Salivary Biomarkers in a Biofilm Overgrowth Model
Identifieur interne : 002703 ( Pmc/Corpus ); précédent : 002702; suivant : 002704Salivary Biomarkers in a Biofilm Overgrowth Model
Auteurs : Thiago Morelli ; Michael Stella ; Silvana P. Barros ; Julie T. Marchesan ; Kevin L. Moss ; Steven J. Kim ; Ning Yu ; Marcelo B. Aspiras ; Marilyn Ward ; Steven OffenbacherSource :
- Journal of periodontology [ 0022-3492 ] ; 2014.
Abstract
The purpose of this study was to determine whether baseline salivary inflammatory biomarkers could discriminate between different clinical levels of disease and/or predict clinical progression over a 3-week stent-induced biofilm overgrowth (SIBO) period.
168 participants were enrolled in a 21-day experimental gingivitis investigation and grouped according to clinical measures of periodontal status of health and diseased individuals representing each of five biofilm gingival interface (BGI) periodontal groups (H, G, P1, P2, P3). Stents were used to prevent plaque removal during brushing over one maxillary and one mandibular posterior dental sextant for 21 days. Clinical periodontal parameters and unstimulated saliva were collected at screening, baseline, and each week during SIBO. Saliva samples were assessed for levels of 13 different biomarkers by multiplex immunoassay.
Higher salivary levels of interleukin (IL)-1β, matrix metalloproteinase (MMP)-3, MMP-8, MMP-9, and neutrophil gelatinase-associated lipocalin (NGAL) were found in diseased groups compared to healthy at baseline. Conversely, higher IL-1 receptor antagonist (ra) levels were found in healthy patients at baseline. In addition, during SIBO MMP-1, tissue inhibitor of metalloproteinase (TIMP)-1, and TIMP-2 levels increased across all participant groups. A stepwise linear regression model using all salivary biomarkers demonstrated that at baseline increased IL-1ra (p=0.0044) and IL-6 (p=0.0093) were the two best predictors of change in probing depths during SIBO.
In summary, this investigation supports salivary levels of IL-1ra and IL-6 as potential indicators for significant probing depth changes during induced gingival inflammation. In addition, participants from BGI-P3 group (severe periodontitis) demonstrated elevated baseline levels of IL-1β, MMP-3, MMP-8, MMP-9, and NGAL compared to other study groups strengthening the relevance of participant's biological phenotype on salivary biomarkers expression
Url:
DOI: 10.1902/jop.2014.140180
PubMed: 25079398
PubMed Central: 4383599
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PMC:4383599Le document en format XML
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<author><name sortKey="Morelli, Thiago" sort="Morelli, Thiago" uniqKey="Morelli T" first="Thiago" last="Morelli">Thiago Morelli</name>
<affiliation><nlm:aff id="A1">Department of Periodontology, School of Dentistry, University of North Carolina at Chapel hill, Chapel Hill, NC, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Stella, Michael" sort="Stella, Michael" uniqKey="Stella M" first="Michael" last="Stella">Michael Stella</name>
<affiliation><nlm:aff id="A1">Department of Periodontology, School of Dentistry, University of North Carolina at Chapel hill, Chapel Hill, NC, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Barros, Silvana P" sort="Barros, Silvana P" uniqKey="Barros S" first="Silvana P." last="Barros">Silvana P. Barros</name>
<affiliation><nlm:aff id="A1">Department of Periodontology, School of Dentistry, University of North Carolina at Chapel hill, Chapel Hill, NC, USA</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A2">Center for Oral and Systemic Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA</nlm:aff>
</affiliation>
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<author><name sortKey="Marchesan, Julie T" sort="Marchesan, Julie T" uniqKey="Marchesan J" first="Julie T." last="Marchesan">Julie T. Marchesan</name>
<affiliation><nlm:aff id="A2">Center for Oral and Systemic Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA</nlm:aff>
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<author><name sortKey="Moss, Kevin L" sort="Moss, Kevin L" uniqKey="Moss K" first="Kevin L." last="Moss">Kevin L. Moss</name>
