Cortical Bone Morphological and Trabecular Bone Microarchitectural Changes in the Mandible and Femoral Neck of Ovariectomized Rats
Identifieur interne : 006657 ( Ncbi/Merge ); précédent : 006656; suivant : 006658Cortical Bone Morphological and Trabecular Bone Microarchitectural Changes in the Mandible and Femoral Neck of Ovariectomized Rats
Auteurs : Pei-Yu Hsu [Taïwan] ; Ming-Tzu Tsai [République populaire de Chine] ; Shun-Ping Wang [Taïwan] ; Ying-Ju Chen [Taïwan] ; Jay Wu [République populaire de Chine] ; Jui-Ting Hsu [Taïwan]Source :
- PLoS ONE [ 1932-6203 ] ; 2016.
Abstract
This study used microcomputed tomography (micro-CT) to evaluate the effects of ovariectomy on the trabecular bone microarchitecture and cortical bone morphology in the femoral neck and mandible of female rats.
Twelve female Wister rats were divided into two groups: the control and ovariectomized groups. The rats in the ovariectomized group received ovariectomy at 8 weeks of age; all the rats were sacrificed at 20 weeks of age, and their mandibles and femurs were removed and scanned using micro-CT. Four microstructural trabecular bone parameters were measured for the region below the first mandibular molar and the femoral neck region: bone volume fraction (BV/TV), trabecular thickness (TbTh), trabecular separation (TbSp), and trabecular number (TbN). In addition, four cortical bone parameters were measured for the femoral neck region: total cross-sectional area (TtAr), cortical area (CtAr), cortical bone area fraction (CtAr/TtAr), and cortical thickness (CtTh). The CtTh at the masseteric ridge was used to assess the cortical bone morphology in the mandible. The trabecular bone microarchitecture and cortical bone morphology in the femoral necks and mandibles of the control group were compared with those of the ovariectomized group. Furthermore, Spearman’s correlation (
Regarding the trabecular bone microarchitectural parameters, the BV/TV of the trabecular bone microarchitecture in the femoral necks of the control group (61.199±11.288%, median ± interquartile range) was significantly greater than that of the ovariectomized group (40.329±5.153%). Similarly, the BV/TV of the trabecular bone microarchitecture in the mandibles of the control group (51.704±6.253%) was significantly greater than that of the ovariectomized group (38.486±9.111%). Furthermore, the TbSp of the femoral necks in the ovariectomized group (0.185±0.066 mm) was significantly greater than that in the control group (0.130±0.026mm). Similarly, the TbSp of the mandibles in the ovariectomized group (0.322±0.047mm) was significantly greater than that in the control group (0.285±0.041mm). However, the TbTh and TbN trends for the mandibles and femoral necks were inconsistent between the control and ovariectomized groups. Regarding the cortical bone morphology parameters, the TtAr of the femoral necks in the ovariectomized group was significantly smaller than that in the control group. There was no significant difference in the TtAr, CtAr, or CtTh of the femoral necks between the control and ovariectomized groups, and no significant difference in the CtTh of the mandibles between the control and ovariectomized groups. Moreover, the BV/TV and TbSp of the mandibles were highly correlated with those of the femurs (
After the rats were ovariectomized, osteoporosis of the trabecular bone microarchitecture occurred in their femurs and mandibles; however, ovariectomy did not influence the cortical bone morphology. In addition, the parametric values of the trabecular bone microarchitecture in the femoral necks were highly correlated with those of the trabecular bone microarchitecture in the mandibles.
