Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats
Identifieur interne : 005785 ( Ncbi/Merge ); précédent : 005784; suivant : 005786Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats
Auteurs : Tábata De Mello Tera [Brésil] ; Rodrigo Dias Nascimento [Brésil] ; Renata Falchete Do Prado [Brésil] ; Mauro Pedrine Santamaria [Brésil] ; Maria Aparecida Neves Jardini [Brésil]Source :
- Journal of Applied Oral Science [ 1678-7757 ] ; 2014.
Abstract
The aim of this paper was to evaluate the repair of onlay autogenous bone grafts covered or not covered by an expanded polytetrafluoroethylene (e-PTFE) membrane using immunohistochemistry in rats with induced estrogen deficiency.
Eighty female rats were randomly divided into two groups: ovariectomized (OVX) and with a simulation of the surgical procedure (SHAM). Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM). Animals were euthanized on days 0, 7, 21, 45, and 60. The specimens were subjected to immunohistochemistry for bone sialoprotein (BSP), osteonectin (ONC), and osteocalcin (OCC).
All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense. At the 45-day, the bone graft showed direct bonding to the recipient bed in all specimens. The ONC and OCC showed more expressed in granulation tissue, in the membrane groups, independently of estrogen deficiency.
The expression of bone forming markers was not negatively influenced by estrogen deficiency. However, the markers could be influenced by the presence of the e-PTFE membrane.
Url:
DOI: 10.1590/1678-775720140190
PubMed: 25591022
PubMed Central: 4307769
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PMC:4307769Le document en format XML
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<front><div type="abstract" xml:lang="en"><sec><title>Objective</title>
<p> The aim of this paper was to evaluate the repair of onlay autogenous bone grafts covered or not covered by an expanded polytetrafluoroethylene (e-PTFE) membrane using immunohistochemistry in rats with induced estrogen deficiency.</p>
</sec>
<sec><title>Material and Methods</title>
<p> Eighty female rats were randomly divided into two groups: ovariectomized (OVX) and with a simulation of the surgical procedure (SHAM). Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM). Animals were euthanized on days 0, 7, 21, 45, and 60. The specimens were subjected to immunohistochemistry for bone sialoprotein (BSP), osteonectin (ONC), and osteocalcin (OCC).</p>
</sec>
<sec><title>Results</title>
<p> All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense. At the 45-day, the bone graft showed direct bonding to the recipient bed in all specimens. The ONC and OCC showed more expressed in granulation tissue, in the membrane groups, independently of estrogen deficiency.</p>
</sec>
<sec><title>Conclusions</title>
<p> The expression of bone forming markers was not negatively influenced by estrogen deficiency. However, the markers could be influenced by the presence of the e-PTFE membrane.</p>
</sec>
</div>
</front>
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<biblStruct><analytic><author><name sortKey="Rachner, Td" uniqKey="Rachner T">TD Rachner</name>
</author>
<author><name sortKey="Khosla, S" uniqKey="Khosla S">S Khosla</name>
</author>
<author><name sortKey="Hofbauer, Lc" uniqKey="Hofbauer L">LC Hofbauer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Retzepi, M" uniqKey="Retzepi M">M Retzepi</name>
</author>
<author><name sortKey="Donos, N" uniqKey="Donos N">N Donos</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Roach, Hi" uniqKey="Roach H">HI Roach</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shoji, K" uniqKey="Shoji K">K Shoji</name>
</author>
<author><name sortKey="Elsubeihi, Es" uniqKey="Elsubeihi E">ES Elsubeihi</name>
</author>
<author><name sortKey="Heersche, Jn" uniqKey="Heersche J">JN Heersche</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Taguchi, Y" uniqKey="Taguchi Y">Y Taguchi</name>
</author>
<author><name sortKey="Amizuka, N" uniqKey="Amizuka N">N Amizuka</name>
</author>
<author><name sortKey="Nakadate, M" uniqKey="Nakadate M">M Nakadate</name>
</author>
<author><name sortKey="Ohnishi, H" uniqKey="Ohnishi H">H Ohnishi</name>
</author>
<author><name sortKey="Fujii, N" uniqKey="Fujii N">N Fujii</name>
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<author><name sortKey="Oda, K" uniqKey="Oda K">K Oda</name>
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<biblStruct><analytic><author><name sortKey="Wronski, Tj" uniqKey="Wronski T">TJ Wronski</name>
</author>
<author><name sortKey="Cintr N, M" uniqKey="Cintr N M">M Cintrón</name>
</author>
<author><name sortKey="Dann, Lm" uniqKey="Dann L">LM Dann</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Appl Oral Sci</journal-id>
<journal-id journal-id-type="iso-abbrev">J Appl Oral Sci</journal-id>
<journal-title-group><journal-title>Journal of Applied Oral Science</journal-title>
</journal-title-group>
<issn pub-type="ppub">1678-7757</issn>
<issn pub-type="epub">1678-7765</issn>
