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Detection of Porphyromonas gingivalis from Saliva by PCR by Using a Simple Sample-Processing Method

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Detection of Porphyromonas gingivalis from Saliva by PCR by Using a Simple Sample-Processing Method

Auteurs : Jaana M Ttö ; Maria Saarela ; Satu Alaluusua ; Virva Oja ; Hannele Jousimies-Somer ; Sirkka Asikainen

Source :

Journal of Clinical Microbiology [ 0095-1137 ] ; 1998.

RBID : PMC:124827

Abstract

Simple sample-processing methods for PCR detection of Porphyromonas gingivalis, a major pathogen causing adult periodontitis, from saliva were studied. The ability to detect P. gingivalis from 118 salivary samples by PCR after boiling and Chelex 100 processing was compared with bacterial culture. P. gingivalis was detected three times more often by PCR than by culture. Chelex 100 processing of saliva proved to be effective in preventing PCR inhibition and was applied to determine the occurrence of P. gingivalis in saliva samples from 263 Finnish subjects between 5 and 80 years of age. The occurrence of P. gingivalis increased with age, and it was detected by PCR in the saliva of 5.0% of subjects between 5 and 10 years of age, 13.8% of subjects between 11 and 20 years of age, 13.4% of subjects between 21 and 30 years of age, and 63.3% of subjects between 31 and 80 years of age. The results indicate that P. gingivalis is a rare finding in saliva from periodontally healthy children and young adults but a frequent one in saliva from adult periodontitis patients.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124827

