Titanium nanotubes activate genes related to bone formation in vitro
Identifieur interne : 004783 ( Ncbi/Merge ); précédent : 004782; suivant : 004784Titanium nanotubes activate genes related to bone formation in vitro
Auteurs : Alfonso Pozio [Italie] ; Annalisa Palmieri [Italie] ; Ambra Girardi [Italie] ; Francesca Cura [Italie] ; Francesco Carinci [Italie]Source :
- Dental Research Journal [ 1735-3327 ] ; 2012.
Abstract
Titanium is used worldwide to make osseointegrable devices, thanks to its favorable characteristics as mechanical proprieties and biocompatibility, demonstrated by
To investigate how titanium nanotubes stimulate osteoblasts differentiation and proliferation, some osteoblast genes (SP7, RUNX2, COL3A1, COL1A1, ALPL, SPP1 and FOSL1) were analyzed by quantitative Real Time RT- PCR.
After 15 days, osteoblasts cultivated on titanium naotube showed the up-regulation of bone related genes SP7, ENG, FOSL1 and SPP1 and the down-regulation of RUNX2, COL3A1, COL1A1, and ALPL. After 30 days of treatment, the bone related genes SP7, ENG, FOSL1 and RUNX2 were up-regulated while COL3A1, COL1A1, ALPL and SPP1 were down-regulated.
Our results, demonstrates that titanium nanotubes can lead to osteoblast differentiation and extracellular matrix deposition and mineralization in dental pulp stem cells by the activation of osteoblast related genes SPP1, FOSL1 and RUNX2.
Url:
DOI: 10.4103/1735-3327.109736
PubMed: 23814577
PubMed Central: 3692167
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<series><title level="j">Dental Research Journal</title>
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<front><div type="abstract" xml:lang="en"><sec id="st1"><title>Background:</title>
<p>Titanium is used worldwide to make osseointegrable devices, thanks to its favorable characteristics as mechanical proprieties and biocompatibility, demonstrated by <italic>in vivo</italic>
studies with animal models and clinical trials over a forty-year period. However, the exact genetic effect of the titanium layer on cells is still not well characterized.</p>
</sec>
<sec id="st2"><title>Materials and Methods:</title>
<p>To investigate how titanium nanotubes stimulate osteoblasts differentiation and proliferation, some osteoblast genes (SP7, RUNX2, COL3A1, COL1A1, ALPL, SPP1 and FOSL1) were analyzed by quantitative Real Time RT- PCR.</p>
</sec>
<sec id="st3"><title>Results:</title>
<p>After 15 days, osteoblasts cultivated on titanium naotube showed the up-regulation of bone related genes SP7, ENG, FOSL1 and SPP1 and the down-regulation of RUNX2, COL3A1, COL1A1, and ALPL. After 30 days of treatment, the bone related genes SP7, ENG, FOSL1 and RUNX2 were up-regulated while COL3A1, COL1A1, ALPL and SPP1 were down-regulated.</p>
</sec>
<sec id="st4"><title>Conclusions:</title>
<p>Our results, demonstrates that titanium nanotubes can lead to osteoblast differentiation and extracellular matrix deposition and mineralization in dental pulp stem cells by the activation of osteoblast related genes SPP1, FOSL1 and RUNX2.</p>
</sec>
</div>
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<biblStruct><analytic><author><name sortKey="Ohtsuki, C" uniqKey="Ohtsuki C">C Ohtsuki</name>
</author>
<author><name sortKey="Kamitakahara, M" uniqKey="Kamitakahara M">M Kamitakahara</name>
</author>
<author><name sortKey="Miyazaki, T" uniqKey="Miyazaki T">T Miyazaki</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ziros, Pg" uniqKey="Ziros P">PG Ziros</name>
</author>
<author><name sortKey="Basdra, Ek" uniqKey="Basdra E">EK Basdra</name>
</author>
<author><name sortKey="Papavassiliou, Ag" uniqKey="Papavassiliou A">AG Papavassiliou</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Dent Res J (Isfahan)</journal-id>
<journal-id journal-id-type="iso-abbrev">Dent Res J (Isfahan)</journal-id>
<journal-id journal-id-type="publisher-id">DRJ</journal-id>
<journal-title-group><journal-title>Dental Research Journal</journal-title>
</journal-title-group>
<issn pub-type="ppub">1735-3327</issn>
<issn pub-type="epub">2008-0255</issn>
<publisher><publisher-name>Medknow Publications & Media Pvt Ltd</publisher-name>
<publisher-loc>India</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">23814577</article-id>
<article-id pub-id-type="pmc">3692167</article-id>
<article-id pub-id-type="publisher-id">DRJ-9-164</article-id>
<article-id pub-id-type="doi">10.4103/1735-3327.109736</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Titanium nanotubes activate genes related to bone formation <italic>in vitro</italic>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Pozio</surname>