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DOI: 10.1371/journal.pone.0154367
PubMed: 27127909
PubMed Central: 4851407
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Taichung, 413, Taiwan</addr-line></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Bioinformatics and Medical Engineering, Asia University. 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Taichung, 413, Taiwan</addr-line></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Bioinformatics and Medical Engineering, Asia University. Taichung, 413</wicri:regionArea><wicri:noRegion>413</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="sec001"><title>Objective</title><p>This study used microcomputed tomography (micro-CT) to evaluate the effects of ovariectomy on the trabecular bone microarchitecture and cortical bone morphology in the femoral neck and mandible of female rats.</p></sec><sec id="sec002"><title>Materials and Methods</title><p>Twelve female Wister rats were divided into two groups: the control and ovariectomized groups. The rats in the ovariectomized group received ovariectomy at 8 weeks of age; all the rats were sacrificed at 20 weeks of age, and their mandibles and femurs were removed and scanned using micro-CT. Four microstructural trabecular bone parameters were measured for the region below the first mandibular molar and the femoral neck region: bone volume fraction (BV/TV), trabecular thickness (TbTh), trabecular separation (TbSp), and trabecular number (TbN). In addition, four cortical bone parameters were measured for the femoral neck region: total cross-sectional area (TtAr), cortical area (CtAr), cortical bone area fraction (CtAr/TtAr), and cortical thickness (CtTh). The CtTh at the masseteric ridge was used to assess the cortical bone morphology in the mandible. The trabecular bone microarchitecture and cortical bone morphology in the femoral necks and mandibles of the control group were compared with those of the ovariectomized group. Furthermore, Spearman’s correlation (<italic>r</italic><sub><italic>s</italic></sub>) was conducted to analyze the correlation between the osteoporosis conditions of the mandible and femoral neck.</p></sec><sec id="sec003"><title>Results</title><p>Regarding the trabecular bone microarchitectural parameters, the BV/TV of the trabecular bone microarchitecture in the femoral necks of the control group (61.199±11.288%, median ± interquartile range) was significantly greater than that of the ovariectomized group (40.329±5.153%). Similarly, the BV/TV of the trabecular bone microarchitecture in the mandibles of the control group (51.704±6.253%) was significantly greater than that of the ovariectomized group (38.486±9.111%). Furthermore, the TbSp of the femoral necks in the ovariectomized group (0.185±0.066 mm) was significantly greater than that in the control group (0.130±0.026mm). Similarly, the TbSp of the mandibles in the ovariectomized group (0.322±0.047mm) was significantly greater than that in the control group (0.285±0.041mm). However, the TbTh and TbN trends for the mandibles and femoral necks were inconsistent between the control and ovariectomized groups. Regarding the cortical bone morphology parameters, the TtAr of the femoral necks in the ovariectomized group was significantly smaller than that in the control group. There was no significant difference in the TtAr, CtAr, or CtTh of the femoral necks between the control and ovariectomized groups, and no significant difference in the CtTh of the mandibles between the control and ovariectomized groups. Moreover, the BV/TV and TbSp of the mandibles were highly correlated with those of the femurs (<italic>r</italic><sub><italic>s</italic></sub> = 0.874 and <italic>r</italic><sub><italic>s</italic></sub> = 0.755 for BV/TV and TbSp, respectively). Nevertheless, the TbTh, TbN, and CtTh of the mandibles were not correlated with those of the femoral necks.</p></sec><sec id="sec004"><title>Conclusion</title><p>After the rats were ovariectomized, osteoporosis of the trabecular bone microarchitecture occurred in their femurs and mandibles; however, ovariectomy did not influence the cortical bone morphology. In addition, the parametric values of the trabecular bone microarchitecture in the femoral necks were highly correlated with those of the trabecular bone microarchitecture in the mandibles.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Piscitelli, P" uniqKey="Piscitelli P">P Piscitelli</name></author><author><name sortKey="Iolascon, G" uniqKey="Iolascon G">G Iolascon</name></author><author><name sortKey="Gimigliano, F" uniqKey="Gimigliano F">F Gimigliano</name></author><author><name sortKey="Gimigliano, A" uniqKey="Gimigliano A">A Gimigliano</name></author><author><name sortKey="Marinelli, A" uniqKey="Marinelli A">A Marinelli</name></author><author><name sortKey="Di Nuzzo, R" uniqKey="Di Nuzzo R">R Di Nuzzo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Samelson, Ej" uniqKey="Samelson E">EJ Samelson</name></author><author><name sortKey="Hannan, Mt" uniqKey="Hannan M">MT 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27127909</article-id><article-id pub-id-type="pmc">4851407</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0154367</article-id><article-id pub-id-type="publisher-id">PONE-D-16-02679</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Surgical and Invasive Medical Procedures</subject><subj-group><subject>Reproductive System Procedures</subject><subj-group><subject>Ovariectomy</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Surgical and Invasive Medical Procedures</subject><subj-group><subject>Surgical Excision</subject><subj-group><subject>Ovariectomy</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Rheumatology</subject><subj-group><subject>Connective