Reproducibility of microscopic and cultural data in repeated subgingival plaque samples.
Identifieur interne : 006A65 ( Ncbi/Merge ); précédent : 006A64; suivant : 006A66Reproducibility of microscopic and cultural data in repeated subgingival plaque samples.
Auteurs : A. Mombelli [Suisse] ; C E Minder ; F A Gusberti ; N P LangSource :
- Journal of clinical periodontology [ 0303-6979 ] ; 1989.
Descripteurs français
- KwdFr :
- Bacteroides (isolement et purification), Bactéries (), Bactéries (isolement et purification), Bactéries à Gram négatif (isolement et purification), Bactéries à Gram positif (isolement et purification), Facteurs temps, Gencive, Humains, Maladies parodontales (microbiologie), Microscopie (), Mâchoire édentée (microbiologie), Plaque dentaire (microbiologie), Pose d'implant dentaire endo-osseux, Spirochaetales (isolement et purification).
- MESH :
- isolement et purification : Bacteroides, Bactéries, Bactéries à Gram négatif, Bactéries à Gram positif, Spirochaetales.
- microbiologie : Maladies parodontales, Mâchoire édentée, Plaque dentaire.
- Bactéries, Facteurs temps, Gencive, Humains, Microscopie, Pose d'implant dentaire endo-osseux.
English descriptors
- KwdEn :
- Bacteria (classification), Bacteria (isolation & purification), Bacteroides (isolation & purification), Dental Implantation, Endosseous, Dental Plaque (microbiology), Gingiva, Gram-Negative Bacteria (isolation & purification), Gram-Positive Bacteria (isolation & purification), Humans, Jaw, Edentulous (microbiology), Microscopy (methods), Periodontal Diseases (microbiology), Spirochaetales (isolation & purification), Time Factors.
- MESH :
- classification : Bacteria.
- isolation & purification : Bacteria, Bacteroides, Gram-Negative Bacteria, Gram-Positive Bacteria, Spirochaetales.
- methods : Microscopy.
- microbiology : Dental Plaque, Jaw, Edentulous, Periodontal Diseases.
- Dental Implantation, Endosseous, Gingiva, Humans, Time Factors.
Abstract
In association studies, micro-organisms can only be recognized as suspects for playing a major rôle in the development of a pathological environment, if their destructive action goes along with a marked proportional increase of their numbers or if their first detection can be related to the clinical onset of the disease. Limitations in the reproducibility of repeated samples have to be taken into account, when changes of the microbial composition of subgingival environments are to be studied, and when local clinical changes are to be related to shifts in the composition of the pertaining microbiological compartment. To study reproducibility, a total of 109 sites was sampled repeatedly with sterile paperpoints at an interval of 7 to 10 days in 24 patients suffering from periodontal disease and 12 edentulous patients wearing successful and failing osseointegrated titanium implants. Using continuous anaerobic techniques, the samples were cultured on nonselective and selective media and were studied by darkfield microscopy. Both the intertest-agreements of frequencies of detection (kappa-statistics) as well as the discrepancies of proportions of bacterial groups and selected bacterial species were determined. The standard deviation of proportional differences between first and second samples ranged between 6.4% (fusiform organisms) and 17.2% (coccoid cells) for darkfield parameters, between 4.3% (B. melaninogenicus on ETSA/Kana.) and 14.0% (B. gingivalis on ETSA/Kana.) for selected bacterial species and between 6.9% (gram-negative anaerobic cocci) and 24.0% (gram-positive facultative cocci) for bacterial groups classified according to gram stain characteristics and atmospheric growth conditions.(ABSTRACT TRUNCATED AT 250 WORDS)
PubMed: 2768538
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pubmed:2768538Le document en format XML
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<term>Dental Plaque (microbiology)</term>
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<term>Gram-Negative Bacteria (isolation & purification)</term>
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<term>Humans</term>
<term>Jaw, Edentulous (microbiology)</term>
<term>Microscopy (methods)</term>
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<term>Spirochaetales (isolation & purification)</term>
<term>Time Factors</term>
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<term>Bactéries à Gram négatif (isolement et purification)</term>
<term>Bactéries à Gram positif (isolement et purification)</term>
<term>Facteurs temps</term>
<term>Gencive</term>
<term>Humains</term>
<term>Maladies parodontales (microbiologie)</term>
<term>Microscopie ()</term>
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<term>Spirochaetales (isolement et purification)</term>
