Soluble factors from biofilm of Candida albicans and Staphylococcus aureus promote cell death and inflammatory response
Identifieur interne : 007062 ( Ncbi/Merge ); précédent : 007061; suivant : 007063Soluble factors from biofilm of Candida albicans and Staphylococcus aureus promote cell death and inflammatory response
Auteurs : Kassia De Carvalho Dias ; Paula Aboud Barbugli ; Fernanda De Patto ; Virginia Barreto Lordello ; Letícia De Aquino Penteado ; Alexandra Ivo Medeiros ; Carlos Eduardo VerganiSource :
- BMC Microbiology [ 1471-2180 ] ; 2017.
Abstract
The objective of this study was to better understand the effects of soluble factors from biofilm of single- and mixed-species
The cell viability assays showed that the soluble factors of single-species
Soluble factors from 36-h-old biofilm of
Url:
DOI: 10.1186/s12866-017-1031-5
PubMed: 28666415
PubMed Central: 5493077
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Soluble factors from biofilm of <italic>Candida albicans</italic> and <italic>Staphylococcus aureus</italic> promote cell death and inflammatory response</title><author><name sortKey="De Carvalho Dias, Kassia" sort="De Carvalho Dias, Kassia" uniqKey="De Carvalho Dias K" first="Kassia" last="De Carvalho Dias">Kassia De Carvalho Dias</name><affiliation><nlm:aff id="Aff1">Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program–Araraquara School of Dentistry UNESP–Univ. Estadual Paulista, Centro, Araraquara, SP 14801903 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 14801903 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Barbugli, Paula Aboud" sort="Barbugli, Paula Aboud" uniqKey="Barbugli P" first="Paula Aboud" last="Barbugli">Paula Aboud Barbugli</name><affiliation><nlm:aff id="Aff1">Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program–Araraquara School of Dentistry UNESP–Univ. Estadual Paulista, Centro, Araraquara, SP 14801903 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 14801903 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="De Patto, Fernanda" sort="De Patto, Fernanda" uniqKey="De Patto F" first="Fernanda" last="De Patto">Fernanda De Patto</name><affiliation><nlm:aff id="Aff1">Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program–Araraquara School of Dentistry UNESP–Univ. Estadual Paulista, Centro, Araraquara, SP 14801903 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 14801903 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Lordello, Virginia Barreto" sort="Lordello, Virginia Barreto" uniqKey="Lordello V" first="Virginia Barreto" last="Lordello">Virginia Barreto Lordello</name><affiliation><nlm:aff id="Aff2">Department of Biological Sciences, School of Pharmaceutical Sciences, UNESP–Univ Estadual Paulista, Araraquara, São Paulo Brazil</nlm:aff><wicri:noCountry code="subfield">São Paulo Brazil</wicri:noCountry></affiliation></author><author><name sortKey="De Aquino Penteado, Leticia" sort="De Aquino Penteado, Leticia" uniqKey="De Aquino Penteado L" first="Letícia" last="De Aquino Penteado">Letícia De Aquino Penteado</name><affiliation><nlm:aff id="Aff2">Department of Biological Sciences, School of Pharmaceutical Sciences, UNESP–Univ Estadual Paulista, Araraquara, São Paulo Brazil</nlm:aff><wicri:noCountry code="subfield">São Paulo Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Medeiros, Alexandra Ivo" sort="Medeiros, Alexandra Ivo" uniqKey="Medeiros A" first="Alexandra Ivo" last="Medeiros">Alexandra Ivo Medeiros</name><affiliation><nlm:aff id="Aff2">Department of Biological Sciences, School of Pharmaceutical Sciences, UNESP–Univ Estadual Paulista, Araraquara, São Paulo Brazil</nlm:aff><wicri:noCountry code="subfield">São Paulo Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Vergani, Carlos Eduardo" sort="Vergani, Carlos Eduardo" uniqKey="Vergani C" first="Carlos Eduardo" last="Vergani">Carlos Eduardo Vergani</name><affiliation><nlm:aff id="Aff1">Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program–Araraquara School of Dentistry UNESP–Univ. Estadual Paulista, Centro, Araraquara, SP 14801903 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 14801903 Brazil</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28666415</idno><idno type="pmc">5493077</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5493077</idno><idno type="RBID">PMC:5493077</idno><idno type="doi">10.1186/s12866-017-1031-5</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000B78</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000B78</idno><idno