Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration
Identifieur interne : 006625 ( Ncbi/Merge ); précédent : 006624; suivant : 006626Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration
Auteurs : Chae-Kyung Yoo [Corée du Sud] ; Jae-Yun Jeon [Corée du Sud] ; You-Jin Kim [Corée du Sud] ; Seong-Gon Kim [Corée du Sud] ; Kyung-Gyun Hwang [Corée du Sud]Source :
- Maxillofacial Plastic and Reconstructive Surgery [ 2288-8101 ] ; 2016.
Abstract
The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR).
Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cells cultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, the attachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy (SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation was quantified using 4′,6-diamidino-2-phenylindole (DAPI) fluorescence staining.
Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-day observation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (
These results suggest that silk fibroin is a biocompatible material that could be used as a suitable alternative barrier membrane for GBR.
Url:
DOI: 10.1186/s40902-016-0062-4
PubMed: 27073799
PubMed Central: 4819730
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<series><title level="j">Maxillofacial Plastic and Reconstructive Surgery</title>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR).</p>
</sec>
<sec><title>Methods</title>
<p>Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cells cultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, the attachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy (SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation was quantified using 4′,6-diamidino-2-phenylindole (DAPI) fluorescence staining.</p>
</sec>
<sec><title>Results</title>
<p>Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-day observation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (<italic>P</italic>
< 0.05); however, at respective time points, cell viability was not significantly different between the two groups (<italic>P</italic>
> 0.05). In contrast, cell proliferation was significantly higher in the SF membrane group than in the control group at 7 days (<italic>P</italic>
< 0.05).</p>
</sec>
<sec><title>Conclusions</title>
<p>These results suggest that silk fibroin is a biocompatible material that could be used as a suitable alternative barrier membrane for GBR.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Mcallister, Bs" uniqKey="Mcallister B">BS McAllister</name>
</author>
<author><name sortKey="Haghighat, K" uniqKey="Haghighat K">K Haghighat</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Caplanis, N" uniqKey="Caplanis N">N Caplanis</name>
</author>
<author><name sortKey="Sigurdsson, Tj" uniqKey="Sigurdsson T">TJ Sigurdsson</name>
</author>
<author><name sortKey="Rohrer, Md" uniqKey="Rohrer M">MD Rohrer</name>
</author>
<author><name sortKey="Wikesjo, U" uniqKey="Wikesjo U">U Wikesjö</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Massaro, D" uniqKey="Massaro D">D Massaro</name>
</author>
<author><name sortKey="Massaro, Gd" uniqKey="Massaro G">GD Massaro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hurley, La" uniqKey="Hurley L">LA Hurley</name>
</author>
<author><name sortKey="Stinchfield, Fe" uniqKey="Stinchfield F">FE Stinchfield</name>
</author>
<author><name sortKey="Bassett, A" uniqKey="Bassett A">A Bassett</name>
</author>
<author><name sortKey="Lyon, Wh" uniqKey="Lyon W">WH Lyon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Melcher, A" uniqKey="Melcher A">A Melcher</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kansy, M" uniqKey="Kansy M">M Kansy</name>
</author>
<author><name sortKey="Senner, F" uniqKey="Senner F">F Senner</name>
</author>
<author><name sortKey="Gubernator, K" uniqKey="Gubernator K">K Gubernator</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yuan, J" uniqKey="Yuan J">J Yuan</name>
</author>
<author><name sortKey="Liu, X" uniqKey="Liu X">X Liu</name>
</author>
<author><name sortKey="Akbulut, O" uniqKey="Akbulut O">O Akbulut</name>
</author>
<author><name sortKey="Hu, J" uniqKey="Hu J">J Hu</name>
</author>
<author><name sortKey="Suib, Sl" uniqKey="Suib S">SL Suib</name>
</author>
<author><name sortKey="Kong, J" uniqKey="Kong J">J Kong</name>
</author>