<affiliation><nlm:aff id="A2">Center for Oral and Systemic Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA</nlm:aff>
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<author><name sortKey="Kim, Steven J" sort="Kim, Steven J" uniqKey="Kim S" first="Steven J." last="Kim">Steven J. Kim</name>
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<author><name sortKey="Yu, Ning" sort="Yu, Ning" uniqKey="Yu N" first="Ning" last="Yu">Ning Yu</name>
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<author><name sortKey="Aspiras, Marcelo B" sort="Aspiras, Marcelo B" uniqKey="Aspiras M" first="Marcelo B." last="Aspiras">Marcelo B. Aspiras</name>
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<author><name sortKey="Ward, Marilyn" sort="Ward, Marilyn" uniqKey="Ward M" first="Marilyn" last="Ward">Marilyn Ward</name>
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<author><name sortKey="Offenbacher, Steven" sort="Offenbacher, Steven" uniqKey="Offenbacher S" first="Steven" last="Offenbacher">Steven Offenbacher</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Salivary Biomarkers in a Biofilm Overgrowth Model</title>
<author><name sortKey="Morelli, Thiago" sort="Morelli, Thiago" uniqKey="Morelli T" first="Thiago" last="Morelli">Thiago Morelli</name>
<affiliation><nlm:aff id="A1">Department of Periodontology, School of Dentistry, University of North Carolina at Chapel hill, Chapel Hill, NC, USA</nlm:aff>
</affiliation>
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<author><name sortKey="Stella, Michael" sort="Stella, Michael" uniqKey="Stella M" first="Michael" last="Stella">Michael Stella</name>
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</affiliation>
</author>
<author><name sortKey="Barros, Silvana P" sort="Barros, Silvana P" uniqKey="Barros S" first="Silvana P." last="Barros">Silvana P. Barros</name>
<affiliation><nlm:aff id="A1">Department of Periodontology, School of Dentistry, University of North Carolina at Chapel hill, Chapel Hill, NC, USA</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A2">Center for Oral and Systemic Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA</nlm:aff>
</affiliation>
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<author><name sortKey="Marchesan, Julie T" sort="Marchesan, Julie T" uniqKey="Marchesan J" first="Julie T." last="Marchesan">Julie T. Marchesan</name>
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</affiliation>
</author>
<author><name sortKey="Moss, Kevin L" sort="Moss, Kevin L" uniqKey="Moss K" first="Kevin L." last="Moss">Kevin L. Moss</name>
<affiliation><nlm:aff id="A2">Center for Oral and Systemic Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kim, Steven J" sort="Kim, Steven J" uniqKey="Kim S" first="Steven J." last="Kim">Steven J. Kim</name>
<affiliation><nlm:aff id="A2">Center for Oral and Systemic Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yu, Ning" sort="Yu, Ning" uniqKey="Yu N" first="Ning" last="Yu">Ning Yu</name>
<affiliation><nlm:aff id="A2">Center for Oral and Systemic Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Aspiras, Marcelo B" sort="Aspiras, Marcelo B" uniqKey="Aspiras M" first="Marcelo B." last="Aspiras">Marcelo B. Aspiras</name>
<affiliation><nlm:aff id="A3">Philips Oral Healthcare - Dental & Scientific Affairs, Bothell, WA, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ward, Marilyn" sort="Ward, Marilyn" uniqKey="Ward M" first="Marilyn" last="Ward">Marilyn Ward</name>
<affiliation><nlm:aff id="A3">Philips Oral Healthcare - Dental & Scientific Affairs, Bothell, WA, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Offenbacher, Steven" sort="Offenbacher, Steven" uniqKey="Offenbacher S" first="Steven" last="Offenbacher">Steven Offenbacher</name>
<affiliation><nlm:aff id="A1">Department of Periodontology, School of Dentistry, University of North Carolina at Chapel hill, Chapel Hill, NC, USA</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A2">Center for Oral and Systemic Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA</nlm:aff>
</affiliation>
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<series><title level="j">Journal of periodontology</title>
<idno type="ISSN">0022-3492</idno>
