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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Implant-abutment leaking of replace conical connection nobel biocare<sup>®</sup>
implant system. An <italic>in vitro</italic>
study of the microbiological penetration from external environment to implant-abutment space</title>
<author><name sortKey="El Haddad, E" sort="El Haddad, E" uniqKey="El Haddad E" first="E." last="El Haddad">E. El Haddad</name>
<affiliation><nlm:aff id="af1-76-82">Private practice, Turin, Italy</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gianni, A B" sort="Gianni, A B" uniqKey="Gianni A" first="A. B." last="Giannì">A. B. Giannì</name>
<affiliation><nlm:aff id="af2-76-82">Maxillo-Facial and Dental Unit, “Fondazione Cà Granda IRCCS Ospedale Maggiore Policlinico”, Milano, Italy</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mancini, G E" sort="Mancini, G E" uniqKey="Mancini G" first="G. E." last="Mancini">G. E. Mancini</name>
<affiliation><nlm:aff id="af2-76-82">Maxillo-Facial and Dental Unit, “Fondazione Cà Granda IRCCS Ospedale Maggiore Policlinico”, Milano, Italy</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Cura, F" sort="Cura, F" uniqKey="Cura F" first="F." last="Cura">F. Cura</name>
<affiliation><nlm:aff id="af3-76-82">Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Carinci, F" sort="Carinci, F" uniqKey="Carinci F" first="F." last="Carinci">F. Carinci</name>
<affiliation><nlm:aff id="af4-76-82">Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">28042434</idno>
<idno type="pmc">5159912</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159912</idno>
<idno type="RBID">PMC:5159912</idno>
<idno type="doi">10.11138/orl/2016.9.2.076</idno>
<date when="2016">2016</date>
<idno type="wicri:Area/Pmc/Corpus">002520</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002520</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Implant-abutment leaking of replace conical connection nobel biocare<sup>®</sup>
implant system. An <italic>in vitro</italic>
study of the microbiological penetration from external environment to implant-abutment space</title>
<author><name sortKey="El Haddad, E" sort="El Haddad, E" uniqKey="El Haddad E" first="E." last="El Haddad">E. El Haddad</name>
<affiliation><nlm:aff id="af1-76-82">Private practice, Turin, Italy</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gianni, A B" sort="Gianni, A B" uniqKey="Gianni A" first="A. B." last="Giannì">A. B. Giannì</name>
<affiliation><nlm:aff id="af2-76-82">Maxillo-Facial and Dental Unit, “Fondazione Cà Granda IRCCS Ospedale Maggiore Policlinico”, Milano, Italy</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mancini, G E" sort="Mancini, G E" uniqKey="Mancini G" first="G. E." last="Mancini">G. E. Mancini</name>
<affiliation><nlm:aff id="af2-76-82">Maxillo-Facial and Dental Unit, “Fondazione Cà Granda IRCCS Ospedale Maggiore Policlinico”, Milano, Italy</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Cura, F" sort="Cura, F" uniqKey="Cura F" first="F." last="Cura">F. Cura</name>
<affiliation><nlm:aff id="af3-76-82">Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Carinci, F" sort="Carinci, F" uniqKey="Carinci F" first="F." last="Carinci">F. Carinci</name>
<affiliation><nlm:aff id="af4-76-82">Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Oral & Implantology</title>
<idno type="ISSN">1974-5648</idno>
<idno type="eISSN">2035-2468</idno>
<imprint><date when="2016">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>SUMMARY</title>
<sec><title>Purpose</title>
<p>The aim of our study is to value the microbial contamination in the implant-abutment connections (IAC) of a Nobel Replace Conical Connection implant system [Nobel Biocare®, Vimercate (MB), Italy].</p>
</sec>
<sec><title>Materials and methods</title>
<p>To identify the capability of the implant to protect the internal space from the external environment, the passage of genetically modified bacteria across IAC was evaluated. Four Nobel Replace Conical Connection implants (Nobel Biocare®, Vimercate (MB), Italy) were immerged in a bacterial culture for twenty-four hours and then bacteria amount was measured inside and outside IAC with Real-time PCR. Bacterial quantification was performed by Real-Time Polymerase Chain Reaction using the absolute quantification with the standard curve method.</p>
</sec>
<sec><title>Results</title>
<p>In all tested implants, bacteria were found in the inner side, with a median percentage of 10.9%. The analysis revealed that in both cases (internally and externally), bacteria grew for the first 48 hours but subsequently they started to dye, probably as a consequence of nutrient consumption. Moreover, the difference between outer and inner bacteria concentration was statistically significant at each time point.</p>
</sec>
<sec><title>Conclusions</title>