<publisher><publisher-name>Faculdade de Odontologia de Bauru da Universidade de São Paulo</publisher-name>
<publisher-loc>Bauru</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">25591022</article-id>
<article-id pub-id-type="pmc">4307769</article-id>
<article-id pub-id-type="publisher-id">1678-775720140190</article-id>
<article-id pub-id-type="doi">10.1590/1678-775720140190</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>TERA</surname>
<given-names>Tábata de Mello</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>NASCIMENTO</surname>
<given-names>Rodrigo Dias</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>do PRADO</surname>
<given-names>Renata Falchete</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>SANTAMARIA</surname>
<given-names>Mauro Pedrine</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>JARDINI</surname>
<given-names>Maria Aparecida Neves</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>1</label>
- Department of Bioscience and Oral Diagnosis, Instituto de Ciência e Tecnologia − ICT, Univ. Estadual Paulista − UNESP, São José dos Campos, SP, Brazil.</aff>
<aff id="aff2"><label>2</label>
- Department of Diagnosis and Surgery, Instituto de Ciência e Tecnologia − ICT, Univ. Estadual Paulista − UNESP, São José dos Campos, SP, Brazil.</aff>
<author-notes><corresp><bold>Corresponding address:</bold>
Maria Aparecida Neves Jardini - Department of Diagnosis and Surgery - Av. Eng. Francisco José Longo, 777 - Jd. São Dimas - São José dos Campos - 12245-000 - SP - Brazil - Phone: (55 12) 3947-9043 - Fax: (55 12) 3947-9010 - e-mail: <email>jardini@fosjc.unesp.br</email>
</corresp>
</author-notes>
<pub-date pub-type="epub-ppub"><season>Nov-Dec</season>
<year>2014</year>
</pub-date>
<pmc-comment>Fake ppub date generated by PMC from publisher
pub-date/@pub-type='epub-ppub' </pmc-comment>
<pub-date pub-type="ppub"><season>Nov-Dec</season>
<year>2014</year>
</pub-date>
<volume>22</volume>
<issue>6</issue>
<fpage>541</fpage>
<lpage>553</lpage>
<history><date date-type="received"><day>12</day>
<month>5</month>
<year>2014</year>
</date>
<date date-type="rev-recd"><day>11</day>
<month>8</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>3</day>
<month>9</month>
<year>2014</year>
</date>
</history>
<permissions><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
</license>
</permissions>
<abstract><sec><title>Objective</title>
<p> The aim of this paper was to evaluate the repair of onlay autogenous bone grafts covered or not covered by an expanded polytetrafluoroethylene (e-PTFE) membrane using immunohistochemistry in rats with induced estrogen deficiency.</p>
</sec>
<sec><title>Material and Methods</title>
<p> Eighty female rats were randomly divided into two groups: ovariectomized (OVX) and with a simulation of the surgical procedure (SHAM). Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM). Animals were euthanized on days 0, 7, 21, 45, and 60. The specimens were subjected to immunohistochemistry for bone sialoprotein (BSP), osteonectin (ONC), and osteocalcin (OCC).</p>
</sec>
<sec><title>Results</title>
<p> All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense. At the 45-day, the bone graft showed direct bonding to the recipient bed in all specimens. The ONC and OCC showed more expressed in granulation tissue, in the membrane groups, independently of estrogen deficiency.</p>
</sec>
<sec><title>Conclusions</title>
<p> The expression of bone forming markers was not negatively influenced by estrogen deficiency. However, the markers could be influenced by the presence of the e-PTFE membrane.</p>
</sec>
</abstract>
<kwd-group><kwd>Estrogen</kwd>
<kwd>Bone regeneration</kwd>
<kwd>Osteocalcin</kwd>
<kwd>Osteonectin</kwd>
<kwd>Sialoprotein</kwd>
</kwd-group>
<counts><fig-count count="11"></fig-count>
<ref-count count="30"></ref-count>
<page-count count="13"></page-count>
</counts>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Brésil</li>
</country>
<region><li>État de São Paulo</li>
</region>
</list>
<tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Tera, Tabata De Mello" sort="Tera, Tabata De Mello" uniqKey="Tera T" first="Tábata De Mello" last="Tera">Tábata De Mello Tera</name>
</region>
<name sortKey="Do Prado, Renata Falchete" sort="Do Prado, Renata Falchete" uniqKey="Do Prado R" first="Renata Falchete" last="Do Prado">Renata Falchete Do Prado</name>
<name sortKey="Jardini, Maria Aparecida Neves" sort="Jardini, Maria Aparecida Neves" uniqKey="Jardini M" first="Maria Aparecida Neves" last="Jardini">Maria Aparecida Neves Jardini</name>
<name sortKey="Nascimento, Rodrigo Dias" sort="Nascimento, Rodrigo Dias" uniqKey="Nascimento R" first="Rodrigo Dias" last="Nascimento">Rodrigo Dias Nascimento</name>
<name sortKey="Santamaria, Mauro Pedrine" sort="Santamaria, Mauro Pedrine" uniqKey="Santamaria M" first="Mauro Pedrine" last="Santamaria">Mauro Pedrine Santamaria</name>
</country>
</tree>
</affiliations>
</record>
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