<idno type="eISSN">1943-3670</idno>
<imprint><date when="2014">2014</date>
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<front><div type="abstract" xml:lang="en"><sec id="S1"><title>Background</title>
<p id="P1">The purpose of this study was to determine whether baseline salivary inflammatory biomarkers could discriminate between different clinical levels of disease and/or predict clinical progression over a 3-week stent-induced biofilm overgrowth (SIBO) period.</p>
</sec>
<sec id="S2"><title>Materials and Methods</title>
<p id="P2">168 participants were enrolled in a 21-day experimental gingivitis investigation and grouped according to clinical measures of periodontal status of health and diseased individuals representing each of five biofilm gingival interface (BGI) periodontal groups (H, G, P1, P2, P3). Stents were used to prevent plaque removal during brushing over one maxillary and one mandibular posterior dental sextant for 21 days. Clinical periodontal parameters and unstimulated saliva were collected at screening, baseline, and each week during SIBO. Saliva samples were assessed for levels of 13 different biomarkers by multiplex immunoassay.</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Higher salivary levels of interleukin (IL)-1β, matrix metalloproteinase (MMP)-3, MMP-8, MMP-9, and neutrophil gelatinase-associated lipocalin (NGAL) were found in diseased groups compared to healthy at baseline. Conversely, higher IL-1 receptor antagonist (ra) levels were found in healthy patients at baseline. In addition, during SIBO MMP-1, tissue inhibitor of metalloproteinase (TIMP)-1, and TIMP-2 levels increased across all participant groups. A stepwise linear regression model using all salivary biomarkers demonstrated that at baseline increased IL-1ra (p=0.0044) and IL-6 (p=0.0093) were the two best predictors of change in probing depths during SIBO.</p>
</sec>
<sec id="S4"><title>Conclusions</title>
<p id="P4">In summary, this investigation supports salivary levels of IL-1ra and IL-6 as potential indicators for significant probing depth changes during induced gingival inflammation. In addition, participants from BGI-P3 group (severe periodontitis) demonstrated elevated baseline levels of IL-1β, MMP-3, MMP-8, MMP-9, and NGAL compared to other study groups strengthening the relevance of participant's biological phenotype on salivary biomarkers expression</p>
</sec>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">8000345</journal-id>
<journal-id journal-id-type="pubmed-jr-id">5144</journal-id>
<journal-id journal-id-type="nlm-ta">J Periodontol</journal-id>
<journal-id journal-id-type="iso-abbrev">J. Periodontol.</journal-id>
<journal-title-group><journal-title>Journal of periodontology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0022-3492</issn>
<issn pub-type="epub">1943-3670</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">25079398</article-id>
<article-id pub-id-type="pmc">4383599</article-id>
<article-id pub-id-type="doi">10.1902/jop.2014.140180</article-id>
<article-id pub-id-type="manuscript">NIHMS673304</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Salivary Biomarkers in a Biofilm Overgrowth Model</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Morelli</surname>
<given-names>Thiago</given-names>
</name>
<degrees>DDS, MS</degrees>
<xref ref-type="aff" rid="A1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Stella</surname>
<given-names>Michael</given-names>
</name>
<degrees>DDS, MS</degrees>
<xref ref-type="aff" rid="A1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Barros</surname>
<given-names>Silvana P.</given-names>
</name>
<degrees>DDS, MS, Ph.D</degrees>
<xref ref-type="aff" rid="A1">*</xref>
<xref ref-type="aff" rid="A2">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Marchesan</surname>
<given-names>Julie T.</given-names>
</name>
<degrees>DDS, Ph.D</degrees>
<xref ref-type="aff" rid="A2">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Moss</surname>
<given-names>Kevin L.</given-names>
</name>
<xref ref-type="aff" rid="A2">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kim</surname>
<given-names>Steven J.</given-names>
</name>
<degrees>DMD, MS</degrees>
