Improving oral implant osseointegration in a murine model via Wnt signal amplification
Identifieur interne : 002619 ( Pmc/Corpus ); précédent : 002618; suivant : 002620Improving oral implant osseointegration in a murine model via Wnt signal amplification
Auteurs : Sylvain Mouraret ; Daniel J. Hunter ; Claire Bardet ; Antoine Popelut ; John B. Brunski ; Catherine Chaussain ; Philippe Bouchard ; Jill A. HelmsSource :
- Journal of clinical periodontology [ 0303-6979 ] ; 2013.
Abstract
To determine the key biological events occurring during implant failure and then we use this knowledge to develop new biology-based strategies that improve osseointegration.
Wild-type and
Maxillary implants lacking primary stability show histological evidence of persistent fibrous encapsulation and mobility, which recapitulates the clinical problems of implant failure. Despite histological and molecular evidence of fibrous encapsulation, osteoblasts in the gap interface exhibit robust ALP activity. This mineralization activity is counteracted by osteoclast activity that resorbs any new bony matrix and consequently, the fibrous encapsulation remains. Using a genetic mouse model, we show that implants lacking primary stability undergo osseointegration, provided that Wnt signalling is amplified.
In a mouse model of oral implant failure caused by a lack of primary stability, we find evidence of active mineralization. This mineralization, however, is outpaced by robust bone resorption, which culminates in persistent fibrous encapsulation of the implant. Fibrous encapsulation can be prevented and osseointegration assured if Wnt signalling is elevated at the time of implant placement.
Url:
DOI: 10.1111/jcpe.12187
PubMed: 24164629
PubMed Central: 4993043
Links to Exploration step
PMC:4993043Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Improving oral implant osseointegration in a murine model via Wnt signal amplification</title><author><name sortKey="Mouraret, Sylvain" sort="Mouraret, Sylvain" uniqKey="Mouraret S" first="Sylvain" last="Mouraret">Sylvain Mouraret</name><affiliation><nlm:aff id="A1">Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A2">Department of Periodontology, Service of Odontology, Rothschild Hospital, AP-HP, Paris 7 – Denis, Diderot University, U.F.R. of Odontology, Paris, France</nlm:aff></affiliation></author><author><name sortKey="Hunter, Daniel J" sort="Hunter, Daniel J" uniqKey="Hunter D" first="Daniel J." last="Hunter">Daniel J. Hunter</name><affiliation><nlm:aff id="A1">Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA, USA</nlm:aff></affiliation></author><author><name sortKey="Bardet, Claire" sort="Bardet, Claire" uniqKey="Bardet C" first="Claire" last="Bardet">Claire Bardet</name><affiliation><nlm:aff id="A1">Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A3">Dental School University Paris Descartes PRES Sorbonne Paris Cité, Montrouge, France</nlm:aff></affiliation></author><author><name sortKey="Popelut, Antoine" sort="Popelut, Antoine" uniqKey="Popelut A" first="Antoine" last="Popelut">Antoine Popelut</name><affiliation><nlm:aff id="A2">Department of Periodontology, Service of Odontology, Rothschild Hospital, AP-HP, Paris 7 – Denis, Diderot University, U.F.R. of Odontology, Paris, France</nlm:aff></affiliation></author><author><name sortKey="Brunski, John B" sort="Brunski, John B" uniqKey="Brunski J" first="John B." last="Brunski">John B. Brunski</name><affiliation><nlm:aff id="A1">Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA, USA</nlm:aff></affiliation></author><author><name sortKey="Chaussain, Catherine" sort="Chaussain, Catherine" uniqKey="Chaussain C" first="Catherine" last="Chaussain">Catherine Chaussain</name><affiliation><nlm:aff id="A3">Dental School University Paris Descartes PRES Sorbonne Paris Cité, Montrouge, France</nlm:aff></affiliation></author><author><name sortKey="Bouchard, Philippe" sort="Bouchard, Philippe" uniqKey="Bouchard P" first="Philippe" last="Bouchard">Philippe Bouchard</name><affiliation><nlm:aff id="A2">Department of Periodontology, Service of Odontology, Rothschild Hospital, AP-HP, Paris 7 – Denis, Diderot University, U.F.R. of Odontology, Paris, France</nlm:aff></affiliation></author><author><name sortKey="Helms, Jill A" sort="Helms, Jill A" uniqKey="Helms J" first="Jill A." last="Helms">Jill A. Helms</name><affiliation><nlm:aff id="A1">Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA, USA</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24164629</idno><idno type="pmc">4993043</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4993043</idno><idno type="RBID">PMC:4993043</idno><idno type="doi">10.1111/jcpe.12187</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">002619</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002619</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Improving oral implant osseointegration in a murine model via Wnt signal amplification</title><author><name sortKey="Mouraret, Sylvain" sort="Mouraret, Sylvain" uniqKey="Mouraret S" first="Sylvain" last="Mouraret">Sylvain