Focal adhesion linker proteins expression of fibroblast related to adhesion in response to different transmucosal abutment surfaces
Identifieur interne : 004996 ( Ncbi/Merge ); précédent : 004995; suivant : 004997Focal adhesion linker proteins expression of fibroblast related to adhesion in response to different transmucosal abutment surfaces
Auteurs : Yeon-Hee Moon [Corée du Sud] ; Mi-Kyeong Yoon [Corée du Sud] ; Jung-Sun Moon [Corée du Sud] ; Jee-Hae Kang [Corée du Sud] ; Sun-Hun Kim [Corée du Sud] ; Hong-Seo Yang [Corée du Sud] ; Min-Seok Kim [Corée du Sud]Source :
- The Journal of Advanced Prosthodontics [ 2005-7806 ] ; 2013.
Abstract
To evaluate adherence of human gingival fibroblasts (HGFs) to transmucosal abutment of dental implant with different surface conditions with time and to investigate the roles of focal adhesion linker proteins (FALPs) involved in HGFs adhesion to abutment surfaces.
Morphologies of cultured HGFs on titanium and ceramic discs with different surface were observed by scanning electron microscopy. Biocompatibility and focal adhesion were evaluated by ultrasonic wave application and cell viability assay. FALPs expression levels were assessed by RT-PCR and western blot.
There seemed to be little difference in biocompatibility and adhesion strength of HGFs depending on the surface conditions and materials. In all experimental groups, the number of cells remaining on the disc surface after ultrasonic wave application increased more than 2 times at 3 days after seeding compared to 1-day cultured cells and this continued until 7 days of culture. FALPs expression levels, especially of vinculin and paxillin, also increased in 5-day cultured cells compared to 1-day cultured fibroblasts on the disc surface.
These results might suggest that the strength of adhesion of fibroblasts to transmucosal abutment surfaces increases with time and it seemed to be related to expressions of FALPs.
Url:
DOI: 10.4047/jap.2013.5.3.341
PubMed: 24049577
PubMed Central: 3774950
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National University, Gwangju, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju</wicri:regionArea><wicri:noRegion>Gwangju</wicri:noRegion></affiliation></author><author><name sortKey="Moon, Jung Sun" sort="Moon, Jung Sun" uniqKey="Moon J" first="Jung-Sun" last="Moon">Jung-Sun Moon</name><affiliation wicri:level="1"><nlm:aff id="A1">Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju</wicri:regionArea><wicri:noRegion>Gwangju</wicri:noRegion></affiliation></author><author><name sortKey="Kang, Jee Hae" sort="Kang, Jee Hae" uniqKey="Kang J" first="Jee-Hae" last="Kang">Jee-Hae 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expression of fibroblast related to adhesion in response to different transmucosal abutment surfaces</title><author><name sortKey="Moon, Yeon Hee" sort="Moon, Yeon Hee" uniqKey="Moon Y" first="Yeon-Hee" last="Moon">Yeon-Hee Moon</name><affiliation wicri:level="1"><nlm:aff id="A1">Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju</wicri:regionArea><wicri:noRegion>Gwangju</wicri:noRegion></affiliation></author><author><name sortKey="Yoon, Mi Kyeong" sort="Yoon, Mi Kyeong" uniqKey="Yoon M" first="Mi-Kyeong" last="Yoon">Mi-Kyeong Yoon</name><affiliation wicri:level="1"><nlm:aff id="A1">Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju</wicri:regionArea><wicri:noRegion>Gwangju</wicri:noRegion></affiliation></author><author><name sortKey="Moon, Jung Sun" sort="Moon, Jung Sun" uniqKey="Moon J" first="Jung-Sun" last="Moon">Jung-Sun Moon</name><affiliation wicri:level="1"><nlm:aff id="A1">Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju</wicri:regionArea><wicri:noRegion>Gwangju</wicri:noRegion></affiliation></author><author><name sortKey="Kang, Jee Hae" sort="Kang, Jee Hae" uniqKey="Kang J" first="Jee-Hae" last="Kang">Jee-Hae Kang</name><affiliation wicri:level="1"><nlm:aff id="A1">Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju</wicri:regionArea><wicri:noRegion>Gwangju</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Sun