<p>Implant’s internal contamination shows that IAC is not sealing. The reported results are similar to those of previous studies carried out on different implant systems. Until now, no IAC has been proven to seal the gap between implant and abutment.</p>
</sec>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Oral Implantol (Rome)</journal-id>
<journal-id journal-id-type="iso-abbrev">Oral Implantol (Rome)</journal-id>
<journal-id journal-id-type="publisher-id">OAI</journal-id>
<journal-title-group><journal-title>Oral & Implantology</journal-title>
</journal-title-group>
<issn pub-type="ppub">1974-5648</issn>
<issn pub-type="epub">2035-2468</issn>
<publisher><publisher-name>CIC Edizioni Internazionali</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">28042434</article-id>
<article-id pub-id-type="pmc">5159912</article-id>
<article-id pub-id-type="doi">10.11138/orl/2016.9.2.076</article-id>
<article-id pub-id-type="publisher-id">76-82</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Implant-abutment leaking of replace conical connection nobel biocare<sup>®</sup>
implant system. An <italic>in vitro</italic>
study of the microbiological penetration from external environment to implant-abutment space</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>EL HADDAD</surname>
<given-names>E.</given-names>
</name>
<xref ref-type="aff" rid="af1-76-82">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>GIANNÌ</surname>
<given-names>A.B.</given-names>
</name>
<xref ref-type="aff" rid="af2-76-82">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>MANCINI</surname>
<given-names>G.E.</given-names>
</name>
<xref ref-type="aff" rid="af2-76-82">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>CURA</surname>
<given-names>F.</given-names>
</name>
<xref ref-type="aff" rid="af3-76-82">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>CARINCI</surname>
<given-names>F.</given-names>
</name>
<xref ref-type="aff" rid="af4-76-82">4</xref>
<xref ref-type="corresp" rid="c1-76-82"></xref>
</contrib>
</contrib-group>
<aff id="af1-76-82"><label>1</label>
Private practice, Turin, Italy</aff>
<aff id="af2-76-82"><label>2</label>
Maxillo-Facial and Dental Unit, “Fondazione Cà Granda IRCCS Ospedale Maggiore Policlinico”, Milano, Italy</aff>
<aff id="af3-76-82"><label>3</label>
Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy</aff>
<aff id="af4-76-82"><label>4</label>
Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy</aff>
<author-notes><corresp id="c1-76-82">Correspondence to: Prof. Francesco Carinci, M.D., Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Via Luigi Borsari 46, 44121 Ferrara, Italy, Phone: +39 0532 455874 - Fax: +39 0532 455876, E-mail: <email>crc@unife.it</email>
</corresp>
</author-notes>
<pub-date pub-type="collection"><season>Apr-Jun</season>
<year>2016</year>
</pub-date>
<pub-date pub-type="epub"><day>13</day>
<month>11</month>
<year>2016</year>
</pub-date>
<volume>9</volume>
<issue>2</issue>
<fpage>76</fpage>
<lpage>82</lpage>
<permissions><copyright-statement>© Copyright 2016, CIC Edizioni Internazionali, Roma</copyright-statement>
<copyright-year>2016</copyright-year>
</permissions>
<abstract><title>SUMMARY</title>
<sec><title>Purpose</title>
<p>The aim of our study is to value the microbial contamination in the implant-abutment connections (IAC) of a Nobel Replace Conical Connection implant system [Nobel Biocare®, Vimercate (MB), Italy].</p>
</sec>
<sec><title>Materials and methods</title>
<p>To identify the capability of the implant to protect the internal space from the external environment, the passage of genetically modified bacteria across IAC was evaluated. Four Nobel Replace Conical Connection implants (Nobel Biocare®, Vimercate (MB), Italy) were immerged in a bacterial culture for twenty-four hours and then bacteria amount was measured inside and outside IAC with Real-time PCR. Bacterial quantification was performed by Real-Time Polymerase Chain Reaction using the absolute quantification with the standard curve method.</p>
</sec>
<sec><title>Results</title>
<p>In all tested implants, bacteria were found in the inner side, with a median percentage of 10.9%. The analysis revealed that in both cases (internally and externally), bacteria grew for the first 48 hours but subsequently they started to dye, probably as a consequence of nutrient consumption. Moreover, the difference between outer and inner bacteria concentration was statistically significant at each time point.</p>
</sec>
<sec><title>Conclusions</title>
<p>Implant’s internal contamination shows that IAC is not sealing. The reported results are similar to those of previous studies carried out on different implant systems. Until now, no IAC has been proven to seal the gap between implant and abutment.</p>
</sec>
</abstract>
<kwd-group><kwd>bone resorption</kwd>
<kwd>implant-abutment connection</kwd>
<kwd>microbiological leakage</kwd>
<kwd>and perimplantitis</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
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