Mouraret</name><affiliation><nlm:aff id="A1">Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A2">Department of Periodontology, Service of Odontology, Rothschild Hospital, AP-HP, Paris 7 – Denis, Diderot University, U.F.R. of Odontology, Paris, France</nlm:aff></affiliation></author><author><name sortKey="Hunter, Daniel J" sort="Hunter, Daniel J" uniqKey="Hunter D" first="Daniel J." last="Hunter">Daniel J. Hunter</name><affiliation><nlm:aff id="A1">Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA, USA</nlm:aff></affiliation></author><author><name sortKey="Bardet, Claire" sort="Bardet, Claire" uniqKey="Bardet C" first="Claire" last="Bardet">Claire Bardet</name><affiliation><nlm:aff id="A1">Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA, USA</nlm:aff></affiliation><affiliation><nlm:aff id="A3">Dental School University Paris Descartes PRES Sorbonne Paris Cité, Montrouge, France</nlm:aff></affiliation></author><author><name sortKey="Popelut, Antoine" sort="Popelut, Antoine" uniqKey="Popelut A" first="Antoine" last="Popelut">Antoine Popelut</name><affiliation><nlm:aff id="A2">Department of Periodontology, Service of Odontology, Rothschild Hospital, AP-HP, Paris 7 – Denis, Diderot University, U.F.R. of Odontology, Paris, France</nlm:aff></affiliation></author><author><name sortKey="Brunski, John B" sort="Brunski, John B" uniqKey="Brunski J" first="John B." last="Brunski">John B. Brunski</name><affiliation><nlm:aff id="A1">Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA, USA</nlm:aff></affiliation></author><author><name sortKey="Chaussain, Catherine" sort="Chaussain, Catherine" uniqKey="Chaussain C" first="Catherine" last="Chaussain">Catherine Chaussain</name><affiliation><nlm:aff id="A3">Dental School University Paris Descartes PRES Sorbonne Paris Cité, Montrouge, France</nlm:aff></affiliation></author><author><name sortKey="Bouchard, Philippe" sort="Bouchard, Philippe" uniqKey="Bouchard P" first="Philippe" last="Bouchard">Philippe Bouchard</name><affiliation><nlm:aff id="A2">Department of Periodontology, Service of Odontology, Rothschild Hospital, AP-HP, Paris 7 – Denis, Diderot University, U.F.R. of Odontology, Paris, France</nlm:aff></affiliation></author><author><name sortKey="Helms, Jill A" sort="Helms, Jill A" uniqKey="Helms J" first="Jill A." last="Helms">Jill A. Helms</name><affiliation><nlm:aff id="A1">Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA, USA</nlm:aff></affiliation></author></analytic><series><title level="j">Journal of clinical periodontology</title><idno type="ISSN">0303-6979</idno><idno type="eISSN">1600-051X</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="S1"><title>Aim</title><p id="P1">To determine the key biological events occurring during implant failure and then we use this knowledge to develop new biology-based strategies that improve osseointegration.</p></sec><sec id="S2"><title>Materials and Methods</title><p id="P2">Wild-type and <italic>Axin2<sup>LacZ/LacZ</sup></italic> adult male mice underwent oral implant placement, with and without primary stability. Peri-implant tissues were evaluated using histology, alkaline phosphatase (ALP) activity, tartrate resistant acid phosphatase (TRAP) activity and TUNEL staining. In addition, mineralization sites, collagenous matrix organization and the expression of bone markers in the peri-implant tissues were assessed.</p></sec><sec id="S3"><title>Results</title><p id="P3">Maxillary implants lacking primary stability show histological evidence of persistent fibrous encapsulation and mobility, which recapitulates the clinical problems of implant failure. Despite histological and molecular evidence of fibrous encapsulation, osteoblasts in the gap interface exhibit robust ALP activity. This mineralization activity is counteracted by osteoclast activity that resorbs any new bony matrix and consequently, the fibrous encapsulation remains. Using a genetic mouse model, we show that implants lacking primary stability undergo osseointegration, provided that Wnt signalling is amplified.</p></sec><sec id="S4"><title>Conclusions</title><p id="P4">In a mouse model of oral implant failure caused by a lack of primary stability, we find evidence of active mineralization. This mineralization, however, is outpaced by robust bone resorption, which culminates in persistent fibrous encapsulation of the implant. Fibrous encapsulation can be prevented and osseointegration assured if Wnt signalling is elevated at the time of implant placement.</p></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">0425123</journal-id><journal-id journal-id-type="pubmed-jr-id">4645</journal-id><journal-id journal-id-type="nlm-ta">J Clin Periodontol</journal-id><journal-id journal-id-type="iso-abbrev">J. Clin. Periodontol.