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Biofilm Formation by the Fungal Pathogen Candida albicans: Development, Architecture, and Drug Resistance

Identifieur interne : 000669 ( Ncbi/Curation ); précédent : 000668; suivant : 000670

Biofilm Formation by the Fungal Pathogen Candida albicans: Development, Architecture, and Drug Resistance

Auteurs : Jyotsna Chandra ; Duncan M. Kuhn ; Pranab K. Mukherjee ; Lois L. Hoyer ; Thomas Mccormick ; Mahmoud A. Ghannoum

Source :

Journal of Bacteriology [ 0021-9193 ] ; 2001.

RBID : PMC:95423

Abstract

Biofilms are a protected niche for microorganisms, where they are safe from antibiotic treatment and can create a source of persistent infection. Using two clinically relevant Candida albicans biofilm models formed on bioprosthetic materials, we demonstrated that biofilm formation proceeds through three distinct developmental phases. These growth phases transform adherent blastospores to well-defined cellular communities encased in a polysaccharide matrix. Fluorescence and confocal scanning laser microscopy revealed that C. albicans biofilms have a highly heterogeneous architecture composed of cellular and noncellular elements. In both models, antifungal resistance of biofilm-grown cells increased in conjunction with biofilm formation. The expression of agglutinin-like (ALS) genes, which encode a family of proteins implicated in adhesion to host surfaces, was differentially regulated between planktonic and biofilm-grown cells. The ability of C. albicans to form biofilms contrasts sharply with that of Saccharomyces cerevisiae, which adhered to bioprosthetic surfaces but failed to form a mature biofilm. The studies described here form the basis for investigations into the molecular mechanisms of Candida biofilm biology and antifungal resistance and provide the means to design novel therapies for biofilm-based infections.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95423

DOI: 10.1128/JB.183.18.5385-5394.2001
PubMed: 11514524
PubMed Central: 95423

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PMC:95423

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 biofilm models formed on bioprosthetic materials, we demonstrated that biofilm formation proceeds through three distinct developmental phases. These growth phases transform adherent blastospores to well-defined cellular communities encased in a polysaccharide matrix. Fluorescence and confocal scanning laser microscopy revealed that <italic>C. albicans</italic>
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) genes, which encode a family of proteins implicated in adhesion to host surfaces, was differentially regulated between planktonic and biofilm-grown cells. The ability of <italic>C. albicans</italic>
 to form biofilms contrasts sharply with that of <italic>Saccharomyces cerevisiae</italic>
, which adhered to bioprosthetic surfaces but failed to form a mature biofilm. The studies described here form the basis for investigations into the molecular mechanisms of <italic>Candida</italic>
 biofilm biology and antifungal resistance and provide the means to design novel therapies for biofilm-based infections.</p>
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Biofilm Formation by the Fungal Pathogen Candida albicans: Development, Architecture, and Drug Resistance

Biofilm Formation by the Fungal Pathogen Candida albicans: Development, Architecture, and Drug Resistance

Source :

Abstract

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Le document en format XML

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Pour générer des pages wiki