type="wicri:Area/Pmc/Curation">000B78</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000B78</idno><idno type="wicri:Area/Pmc/Checkpoint">000056</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000056</idno><idno type="wicri:Area/Ncbi/Merge">007062</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Soluble factors from biofilm of <italic>Candida albicans</italic> and <italic>Staphylococcus aureus</italic> promote cell death and inflammatory response</title><author><name sortKey="De Carvalho Dias, Kassia" sort="De Carvalho Dias, Kassia" uniqKey="De Carvalho Dias K" first="Kassia" last="De Carvalho Dias">Kassia De Carvalho Dias</name><affiliation><nlm:aff id="Aff1">Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program–Araraquara School of Dentistry UNESP–Univ. Estadual Paulista, Centro, Araraquara, SP 14801903 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 14801903 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Barbugli, Paula Aboud" sort="Barbugli, Paula Aboud" uniqKey="Barbugli P" first="Paula Aboud" last="Barbugli">Paula Aboud Barbugli</name><affiliation><nlm:aff id="Aff1">Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program–Araraquara School of Dentistry UNESP–Univ. Estadual Paulista, Centro, Araraquara, SP 14801903 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 14801903 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="De Patto, Fernanda" sort="De Patto, Fernanda" uniqKey="De Patto F" first="Fernanda" last="De Patto">Fernanda De Patto</name><affiliation><nlm:aff id="Aff1">Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program–Araraquara School of Dentistry UNESP–Univ. Estadual Paulista, Centro, Araraquara, SP 14801903 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 14801903 Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Lordello, Virginia Barreto" sort="Lordello, Virginia Barreto" uniqKey="Lordello V" first="Virginia Barreto" last="Lordello">Virginia Barreto Lordello</name><affiliation><nlm:aff id="Aff2">Department of Biological Sciences, School of Pharmaceutical Sciences, UNESP–Univ Estadual Paulista, Araraquara, São Paulo Brazil</nlm:aff><wicri:noCountry code="subfield">São Paulo Brazil</wicri:noCountry></affiliation></author><author><name sortKey="De Aquino Penteado, Leticia" sort="De Aquino Penteado, Leticia" uniqKey="De Aquino Penteado L" first="Letícia" last="De Aquino Penteado">Letícia De Aquino Penteado</name><affiliation><nlm:aff id="Aff2">Department of Biological Sciences, School of Pharmaceutical Sciences, UNESP–Univ Estadual Paulista, Araraquara, São Paulo Brazil</nlm:aff><wicri:noCountry code="subfield">São Paulo Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Medeiros, Alexandra Ivo" sort="Medeiros, Alexandra Ivo" uniqKey="Medeiros A" first="Alexandra Ivo" last="Medeiros">Alexandra Ivo Medeiros</name><affiliation><nlm:aff id="Aff2">Department of Biological Sciences, School of Pharmaceutical Sciences, UNESP–Univ Estadual Paulista, Araraquara, São Paulo Brazil</nlm:aff><wicri:noCountry code="subfield">São Paulo Brazil</wicri:noCountry></affiliation></author><author><name sortKey="Vergani, Carlos Eduardo" sort="Vergani, Carlos Eduardo" uniqKey="Vergani C" first="Carlos Eduardo" last="Vergani">Carlos Eduardo Vergani</name><affiliation><nlm:aff id="Aff1">Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program–Araraquara School of Dentistry UNESP–Univ. Estadual Paulista, Centro, Araraquara, SP 14801903 Brazil</nlm:aff><wicri:noCountry code="subfield">SP 14801903 Brazil</wicri:noCountry></affiliation></author></analytic><series><title level="j">BMC Microbiology</title><idno type="eISSN">1471-2180</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p id="Par1">The objective of this study was to better understand the effects of soluble factors from biofilm of single- and mixed-species <italic>Candida albicans</italic> (<italic>C. albicans)</italic> and methicillin-sensitive <italic>Staphylococcus aureus</italic> (MSSA) cultures after 36 h in culture on keratinocytes (NOK-si and HaCaT) and macrophages (J774A.1). Soluble factors from biofilms of <italic>C. albicans</italic> and MSSA were collected and incubated with keratinocytes and macrophages, which were subsequently evaluated by cell viability assays (MTT). Lactate dehydrogenase (LDH) enzyme release was measured to assess cell membrane damage to keratinocytes. Cells were analysed by brightfield microscopy after 2 and 24 h of exposure to the soluble factors from biofilm. Cell death was detected by labelling apoptotic cells with annexin V and necrotic cells with propidium iodide (PI) and was visualized via fluorescence microscopy. Soluble factors from biofilm were incubated with J774A.1 cells for 24 h; the subsequent production of NO and the cytokines IL-6 and TNF-α was measured by ELISA.