Hun" sort="Kim, Sun Hun" uniqKey="Kim S" first="Sun-Hun" last="Kim">Sun-Hun Kim</name><affiliation wicri:level="1"><nlm:aff id="A1">Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju</wicri:regionArea><wicri:noRegion>Gwangju</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Hong Seo" sort="Yang, Hong Seo" uniqKey="Yang H" first="Hong-Seo" last="Yang">Hong-Seo Yang</name><affiliation wicri:level="1"><nlm:aff id="A1">Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju</wicri:regionArea><wicri:noRegion>Gwangju</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Min Seok" sort="Kim, Min Seok" uniqKey="Kim M" first="Min-Seok" last="Kim">Min-Seok Kim</name><affiliation wicri:level="1"><nlm:aff id="A1">Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju</wicri:regionArea><wicri:noRegion>Gwangju</wicri:noRegion></affiliation></author></analytic><series><title level="j">The Journal of Advanced Prosthodontics</title><idno type="ISSN">2005-7806</idno><idno type="eISSN">2005-7814</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>PURPOSE</title><p>To evaluate adherence of human gingival fibroblasts (HGFs) to transmucosal abutment of dental implant with different surface conditions with time and to investigate the roles of focal adhesion linker proteins (FALPs) involved in HGFs adhesion to abutment surfaces.</p></sec><sec><title>MATERIALS AND METHODS</title><p>Morphologies of cultured HGFs on titanium and ceramic discs with different surface were observed by scanning electron microscopy. Biocompatibility and focal adhesion were evaluated by ultrasonic wave application and cell viability assay. FALPs expression levels were assessed by RT-PCR and western blot.</p></sec><sec><title>RESULTS</title><p>There seemed to be little difference in biocompatibility and adhesion strength of HGFs depending on the surface conditions and materials. In all experimental groups, the number of cells remaining on the disc surface after ultrasonic wave application increased more than 2 times at 3 days after seeding compared to 1-day cultured cells and this continued until 7 days of culture. FALPs expression levels, especially of vinculin and paxillin, also increased in 5-day cultured cells compared to 1-day cultured fibroblasts on the disc surface.</p></sec><sec><title>CONCLUSION</title><p>These results might suggest that the strength of adhesion of fibroblasts to transmucosal abutment surfaces increases with time and it seemed to be related to expressions of FALPs.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Palma Carri, C" uniqKey="Palma Carri C">C Palma-Carrió</name></author><author><name sortKey="Balaguer Martinez, J" uniqKey="Balaguer Martinez J">J Balaguer-Martínez</name></author><author><name sortKey="Pe Arrocha Oltra, D" uniqKey="Pe Arrocha Oltra D">D Peñarrocha-Oltra</name></author><author><name sortKey="Pe Arrocha Diago, M" uniqKey="Pe Arrocha Diago M">M Peñarrocha-Diago</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Berglundh, T" uniqKey="Berglundh T">T 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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Adv Prosthodont</journal-id><journal-id journal-id-type="iso-abbrev">J Adv Prosthodont</journal-id><journal-id journal-id-type="publisher-id">JAP</journal-id><journal-title-group><journal-title>The Journal of Advanced Prosthodontics</journal-title></journal-title-group><issn pub-type="ppub">2005-7806</issn><issn pub-type="epub">2005-7814</issn><publisher><publisher-name>The Korean Academy of Prosthodontics</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24049577</article-id><article-id pub-id-type="pmc">3774950</article-id><article-id pub-id-type="doi">10.4047/jap.2013.5.3.341</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Focal adhesion linker proteins expression of fibroblast related to adhesion in response to different transmucosal abutment surfaces</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Moon</surname><given-names>Yeon-Hee</given-names></name><degrees>MS</degrees><xref