<xref ref-type="aff" rid="A2">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yu</surname>
<given-names>Ning</given-names>
</name>
<degrees>DDS, MS</degrees>
<xref ref-type="aff" rid="A2">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Aspiras</surname>
<given-names>Marcelo B.</given-names>
</name>
<degrees>Ph.D</degrees>
<xref ref-type="aff" rid="A3">‡</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ward</surname>
<given-names>Marilyn</given-names>
</name>
<degrees>DDS</degrees>
<xref ref-type="aff" rid="A3">‡</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Offenbacher</surname>
<given-names>Steven</given-names>
</name>
<degrees>DDS, MMSc, Ph.D</degrees>
<xref ref-type="aff" rid="A1">*</xref>
<xref ref-type="aff" rid="A2">†</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>*</label>
Department of Periodontology, School of Dentistry, University of North Carolina at Chapel hill, Chapel Hill, NC, USA</aff>
<aff id="A2"><label>†</label>
Center for Oral and Systemic Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA</aff>
<aff id="A3"><label>‡</label>
Philips Oral Healthcare - Dental & Scientific Affairs, Bothell, WA, USA</aff>
<author-notes><corresp id="FN1">Corresponding author: Steven Offenbacher, Department of Periodontology, 3501F Koury Oral Health Sciences Building, Campus Box # 7455, Chapel Hill, NC27599-7455, Phone: (919)537-3205, <email>steven_offenbacher@unc.edu</email>
</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>23</day>
<month>3</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="ppub"><month>12</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>01</day>
<month>12</month>
<year>2015</year>
</pub-date>
<volume>85</volume>
<issue>12</issue>
<fpage>1770</fpage>
<lpage>1778</lpage>
<pmc-comment>elocation-id from pubmed: 10.1902/jop.2014.140180</pmc-comment>
<abstract><sec id="S1"><title>Background</title>
<p id="P1">The purpose of this study was to determine whether baseline salivary inflammatory biomarkers could discriminate between different clinical levels of disease and/or predict clinical progression over a 3-week stent-induced biofilm overgrowth (SIBO) period.</p>
</sec>
<sec id="S2"><title>Materials and Methods</title>
<p id="P2">168 participants were enrolled in a 21-day experimental gingivitis investigation and grouped according to clinical measures of periodontal status of health and diseased individuals representing each of five biofilm gingival interface (BGI) periodontal groups (H, G, P1, P2, P3). Stents were used to prevent plaque removal during brushing over one maxillary and one mandibular posterior dental sextant for 21 days. Clinical periodontal parameters and unstimulated saliva were collected at screening, baseline, and each week during SIBO. Saliva samples were assessed for levels of 13 different biomarkers by multiplex immunoassay.</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Higher salivary levels of interleukin (IL)-1β, matrix metalloproteinase (MMP)-3, MMP-8, MMP-9, and neutrophil gelatinase-associated lipocalin (NGAL) were found in diseased groups compared to healthy at baseline. Conversely, higher IL-1 receptor antagonist (ra) levels were found in healthy patients at baseline. In addition, during SIBO MMP-1, tissue inhibitor of metalloproteinase (TIMP)-1, and TIMP-2 levels increased across all participant groups. A stepwise linear regression model using all salivary biomarkers demonstrated that at baseline increased IL-1ra (p=0.0044) and IL-6 (p=0.0093) were the two best predictors of change in probing depths during SIBO.</p>
</sec>
<sec id="S4"><title>Conclusions</title>
<p id="P4">In summary, this investigation supports salivary levels of IL-1ra and IL-6 as potential indicators for significant probing depth changes during induced gingival inflammation. In addition, participants from BGI-P3 group (severe periodontitis) demonstrated elevated baseline levels of IL-1β, MMP-3, MMP-8, MMP-9, and NGAL compared to other study groups strengthening the relevance of participant's biological phenotype on salivary biomarkers expression</p>
</sec>
</abstract>
</article-meta>
</front>
</pmc>
</record>
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