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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Novel Factor Isolated from <italic>Actinobacillus actinomycetemcomitans</italic> Stimulates Mouse B Cells and Human Peripheral Blood Mononuclear Cells</title><author><name sortKey="Jeong, Soo Jin" sort="Jeong, Soo Jin" uniqKey="Jeong S" first="Soo-Jin" last="Jeong">Soo-Jin Jeong</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Yee, Sung Tae" sort="Yee, Sung Tae" uniqKey="Yee S" first="Sung-Tae" last="Yee">Sung-Tae Yee</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Jo, Wol Soon" sort="Jo, Wol Soon" uniqKey="Jo W" first="Wol-Soon" last="Jo">Wol-Soon Jo</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Yu, Seong Ho" sort="Yu, Seong Ho" uniqKey="Yu S" first="Seong-Ho" last="Yu">Seong-Ho Yu</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Lee, Sang Hwa" sort="Lee, Sang Hwa" uniqKey="Lee S" first="Sang-Hwa" last="Lee">Sang-Hwa Lee</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Lim, Young Jin" sort="Lim, Young Jin" uniqKey="Lim Y" first="Young-Jin" last="Lim">Young-Jin Lim</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Yoo, Young Hyun" sort="Yoo, Young Hyun" uniqKey="Yoo Y" first="Young-Hyun" last="Yoo">Young-Hyun Yoo</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Kim, Jung Man" sort="Kim, Jung Man" uniqKey="Kim J" first="Jung-Man" last="Kim">Jung-Man Kim</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Lee, Jae Dong" sort="Lee, Jae Dong" uniqKey="Lee J" first="Jae-Dong" last="Lee">Jae-Dong Lee</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Jeong, Min Ho" sort="Jeong, Min Ho" uniqKey="Jeong M" first="Min-Ho" last="Jeong">Min-Ho Jeong</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">10948136</idno><idno type="pmc">101758</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC101758</idno><idno type="RBID">PMC:101758</idno><date when="2000">2000</date><idno type="wicri:Area/Pmc/Corpus">000A16</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000A16</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Novel Factor Isolated from <italic>Actinobacillus actinomycetemcomitans</italic> Stimulates Mouse B Cells and Human Peripheral Blood Mononuclear Cells</title><author><name sortKey="Jeong, Soo Jin" sort="Jeong, Soo Jin" uniqKey="Jeong S" first="Soo-Jin" last="Jeong">Soo-Jin Jeong</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Yee, Sung Tae" sort="Yee, Sung Tae" uniqKey="Yee S" first="Sung-Tae" last="Yee">Sung-Tae Yee</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Jo, Wol Soon" sort="Jo, Wol Soon" uniqKey="Jo W" first="Wol-Soon" last="Jo">Wol-Soon Jo</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Yu, Seong Ho" sort="Yu, Seong Ho" uniqKey="Yu S" first="Seong-Ho" last="Yu">Seong-Ho Yu</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Lee, Sang Hwa" sort="Lee, Sang Hwa" uniqKey="Lee S" first="Sang-Hwa" last="Lee">Sang-Hwa Lee</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Lim, Young Jin" sort="Lim, Young Jin" uniqKey="Lim Y" first="Young-Jin" last="Lim">Young-Jin Lim</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Yoo, Young Hyun" sort="Yoo, Young Hyun" uniqKey="Yoo Y" first="Young-Hyun" last="Yoo">Young-Hyun Yoo</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Kim, Jung Man" sort="Kim, Jung Man" uniqKey="Kim J" first="Jung-Man" last="Kim">Jung-Man Kim</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Lee, Jae Dong" sort="Lee, Jae Dong" uniqKey="Lee J" first="Jae-Dong" last="Lee">Jae-Dong Lee</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author><author><name sortKey="Jeong, Min Ho" sort="Jeong, Min Ho" uniqKey="Jeong M" first="Min-Ho" last="Jeong">Min-Ho Jeong</name><affiliation><nlm:aff id="N0x9b4d4f8.0x9c034b0"></nlm:aff></affiliation></author></analytic><series><title level="j">Infection and Immunity</title><idno type="ISSN">0019-9567</idno><idno type="eISSN">1098-5522</idno><imprint><date when="2000">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A novel immunostimulating factor (ISTF) of <italic>Actinobacillus actinomycetemcomitans</italic> ATCC 29522 was isolated and characterized as inducing proliferation of mouse B cells and human peripheral blood mononuclear cells. This factor was isolated from the bacterial culture medium and purified by size exclusion chromatography, dye-ligand affinity chromatography, immunoaffinity chromatography using monoclonal antibodies, and preparative electrophoresis. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the purified ISTF migrated as a single band corresponding to a molecular mass of 13 kDa. ISTF was a proteinaceous material distinct from lipopolysaccharide; it directly induced the proliferation of B lymphocytes but had no effect on the proliferation of T lymphocytes, even in the presence of antigen-presenting cells. A B-lymphocyte-mitogenic activity of ISTF was also shown by flow cytometric analysis of responding cell subpopulations. Immunoblot analysis revealed that ISTF was a component of the outer membranes of bacteria, could exist as a soluble form, and was released by growing and/or lysed bacteria. These results suggest that ISTF produced by <italic>A. actinomycetemcomitans</italic> may play an important role in immunopathologic changes associated with <italic>A. actinomycetemcomitans</italic> infections.