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Leptotrichia amnionii sp. nov., a Novel Bacterium Isolated from the Amniotic Fluid of a Woman after Intrauterine Fetal Demise

Identifieur interne : 001569 ( Pmc/Corpus ); précédent : 001568; suivant : 001570

Leptotrichia amnionii sp. nov., a Novel Bacterium Isolated from the Amniotic Fluid of a Woman after Intrauterine Fetal Demise

Auteurs : Sanjay K. Shukla ; Paul R. Meier ; Paul D. Mitchell ; Daniel N. Frank ; Kurt D. Reed

Source :

RBID : PMC:130742

Abstract

A novel bacterium was isolated and characterized from the amniotic fluid of a woman who experienced intrauterine fetal demise in the second trimester of pregnancy. The bacterium was a slow-growing, gram-negative anaerobic coccobacillus belonging to the genus Leptotrichia. Unlike Leptotrichia sanguinegens, the isolate did not grow in chopped-meat glucose broth or on sheep blood agar upon subculturing. The isolate was characterized by sequencing and analyzing its 16S rRNA gene. The 1,493-bp 16S ribosomal DNA sequence had only 96% homology with L. sanguinegens. Several phylogenetic analyses indicated that L. amnionii is a distinct species and most closely related to L. sanguiegens.


Url:
DOI: 10.1128/JCM.40.9.3346-3349.2002
PubMed: 12202577
PubMed Central: 130742

Links to Exploration step

PMC:130742

Le document en format XML

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<italic>Leptotrichia amnionii</italic>
sp. nov., a Novel Bacterium Isolated from the Amniotic Fluid of a Woman after Intrauterine Fetal Demise</title>
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<name sortKey="Shukla, Sanjay K" sort="Shukla, Sanjay K" uniqKey="Shukla S" first="Sanjay K." last="Shukla">Sanjay K. Shukla</name>
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<name sortKey="Meier, Paul R" sort="Meier, Paul R" uniqKey="Meier P" first="Paul R." last="Meier">Paul R. Meier</name>
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<title level="j">Journal of Clinical Microbiology</title>
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<p>A novel bacterium was isolated and characterized from the amniotic fluid of a woman who experienced intrauterine fetal demise in the second trimester of pregnancy. The bacterium was a slow-growing, gram-negative anaerobic coccobacillus belonging to the genus
<italic>Leptotrichia</italic>
. Unlike
<italic>Leptotrichia sanguinegens</italic>
, the isolate did not grow in chopped-meat glucose broth or on sheep blood agar upon subculturing. The isolate was characterized by sequencing and analyzing its 16S rRNA gene. The 1,493-bp 16S ribosomal DNA sequence had only 96% homology with
<italic>L. sanguinegens.</italic>
Several phylogenetic analyses indicated that
<italic>L. amnionii</italic>
is a distinct species and most closely related to
<italic>L. sanguiegens</italic>
.</p>
</div>
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<journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">jcm</journal-id>
<journal-title>Journal of Clinical Microbiology</journal-title>
<issn pub-type="ppub">0095-1137</issn>
<issn pub-type="epub">1098-660X</issn>
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<article-id pub-id-type="publisher-id">0376</article-id>
<article-id pub-id-type="doi">10.1128/JCM.40.9.3346-3349.2002</article-id>
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<article-title>
<italic>Leptotrichia amnionii</italic>
sp. nov., a Novel Bacterium Isolated from the Amniotic Fluid of a Woman after Intrauterine Fetal Demise</article-title>
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<contrib-group>
<contrib contrib-type="author">
<name>
<surname>Shukla</surname>
<given-names>Sanjay K.</given-names>
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<xref ref-type="aff" rid="aff1">1</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
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<contrib contrib-type="author">
<name>
<surname>Meier</surname>
<given-names>Paul R.</given-names>
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<xref ref-type="aff" rid="aff1">2</xref>
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<surname>Mitchell</surname>
<given-names>Paul D.</given-names>
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<surname>Frank</surname>
<given-names>Daniel N.</given-names>
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<xref ref-type="aff" rid="aff1">4</xref>
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<name>
<surname>Reed</surname>
<given-names>Kurt D.</given-names>
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<aff id="aff1">Clinical Research Center, Marshfield Medical Research and Education Foundation,
<label>1</label>
Department of Obstetrics and Gynecology, Marshfield Clinic,
<label>2</label>
Marshfield Laboratories, Marshfield, Wisconsin 54449,
<label>3</label>
Department of Molecular, Cellular, and Developmental Biology, The University of Colorado, Boulder, Colorado 80309
<label>4</label>
</aff>
<author-notes>
<fn id="cor1">
<label>*</label>
<p>Corresponding author. Mailing address: Clinical Research Center, Marshfield Medical Research and Education Foundation, 1000 North Oak Avenue, Marshfield, WI 54449. Phone: (715) 389-5363. Fax: (715) 389-3808. E-mail:
<email>shuklas@mmrf.mfldclin.edu</email>
.</p>
</fn>
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<pub-date pub-type="ppub">
<month>9</month>
<year>2002</year>
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<volume>40</volume>
<issue>9</issue>
<fpage>3346</fpage>
<lpage>3349</lpage>
<history>
<date date-type="received">
<day>18</day>
<month>3</month>
<year>2002</year>
</date>
<date date-type="rev-recd">
<day>21</day>
<month>6</month>
<year>2002</year>
</date>
<date date-type="accepted">
<day>30</day>
<month>6</month>
<year>2002</year>
</date>
</history>
<copyright-statement>Copyright © 2002, American Society for Microbiology</copyright-statement>
<copyright-year>2002</copyright-year>
<abstract>
<p>A novel bacterium was isolated and characterized from the amniotic fluid of a woman who experienced intrauterine fetal demise in the second trimester of pregnancy. The bacterium was a slow-growing, gram-negative anaerobic coccobacillus belonging to the genus
<italic>Leptotrichia</italic>
. Unlike
<italic>Leptotrichia sanguinegens</italic>
, the isolate did not grow in chopped-meat glucose broth or on sheep blood agar upon subculturing. The isolate was characterized by sequencing and analyzing its 16S rRNA gene. The 1,493-bp 16S ribosomal DNA sequence had only 96% homology with
<italic>L. sanguinegens.</italic>
Several phylogenetic analyses indicated that
<italic>L. amnionii</italic>
is a distinct species and most closely related to
<italic>L. sanguiegens</italic>
.</p>
</abstract>
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