<author><name sortKey="Stellacci, F" uniqKey="Stellacci F">F Stellacci</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Griffith, Lg" uniqKey="Griffith L">LG Griffith</name>
</author>
<author><name sortKey="Naughton, G" uniqKey="Naughton G">G Naughton</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Zitzmann, Nu" uniqKey="Zitzmann N">NU Zitzmann</name>
</author>
<author><name sortKey="Naef, R" uniqKey="Naef R">R Naef</name>
</author>
<author><name sortKey="Sch Rer, P" uniqKey="Sch Rer P">P Schärer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fujihara, K" uniqKey="Fujihara K">K Fujihara</name>
</author>
<author><name sortKey="Kotaki, M" uniqKey="Kotaki M">M Kotaki</name>
</author>
<author><name sortKey="Ramakrishna, S" uniqKey="Ramakrishna S">S Ramakrishna</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Roccuzzo, M" uniqKey="Roccuzzo M">M Roccuzzo</name>
</author>
<author><name sortKey="Bunino, M" uniqKey="Bunino M">M Bunino</name>
</author>
<author><name sortKey="Needleman, I" uniqKey="Needleman I">I Needleman</name>
</author>
<author><name sortKey="Sanz, M" uniqKey="Sanz M">M Sanz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Simion, M" uniqKey="Simion M">M Simion</name>
</author>
<author><name sortKey="Scarano, A" uniqKey="Scarano A">A Scarano</name>
</author>
<author><name sortKey="Gionso, L" uniqKey="Gionso L">L Gionso</name>
</author>
<author><name sortKey="Piattelli, A" uniqKey="Piattelli A">A Piattelli</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Xia, Q" uniqKey="Xia Q">Q Xia</name>
</author>
<author><name sortKey="Zhou, Z" uniqKey="Zhou Z">Z Zhou</name>
</author>
<author><name sortKey="Lu, C" uniqKey="Lu C">C Lu</name>
</author>
<author><name sortKey="Cheng, D" uniqKey="Cheng D">D Cheng</name>
</author>
<author><name sortKey="Dai, F" uniqKey="Dai F">F Dai</name>
</author>
<author><name sortKey="Li, B" uniqKey="Li B">B Li</name>
</author>
<author><name sortKey="Zhao, P" uniqKey="Zhao P">P Zhao</name>
</author>
<author><name sortKey="Zha, X" uniqKey="Zha X">X Zha</name>
</author>
<author><name sortKey="Cheng, T" uniqKey="Cheng T">T Cheng</name>
</author>
<author><name sortKey="Chai, C" uniqKey="Chai C">C Chai</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hutmacher, Dw" uniqKey="Hutmacher D">DW Hutmacher</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lutolf, M" uniqKey="Lutolf M">M Lutolf</name>
</author>
<author><name sortKey="Hubbell, J" uniqKey="Hubbell J">J Hubbell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bunyaratavej, P" uniqKey="Bunyaratavej P">P Bunyaratavej</name>
</author>
<author><name sortKey="Wang, H L" uniqKey="Wang H">H-L Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kim, K H" uniqKey="Kim K">K-H Kim</name>
</author>
<author><name sortKey="Jeong, L" uniqKey="Jeong L">L Jeong</name>
</author>
<author><name sortKey="Park, H N" uniqKey="Park H">H-N Park</name>
</author>
<author><name sortKey="Shin, S Y" uniqKey="Shin S">S-Y Shin</name>
</author>
<author><name sortKey="Park, W H" uniqKey="Park W">W-H Park</name>
</author>
<author><name sortKey="Lee, S C" uniqKey="Lee S">S-C Lee</name>
</author>
<author><name sortKey="Kim, T I" uniqKey="Kim T">T-I Kim</name>
</author>
<author><name sortKey="Park, Y J" uniqKey="Park Y">Y-J Park</name>
</author>
<author><name sortKey="Seol, Y J" uniqKey="Seol Y">Y-J Seol</name>
</author>
<author><name sortKey="Lee, Y M" uniqKey="Lee Y">Y-M Lee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kieswetter, K" uniqKey="Kieswetter K">K Kieswetter</name>
</author>
<author><name sortKey="Schwartz, Z" uniqKey="Schwartz Z">Z Schwartz</name>
</author>
<author><name sortKey="Hummert, T" uniqKey="Hummert T">T Hummert</name>
</author>
<author><name sortKey="Cochran, D" uniqKey="Cochran D">D Cochran</name>
</author>
<author><name sortKey="Simpson, J" uniqKey="Simpson J">J Simpson</name>
</author>
<author><name sortKey="Dean, D" uniqKey="Dean D">D Dean</name>
</author>
<author><name sortKey="Boyan, B" uniqKey="Boyan B">B Boyan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sui, G" uniqKey="Sui G">G Sui</name>
</author>
<author><name sortKey="Yang, X" uniqKey="Yang X">X Yang</name>
</author>
<author><name sortKey="Mei, F" uniqKey="Mei F">F Mei</name>
</author>
<author><name sortKey="Hu, X" uniqKey="Hu X">X Hu</name>