Tissue Diseases</subject><subj-group><subject>Osteoporosis</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Digestive System</subject><subj-group><subject>Mouth</subject><subj-group><subject>Mandible</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Digestive System</subject><subj-group><subject>Mouth</subject><subj-group><subject>Mandible</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Musculoskeletal System</subject><subj-group><subject>Skeleton</subject><subj-group><subject>Femur</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Musculoskeletal System</subject><subj-group><subject>Skeleton</subject><subj-group><subject>Femur</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Diagnostic Medicine</subject><subj-group><subject>Diagnostic Radiology</subject><subj-group><subject>Bone Imaging</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Imaging Techniques</subject><subj-group><subject>Diagnostic Radiology</subject><subj-group><subject>Bone Imaging</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Radiology and Imaging</subject><subj-group><subject>Diagnostic Radiology</subject><subj-group><subject>Bone Imaging</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Critical Care and Emergency Medicine</subject><subj-group><subject>Trauma Medicine</subject><subj-group><subject>Traumatic Injury</subject><subj-group><subject>Bone Fracture</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Musculoskeletal System</subject><subj-group><subject>Pelvis</subject><subj-group><subject>Hip</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Musculoskeletal System</subject><subj-group><subject>Pelvis</subject><subj-group><subject>Hip</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Bone</subject><subj-group><subject>Bone Density</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Bone</subject><subj-group><subject>Bone Density</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Biological Tissue</subject><subj-group><subject>Connective Tissue</subject><subj-group><subject>Bone</subject><subj-group><subject>Bone Density</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Medicine and Health Sciences</subject><subj-group><subject>Anatomy</subject><subj-group><subject>Biological Tissue</subject><subj-group><subject>Connective Tissue</subject><subj-group><subject>Bone</subject><subj-group><subject>Bone Density</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Cortical Bone Morphological and Trabecular Bone Microarchitectural Changes in the Mandible and Femoral Neck of Ovariectomized Rats</article-title><alt-title alt-title-type="running-head">Bone Changes in the Mandible and Femoral Neck after Ovariectomy</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Hsu</surname><given-names>Pei-Yu</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Tsai</surname><given-names>Ming-Tzu</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Shun-Ping</given-names></name><xref ref-type="aff" rid="aff003"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Ying-Ju</given-names></name><xref ref-type="aff" rid="aff004"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Wu</surname><given-names>Jay</given-names></name><xref ref-type="aff" rid="aff005"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Hsu</surname><given-names>Jui-Ting</given-names></name><xref ref-type="aff" rid="aff006"><sup>6</sup></xref><xref ref-type="aff" rid="aff007"><sup>7</sup></xref><xref ref-type="corresp" rid="cor001">*</xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung, 404, Taiwan</addr-line></aff><aff id="aff002"><label>2</label><addr-line>Department of Biomedical Engineering, Hungkuang University, Taichung, Taiwan, 433, ROC</addr-line></aff><aff id="aff003"><label>3</label><addr-line>Department of Orthopaedics, Taichung Veterans General Hospital, Taichung, 407, Taiwan</addr-line></aff><aff id="aff004"><label>4</label><addr-line>Department of Food and Nutrition, Providence University, Taichung, 433, Taiwan</addr-line></aff><aff id="aff005"><label>5</label><addr-line>Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan, 112, ROC</addr-line></aff><aff id="aff006"><label>6</label><addr-line>School of Dentistry, College of Medicine, China Medical University, Taichung, 404, Taiwan</addr-line></aff><aff id="aff007"><label>7</label><addr-line>Department of Bioinformatics and Medical Engineering, Asia University. Taichung, 413, Taiwan</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Chen</surname><given-names>Jin-Ran</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>UAMS, UNITED STATES</addr-line></aff><author-notes><fn fn-type="conflict" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: PYH SPW YJC JTH. Performed the experiments: PYH MTT SPW YJC JTH. Analyzed the data: PYH JW JTH. Contributed reagents/materials/analysis tools: PYH MTT SPW YJC JW JTH. Wrote the paper: PYH JTH.</p></fn><corresp id="cor001">* E-mail: <email>jthsu@mail.cmu.edu.tw</email>; <email>richard@ms32.url.com.tw</email></corresp></author-notes><pub-date pub-type="epub"><day>29</day><month>4</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>11</volume><issue>4</issue><elocation-id>e0154367</elocation-id><history><date date-type="received"><day>20</day><month>1</month><year>2016</year></date><date date-type="accepted"><day>12</day><month>4</month><year>2016</year></date></history><permissions><copyright-statement>© 2016 Hsu et al</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Hsu et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><self-uri content-type="pdf" xlink:href="pone.0154367.pdf"></self-uri><abstract><sec id="sec001"><title>Objective</title><p>This study used microcomputed tomography (micro-CT) to evaluate the effects of ovariectomy on the trabecular bone microarchitecture and cortical bone morphology in the femoral neck and mandible of female rats.