</p></sec><sec><title>Results</title><p id="Par2">The cell viability assays showed that the soluble factors of single-species <italic>C. albicans</italic> cultures were as toxic as the soluble factors from biofilm of mixed cultures, whereas the soluble factors of MSSA cultures were less toxic than those of <italic>C. albicans</italic> or mixed cultures. The soluble factors from biofilm of mixed cultures were the most toxic to the NOK-si and HaCaT cells, as confirmed by analyses of PI labelling and cell morphology. Soluble factors from biofilm of single-species MSSA and mixed-species cultures induced the production of IL-6, NO and TNF-α by J744A.1 macrophages. The production of IL-6 and NO induced by the soluble factors from biofilm of mixed cultures was lower than that induced by the soluble factors from biofilm of single-species MSSA cultures, whereas the soluble factors from biofilm of <italic>C. albicans</italic> cultures induced only low levels of NO.</p></sec><sec><title>Conclusions</title><p id="Par3">Soluble factors from 36-h-old biofilm of <italic>C. albicans</italic> and MSSA cultures promoted cell death and inflammatory responses.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Socransky, Ss" uniqKey="Socransky S">SS Socransky</name></author><author><name sortKey="Haffajee, Ad" uniqKey="Haffajee A">AD Haffajee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nobile, Cj" uniqKey="Nobile C">CJ Nobile</name></author><author><name sortKey="Mitchell, Ap" uniqKey="Mitchell A">AP 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<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Microbiol</journal-id><journal-id journal-id-type="iso-abbrev">BMC Microbiol</journal-id><journal-title-group><journal-title>BMC Microbiology</journal-title></journal-title-group><issn pub-type="epub">1471-2180</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28666415</article-id><article-id pub-id-type="pmc">5493077</article-id><article-id pub-id-type="publisher-id">1031</article-id><article-id pub-id-type="doi">10.1186/s12866-017-1031-5</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Soluble factors from biofilm of <italic>Candida albicans</italic> and <italic>Staphylococcus aureus</italic> promote cell death and inflammatory response</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>de Carvalho Dias</surname><given-names>Kassia</given-names></name><address><email>kassiaodonto@hotmail.com</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Barbugli</surname><given-names>Paula Aboud</given-names></name><address><email>barbugli@foar.unesp.br</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>de Patto</surname><given-names>Fernanda</given-names></name><address><email>fernandafoar@unesp.br</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Lordello</surname><given-names>Virginia Barreto</given-names></name><address><email>vii_lordello@hotmail.com</email></address><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>de Aquino Penteado</surname><given-names>Letícia</given-names></name><address><email>leticia_penteado@hotmail.com</email></address><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Medeiros</surname><given-names>Alexandra Ivo</given-names></name><address><email>medeirosai@fcfar.unesp.br</email></address><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0002-7375-4714</contrib-id><name><surname>Vergani</surname><given-names>Carlos Eduardo</given-names></name><address><email>vergani@foar.unesp.br</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label>Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program–Araraquara School of Dentistry UNESP–Univ. Estadual Paulista, Centro, Araraquara, SP 14801903 Brazil</aff><aff id="Aff2"><label>2</label>Department of Biological Sciences, School of Pharmaceutical Sciences, UNESP–Univ Estadual Paulista, Araraquara, São Paulo Brazil</aff></contrib-group><pub-date pub-type="epub"><day>30</day><month>6</month><year>2017</year></pub-date><pub-date pub-type="pmc-release"><day>30</day><month>6</month><year>2017</year></pub-date><pub-date pub-type="collection"><year>2017</year></pub-date><volume>17</volume><elocation-id>146</elocation-id><history><date date-type="received"><day>31</day><month>1</month><year>2017</year></date><date date-type="accepted"><day>15</day><month>5</month><year>2017</year></date></history><permissions><copyright-statement>© The Author(s). 