ref-type="aff" rid="A1"></xref></contrib><contrib contrib-type="author"><name><surname>Yoon</surname><given-names>Mi-Kyeong</given-names></name><degrees>DDS</degrees><degrees>PhD</degrees><xref ref-type="aff" rid="A1"></xref></contrib><contrib contrib-type="author"><name><surname>Moon</surname><given-names>Jung-Sun</given-names></name><degrees>PhD</degrees><xref ref-type="aff" rid="A1"></xref></contrib><contrib contrib-type="author"><name><surname>Kang</surname><given-names>Jee-Hae</given-names></name><degrees>MS</degrees><xref ref-type="aff" rid="A1"></xref></contrib><contrib contrib-type="author"><name><surname>Kim</surname><given-names>Sun-Hun</given-names></name><degrees>DDS</degrees><degrees>PhD</degrees><xref ref-type="aff" rid="A1"></xref></contrib><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Hong-Seo</given-names></name><degrees>DDS</degrees><degrees>PhD</degrees><xref ref-type="aff" rid="A1"></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Kim</surname><given-names>Min-Seok</given-names></name><degrees>DDS</degrees><degrees>PhD</degrees><xref ref-type="aff" rid="A1"></xref></contrib></contrib-group><aff id="A1">Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.</aff><author-notes><corresp>Corresponding author: Min-Seok Kim. Dental Science Research Institute, School of Dentistry, Chonnam National University, Yongbongdong, Gwangju, 500-757, Republic of Korea. Tel: 82625304823, <email>greatone@chonnam.ac.kr</email></corresp></author-notes><pub-date pub-type="ppub"><month>8</month><year>2013</year></pub-date><pub-date pub-type="epub"><day>31</day><month>8</month><year>2013</year></pub-date><volume>5</volume><issue>3</issue><fpage>341</fpage><lpage>350</lpage><history><date date-type="received"><day>24</day><month>4</month><year>2013</year></date><date date-type="rev-recd"><day>20</day><month>7</month><year>2013</year></date><date date-type="accepted"><day>06</day><month>8</month><year>2013</year></date></history><permissions><copyright-statement>© 2013 The Korean Academy of Prosthodontics</copyright-statement><copyright-year>2013</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">http://creativecommons.org/licenses/by-nc/3.0/</ext-link>) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><sec><title>PURPOSE</title><p>To evaluate adherence of human gingival fibroblasts (HGFs) to transmucosal abutment of dental implant with different surface conditions with time and to investigate the roles of focal adhesion linker proteins (FALPs) involved in HGFs adhesion to abutment surfaces.</p></sec><sec><title>MATERIALS AND METHODS</title><p>Morphologies of cultured HGFs on titanium and ceramic discs with different surface were observed by scanning electron microscopy. Biocompatibility and focal adhesion were evaluated by ultrasonic wave application and cell viability assay. FALPs expression levels were assessed by RT-PCR and western blot.</p></sec><sec><title>RESULTS</title><p>There seemed to be little difference in biocompatibility and adhesion strength of HGFs depending on the surface conditions and materials. In all experimental groups, the number of cells remaining on the disc surface after ultrasonic wave application increased more than 2 times at 3 days after seeding compared to 1-day cultured cells and this continued until 7 days of culture. FALPs expression levels, especially of vinculin and paxillin, also increased in 5-day cultured cells compared to 1-day cultured fibroblasts on the disc surface.</p></sec><sec><title>CONCLUSION</title><p>These results might suggest that the strength of adhesion of fibroblasts to transmucosal abutment surfaces increases with time and it seemed to be related to expressions of FALPs.