Degradation of Levan by Actinomyces viscosus
Identifieur interne : 007575 ( Ncbi/Checkpoint ); précédent : 007574; suivant : 007576Degradation of Levan by Actinomyces viscosus
Auteurs : Chris H. Miller ; Penelope J. B. SomersSource :
- Infection and Immunity [ 0019-9567 ] ; 1978.
Abstract
Url:
PubMed: 32137
PubMed Central: 422144
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Degradation of Levan by <italic>Actinomyces viscosus</italic>
</title>
<author><name sortKey="Miller, Chris H" sort="Miller, Chris H" uniqKey="Miller C" first="Chris H." last="Miller">Chris H. Miller</name>
</author>
<author><name sortKey="Somers, Penelope J B" sort="Somers, Penelope J B" uniqKey="Somers P" first="Penelope J. B." last="Somers">Penelope J. B. Somers</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Degradation of Levan by <italic>Actinomyces viscosus</italic>
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<author><name sortKey="Miller, Chris H" sort="Miller, Chris H" uniqKey="Miller C" first="Chris H." last="Miller">Chris H. Miller</name>
</author>
<author><name sortKey="Somers, Penelope J B" sort="Somers, Penelope J B" uniqKey="Somers P" first="Penelope J. B." last="Somers">Penelope J. B. Somers</name>
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<series><title level="j">Infection and Immunity</title>
<idno type="ISSN">0019-9567</idno>
<idno type="eISSN">1098-5522</idno>
<imprint><date when="1978">1978</date>
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<front><div type="abstract" xml:lang="en"><p><italic>Actinomyces viscosus</italic>
ATCC 15987 was examined for its ability to hydrolyze its own levan. Washed whole cells and an ammonium sulfate fraction from cell-free culture fluids were shown to possess levan hydrolase activity. Analyses of reaction mixtures by gel filtration and thin-layer chromatography demonstrated that the product of levan hydrolysis was free fructose. The cell-associated and extracellular enzyme preparations also hydrolyzed inulin and the levans synthesized by <italic>Aerobacter levanicum</italic>
and <italic>Bacillus subtilis.</italic>
Growth of <italic>A. viscosus</italic>
in media supplemented with 0.1% <italic>A. viscosus</italic>
levan resulted in a 33-fold increase and a 7-fold increase in the specific activities of the respective extracellular and cell-associated enzymes when compared with those from 55 mM glucose cultures. Growth in the presence of 29.2 mM sucrose resulted in a 28-fold increase and a 5-fold increase in the specific activities of the respective enzymes when compared with those from the glucose cultures. The extracellular enzyme exhibited high activity over a wide pH range, with 87 and 89% of its pH 6.0 optimum activity at pH 5.0 and 7.0, respectively. The cell-associated enzyme also exhibited optimum activity at pH 6.0, but this was decreased to 10 and 20% at pH 5.0 and 7.0, respectively. Analysis for the presence of extracellular levan during growth of <italic>A. viscosus</italic>
in sucrose broths demonstrated that peak levan concentrations occurred during the mid-exponential to late-exponential phase of growth followed by a rapid decline in extracellular levan as a result of levan hydrolase activity.</p>
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<tree><noCountry><name sortKey="Miller, Chris H" sort="Miller, Chris H" uniqKey="Miller C" first="Chris H." last="Miller">Chris H. Miller</name>
<name sortKey="Somers, Penelope J B" sort="Somers, Penelope J B" uniqKey="Somers P" first="Penelope J. B." last="Somers">Penelope J. B. Somers</name>
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