</author>
<author><name sortKey="Chen, G" uniqKey="Chen G">G Chen</name>
</author>
<author><name sortKey="Deng, X" uniqKey="Deng X">X Deng</name>
</author>
<author><name sortKey="Ryu, S" uniqKey="Ryu S">S Ryu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, H C" uniqKey="Liu H">H-C Liu</name>
</author>
<author><name sortKey="Lee, I" uniqKey="Lee I">I Lee</name>
</author>
<author><name sortKey="Wang, J H" uniqKey="Wang J">J-H Wang</name>
</author>
<author><name sortKey="Yang, S H" uniqKey="Yang S">S-H Yang</name>
</author>
<author><name sortKey="Young, T H" uniqKey="Young T">T-H Young</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Unger, Re" uniqKey="Unger R">RE Unger</name>
</author>
<author><name sortKey="Sartoris, A" uniqKey="Sartoris A">A Sartoris</name>
</author>
<author><name sortKey="Peters, K" uniqKey="Peters K">K Peters</name>
</author>
<author><name sortKey="Motta, A" uniqKey="Motta A">A Motta</name>
</author>
<author><name sortKey="Migliaresi, C" uniqKey="Migliaresi C">C Migliaresi</name>
</author>
<author><name sortKey="Kunkel, M" uniqKey="Kunkel M">M Kunkel</name>
</author>
<author><name sortKey="Bulnheim, U" uniqKey="Bulnheim U">U Bulnheim</name>
</author>
<author><name sortKey="Rychly, J" uniqKey="Rychly J">J Rychly</name>
</author>
<author><name sortKey="James Kirkpatrick, C" uniqKey="James Kirkpatrick C">C James Kirkpatrick</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vepari, C" uniqKey="Vepari C">C Vepari</name>
</author>
<author><name sortKey="Kaplan, Dl" uniqKey="Kaplan D">DL Kaplan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="H Mmerle, Ch" uniqKey="H Mmerle C">CH Hämmerle</name>
</author>
<author><name sortKey="Jung, Re" uniqKey="Jung R">RE Jung</name>
</author>
<author><name sortKey="Feloutzis, A" uniqKey="Feloutzis A">A Feloutzis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Minoura, N" uniqKey="Minoura N">N Minoura</name>
</author>
<author><name sortKey="Tsukada, M" uniqKey="Tsukada M">M Tsukada</name>
</author>
<author><name sortKey="Nagura, M" uniqKey="Nagura M">M Nagura</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Berahim, Z" uniqKey="Berahim Z">Z Berahim</name>
</author>
<author><name sortKey="Moharamzadeh, K" uniqKey="Moharamzadeh K">K Moharamzadeh</name>
</author>
<author><name sortKey="Rawlinson, A" uniqKey="Rawlinson A">A Rawlinson</name>
</author>
<author><name sortKey="Jowett, Ak" uniqKey="Jowett A">AK Jowett</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Carpio, L" uniqKey="Carpio L">L Carpio</name>
</author>
<author><name sortKey="Loza, J" uniqKey="Loza J">J Loza</name>
</author>
<author><name sortKey="Lynch, S" uniqKey="Lynch S">S Lynch</name>
</author>
<author><name sortKey="Genco, R" uniqKey="Genco R">R Genco</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, Hl" uniqKey="Wang H">HL Wang</name>
</author>
<author><name sortKey="Miyauchi, M" uniqKey="Miyauchi M">M Miyauchi</name>
</author>
<author><name sortKey="Takata, T" uniqKey="Takata T">T Takata</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Song, J Y" uniqKey="Song J">J-Y Song</name>
</author>
<author><name sortKey="Kim, S G" uniqKey="Kim S">S-G Kim</name>
</author>
<author><name sortKey="Lee, J W" uniqKey="Lee J">J-W Lee</name>
</author>
<author><name sortKey="Chae, W S" uniqKey="Chae W">W-S Chae</name>
</author>
<author><name sortKey="Kweon, H" uniqKey="Kweon H">H Kweon</name>
</author>
<author><name sortKey="Jo, Y Y" uniqKey="Jo Y">Y-Y Jo</name>
</author>
<author><name sortKey="Lee, K G" uniqKey="Lee K">K-G Lee</name>
</author>
<author><name sortKey="Lee, Y C" uniqKey="Lee Y">Y-C Lee</name>
</author>
<author><name sortKey="Choi, J Y" uniqKey="Choi J">J-Y Choi</name>
</author>
<author><name sortKey="Kim, J Y" uniqKey="Kim J">J-Y Kim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cai, K" uniqKey="Cai K">K Cai</name>
</author>
<author><name sortKey="Yao, K" uniqKey="Yao K">K Yao</name>
</author>
<author><name sortKey="Lin, S" uniqKey="Lin S">S Lin</name>
</author>
<author><name sortKey="Yang, Z" uniqKey="Yang Z">Z Yang</name>
</author>
<author><name sortKey="Li, X" uniqKey="Li X">X Li</name>
</author>
<author><name sortKey="Xie, H" uniqKey="Xie H">H Xie</name>
</author>
<author><name sortKey="Qing, T" uniqKey="Qing T">T Qing</name>
</author>
