Regulatory effect of monocytes on T cell proliferative responses to oral microbial antigens.
Identifieur interne : 008F91 ( Ncbi/Curation ); précédent : 008F90; suivant : 008F92Regulatory effect of monocytes on T cell proliferative responses to oral microbial antigens.
Auteurs : P. StashenkoSource :
- Infection and Immunity [ 0019-9567 ] ; 1982.
Abstract
Mononuclear cell preparations isolated by Ficoll-Hypaque centrifugation from human peripheral blood were found to vary considerably in the number of monocytes they contained (mean, 20.3%; range, 13 to 33%). The regulatory role of monocytes in T cell proliferative responses to sonic extracts of a panel of oral microorganisms was therefore investigated. T cells were fractionated by anti-immunoglobulin chromatography and depleted of monocytes by treatment with a monoclonal anti-human Ia-like (DR locus antigen) antibody and complement. Purified populations of monocytes were obtained by extensive adherence procedures. The resultant cell populations were greater than 95% pure, as judged by indirect immunofluorescence on a fluorescence-activated cell sorter. Monocyte-depleted T cells failed to respond by proliferation to the nonoral antigen tetanus toxoid, as well as to any oral microorganism, but retained responsiveness to phytohemagglutinin. Readdition of monocytes in final concentrations of from 5 to 15% resulted in the restoration of maximal T cell proliferation. Monocytes in greater numbers suppressed T cell responses to all sonic extracts tested.
Url:
PubMed: 6984019
PubMed Central: 347840
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001541
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :001541
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :002992
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :008F91
Links to Exploration step
PMC:347840Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Regulatory effect of monocytes on T cell proliferative responses to oral microbial antigens.</title><author><name sortKey="Stashenko, P" sort="Stashenko, P" uniqKey="Stashenko P" first="P" last="Stashenko">P. Stashenko</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">6984019</idno><idno type="pmc">347840</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC347840</idno><idno type="RBID">PMC:347840</idno><date when="1982">1982</date><idno type="wicri:Area/Pmc/Corpus">001541</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001541</idno><idno type="wicri:Area/Pmc/Curation">001541</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001541</idno><idno type="wicri:Area/Pmc/Checkpoint">002992</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002992</idno><idno type="wicri:Area/Ncbi/Merge">008F91</idno><idno type="wicri:Area/Ncbi/Curation">008F91</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Regulatory effect of monocytes on T cell proliferative responses to oral microbial antigens.</title><author><name sortKey="Stashenko, P" sort="Stashenko, P" uniqKey="Stashenko P" first="P" last="Stashenko">P. Stashenko</name></author></analytic><series><title level="j">Infection and Immunity</title><idno type="ISSN">0019-9567</idno><idno type="eISSN">1098-5522</idno><imprint><date when="1982">1982</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Mononuclear cell preparations isolated by Ficoll-Hypaque centrifugation from human peripheral blood were found to vary considerably in the number of monocytes they contained (mean, 20.3%; range, 13 to 33%). The regulatory role of monocytes in T cell proliferative responses to sonic extracts of a panel of oral microorganisms was therefore investigated. T cells were fractionated by anti-immunoglobulin chromatography and depleted of monocytes by treatment with a monoclonal anti-human Ia-like (DR locus antigen) antibody and complement. Purified populations of monocytes were obtained by extensive adherence procedures. The resultant cell populations were greater than 95% pure, as judged by indirect immunofluorescence on a fluorescence-activated cell sorter. Monocyte-depleted T cells failed to respond by proliferation to the nonoral antigen tetanus toxoid, as well as to any oral microorganism, but retained responsiveness to phytohemagglutinin. Readdition of monocytes in final concentrations of from 5 to 15% resulted in the restoration of maximal T cell proliferation. Monocytes in greater numbers suppressed T cell responses to all sonic extracts tested.</p></div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/EdenteV2/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 008F91 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 008F91 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Santé
|area= EdenteV2
|flux= Ncbi
|étape= Curation
|type= RBID
|clé= PMC:347840
|texte= Regulatory effect of monocytes on T cell proliferative responses to oral microbial antigens.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:6984019" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \
| NlmPubMed2Wicri -a EdenteV2
| This area was generated with Dilib version V0.6.32. |  |