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Infect Immun</journal-id><journal-id journal-id-type="publisher-id">INFECT IMMUN</journal-id><journal-title>Infection and Immunity</journal-title><issn pub-type="ppub">0019-9567</issn><issn pub-type="epub">1098-5522</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">10948136</article-id><article-id pub-id-type="pmc">101758</article-id><article-id pub-id-type="publisher-id">0253</article-id><article-categories><subj-group subj-group-type="heading"><subject>Bacterial Infections</subject></subj-group></article-categories><title-group><article-title>A Novel Factor Isolated from <italic>Actinobacillus actinomycetemcomitans</italic> Stimulates Mouse B Cells and Human Peripheral Blood Mononuclear Cells</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Jeong</surname><given-names>Soo-Jin</given-names></name><xref ref-type="aff" rid="N0x9b4d4f8.0x9c034b0">1</xref></contrib><contrib contrib-type="author"><name><surname>Yee</surname><given-names>Sung-Tae</given-names></name><xref ref-type="aff" rid="N0x9b4d4f8.0x9c034b0">2</xref></contrib><contrib contrib-type="author"><name><surname>Jo</surname><given-names>Wol-Soon</given-names></name><xref ref-type="aff" rid="N0x9b4d4f8.0x9c034b0">1</xref></contrib><contrib contrib-type="author"><name><surname>Yu</surname><given-names>Seong-Ho</given-names></name><xref ref-type="aff" rid="N0x9b4d4f8.0x9c034b0">1</xref></contrib><contrib contrib-type="author"><name><surname>Lee</surname><given-names>Sang-Hwa</given-names></name><xref ref-type="aff" rid="N0x9b4d4f8.0x9c034b0">1</xref></contrib><contrib contrib-type="author"><name><surname>Lim</surname><given-names>Young-Jin</given-names></name><xref ref-type="aff" rid="N0x9b4d4f8.0x9c034b0">1</xref></contrib><contrib contrib-type="author"><name><surname>Yoo</surname><given-names>Young-Hyun</given-names></name><xref ref-type="aff" rid="N0x9b4d4f8.0x9c034b0">1</xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Jung-Man</given-names></name><xref ref-type="aff" rid="N0x9b4d4f8.0x9c034b0">3</xref></contrib><contrib contrib-type="author"><name><surname>Lee</surname><given-names>Jae-Dong</given-names></name><xref ref-type="aff" rid="N0x9b4d4f8.0x9c034b0">4</xref></contrib><contrib contrib-type="author"><name><surname>Jeong</surname><given-names>Min-Ho</given-names></name><xref ref-type="aff" rid="N0x9b4d4f8.0x9c034b0">1</xref><xref ref-type="author-notes" rid="FN150">*</xref></contrib></contrib-group><aff id="N0x9b4d4f8.0x9c034b0"> The Institute of Medical Science<sup>1</sup> and Department of Clinical Pathology,<sup>3</sup> Dong-A University College of Medicine, and Department of Microbiology, Pusan National University,<sup>4</sup> Pusan, and Department of Biology, Sunchon National University, Sunchon,<sup>2</sup> Korea</aff><contrib-group><contrib contrib-type="editor"><name><surname>Kaufmann</surname><given-names>S. H. E.</given-names></name></contrib></contrib-group><author-notes><fn id="FN150"><label>*</label><p>Corresponding author. Mailing address: Department of Microbiology and Medical Science Institute, Dong-A University College of Medicine, Dongdaesindong, Seo-Gu, Pusan, South Korea 600-103. Phone: 82 51 240 2863. Fax: 82 51 242 9750. E-mail: <email>mhjeong@mail.donga.ac.kr</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>9</month><year>2000</year></pub-date><volume>68</volume><issue>9</issue><fpage>5132</fpage><lpage>5138</lpage><history><date date-type="received"><day>24</day><month>2</month><year>2000</year></date><date date-type="rev-request"><day>31</day><month>3</month><year>2000</year></date><date date-type="accepted"><day>15</day><month>6</month><year>2000</year></date></history><copyright-statement>Copyright © 2000, American Society for Microbiology</copyright-statement><copyright-year>2000</copyright-year><abstract><p>A novel immunostimulating factor (ISTF) of <italic>Actinobacillus actinomycetemcomitans</italic> ATCC 29522 was isolated and characterized as inducing proliferation of mouse B cells and human peripheral blood mononuclear cells. This factor was isolated from the bacterial culture medium and purified by size exclusion chromatography, dye-ligand affinity chromatography, immunoaffinity chromatography using monoclonal antibodies, and preparative electrophoresis. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the purified ISTF migrated as a single band corresponding to a molecular mass of 13 kDa. ISTF was a proteinaceous material distinct from lipopolysaccharide; it directly induced the proliferation of B lymphocytes but had no effect on the proliferation of T lymphocytes, even in the presence of antigen-presenting cells. A B-lymphocyte-mitogenic activity of ISTF was also shown by flow cytometric analysis of responding cell subpopulations. Immunoblot analysis revealed that ISTF was a component of the outer membranes of bacteria, could exist as a soluble form, and was released by growing and/or lysed bacteria. These results suggest that ISTF produced by <italic>A. actinomycetemcomitans</italic> may play an important role in immunopathologic changes associated with <italic>A. actinomycetemcomitans</italic> infections.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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