<author><name sortKey="Gao, L" uniqKey="Gao L">L Gao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, W" uniqKey="Chen W">W Chen</name>
</author>
<author><name sortKey="Wang, Ww" uniqKey="Wang W">WW Wang</name>
</author>
<author><name sortKey="Shi, Xz" uniqKey="Shi X">XZ Shi</name>
</author>
<author><name sortKey="Chen, N" uniqKey="Chen N">N Chen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kundu, B" uniqKey="Kundu B">B Kundu</name>
</author>
<author><name sortKey="Rajkhowa, R" uniqKey="Rajkhowa R">R Rajkhowa</name>
</author>
<author><name sortKey="Kundu, Sc" uniqKey="Kundu S">SC Kundu</name>
</author>
<author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lee, S W" uniqKey="Lee S">S-W Lee</name>
</author>
<author><name sortKey="Kim, S G" uniqKey="Kim S">S-G Kim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jang, E S" uniqKey="Jang E">E-S Jang</name>
</author>
<author><name sortKey="Park, J W" uniqKey="Park J">J-W Park</name>
</author>
<author><name sortKey="Kweon, H" uniqKey="Kweon H">H Kweon</name>
</author>
<author><name sortKey="Lee, K G" uniqKey="Lee K">K-G Lee</name>
</author>
<author><name sortKey="Kang, S W" uniqKey="Kang S">S-W Kang</name>
</author>
<author><name sortKey="Baek, D H" uniqKey="Baek D">D-H Baek</name>
</author>
<author><name sortKey="Choi, J Y" uniqKey="Choi J">J-Y Choi</name>
</author>
<author><name sortKey="Kim, S G" uniqKey="Kim S">S-G Kim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sofia, S" uniqKey="Sofia S">S Sofia</name>
</author>
<author><name sortKey="Mccarthy, Mb" uniqKey="Mccarthy M">MB McCarthy</name>
</author>
<author><name sortKey="Gronowicz, G" uniqKey="Gronowicz G">G Gronowicz</name>
</author>
<author><name sortKey="Kaplan, Dl" uniqKey="Kaplan D">DL Kaplan</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Maxillofac Plast Reconstr Surg</journal-id>
<journal-id journal-id-type="iso-abbrev">Maxillofac Plast Reconstr Surg</journal-id>
<journal-title-group><journal-title>Maxillofacial Plastic and Reconstructive Surgery</journal-title>
</journal-title-group>
<issn pub-type="ppub">2288-8101</issn>
<issn pub-type="epub">2288-8586</issn>
<publisher><publisher-name>Springer Berlin Heidelberg</publisher-name>
<publisher-loc>Berlin/Heidelberg</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">27073799</article-id>
<article-id pub-id-type="pmc">4819730</article-id>
<article-id pub-id-type="publisher-id">62</article-id>
<article-id pub-id-type="doi">10.1186/s40902-016-0062-4</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research</subject>
</subj-group>
</article-categories>
<title-group><article-title>Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Yoo</surname>
<given-names>Chae-Kyung</given-names>
</name>
<address><email>dotol99@daum.net</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author" equal-contrib="yes"><name><surname>Jeon</surname>
<given-names>Jae-Yun</given-names>
</name>
<address><email>fellowjy@daum.net</email>
</address>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kim</surname>
<given-names>You-Jin</given-names>
</name>
<address><email>rladb97@naver.com</email>
</address>
<xref ref-type="aff" rid="Aff3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kim</surname>
<given-names>Seong-Gon</given-names>
</name>
<address><email>kimsg@gwnu.ac.kr</email>
</address>
<xref ref-type="aff" rid="Aff4">4</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Hwang</surname>
<given-names>Kyung-Gyun</given-names>
</name>
<address><phone>+82-2-2290-8676</phone>
<email>hkg@hanyang.ac.kr</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<aff id="Aff1"><label>1</label>
<institution-wrap><institution-id institution-id-type="GRID">grid.49606.3d</institution-id>
<institution-id institution-id-type="ISNI">0000000113649317</institution-id>
<institution>Department of Translational Medicine, Graduate School of Biomedical Science and Engineering,</institution>
<institution>Hanyang University,</institution>
</institution-wrap>
Seoul, South Korea</aff>
<aff id="Aff2"><label>2</label>
<institution-wrap><institution-id institution-id-type="GRID">grid.49606.3d</institution-id>
<institution-id institution-id-type="ISNI">0000000113649317</institution-id>
<institution>Department of Dentistry/Division of Oral and Maxillofacial Surgery, College of Medicine,</institution>
