, or exposure to dimethylsulfoxide did not appreciably affect the measurement of cytokines in saliva. These results suggest that detection of biomarkers by conventional immunoassays may underestimate the actual quantity of molecules in saliva.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">9315554</journal-id>
<journal-id journal-id-type="pubmed-jr-id">2344</journal-id>
<journal-id journal-id-type="nlm-ta">FEMS Immunol Med Microbiol</journal-id>
<journal-title>FEMS immunology and medical microbiology</journal-title>
<issn pub-type="ppub">0928-8244</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">17328758</article-id>
<article-id pub-id-type="pmc">2258090</article-id>
<article-id pub-id-type="doi">10.1111/j.1574-695X.2006.00187.x</article-id>
<article-id pub-id-type="manuscript">NIHMS39076</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Candidate salivary biomarkers associated with alveolar bone loss: cross-sectional and <italic>in vitro</italic>
studies</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Ng</surname>
<given-names>Patricia Yen Bee</given-names>
</name>
<xref rid="A1" ref-type="aff">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Donley</surname>
<given-names>Maureen</given-names>
</name>
<xref rid="A2" ref-type="aff">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hausmann</surname>
<given-names>Ernest</given-names>
</name>
<xref rid="A1" ref-type="aff">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hutson</surname>
<given-names>Alan D.</given-names>
</name>
<xref rid="A3" ref-type="aff">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Rossomando</surname>
<given-names>Edward F.</given-names>
</name>
<xref rid="A4" ref-type="aff">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Scannapieco</surname>
<given-names>Frank A.</given-names>
</name>
<xref rid="A1" ref-type="aff">1</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>1</label>
Department of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, Buffalo, NY, USA</aff>
<aff id="A2"><label>2</label>
Department of Oral Diagnostic Sciences, School of Dental Medicine, University at Buffalo, Buffalo, NY, USA</aff>
<aff id="A3"><label>3</label>
Department of Biostatistics, University at Buffalo, NY, USA</aff>
<aff id="A4"><label>4</label>
Department of BioStructure and Function, University of Connecticut Health Center, Farmington, CT, USA</aff>
<author-notes><corresp id="FN1">Correspondence: Frank A. Scannapieco, Department of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, 109 Foster Hall, Buffalo, NY 14214, USA. Tel.: +1 716 829 3373; fax: +1 716 829 3942; e-mail: <email>fas1@buffalo.edu</email>
</corresp>
<fn id="FN2"><p>Editor: Alex van Belkum</p>
</fn>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>20</day>
<month>2</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="ppub"><month>3</month>
<year>2007</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>28</day>
<month>2</month>
<year>2008</year>
</pub-date>
<volume>49</volume>
<issue>2</issue>
<fpage>252</fpage>
<lpage>260</lpage>
<abstract><p id="P1">This cross-sectional study evaluated the association between radiographic evidence of alveolar bone loss and the concentration of host-derived bone resorptive factors (interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6, prostaglandin-E2), and markers of bone turnover [pyridinoline cross-linked carboxyterminal telopeptide of type I collagen (ICTP), osteocalcin, osteonectin] in stimulated human whole saliva collected from 110 untreated dental patients. Alveolar bone loss scores for each patient were derived from radiographic examination. Variables positively associated with increased bone loss score were: age, current smoking, use of bisphosphonate drugs, and salivary interleukin-1beta levels above the median. Salivary osteonectin levels above the median were associated with a decreased bone loss score. Additional <italic>in vitro</italic>
studies were carried out to determine the fate of interleukin-1beta, interleukin-6 and tumor necrosis factor-alpha added to whole and parotid saliva. All cytokines added to saliva were detected in significantly lower concentrations than when added to buffer alone. Protease inhibitors added to saliva did not prevent the reduction in detection of biomarkers. Variation in time of incubation, repeated cycles of freezing and thawing, or exposure to dimethylsulfoxide did not appreciably affect the measurement of cytokines in saliva. These results suggest that detection of biomarkers by conventional immunoassays may underestimate the actual quantity of molecules in saliva.</p>
</abstract>
<kwd-group><kwd>periodontal disease</kwd>
<kwd>salivary biomarkers</kwd>
<kwd>pro-inflammatory cytokines</kwd>
<kwd>bone turnover</kwd>
</kwd-group>
<contract-num rid="DE1">R21 DE015854-02</contract-num>
<contract-sponsor id="DE1">National Institute of Dental and Craniofacial Research : NIDCR</contract-sponsor>
</article-meta>
</front>
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