</journal-id><journal-title-group><journal-title>Journal of clinical periodontology</journal-title></journal-title-group><issn pub-type="ppub">0303-6979</issn><issn pub-type="epub">1600-051X</issn></journal-meta><article-meta><article-id pub-id-type="pmid">24164629</article-id><article-id pub-id-type="pmc">4993043</article-id><article-id pub-id-type="doi">10.1111/jcpe.12187</article-id><article-id pub-id-type="manuscript">NIHMS809778</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Improving oral implant osseointegration in a murine model via Wnt signal amplification</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Mouraret</surname><given-names>Sylvain</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Hunter</surname><given-names>Daniel J.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Bardet</surname><given-names>Claire</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref ref-type="aff" rid="A3">3</xref></contrib><contrib contrib-type="author"><name><surname>Popelut</surname><given-names>Antoine</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Brunski</surname><given-names>John B.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Chaussain</surname><given-names>Catherine</given-names></name><xref ref-type="aff" rid="A3">3</xref></contrib><contrib contrib-type="author"><name><surname>Bouchard</surname><given-names>Philippe</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Helms</surname><given-names>Jill A.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib></contrib-group><aff id="A1"><label>1</label>Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA, USA</aff><aff id="A2"><label>2</label>Department of Periodontology, Service of Odontology, Rothschild Hospital, AP-HP, Paris 7 – Denis, Diderot University, U.F.R. of Odontology, Paris, France</aff><aff id="A3"><label>3</label>Dental School University Paris Descartes PRES Sorbonne Paris Cité, Montrouge, France</aff><author-notes><corresp id="FN1">Address: Jill A. Helms, 257 Campus Drive, PSRL Building, Stanford, CA 94305, USA, <email>jhelms@stanford.edu</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>16</day><month>8</month><year>2016</year></pub-date><pub-date pub-type="epub"><day>08</day><month>12</month><year>2013</year></pub-date><pub-date pub-type="ppub"><month>2</month><year>2014</year></pub-date><pub-date pub-type="pmc-release"><day>22</day><month>8</month><year>2016</year></pub-date><volume>41</volume><issue>2</issue><fpage>172</fpage><lpage>180</lpage><pmc-comment>elocation-id from pubmed: 10.1111/jcpe.12187</pmc-comment>
<abstract><sec id="S1"><title>Aim</title><p id="P1">To determine the key biological events occurring during implant failure and then we use this knowledge to develop new biology-based strategies that improve osseointegration.</p></sec><sec id="S2"><title>Materials and Methods</title><p id="P2">Wild-type and <italic>Axin2<sup>LacZ/LacZ</sup></italic> adult male mice underwent oral implant placement, with and without primary stability. Peri-implant tissues were evaluated using histology, alkaline phosphatase (ALP) activity, tartrate resistant acid phosphatase (TRAP) activity and TUNEL staining. In addition, mineralization sites, collagenous matrix organization and the expression of bone markers in the peri-implant tissues were assessed.</p></sec><sec id="S3"><title>Results</title><p id="P3">Maxillary implants lacking primary stability show histological evidence of persistent fibrous encapsulation and mobility, which recapitulates the clinical problems of implant failure. Despite histological and molecular evidence of fibrous encapsulation, osteoblasts in the gap interface exhibit robust ALP activity. This mineralization activity is counteracted by osteoclast activity that resorbs any new bony matrix and consequently, the fibrous encapsulation remains. Using a genetic mouse model, we show that implants lacking primary stability undergo osseointegration, provided that Wnt signalling is amplified.</p></sec><sec id="S4"><title>Conclusions</title><p id="P4">In a mouse model of oral implant failure caused by a lack of primary stability, we find evidence of active mineralization. This mineralization, however, is outpaced by robust bone resorption, which culminates in persistent fibrous encapsulation of the implant. Fibrous encapsulation can be prevented and osseointegration assured if Wnt signalling is elevated at the time of implant placement.</p></sec></abstract><kwd-group><kwd>Axin2</kwd><kwd>bone</kwd><kwd>dental</kwd><kwd>fibrointegration</kwd><kwd>fibrous encapsulation</kwd><kwd>histology</kwd><kwd><italic>in vivo</italic></kwd><kwd>maxilla</kwd><kwd>mice</kwd><kwd>model</kwd><kwd>oral cavity</kwd><kwd>Wnt</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/EdenteV2/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002619 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 002619 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Santé
|area= EdenteV2
|flux= Pmc
|étape= Corpus
|type= RBID
|clé= PMC:4993043
|texte= Improving oral implant osseointegration in a murine model via Wnt signal amplification
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:24164629" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a EdenteV2
| This area was generated with Dilib version V0.6.32. |  |