Comparison of alkaline phosphatase activity of MC3T3-E1 cells cultured on different Ti surfaces: modified sandblasted with large grit and acid-etched (MSLA), laser-treated, and laser and acid-treated Ti surfaces
Identifieur interne : 006824 ( Ncbi/Checkpoint ); précédent : 006823; suivant : 006825Comparison of alkaline phosphatase activity of MC3T3-E1 cells cultured on different Ti surfaces: modified sandblasted with large grit and acid-etched (MSLA), laser-treated, and laser and acid-treated Ti surfaces
Auteurs : Lin-Jie Li [Corée du Sud] ; So-Nam Kim [Corée du Sud] ; Sung-Am Cho [Corée du Sud]Source :
- The Journal of Advanced Prosthodontics [ 2005-7806 ] ; 2016.
Abstract
In this study, the aim of this study was to evaluate the effect of implant surface treatment on cell differentiation of osteoblast cells. For this purpose, three surfaces were compared: (1) a modified SLA (MSLA: sand-blasted with large grit, acid-etched, and immersed in 0.9% NaCl), (2) a laser treatment (LT: laser treatment) titanium surface and (3) a laser and acid-treated (LAT: laser treatment, acid-etched) titanium surface.
The MSLA surfaces were considered as the control group, and LT and LAT surfaces as test groups. Alkaline phosphatase expression (ALP) was used to quantify osteoblastic differentiation of MC3T3-E1 cell. Surface roughness was evaluated by a contact profilometer (URFPAK-SV; Mitutoyo, Kawasaki, Japan) and characterized by two parameters: mean roughness (Ra) and maximum peak-to-valley height (Rt).
Scanning electron microscope revealed that MSLA (control group) surface was not as rough as LT, LAT surface (test groups). Alkaline phosphatase expression, the measure of osteoblastic differentiation, and total ALP expression by surface-adherent cells were found to be highest at 21 days for all three surfaces tested (
This study suggested that MSLA and LAT surfaces exhibited more favorable environment for osteoblast differentiation when compared with LT surface, the results that are important for implant surface modification studies.
Url:
DOI: 10.4047/jap.2016.8.3.235
PubMed: 27350860
PubMed Central: 4919496
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparison of alkaline phosphatase activity of MC3T3-E1 cells cultured on different Ti surfaces: modified sandblasted with large grit and acid-etched (MSLA), laser-treated, and laser and acid-treated Ti surfaces</title><author><name sortKey="Li, Lin Jie" sort="Li, Lin Jie" uniqKey="Li L" first="Lin-Jie" last="Li">Lin-Jie Li</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Dentistry, Kyungpook National University, Daegu, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Prosthodontics, College of Dentistry, Kyungpook National University, Daegu</wicri:regionArea><wicri:noRegion>Daegu</wicri:noRegion></affiliation></author><author><name sortKey="Kim, So Nam" sort="Kim, So Nam" uniqKey="Kim S" first="So-Nam" last="Kim">So-Nam Kim</name><affiliation wicri:level="1"><nlm:aff id="A2">CSM IMPLANT Surface Treatment Institute, Daegu, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>CSM IMPLANT Surface Treatment Institute, Daegu</wicri:regionArea><wicri:noRegion>Daegu</wicri:noRegion></affiliation></author><author><name sortKey="Cho, Sung Am" sort="Cho, Sung Am" uniqKey="Cho S" first="Sung-Am" last="Cho">Sung-Am Cho</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Dentistry, Kyungpook National University, Daegu, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Prosthodontics, College of Dentistry, Kyungpook National University, Daegu</wicri:regionArea><wicri:noRegion>Daegu</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27350860</idno><idno type="pmc">4919496</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919496</idno><idno type="RBID">PMC:4919496</idno><idno type="doi">10.4047/jap.2016.8.3.235</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000663</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000663</idno><idno type="wicri:Area/Pmc/Curation">000663</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000663</idno><idno type="wicri:Area/Pmc/Checkpoint">000816</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000816</idno><idno type="wicri:Area/Ncbi/Merge">006824</idno><idno type="wicri:Area/Ncbi/Curation">006824</idno><idno type="wicri:Area/Ncbi/Checkpoint">006824</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Comparison of alkaline phosphatase activity of MC3T3-E1 cells cultured on different Ti surfaces: modified sandblasted with large grit and acid-etched (MSLA), laser-treated, and laser and acid-treated Ti surfaces</title><author><name sortKey="Li, Lin Jie" sort="Li, Lin Jie" uniqKey="Li L" first="Lin-Jie" last="Li">Lin-Jie