Bone Regeneration Using the Freshly Isolated Autologous Stromal Vascular Fraction of Adipose Tissue in Combination With Calcium Phosphate Ceramics
Identifieur interne : 000856 ( Pmc/Checkpoint ); précédent : 000855; suivant : 000857Bone Regeneration Using the Freshly Isolated Autologous Stromal Vascular Fraction of Adipose Tissue in Combination With Calcium Phosphate Ceramics
Auteurs : Henk-Jan Prins ; Engelbert A. J. M. Schulten ; Christiaan M. Ten Bruggenkate ; Jenneke Klein-Nulend ; Marco N. HelderSource :
- Stem Cells Translational Medicine [ 2157-6564 ] ; 2016.
Abstract
This phase I study evaluated the potential positive effect on bone regeneration by the addition of the freshly isolated, autologous but heterologous stromal vascular fraction (SVF) of adipose tissue. The study demonstrated for the first time the feasibility, safety, and potential efficacy of SVF seeded on bone substitutes for maxillary sinus floor elevation.
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DOI: 10.5966/sctm.2015-0369
PubMed: 27388241
PubMed Central: 5031181
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Bone Regeneration Using the Freshly Isolated Autologous Stromal Vascular Fraction of Adipose Tissue in Combination With Calcium Phosphate Ceramics</title><author><name sortKey="Prins, Henk Jan" sort="Prins, Henk Jan" uniqKey="Prins H" first="Henk-Jan" last="Prins">Henk-Jan Prins</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Schulten, Engelbert A J M" sort="Schulten, Engelbert A J M" uniqKey="Schulten E" first="Engelbert A. J. M." last="Schulten">Engelbert A. J. M. Schulten</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Ten Bruggenkate, Christiaan M" sort="Ten Bruggenkate, Christiaan M" uniqKey="Ten Bruggenkate C" first="Christiaan M." last="Ten Bruggenkate">Christiaan M. Ten Bruggenkate</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Klein Nulend, Jenneke" sort="Klein Nulend, Jenneke" uniqKey="Klein Nulend J" first="Jenneke" last="Klein-Nulend">Jenneke Klein-Nulend</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Helder, Marco N" sort="Helder, Marco N" uniqKey="Helder M" first="Marco N." last="Helder">Marco N. Helder</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27388241</idno><idno type="pmc">5031181</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5031181</idno><idno type="RBID">PMC:5031181</idno><idno type="doi">10.5966/sctm.2015-0369</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">002565</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002565</idno><idno type="wicri:Area/Pmc/Curation">002565</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002565</idno><idno type="wicri:Area/Pmc/Checkpoint">000856</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000856</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Bone Regeneration Using the Freshly Isolated Autologous Stromal Vascular Fraction of Adipose Tissue in Combination With Calcium Phosphate Ceramics</title><author><name sortKey="Prins, Henk Jan" sort="Prins, Henk Jan" uniqKey="Prins H" first="Henk-Jan" last="Prins">Henk-Jan Prins</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Schulten, Engelbert A J M" sort="Schulten, Engelbert A J M" uniqKey="Schulten E" first="Engelbert A. J. M." last="Schulten">Engelbert A. J. M. Schulten</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Ten Bruggenkate, Christiaan M" sort="Ten Bruggenkate, Christiaan M" uniqKey="Ten Bruggenkate C" first="Christiaan M." last="Ten Bruggenkate">Christiaan M. Ten Bruggenkate</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Klein Nulend, Jenneke" sort="Klein Nulend, Jenneke" uniqKey="Klein Nulend J" first="Jenneke" last="Klein-Nulend">Jenneke Klein-Nulend</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Helder, Marco N" sort="Helder, Marco N" uniqKey="Helder M" first="Marco N." last="Helder">Marco N. Helder</name><affiliation><nlm:aff id="aff3"></nlm:aff></affiliation></author></analytic><series><title level="j">Stem Cells Translational Medicine</title><idno type="ISSN">2157-6564</idno><idno type="eISSN">2157-6580</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>This phase I study evaluated the potential positive effect on bone regeneration by the addition of the freshly isolated, autologous but heterologous stromal vascular fraction (SVF) of adipose tissue. The study demonstrated for the first time the feasibility, safety, and potential efficacy of SVF seeded on bone substitutes for maxillary sinus floor elevation.