PubMed: 9431940
PubMed Central: 124827

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PMC:124827

Le document en format XML

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<author><name sortKey="Jousimies Somer, Hannele" sort="Jousimies Somer, Hannele" uniqKey="Jousimies Somer H" first="Hannele" last="Jousimies-Somer">Hannele Jousimies-Somer</name>
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<author><name sortKey="Alaluusua, Satu" sort="Alaluusua, Satu" uniqKey="Alaluusua S" first="Satu" last="Alaluusua">Satu Alaluusua</name>
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<author><name sortKey="Oja, Virva" sort="Oja, Virva" uniqKey="Oja V" first="Virva" last="Oja">Virva Oja</name>
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<author><name sortKey="Jousimies Somer, Hannele" sort="Jousimies Somer, Hannele" uniqKey="Jousimies Somer H" first="Hannele" last="Jousimies-Somer">Hannele Jousimies-Somer</name>
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<author><name sortKey="Asikainen, Sirkka" sort="Asikainen, Sirkka" uniqKey="Asikainen S" first="Sirkka" last="Asikainen">Sirkka Asikainen</name>
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<series><title level="j">Journal of Clinical Microbiology</title>
<idno type="ISSN">0095-1137</idno>
<idno type="eISSN">1098-660X</idno>
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<front><div type="abstract" xml:lang="en"><p>Simple sample-processing methods for PCR detection of <italic>Porphyromonas gingivalis</italic>
, a major pathogen causing adult periodontitis, from saliva were studied. The ability to detect <italic>P. gingivalis</italic>
 from 118 salivary samples by PCR after boiling and Chelex 100 processing was compared with bacterial culture. <italic>P. gingivalis</italic>
 was detected three times more often by PCR than by culture. Chelex 100 processing of saliva proved to be effective in preventing PCR inhibition and was applied to determine the occurrence of <italic>P. gingivalis</italic>
 in saliva samples from 263 Finnish subjects between 5 and 80 years of age. The occurrence of <italic>P. gingivalis</italic>
 increased with age, and it was detected by PCR in the saliva of 5.0% of subjects between 5 and 10 years of age, 13.8% of subjects between 11 and 20 years of age, 13.4% of subjects between 21 and 30 years of age, and 63.3% of subjects between 31 and 80 years of age. The results indicate that <italic>P. gingivalis</italic>
 is a rare finding in saliva from periodontally healthy children and young adults but a frequent one in saliva from adult periodontitis patients.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">J Clin Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">J CLIN MICROBIOL</journal-id>
<journal-title>Journal of Clinical Microbiology</journal-title>
<issn pub-type="ppub">0095-1137</issn>
<issn pub-type="epub">1098-660X</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
</publisher>
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<article-meta><article-id pub-id-type="pmid">9431940</article-id>
<article-id pub-id-type="pmc">124827</article-id>
<article-id pub-id-type="publisher-id">0420</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Bacteriology</subject>
</subj-group>
</article-categories>
<title-group><article-title>Detection of <italic>Porphyromonas gingivalis</italic>
 from Saliva by PCR by Using a Simple Sample-Processing Method</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Mättö</surname>
<given-names>Jaana</given-names>
</name>
<xref ref-type="aff" rid="N0x97b79e8.0x9d98128">1</xref>
<xref ref-type="author-notes" rid="FN150">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Saarela</surname>
<given-names>Maria</given-names>
</name>
<xref ref-type="aff" rid="N0x97b79e8.0x9d98128">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Alaluusua</surname>
<given-names>Satu</given-names>
</name>
<xref ref-type="aff" rid="N0x97b79e8.0x9d98128">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Oja</surname>
<given-names>Virva</given-names>
</name>
<xref ref-type="aff" rid="N0x97b79e8.0x9d98128">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jousimies-Somer</surname>
<given-names>Hannele</given-names>
</name>
<xref ref-type="aff" rid="N0x97b79e8.0x9d98128">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Asikainen</surname>
<given-names>Sirkka</given-names>
</name>
<xref ref-type="aff" rid="N0x97b79e8.0x9d98128">1</xref>
<xref ref-type="aff" rid="N0x97b79e8.0x9d98128">4</xref>
</contrib>
</contrib-group>
<aff id="N0x97b79e8.0x9d98128"> Research Laboratory, Institute of Dentistry,<sup>1</sup>
 Department of Pedodontics and Orthodontics,<sup>2</sup>
 and Department of Periodontology,<sup>4</sup>
 University of Helsinki, and Anaerobe Reference Laboratory, National Public Health Institute,<sup>3</sup>
 Helsinki, Finland</aff>
<author-notes><fn id="FN150"><label>*</label>
<p>Corresponding author. Mailing address: Institute of Dentistry, Research Laboratory, P.O. Box 41, FIN-00014 University of Helsinki, Finland. Phone: 358-9-19127316. Fax: 358-9-19127519.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>1</month>
<year>1998</year>
</pub-date>
<volume>36</volume>
<issue>1</issue>
<fpage>157</fpage>
<lpage>160</lpage>
<history><date date-type="received"><day>24</day>
<month>4</month>
<year>1997</year>
</date>
<date date-type="rev-request"><day>16</day>
<month>7</month>
<year>1997</year>
</date>
<date date-type="accepted"><day>1</day>
<month>10</month>
<year>1997</year>
</date>
</history>
<copyright-statement>Copyright © 1998, American Society for Microbiology</copyright-statement>
<copyright-year>1998</copyright-year>
<abstract><p>Simple sample-processing methods for PCR detection of <italic>Porphyromonas gingivalis</italic>
, a major pathogen causing adult periodontitis, from saliva were studied. The ability to detect <italic>P. gingivalis</italic>
 from 118 salivary samples by PCR after boiling and Chelex 100 processing was compared with bacterial culture. <italic>P. gingivalis</italic>
 was detected three times more often by PCR than by culture. Chelex 100 processing of saliva proved to be effective in preventing PCR inhibition and was applied to determine the occurrence of <italic>P. gingivalis</italic>
 in saliva samples from 263 Finnish subjects between 5 and 80 years of age. The occurrence of <italic>P. gingivalis</italic>
 increased with age, and it was detected by PCR in the saliva of 5.0% of subjects between 5 and 10 years of age, 13.8% of subjects between 11 and 20 years of age, 13.4% of subjects between 21 and 30 years of age, and 63.3% of subjects between 31 and 80 years of age. The results indicate that <italic>P. gingivalis</italic>
 is a rare finding in saliva from periodontally healthy children and young adults but a frequent one in saliva from adult periodontitis patients.</p>
</abstract>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Alaluusua, Satu" sort="Alaluusua, Satu" uniqKey="Alaluusua S" first="Satu" last="Alaluusua">Satu Alaluusua</name>
<name sortKey="Asikainen, Sirkka" sort="Asikainen, Sirkka" uniqKey="Asikainen S" first="Sirkka" last="Asikainen">Sirkka Asikainen</name>
<name sortKey="Jousimies Somer, Hannele" sort="Jousimies Somer, Hannele" uniqKey="Jousimies Somer H" first="Hannele" last="Jousimies-Somer">Hannele Jousimies-Somer</name>
<name sortKey="M Tto, Jaana" sort="M Tto, Jaana" uniqKey="M Tto J" first="Jaana" last="M Ttö">Jaana M Ttö</name>
<name sortKey="Oja, Virva" sort="Oja, Virva" uniqKey="Oja V" first="Virva" last="Oja">Virva Oja</name>
<name sortKey="Saarela, Maria" sort="Saarela, Maria" uniqKey="Saarela M" first="Maria" last="Saarela">Maria Saarela</name>
</noCountry>
</tree>
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Detection of Porphyromonas gingivalis from Saliva by PCR by Using a Simple Sample-Processing Method

Detection of Porphyromonas gingivalis from Saliva by PCR by Using a Simple Sample-Processing Method

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Abstract

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