</p></sec></abstract><kwd-group><kwd>Transmucosal abutment</kwd><kwd>Focal adhesion linker proteins</kwd><kwd>Gingival fibroblast</kwd><kwd>Adhesion</kwd></kwd-group></article-meta></front><floats-group><fig id="F1" orientation="portrait" position="float"><label>Fig. 1</label><caption><p>Photographs of surface modified titanium and ceramic discs with or without HGF taken using SEM: (A, F, K) machined titanium surface (MT), (B, G, I) polished titanium surface (PT), (C, H, M) titanium coated with titanium nitride (CT), (D, I, N) machined ceramic surface (MC), (E, J, O) polished ceramic surface (PC), (C, H, M). HGFs were cultured for 1 day (F-J) and for 5 days (K-O), respectively. Magnification: ×200.</p></caption><graphic xlink:href="jap-5-341-g001"></graphic></fig><fig id="F2" orientation="portrait" position="float"><label>Fig. 2</label><caption><p>Growth of HGFs on five discs with different modified surfaces was assessed using the MTT assay. Results are shown as the mean number of cells from four wells ±SD. Ctrl: control, MT: machined titanium surface, PT: polished titanium surface, CT: titanium coated with titanium nitride, MC: machined ceramic surface, PC: polished ceramic surface.</p></caption><graphic xlink:href="jap-5-341-g002"></graphic></fig><fig id="F3" orientation="portrait" position="float"><label>Fig. 3</label><caption><p>Comparison of cell survival rates of HGFs cultured on five discs with different modified surfaces for 1, 3, 5 and 7 days before and after mechanical force application using ultrasonic waves. Results are shown as the mean number of cells from four wells ±SD. Ctrl: control, MT: machined titanium surface, PT: polished titanium surface, CT: titanium coated with titanium nitride, MC: machined ceramic surface, PC: polished ceramic surface.</p></caption><graphic xlink:href="jap-5-341-g003"></graphic></fig><fig id="F4" orientation="portrait" position="float"><label>Fig. 4</label><caption><p>mRNA expressions of focal adhesion linker proteins of cultured HGFs grown on five discs with different modified surfaces assessed by semi-quantitative RT-PCR assay. (A) Comparison of mRNA expression levels of focal adhesion linker proteins obtained from 5-day and 7-day cultured HGFs grown on five discs with different modified surfaces. (B) Changes in mRNA expression levels of focal adhesion linker proteins with time. Ctrl: control, MT: machined titanium surface, PT: polished titanium surface, CT: titanium coated with titanium nitride, MC: machined ceramic surface, PC: polished ceramic surface.</p></caption><graphic xlink:href="jap-5-341-g004"></graphic></fig><fig id="F5" orientation="portrait" position="float"><label>Fig. 5</label><caption><p>Changes in expression of paxillin and vinculin proteins in HGFs cultured for 1 day and 5 days respectively on five discs with different modified surfaces assessed using western blot. Ctrl: control, MT: machined titanium surface, PT: polished titanium surface, CT: titanium coated with titanium nitride, MC: machined ceramic surface, PC: polished ceramic surface.</p></caption><graphic xlink:href="jap-5-341-g005"></graphic></fig><table-wrap id="T1" orientation="portrait" position="float"><label>Table 1</label><caption><p>Primer sequences using the RT-PCR</p></caption><graphic xlink:href="jap-5-341-i001"></graphic><table-wrap-foot><fn><p><sup>*</sup>NIH Gene bank accession number</p></fn></table-wrap-foot></table-wrap></floats-group></pmc><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Moon, Yeon Hee" sort="Moon, Yeon Hee" uniqKey="Moon Y" first="Yeon-Hee" last="Moon">Yeon-Hee Moon</name></noRegion><name sortKey="Kang, Jee Hae" sort="Kang, Jee Hae" uniqKey="Kang J" first="Jee-Hae" last="Kang">Jee-Hae Kang</name><name sortKey="Kim, Min Seok" sort="Kim, Min Seok" uniqKey="Kim M" first="Min-Seok" last="Kim">Min-Seok Kim</name><name sortKey="Kim, Sun Hun" sort="Kim, Sun Hun" uniqKey="Kim S" first="Sun-Hun" last="Kim">Sun-Hun Kim</name><name sortKey="Moon, Jung Sun" sort="Moon, Jung Sun" uniqKey="Moon J" first="Jung-Sun" last="Moon">Jung-Sun Moon</name><name sortKey="Yang, Hong Seo" sort="Yang, Hong Seo" uniqKey="Yang H" first="Hong-Seo" last="Yang">Hong-Seo Yang</name><name sortKey="Yoon, Mi Kyeong" sort="Yoon, Mi Kyeong" uniqKey="Yoon M" first="Mi-Kyeong" last="Yoon">Mi-Kyeong Yoon</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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