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Stem Cells Transl Med</journal-id><journal-id journal-id-type="iso-abbrev">Stem Cells Transl Med</journal-id><journal-id journal-id-type="pmc">Stem Cells Translational Medicine</journal-id><journal-id journal-id-type="hwp">sctm</journal-id><journal-id journal-id-type="publisher-id">Stem Cells Translational Medicine</journal-id><journal-title-group><journal-title>Stem Cells Translational Medicine</journal-title></journal-title-group><issn pub-type="ppub">2157-6564</issn><issn pub-type="epub">2157-6580</issn><publisher><publisher-name>AlphaMed Press</publisher-name><publisher-loc>Durham, NC, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27388241</article-id><article-id pub-id-type="pmc">5031181</article-id><article-id pub-id-type="publisher-id">20150369</article-id><article-id pub-id-type="doi">10.5966/sctm.2015-0369</article-id><article-categories><subj-group subj-group-type="hwp-journal-coll"><subject>11</subject><subject>47</subject><subject>14</subject><subject>35</subject></subj-group><subj-group subj-group-type="heading"><subject>Tissue Engineering and Regenerative Medicine</subject></subj-group></article-categories><title-group><article-title>Bone Regeneration Using the Freshly Isolated Autologous Stromal Vascular Fraction of Adipose Tissue in Combination With Calcium Phosphate Ceramics</article-title><alt-title alt-title-type="short">Bone Regeneration With Autologous Fat Stem Cells</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Prins</surname><given-names>Henk-Jan</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="aff" rid="aff2"><sup>b</sup></xref><xref ref-type="author-notes" rid="afn1">*</xref></contrib><contrib contrib-type="author"><name><surname>Schulten</surname><given-names>Engelbert A.J.M.</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref><xref ref-type="author-notes" rid="afn1">*</xref></contrib><contrib contrib-type="author"><name><surname>ten Bruggenkate</surname><given-names>Christiaan M.</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Klein-Nulend</surname><given-names>Jenneke</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="author-notes" rid="afn2"><sup>†</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Helder</surname><given-names>Marco N.</given-names></name><xref ref-type="aff" rid="aff3"><sup>c</sup></xref><xref ref-type="author-notes" rid="afn2"><sup>†</sup></xref></contrib><aff id="aff1"><label><sup>a</sup></label>Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), MOVE Research Institute Amsterdam, VU University Amsterdam, Amsterdam, The Netherlands</aff><aff id="aff2"><label><sup>b</sup></label>Department of Oral and Maxillofacial Surgery, MOVE Research Institute Amsterdam, VU University Medical Center/ACTA, Amsterdam, The Netherlands</aff><aff id="aff3"><label><sup>c</sup></label>Department of Orthopedic Surgery, MOVE Research Institute Amsterdam, VU University Medical Center, Amsterdam, The Netherlands</aff></contrib-group><author-notes><fn fn-type="equal" id="afn1"><label>*</label><p>Contributed equally as primary authors.</p></fn><fn fn-type="equal" id="afn2"><label><sup>†</sup></label><p>Contributed equally as last authors.</p></fn><corresp id="cor1">Correspondence: Marco N. Helder, Ph.D., Department of Orthopedic Surgery, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands. Telephone: <phone>31 204445024</phone>; E-mail: <email>m.helder@vumc.nl</email></corresp></author-notes><pub-date pub-type="ppub"><month>10</month><year>2016</year></pub-date><pub-date pub-type="epub"><day>07</day><month>7</month><year>2016</year></pub-date><pub-date pub-type="pmc-release"><day>1</day><month>10</month><year>2017</year></pub-date><pmc-comment> PMC Release delay is 12 months and
0 days and was based on the