<institution>Hanyang University,</institution>
</institution-wrap>
222 Wangsimni-ro, Seongdong-gu, Seoul, 133-791 South Korea</aff>
<aff id="Aff3"><label>3</label>
Korean Minjok Leadership Academy, Hoengseong, South Korea</aff>
<aff id="Aff4"><label>4</label>
<institution-wrap><institution-id institution-id-type="GRID">grid.411733.3</institution-id>
<institution-id institution-id-type="ISNI">000000040532811X</institution-id>
<institution>Department of Oral and Maxillofacial Surgery, College of Dentistry,</institution>
<institution>Gangneung-Wonju National University,</institution>
</institution-wrap>
Gangneung, South Korea</aff>
</contrib-group>
<pub-date pub-type="epub"><day>30</day>
<month>3</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>30</day>
<month>3</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="collection"><month>12</month>
<year>2016</year>
</pub-date>
<volume>38</volume>
<issue>1</issue>
<elocation-id>17</elocation-id>
<history><date date-type="received"><day>21</day>
<month>1</month>
<year>2016</year>
</date>
<date date-type="accepted"><day>10</day>
<month>3</month>
<year>2016</year>
</date>
</history>
<permissions><copyright-statement>© Yoo et al. 2016</copyright-statement>
<license license-type="OpenAccess"><license-p><bold>Open Access</bold>
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.</license-p>
</license>
</permissions>
<abstract id="Abs1"><sec><title>Background</title>
<p>The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR).</p>
</sec>
<sec><title>Methods</title>
<p>Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cells cultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, the attachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy (SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation was quantified using 4′,6-diamidino-2-phenylindole (DAPI) fluorescence staining.</p>
</sec>
<sec><title>Results</title>
<p>Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-day observation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (<italic>P</italic>
< 0.05); however, at respective time points, cell viability was not significantly different between the two groups (<italic>P</italic>
> 0.05). In contrast, cell proliferation was significantly higher in the SF membrane group than in the control group at 7 days (<italic>P</italic>
< 0.05).</p>
</sec>
<sec><title>Conclusions</title>
<p>These results suggest that silk fibroin is a biocompatible material that could be used as a suitable alternative barrier membrane for GBR.</p>
</sec>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd>Bone regeneration</kwd>
<kwd>Cell adhesion</kwd>
<kwd>Cell proliferation</kwd>
<kwd>Membrane</kwd>
<kwd>Osteoblasts</kwd>
<kwd>Silk fibroin</kwd>
</kwd-group>
<funding-group><award-group><funding-source><institution>National Research Foundation of Korea (NRF)</institution>
</funding-source>
<award-id>NRF-2013R1A1A2009975</award-id>
</award-group>
<award-group><funding-source><institution>Next-Generation BioGreen 21 Program</institution>
</funding-source>
<award-id>No.PJ01121404</award-id>
</award-group>
</funding-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© The Author(s) 2016</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Corée du Sud</li>
</country>
<region><li>Région capitale de Séoul</li>
</region>
<settlement><li>Séoul</li>
</settlement>
</list>
<tree><country name="Corée du Sud"><noRegion><name sortKey="Yoo, Chae Kyung" sort="Yoo, Chae Kyung" uniqKey="Yoo C" first="Chae-Kyung" last="Yoo">Chae-Kyung Yoo</name>
</noRegion>
<name sortKey="Hwang, Kyung Gyun" sort="Hwang, Kyung Gyun" uniqKey="Hwang K" first="Kyung-Gyun" last="Hwang">Kyung-Gyun Hwang</name>
<name sortKey="Hwang, Kyung Gyun" sort="Hwang, Kyung Gyun" uniqKey="Hwang K" first="Kyung-Gyun" last="Hwang">Kyung-Gyun Hwang</name>
<name sortKey="Jeon, Jae Yun" sort="Jeon, Jae Yun" uniqKey="Jeon J" first="Jae-Yun" last="Jeon">Jae-Yun Jeon</name>
<name sortKey="Kim, Seong Gon" sort="Kim, Seong Gon" uniqKey="Kim S" first="Seong-Gon" last="Kim">Seong-Gon Kim</name>
<name sortKey="Kim, You Jin" sort="Kim, You Jin" uniqKey="Kim Y" first="You-Jin" last="Kim">You-Jin Kim</name>
</country>
</tree>
</affiliations>
</record>
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