<given-names>Alfonso</given-names>
</name>
<xref ref-type="aff" rid="aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Palmieri</surname>
<given-names>Annalisa</given-names>
</name>
<xref ref-type="aff" rid="aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Girardi</surname>
<given-names>Ambra</given-names>
</name>
<xref ref-type="aff" rid="aff3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Cura</surname>
<given-names>Francesca</given-names>
</name>
<xref ref-type="aff" rid="aff3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Carinci</surname>
<given-names>Francesco</given-names>
</name>
<xref ref-type="aff" rid="aff2">2</xref>
<xref ref-type="corresp" rid="cor1"></xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>1</label>
ENEA, IDROCOMB, C.R. Casaccia, Rome, Italy</aff>
<aff id="aff2"><label>2</label>
Department of Medical-Surgical Sciences of Communication and Behavior, Section of Maxillofacial and Plastic Surgery, University of Ferrara, Ferrara, Italy</aff>
<aff id="aff3"><label>3</label>
Department of Histology, Embryology and Applied Biology, Centre of Molecular Genetics, CARISBO Foundation, University of Bologna, Bologna, Italy</aff>
<author-notes><corresp id="cor1"><bold>Address for correspondence:</bold>
Prof. Francesco Carinci, Department of D.M.C.C.C., Section of Maxillofacial and Plastic Surgery, University of Ferrara, Corso Giovecca 203, 44100 Ferrara, Italy. <bold>E-mail:</bold>
<email xlink:href="crc@unife.it">crc@unife.it</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>12</month>
<year>2012</year>
</pub-date>
<volume>9</volume>
<issue>Suppl 2</issue>
<fpage>S164</fpage>
<lpage>S168</lpage>
<history><date date-type="received"><month>7</month>
<year>2012</year>
</date>
<date date-type="accepted"><month>10</month>
<year>2012</year>
</date>
</history>
<permissions><copyright-statement>Copyright: © Dental Research Journal</copyright-statement>
<copyright-year>2012</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
</license>
</permissions>
<abstract><sec id="st1"><title>Background:</title>
<p>Titanium is used worldwide to make osseointegrable devices, thanks to its favorable characteristics as mechanical proprieties and biocompatibility, demonstrated by <italic>in vivo</italic>
studies with animal models and clinical trials over a forty-year period. However, the exact genetic effect of the titanium layer on cells is still not well characterized.</p>
</sec>
<sec id="st2"><title>Materials and Methods:</title>
<p>To investigate how titanium nanotubes stimulate osteoblasts differentiation and proliferation, some osteoblast genes (SP7, RUNX2, COL3A1, COL1A1, ALPL, SPP1 and FOSL1) were analyzed by quantitative Real Time RT- PCR.</p>
</sec>
<sec id="st3"><title>Results:</title>
<p>After 15 days, osteoblasts cultivated on titanium naotube showed the up-regulation of bone related genes SP7, ENG, FOSL1 and SPP1 and the down-regulation of RUNX2, COL3A1, COL1A1, and ALPL. After 30 days of treatment, the bone related genes SP7, ENG, FOSL1 and RUNX2 were up-regulated while COL3A1, COL1A1, ALPL and SPP1 were down-regulated.</p>
</sec>
<sec id="st4"><title>Conclusions:</title>
<p>Our results, demonstrates that titanium nanotubes can lead to osteoblast differentiation and extracellular matrix deposition and mineralization in dental pulp stem cells by the activation of osteoblast related genes SPP1, FOSL1 and RUNX2.</p>
</sec>
</abstract>
<kwd-group><kwd>Gene expression</kwd>
<kwd>nanotubes</kwd>
<kwd>osteoblasts</kwd>
<kwd>titanium disks</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Italie</li>
</country>
<region><li>Latium</li>
</region>
<settlement><li>Rome</li>
</settlement>
</list>
<tree><country name="Italie"><region name="Latium"><name sortKey="Pozio, Alfonso" sort="Pozio, Alfonso" uniqKey="Pozio A" first="Alfonso" last="Pozio">Alfonso Pozio</name>
</region>
<name sortKey="Carinci, Francesco" sort="Carinci, Francesco" uniqKey="Carinci F" first="Francesco" last="Carinci">Francesco Carinci</name>
<name sortKey="Cura, Francesca" sort="Cura, Francesca" uniqKey="Cura F" first="Francesca" last="Cura">Francesca Cura</name>
<name sortKey="Girardi, Ambra" sort="Girardi, Ambra" uniqKey="Girardi A" first="Ambra" last="Girardi">Ambra Girardi</name>
<name sortKey="Palmieri, Annalisa" sort="Palmieri, Annalisa" uniqKey="Palmieri A" first="Annalisa" last="Palmieri">Annalisa Palmieri</name>
</country>
</tree>
</affiliations>
</record>
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