Development of a Contemporary Animal Model of Candida albicans-Associated Denture Stomatitis Using a Novel Intraoral Denture System
Identifieur interne : 003C58 ( Ncbi/Merge ); précédent : 003C57; suivant : 003C59Development of a Contemporary Animal Model of Candida albicans-Associated Denture Stomatitis Using a Novel Intraoral Denture System
Auteurs : Clorinda C. Johnson [États-Unis] ; Alika Yu [États-Unis] ; Heeje Lee [États-Unis] ; Paul L. Fidel [États-Unis] ; Mairi C. Noverr [États-Unis]Source :
- Infection and Immunity [ 0019-9567 ] ; 2012.
Abstract
Denture stomatitis (DS) is a fungal infection characterized by inflammation of the oral mucosa in direct contact with the denture and affects up to 50% of denture wearers. Despite the prevalence, very little is known about the role of fungal or host factors that contribute to pathogenesis. Recently, we developed a novel intraoral denture system for rodent research. This denture system consists of custom-fitted fixed and removable parts to allow repeated sampling and longitudinal studies. The purpose of this study was to use this denture system to develop a clinically relevant animal model of DS. To establish DS, rats were inoculated with pelleted
Url:
DOI: 10.1128/IAI.00019-12
PubMed: 22392931
PubMed Central: 3347462
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<front><div type="abstract" xml:lang="en"><p>Denture stomatitis (DS) is a fungal infection characterized by inflammation of the oral mucosa in direct contact with the denture and affects up to 50% of denture wearers. Despite the prevalence, very little is known about the role of fungal or host factors that contribute to pathogenesis. Recently, we developed a novel intraoral denture system for rodent research. This denture system consists of custom-fitted fixed and removable parts to allow repeated sampling and longitudinal studies. The purpose of this study was to use this denture system to develop a clinically relevant animal model of DS. To establish DS, rats were inoculated with pelleted <named-content content-type="genus-species">Candida albicans</named-content>
, which resulted in sustained colonization of the denture and palate for 8 weeks postinoculation. Biofilm formation on the denture was observed by week 4 and on the palate by week 6 postinoculation. Rats were monitored for clinical signs of disease by assigning a clinical score after macroscopic examination of the palate tissue according to Newton's method. By week 4 postinoculation, the majority of inoculated rats with dentures exhibited a clinical score of 1 (pinpoint erythema). By week 6 and week 8 postinoculation, increasing percentages of rats exhibited a clinical score of 2 (diffuse erythema/edema). Histological analysis of palate tissue demonstrated progressively increasing inflammatory cell recruitment throughout the time course of the infection. Palatal biofilm formation was commensurate with development of palatal erythema, which suggests a role for biofilm in the inflammatory response.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Infect Immun</journal-id>
<journal-id journal-id-type="iso-abbrev">Infect. Immun</journal-id>
<journal-id journal-id-type="hwp">iai</journal-id>
<journal-id journal-id-type="pmc">iai</journal-id>
<journal-id journal-id-type="publisher-id">IAI</journal-id>
<journal-title-group><journal-title>Infection and Immunity</journal-title>
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<issn pub-type="ppub">0019-9567</issn>
<issn pub-type="epub">1098-5522</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
<publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">22392931</article-id>
<article-id pub-id-type="pmc">3347462</article-id>
<article-id pub-id-type="publisher-id">00019-12</article-id>
<article-id pub-id-type="doi">10.1128/IAI.00019-12</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Fungal and Parasitic Infections</subject>
</subj-group>
</article-categories>
<title-group><article-title>Development of a Contemporary Animal Model of <named-content content-type="genus-species">Candida albicans</named-content>
-Associated Denture Stomatitis Using a Novel Intraoral Denture System</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Johnson</surname>
<given-names>Clorinda C.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yu</surname>
<given-names>Alika</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lee</surname>
<given-names>Heeje</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Fidel</surname>
<given-names>Paul L.</given-names>
<suffix>Jr</suffix>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Noverr</surname>
<given-names>Mairi C.</given-names>
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<xref ref-type="aff" rid="aff1"><sup>a</sup>
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<aff id="aff1"><label>a</label>
Department of Oral and Craniofacial Biology, Louisiana State University Health Sciences Center School of Dentistry, New Orleans, Louisiana, USA</aff>
<aff id="aff2"><label>b</label>
Department of Prosthodontics, Louisiana State University Health Sciences Center School of Dentistry, New Orleans, Louisiana, USA</aff>
</contrib-group>
<contrib-group><contrib contrib-type="editor"><name><surname>Deepe</surname>
<given-names>G. S.</given-names>
<suffix>Jr.</suffix>
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<role>Editor</role>
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</contrib-group>
<author-notes><corresp>Address correspondence to Mairi C. Noverrr, <email>mnover@lsuhsc.edu</email>
.</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>5</month>
<year>2012</year>
</pub-date>
<volume>80</volume>
<issue>5</issue>
<fpage>1736</fpage>
<lpage>1743</lpage>
<history><date date-type="received"><day>6</day>
<month>1</month>
<year>2012</year>
</date>
<date date-type="rev-request"><day>20</day>
<month>1</month>
<year>2012</year>
</date>
<date date-type="accepted"><day>28</day>
<month>2</month>
<year>2012</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2012, American Society for Microbiology. All Rights Reserved.</copyright-statement>
<copyright-year>2012</copyright-year>
<copyright-holder>American Society for Microbiology</copyright-holder>
</permissions>
<self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zii00512001736.pdf"></self-uri>
<abstract><p>Denture stomatitis (DS) is a fungal infection characterized by inflammation of the oral mucosa in direct contact with the denture and affects up to 50% of denture wearers. Despite the prevalence, very little is known about the role of fungal or host factors that contribute to pathogenesis. Recently, we developed a novel intraoral denture system for rodent research. This denture system consists of custom-fitted fixed and removable parts to allow repeated sampling and longitudinal studies. The purpose of this study was to use this denture system to develop a clinically relevant animal model of DS. To establish DS, rats were inoculated with pelleted <named-content content-type="genus-species">Candida albicans</named-content>
, which resulted in sustained colonization of the denture and palate for 8 weeks postinoculation. Biofilm formation on the denture was observed by week 4 and on the palate by week 6 postinoculation. Rats were monitored for clinical signs of disease by assigning a clinical score after macroscopic examination of the palate tissue according to Newton's method. By week 4 postinoculation, the majority of inoculated rats with dentures exhibited a clinical score of 1 (pinpoint erythema). By week 6 and week 8 postinoculation, increasing percentages of rats exhibited a clinical score of 2 (diffuse erythema/edema). Histological analysis of palate tissue demonstrated progressively increasing inflammatory cell recruitment throughout the time course of the infection. Palatal biofilm formation was commensurate with development of palatal erythema, which suggests a role for biofilm in the inflammatory response.</p>
</abstract>
</article-meta>
</front>
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<affiliations><list><country><li>États-Unis</li>
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<tree><country name="États-Unis"><region name="Louisiane"><name sortKey="Johnson, Clorinda C" sort="Johnson, Clorinda C" uniqKey="Johnson C" first="Clorinda C." last="Johnson">Clorinda C. Johnson</name>
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<name sortKey="Fidel, Paul L" sort="Fidel, Paul L" uniqKey="Fidel P" first="Paul L." last="Fidel">Paul L. Fidel</name>
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<name sortKey="Yu, Alika" sort="Yu, Alika" uniqKey="Yu A" first="Alika" last="Yu">Alika Yu</name>
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