</p></sec><sec id="sec002"><title>Materials and Methods</title><p>Twelve female Wister rats were divided into two groups: the control and ovariectomized groups. The rats in the ovariectomized group received ovariectomy at 8 weeks of age; all the rats were sacrificed at 20 weeks of age, and their mandibles and femurs were removed and scanned using micro-CT. Four microstructural trabecular bone parameters were measured for the region below the first mandibular molar and the femoral neck region: bone volume fraction (BV/TV), trabecular thickness (TbTh), trabecular separation (TbSp), and trabecular number (TbN). In addition, four cortical bone parameters were measured for the femoral neck region: total cross-sectional area (TtAr), cortical area (CtAr), cortical bone area fraction (CtAr/TtAr), and cortical thickness (CtTh). The CtTh at the masseteric ridge was used to assess the cortical bone morphology in the mandible. The trabecular bone microarchitecture and cortical bone morphology in the femoral necks and mandibles of the control group were compared with those of the ovariectomized group. Furthermore, Spearman’s correlation (<italic>r</italic><sub><italic>s</italic></sub>) was conducted to analyze the correlation between the osteoporosis conditions of the mandible and femoral neck.</p></sec><sec id="sec003"><title>Results</title><p>Regarding the trabecular bone microarchitectural parameters, the BV/TV of the trabecular bone microarchitecture in the femoral necks of the control group (61.199±11.288%, median ± interquartile range) was significantly greater than that of the ovariectomized group (40.329±5.153%). Similarly, the BV/TV of the trabecular bone microarchitecture in the mandibles of the control group (51.704±6.253%) was significantly greater than that of the ovariectomized group (38.486±9.111%). Furthermore, the TbSp of the femoral necks in the ovariectomized group (0.185±0.066 mm) was significantly greater than that in the control group (0.130±0.026mm). Similarly, the TbSp of the mandibles in the ovariectomized group (0.322±0.047mm) was significantly greater than that in the control group (0.285±0.041mm). However, the TbTh and TbN trends for the mandibles and femoral necks were inconsistent between the control and ovariectomized groups. Regarding the cortical bone morphology parameters, the TtAr of the femoral necks in the ovariectomized group was significantly smaller than that in the control group. There was no significant difference in the TtAr, CtAr, or CtTh of the femoral necks between the control and ovariectomized groups, and no significant difference in the CtTh of the mandibles between the control and ovariectomized groups. Moreover, the BV/TV and TbSp of the mandibles were highly correlated with those of the femurs (<italic>r</italic><sub><italic>s</italic></sub> = 0.874 and <italic>r</italic><sub><italic>s</italic></sub> = 0.755 for BV/TV and TbSp, respectively). Nevertheless, the TbTh, TbN, and CtTh of the mandibles were not correlated with those of the femoral necks.</p></sec><sec id="sec004"><title>Conclusion</title><p>After the rats were ovariectomized, osteoporosis of the trabecular bone microarchitecture occurred in their femurs and mandibles; however, ovariectomy did not influence the cortical bone morphology. In addition, the parametric values of the trabecular bone microarchitecture in the femoral necks were highly correlated with those of the trabecular bone microarchitecture in the mandibles.</p></sec></abstract><funding-group><award-group id="award001"><funding-source><institution-wrap><institution-id institution-id-type="funder-id">http://dx.doi.org/10.13039/501100004663</institution-id><institution>Ministry of Science and Technology, Taiwan</institution></institution-wrap></funding-source><award-id>104-2815-C-039-038-B</award-id><principal-award-recipient><name><surname>Hsu</surname><given-names>Pei-Yu</given-names></name></principal-award-recipient></award-group><funding-statement>This study was supported by the Ministry of Science and Technology, Taiwan (MOST 104-2815-C-039-038-B).</funding-statement></funding-group><counts><fig-count count="3"></fig-count><table-count count="4"></table-count><page-count count="16"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All relevant data are within the paper and its Supporting Information files.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All relevant data are within the paper and its Supporting Information files.</p></notes></front></pmc><affiliations><list><country><li>République populaire de Chine</li><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Hsu, Pei Yu" sort="Hsu, Pei Yu" uniqKey="Hsu P" first="Pei-Yu" last="Hsu">Pei-Yu Hsu</name></noRegion><name sortKey="Chen, Ying Ju" sort="Chen, Ying Ju" uniqKey="Chen Y" first="Ying-Ju" last="Chen">Ying-Ju Chen</name><name sortKey="Hsu, Jui Ting" sort="Hsu, Jui Ting" uniqKey="Hsu J" first="Jui-Ting" last="Hsu">Jui-Ting Hsu</name><name sortKey="Hsu, Jui Ting" sort="Hsu, Jui Ting" uniqKey="Hsu J" first="Jui-Ting" last="Hsu">Jui-Ting Hsu</name><name sortKey="Wang, Shun Ping" sort="Wang, Shun Ping" uniqKey="Wang S" first="Shun-Ping" last="Wang">Shun-Ping Wang</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Tsai, Ming Tzu" sort="Tsai, Ming Tzu" uniqKey="Tsai M" first="Ming-Tzu" last="Tsai">Ming-Tzu Tsai</name></noRegion><name sortKey="Wu, Jay" sort="Wu, Jay" uniqKey="Wu J" first="Jay" last="Wu">Jay Wu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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