Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction.
Identifieur interne : 001573 ( Pmc/Corpus ); précédent : 001572; suivant : 001574Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction.
Auteurs : A M Nguyen ; L. Engstrand ; R M Genta ; D Y Graham ; F A El-ZaatariSource :
- Journal of Clinical Microbiology [ 0095-1137 ] ; 1993.
Abstract
To investigate whether the oral cavity is a potential reservoir and possible sanctuary for Helicobacter pylori, supragingival and subgingival plaques were analyzed by a Helicobacter genus-specific reverse transcriptase-polymerase chain reaction based on the sequence data of H. pylori 16S rRNA. The amplified 500-bp DNA fragment was identified by ethidium bromide staining after agarose gel electrophoresis and by Southern hybridization. Twenty-five dyspeptic patients were studied. Histologic examination of gastric biopsy specimens revealed that 18 had H. pylori gastritis and 7 did not. For seven of the 18 (38.8%) patients with proven H. pylori gastritis, H. pylori was also identified in their dental plaque. None of the patients without H. pylori gastritis had H. pylori in their dental plaque. The detection of H. pylori in dental plaque suggests that this H. pylori colonization is not restricted to the gastric mucosa and that this ecological niche may serve as a possible sanctuary which may be responsible for reinoculation of the stomach after topical anti-H. pylori therapies such as bismuth.
Url:
PubMed: 8463387
PubMed Central: 263562
Links to Exploration step
PMC:263562Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction.</title>
<author><name sortKey="Nguyen, A M" sort="Nguyen, A M" uniqKey="Nguyen A" first="A M" last="Nguyen">A M Nguyen</name>
</author>
<author><name sortKey="Engstrand, L" sort="Engstrand, L" uniqKey="Engstrand L" first="L" last="Engstrand">L. Engstrand</name>
</author>
<author><name sortKey="Genta, R M" sort="Genta, R M" uniqKey="Genta R" first="R M" last="Genta">R M Genta</name>
</author>
<author><name sortKey="Graham, D Y" sort="Graham, D Y" uniqKey="Graham D" first="D Y" last="Graham">D Y Graham</name>
</author>
<author><name sortKey="El Zaatari, F A" sort="El Zaatari, F A" uniqKey="El Zaatari F" first="F A" last="El-Zaatari">F A El-Zaatari</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">8463387</idno>
<idno type="pmc">263562</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC263562</idno>
<idno type="RBID">PMC:263562</idno>
<date when="1993">1993</date>
<idno type="wicri:Area/Pmc/Corpus">001573</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001573</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction.</title>
<author><name sortKey="Nguyen, A M" sort="Nguyen, A M" uniqKey="Nguyen A" first="A M" last="Nguyen">A M Nguyen</name>
</author>
<author><name sortKey="Engstrand, L" sort="Engstrand, L" uniqKey="Engstrand L" first="L" last="Engstrand">L. Engstrand</name>
</author>
<author><name sortKey="Genta, R M" sort="Genta, R M" uniqKey="Genta R" first="R M" last="Genta">R M Genta</name>
</author>
<author><name sortKey="Graham, D Y" sort="Graham, D Y" uniqKey="Graham D" first="D Y" last="Graham">D Y Graham</name>
</author>
<author><name sortKey="El Zaatari, F A" sort="El Zaatari, F A" uniqKey="El Zaatari F" first="F A" last="El-Zaatari">F A El-Zaatari</name>
</author>
</analytic>
<series><title level="j">Journal of Clinical Microbiology</title>
<idno type="ISSN">0095-1137</idno>
<idno type="eISSN">1098-660X</idno>
<imprint><date when="1993">1993</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>To investigate whether the oral cavity is a potential reservoir and possible sanctuary for Helicobacter pylori, supragingival and subgingival plaques were analyzed by a Helicobacter genus-specific reverse transcriptase-polymerase chain reaction based on the sequence data of H. pylori 16S rRNA. The amplified 500-bp DNA fragment was identified by ethidium bromide staining after agarose gel electrophoresis and by Southern hybridization. Twenty-five dyspeptic patients were studied. Histologic examination of gastric biopsy specimens revealed that 18 had H. pylori gastritis and 7 did not. For seven of the 18 (38.8%) patients with proven H. pylori gastritis, H. pylori was also identified in their dental plaque. None of the patients without H. pylori gastritis had H. pylori in their dental plaque. The detection of H. pylori in dental plaque suggests that this H. pylori colonization is not restricted to the gastric mucosa and that this ecological niche may serve as a possible sanctuary which may be responsible for reinoculation of the stomach after topical anti-H. pylori therapies such as bismuth.</p>
<sec sec-type="scanned-figures"><title>Images</title>
<fig id="F1"><graphic xlink:href="jcm00016-0033-a" xlink:role="785"></graphic>
</fig>
</sec>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id>
<journal-title>Journal of Clinical Microbiology</journal-title>
<issn pub-type="ppub">0095-1137</issn>
<issn pub-type="epub">1098-660X</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">8463387</article-id>
<article-id pub-id-type="pmc">263562</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction.</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Nguyen</surname>
<given-names>A M</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Engstrand</surname>
<given-names>L</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Genta</surname>
<given-names>R M</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Graham</surname>
<given-names>D Y</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>el-Zaatari</surname>
<given-names>F A</given-names>
</name>
</contrib>
</contrib-group>
<aff>Department of Medicine, Baylor College of Medicine, Houston, Texas.</aff>
<pub-date pub-type="ppub"><month>4</month>
<year>1993</year>
</pub-date>
<volume>31</volume>
<issue>4</issue>
<fpage>783</fpage>
<lpage>787</lpage>
<abstract><p>To investigate whether the oral cavity is a potential reservoir and possible sanctuary for Helicobacter pylori, supragingival and subgingival plaques were analyzed by a Helicobacter genus-specific reverse transcriptase-polymerase chain reaction based on the sequence data of H. pylori 16S rRNA. The amplified 500-bp DNA fragment was identified by ethidium bromide staining after agarose gel electrophoresis and by Southern hybridization. Twenty-five dyspeptic patients were studied. Histologic examination of gastric biopsy specimens revealed that 18 had H. pylori gastritis and 7 did not. For seven of the 18 (38.8%) patients with proven H. pylori gastritis, H. pylori was also identified in their dental plaque. None of the patients without H. pylori gastritis had H. pylori in their dental plaque. The detection of H. pylori in dental plaque suggests that this H. pylori colonization is not restricted to the gastric mucosa and that this ecological niche may serve as a possible sanctuary which may be responsible for reinoculation of the stomach after topical anti-H. pylori therapies such as bismuth.</p>
<sec sec-type="scanned-figures"><title>Images</title>
<fig id="F1"><graphic xlink:href="jcm00016-0033-a" xlink:role="785"></graphic>
</fig>
</sec>
</abstract>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/EdenteV2/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001573 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 001573 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Santé |area= EdenteV2 |flux= Pmc |étape= Corpus |type= RBID |clé= PMC:263562 |texte= Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:8463387" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a EdenteV2
![]() | This area was generated with Dilib version V0.6.32. | ![]() |