2017</copyright-statement><license license-type="OpenAccess"><license-p><bold>Open Access</bold>This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>) applies to the data made available in this article, unless otherwise stated.</license-p></license></permissions><abstract id="Abs1"><sec><title>Background</title><p id="Par1">The objective of this study was to better understand the effects of soluble factors from biofilm of single- and mixed-species <italic>Candida albicans</italic> (<italic>C. albicans)</italic> and methicillin-sensitive <italic>Staphylococcus aureus</italic> (MSSA) cultures after 36 h in culture on keratinocytes (NOK-si and HaCaT) and macrophages (J774A.1). Soluble factors from biofilms of <italic>C. albicans</italic> and MSSA were collected and incubated with keratinocytes and macrophages, which were subsequently evaluated by cell viability assays (MTT). Lactate dehydrogenase (LDH) enzyme release was measured to assess cell membrane damage to keratinocytes. Cells were analysed by brightfield microscopy after 2 and 24 h of exposure to the soluble factors from biofilm. Cell death was detected by labelling apoptotic cells with annexin V and necrotic cells with propidium iodide (PI) and was visualized via fluorescence microscopy. Soluble factors from biofilm were incubated with J774A.1 cells for 24 h; the subsequent production of NO and the cytokines IL-6 and TNF-α was measured by ELISA.</p></sec><sec><title>Results</title><p id="Par2">The cell viability assays showed that the soluble factors of single-species <italic>C. albicans</italic> cultures were as toxic as the soluble factors from biofilm of mixed cultures, whereas the soluble factors of MSSA cultures were less toxic than those of <italic>C. albicans</italic> or mixed cultures. The soluble factors from biofilm of mixed cultures were the most toxic to the NOK-si and HaCaT cells, as confirmed by analyses of PI labelling and cell morphology. Soluble factors from biofilm of single-species MSSA and mixed-species cultures induced the production of IL-6, NO and TNF-α by J744A.1 macrophages. The production of IL-6 and NO induced by the soluble factors from biofilm of mixed cultures was lower than that induced by the soluble factors from biofilm of single-species MSSA cultures, whereas the soluble factors from biofilm of <italic>C. albicans</italic> cultures induced only low levels of NO.</p></sec><sec><title>Conclusions</title><p id="Par3">Soluble factors from 36-h-old biofilm of <italic>C. albicans</italic> and MSSA cultures promoted cell death and inflammatory responses.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Biofilm</kwd><kwd>Cytokines</kwd><kwd><italic>Candida albicans</italic></kwd><kwd><italic>Staphylococcus aureus</italic></kwd><kwd>Keratinocytes</kwd><kwd>Macrophages</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© The Author(s) 2017</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Barbugli, Paula Aboud" sort="Barbugli, Paula Aboud" uniqKey="Barbugli P" first="Paula Aboud" last="Barbugli">Paula Aboud Barbugli</name><name sortKey="De Aquino Penteado, Leticia" sort="De Aquino Penteado, Leticia" uniqKey="De Aquino Penteado L" first="Letícia" last="De Aquino Penteado">Letícia De Aquino Penteado</name><name sortKey="De Carvalho Dias, Kassia" sort="De Carvalho Dias, Kassia" uniqKey="De Carvalho Dias K" first="Kassia" last="De Carvalho Dias">Kassia De Carvalho Dias</name><name sortKey="De Patto, Fernanda" sort="De Patto, Fernanda" uniqKey="De Patto F" first="Fernanda" last="De Patto">Fernanda De Patto</name><name sortKey="Lordello, Virginia Barreto" sort="Lordello, Virginia Barreto" uniqKey="Lordello V" first="Virginia Barreto" last="Lordello">Virginia Barreto Lordello</name><name sortKey="Medeiros, Alexandra Ivo" sort="Medeiros, Alexandra Ivo" uniqKey="Medeiros A" first="Alexandra Ivo" last="Medeiros">Alexandra Ivo Medeiros</name><name sortKey="Vergani, Carlos Eduardo" sort="Vergani, Carlos Eduardo" uniqKey="Vergani C" first="Carlos Eduardo" last="Vergani">Carlos Eduardo Vergani</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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