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<term>Bacteroides</term>
<term>Gram-Negative Bacteria</term>
<term>Gram-Positive Bacteria</term>
<term>Spirochaetales</term>
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<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Bacteroides</term>
<term>Bactéries</term>
<term>Bactéries à Gram négatif</term>
<term>Bactéries à Gram positif</term>
<term>Spirochaetales</term>
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<term>Periodontal Diseases</term>
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<term>Humans</term>
<term>Time Factors</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Bactéries</term>
<term>Facteurs temps</term>
<term>Gencive</term>
<term>Humains</term>
<term>Microscopie</term>
<term>Pose d'implant dentaire endo-osseux</term>
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<front><div type="abstract" xml:lang="en">In association studies, micro-organisms can only be recognized as suspects for playing a major rôle in the development of a pathological environment, if their destructive action goes along with a marked proportional increase of their numbers or if their first detection can be related to the clinical onset of the disease. Limitations in the reproducibility of repeated samples have to be taken into account, when changes of the microbial composition of subgingival environments are to be studied, and when local clinical changes are to be related to shifts in the composition of the pertaining microbiological compartment. To study reproducibility, a total of 109 sites was sampled repeatedly with sterile paperpoints at an interval of 7 to 10 days in 24 patients suffering from periodontal disease and 12 edentulous patients wearing successful and failing osseointegrated titanium implants. Using continuous anaerobic techniques, the samples were cultured on nonselective and selective media and were studied by darkfield microscopy. Both the intertest-agreements of frequencies of detection (kappa-statistics) as well as the discrepancies of proportions of bacterial groups and selected bacterial species were determined. The standard deviation of proportional differences between first and second samples ranged between 6.4% (fusiform organisms) and 17.2% (coccoid cells) for darkfield parameters, between 4.3% (B. melaninogenicus on ETSA/Kana.) and 14.0% (B. gingivalis on ETSA/Kana.) for selected bacterial species and between 6.9% (gram-negative anaerobic cocci) and 24.0% (gram-positive facultative cocci) for bacterial groups classified according to gram stain characteristics and atmospheric growth conditions.(ABSTRACT TRUNCATED AT 250 WORDS)</div>
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<Title>Journal of clinical periodontology</Title>
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<Abstract><AbstractText>In association studies, micro-organisms can only be recognized as suspects for playing a major rôle in the development of a pathological environment, if their destructive action goes along with a marked proportional increase of their numbers or if their first detection can be related to the clinical onset of the disease. Limitations in the reproducibility of repeated samples have to be taken into account, when changes of the microbial composition of subgingival environments are to be studied, and when local clinical changes are to be related to shifts in the composition of the pertaining microbiological compartment. To study reproducibility, a total of 109 sites was sampled repeatedly with sterile paperpoints at an interval of 7 to 10 days in 24 patients suffering from periodontal disease and 12 edentulous patients wearing successful and failing osseointegrated titanium implants. Using continuous anaerobic techniques, the samples were cultured on nonselective and selective media and were studied by darkfield microscopy. Both the intertest-agreements of frequencies of detection (kappa-statistics) as well as the discrepancies of proportions of bacterial groups and selected bacterial species were determined. The standard deviation of proportional differences between first and second samples ranged between 6.4% (fusiform organisms) and 17.2% (coccoid cells) for darkfield parameters, between 4.3% (B. melaninogenicus on ETSA/Kana.) and 14.0% (B. gingivalis on ETSA/Kana.) for selected bacterial species and between 6.9% (gram-negative anaerobic cocci) and 24.0% (gram-positive facultative cocci) for bacterial groups classified according to gram stain characteristics and atmospheric growth conditions.(ABSTRACT TRUNCATED AT 250 WORDS)</AbstractText>
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