<volume>5</volume><issue>10</issue><fpage>1362</fpage><lpage>1374</lpage><history><date date-type="received"><day>02</day><month>12</month><year>2015</year></date><date date-type="accepted"><day>18</day><month>4</month><year>2016</year></date></history><permissions><copyright-statement>©AlphaMed Press</copyright-statement><copyright-year>2016</copyright-year></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="sctm_20150369.pdf"></self-uri><abstract abstract-type="precis"><p>This phase I study evaluated the potential positive effect on bone regeneration by the addition of the freshly isolated, autologous but heterologous stromal vascular fraction (SVF) of adipose tissue. The study demonstrated for the first time the feasibility, safety, and potential efficacy of SVF seeded on bone substitutes for maxillary sinus floor elevation.</p></abstract><abstract><sec><title></title><p>In patients undergoing maxillary sinus floor elevation (MSFE) for dental implant placement, bone substitutes are currently evaluated as alternatives for autologous bone. However, bone substitutes have only osteoconductive properties and lack osteoinductive potential. Therefore, this phase I study evaluated the potential additive effect on bone regeneration by the addition of freshly isolated, autologous but heterologous stromal vascular fraction (SVF), which is highly enriched with adipose stromal/stem cells when compared with native adipose tissue. From 10 patients, SVF was procured using automatic processing, seeded on either β-tricalcium phosphate (<italic>n</italic> = 5) or biphasic calcium phosphate carriers (<italic>n</italic> = 5), and used for MSFE in a one-step surgical procedure. Primary objectives were feasibility and safety. The secondary objective was efficacy, evaluated by using biopsies of the augmented area taken 6 months postoperatively, concomitant with dental implant placement. Biopsies were assessed for bone, graft, and osteoid volumes. No adverse effects were reported during the procedure or follow-up (≥3 years). Bone and osteoid percentages were higher in study biopsies (SVF supplemented) than in control biopsies (ceramic only on contralateral side), in particular in β-tricalcium phosphate-treated patients. Paired analysis on the six bilaterally treated patients revealed markedly higher bone and osteoid volumes using microcomputed tomography or histomorphometric evaluations, demonstrating an additive effect of SVF supplementation, independent of the bone substitute. This study demonstrated for the first time the feasibility, safety, and potential efficacy of SVF seeded on bone substitutes for MSFE, providing the first step toward a novel treatment concept that might offer broad potential for SVF-based regenerative medicine applications.</p></sec><sec><title>Significance</title><p>This is the first-in-human study using freshly isolated, autologous adipose stem cell preparations (the stromal vascular fraction [SVF] of adipose tissue) applied in a one-step surgical procedure with calcium phosphate ceramics (CaP) to increase maxillary bone height for dental implantations. All 10 patients received CaP plus SVF on one side, whereas bilaterally treated patients (6 of 10) received CaP only on the opposite side. This allowed intrapatient evaluation of the potential added value of SVF supplementation, assessed in biopsies obtained after 6 months. Feasibility, safety, and potential efficacy of SVF for bone regeneration were demonstrated, showing high potential for this novel concept.</p></sec></abstract><kwd-group><kwd>Bone regeneration</kwd><kwd>Clinical study</kwd><kwd>Calcium phosphates</kwd><kwd>Maxillary sinus floor elevation</kwd><kwd>Adipose stem cells</kwd><kwd>Regenerative medicine</kwd></kwd-group><counts><fig-count count="5"></fig-count><table-count count="2"></table-count><equation-count count="0"></equation-count><ref-count count="49"></ref-count><page-count count="13"></page-count></counts><custom-meta-group><custom-meta><meta-name> DJS Export </meta-name><meta-value>v1</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Helder, Marco N" sort="Helder, Marco N" uniqKey="Helder M" first="Marco N." last="Helder">Marco N. Helder</name><name sortKey="Klein Nulend, Jenneke" sort="Klein Nulend, Jenneke" uniqKey="Klein Nulend J" first="Jenneke" last="Klein-Nulend">Jenneke Klein-Nulend</name><name sortKey="Prins, Henk Jan" sort="Prins, Henk Jan" uniqKey="Prins H" first="Henk-Jan" last="Prins">Henk-Jan Prins</name><name sortKey="Schulten, Engelbert A J M" sort="Schulten, Engelbert A J M" uniqKey="Schulten E" first="Engelbert A. J. M." last="Schulten">Engelbert A. J. M. Schulten</name><name sortKey="Ten Bruggenkate, Christiaan M" sort="Ten Bruggenkate, Christiaan M" uniqKey="Ten Bruggenkate C" first="Christiaan M." last="Ten Bruggenkate">Christiaan M. Ten Bruggenkate</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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