Use of CHROMagar Candida medium for isolation of yeasts from dental samples.
Identifieur interne : 001575 ( Pmc/Corpus ); précédent : 001574; suivant : 001576Use of CHROMagar Candida medium for isolation of yeasts from dental samples.
Auteurs : D. Beighton ; R. Ludford ; D T Clark ; S R Brailsford ; C L Pankhurst ; G F Tinsley ; J. Fiske ; D. Lewis ; B. Daly ; N. KhalifaSource :
- Journal of Clinical Microbiology [ 0095-1137 ] ; 1995.
Abstract
A new differential medium, CHROMagar Candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. The recovery of yeasts on the medium was not significantly different from the recovery on Sabouraud dextrose agar. The identities of 450 green colonies on CHROMagar Candida, presumptively identified as Candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-N-acetylgalactosaminidase. Candida tropicalis also formed distinctive colonies, and other yeasts including Candida (Torulopsis) glabrata, Candida Parapsilosis, Candida Magnoliae, Candida lusitaniae, Candida Famata, Candida kefir, and Saccharomyces cerevisiae were readily distinguished from C. albicans and C. tropicalis isolates. CHROMagar Candida is a very useful medium, and its use will facilitate the study of yeasts associated with dental diseases.
Url:
PubMed: 8576366
PubMed Central: 228627
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PMC:228627Le document en format XML
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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id><journal-title>Journal of Clinical Microbiology</journal-title><issn pub-type="ppub">0095-1137</issn><issn pub-type="epub">1098-660X</issn></journal-meta><article-meta><article-id pub-id-type="pmid">8576366</article-id><article-id pub-id-type="pmc">228627</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Use of CHROMagar Candida medium for isolation of yeasts from dental samples.</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Beighton</surname><given-names>D</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ludford</surname><given-names>R</given-names></name></contrib><contrib contrib-type="author"><name><surname>Clark</surname><given-names>D T</given-names></name></contrib><contrib contrib-type="author"><name><surname>Brailsford</surname><given-names>S R</given-names></name></contrib><contrib contrib-type="author"><name><surname>Pankhurst</surname><given-names>C L</given-names></name></contrib><contrib contrib-type="author"><name><surname>Tinsley</surname><given-names>G F</given-names></name></contrib><contrib contrib-type="author"><name><surname>Fiske</surname><given-names>J</given-names></name></contrib><contrib contrib-type="author"><name><surname>Lewis</surname><given-names>D</given-names></name></contrib><contrib contrib-type="author"><name><surname>Daly</surname><given-names>B</given-names></name></contrib><contrib contrib-type="author"><name><surname>Khalifa</surname><given-names>N</given-names></name></contrib></contrib-group><aff id="N0x8b2dcd0.0x97cc598">RCS Department of Dental Sciences, King's College School of Medicine and Dentistry, London, United Kingdom.</aff><pub-date pub-type="ppub"><month>11</month><year>1995</year></pub-date><volume>33</volume><issue>11</issue><fpage>3025</fpage><lpage>3027</lpage><abstract><p>A new differential medium, CHROMagar Candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. The recovery of yeasts on the medium was not significantly different from the recovery on Sabouraud dextrose agar. The identities of 450 green colonies on CHROMagar Candida, presumptively identified as Candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-N-acetylgalactosaminidase. Candida tropicalis also formed distinctive colonies, and other yeasts including Candida (Torulopsis) glabrata, Candida Parapsilosis, Candida Magnoliae, Candida lusitaniae, Candida Famata, Candida kefir, and Saccharomyces cerevisiae were readily distinguished from C. albicans and C. tropicalis isolates. CHROMagar Candida is a very useful medium, and its use will facilitate the study of yeasts associated with dental diseases.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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