Li</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Dentistry, Kyungpook National University, Daegu, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Prosthodontics, College of Dentistry, Kyungpook National University, Daegu</wicri:regionArea><wicri:noRegion>Daegu</wicri:noRegion></affiliation></author><author><name sortKey="Kim, So Nam" sort="Kim, So Nam" uniqKey="Kim S" first="So-Nam" last="Kim">So-Nam Kim</name><affiliation wicri:level="1"><nlm:aff id="A2">CSM IMPLANT Surface Treatment Institute, Daegu, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>CSM IMPLANT Surface Treatment Institute, Daegu</wicri:regionArea><wicri:noRegion>Daegu</wicri:noRegion></affiliation></author><author><name sortKey="Cho, Sung Am" sort="Cho, Sung Am" uniqKey="Cho S" first="Sung-Am" last="Cho">Sung-Am Cho</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Dentistry, Kyungpook National University, Daegu, Republic of Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Prosthodontics, College of Dentistry, Kyungpook National University, Daegu</wicri:regionArea><wicri:noRegion>Daegu</wicri:noRegion></affiliation></author></analytic><series><title level="j">The Journal of Advanced Prosthodontics</title><idno type="ISSN">2005-7806</idno><idno type="eISSN">2005-7814</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>PURPOSE</title><p>In this study, the aim of this study was to evaluate the effect of implant surface treatment on cell differentiation of osteoblast cells. For this purpose, three surfaces were compared: (1) a modified SLA (MSLA: sand-blasted with large grit, acid-etched, and immersed in 0.9% NaCl), (2) a laser treatment (LT: laser treatment) titanium surface and (3) a laser and acid-treated (LAT: laser treatment, acid-etched) titanium surface.</p></sec><sec><title>MATERIALS AND METHODS</title><p>The MSLA surfaces were considered as the control group, and LT and LAT surfaces as test groups. Alkaline phosphatase expression (ALP) was used to quantify osteoblastic differentiation of MC3T3-E1 cell. Surface roughness was evaluated by a contact profilometer (URFPAK-SV; Mitutoyo, Kawasaki, Japan) and characterized by two parameters: mean roughness (Ra) and maximum peak-to-valley height (Rt).</p></sec><sec><title>RESULTS</title><p>Scanning electron microscope revealed that MSLA (control group) surface was not as rough as LT, LAT surface (test groups). Alkaline phosphatase expression, the measure of osteoblastic differentiation, and total ALP expression by surface-adherent cells were found to be highest at 21 days for all three surfaces tested (<italic>P</italic><.05). Furthermore, ALP expression levels of MSLA and LAT surfaces were significantly higher than expression levels of LT surface-adherent cells at 7, 14, and 21 days, respectively (<italic>P</italic><.05). However, ALP expression levels between MSLA and LAT surface were equal at 7, 14, and 21 days (<italic>P</italic>>.05).</p></sec><sec><title>CONCLUSION</title><p>This study suggested that MSLA and LAT surfaces exhibited more favorable environment for osteoblast differentiation when compared with LT surface, the results that are important for implant surface modification studies.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Sammons, Rl" uniqKey="Sammons R">RL Sammons</name></author><author><name sortKey="Lumbikanonda, N" uniqKey="Lumbikanonda N">N Lumbikanonda</name></author><author><name sortKey="Gross, M" uniqKey="Gross M">M Gross</name></author><author><name sortKey="Cantzler, P" uniqKey="Cantzler P">P Cantzler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, F" uniqKey="Zhang F">F Zhang</name></author><author><name sortKey="Yang, Gl" uniqKey="Yang G">GL 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uniqKey="Sonoda C">CK Sonoda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gyorgyey, A" uniqKey="Gyorgyey A">Á Györgyey</name></author><author><name sortKey="Ungvari, K" uniqKey="Ungvari K">K Ungvári</name></author><author><name sortKey="Kecskemeti, G" uniqKey="Kecskemeti G">G Kecskeméti</name></author><author><name sortKey="Kopniczky, J" uniqKey="Kopniczky J">J Kopniczky</name></author><author><name sortKey="Hopp, B" uniqKey="Hopp B">B Hopp</name></author><author><name sortKey="Oszk, A" uniqKey="Oszk A">A Oszkó</name></author><author><name sortKey="Pelosczi, I" uniqKey="Pelosczi I">I Pelösczi</name></author><author><name sortKey="Rakonczay, Z" uniqKey="Rakonczay Z">Z Rakonczay</name></author><author><name sortKey="Nagy, K" uniqKey="Nagy K">K Nagy</name></author><author><name sortKey="Turz, K" uniqKey="Turz K">K Turzó</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guo, Z" uniqKey="Guo Z">Z 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