Inflammatory mediators of the terminal dentition in adult and early onset periodontitis
Identifieur interne : 009F33 ( Main/Merge ); précédent : 009F32; suivant : 009F34Inflammatory mediators of the terminal dentition in adult and early onset periodontitis
Auteurs : G. E. Salvi [États-Unis, Suisse] ; C. E. Brown [États-Unis] ; K. Fujihashi [États-Unis] ; H. Kiyono [États-Unis] ; F. W. Smith [États-Unis] ; J. D. Becks [États-Unis] ; S. Offenbacher [États-Unis]Source :
- Journal of periodontal research [ 0022-3484 ] ; 1998.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Analyse quantitative, Homme.
English descriptors
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Abstract
Based upon the prosthodontic literature, subjects who are at the transition stage between natural dentition and edentulism are called "terminal dentition" (TD) cases. The aim of the present cross-sectional investigation was to characterize the local and systemic inflammatory responses in 2 groups of patients with terminal dentition periodontitis. Eight severe adult periodontitis terminal dentition (AP-TD) subjects and 8 early onset periodontitis terminal dentition (EOP-TD) subjects were entered into the study. Our purpose was to measure an extended battery of cytokines in the gingival crevicular fluid (GCF) and in lipopolysaccharide (LPS)-stimulated monocytic culture supernatants as well as gingival mononuclear cell messenger RNA (mRNA) transcripts determined from biopsy samples. Within the GCF there were 3 tiers (levels) of mediators based upon approximate 10-fold differences in concentration. The highest tier included prostaglandin E2 (PGE2), interleukin-1β (IL-1β) and interleukin-2 (IL-2), the intermediate tier included tumor necrosis factor alpha (TNFα) and interferon gamma (IFN-y) and at the lowest concentration level were interleukin-4 (IL-4) and interleukin-6 (IL-6). Thus, the GCF analysis clearly indicated that in both AP-TD and EOP-TD groups the monocytic, i.e. IL-1 and PGE2 and Thl, i.e. IL-2 and IFN-y, inflammatory mediator levels quantitatively dominated over the Th2 mediators, i.e. IL-4 and IL-6. LPS-stimulated monocytic release of IL-1β, PGE2 and TNFα was significantly elevated in both AP-TD and EOP-TD groups compared to those of a control group of 21 subjects with moderate to advanced adult periodontitis. The cytokine mRNA expression of isolated gingival mononuclear cells showed that in both the AP-TD and the EOP-TD groups Thl and Th2 cytokines were expressed, with low levels of IL-4 and IL-12. In conclusion, our data suggest that this cross-sectional TD periodontitis model may reflect progressive periodontal disease associated with tooth loss. Furthermore, although Thl cytokine levels in the GCF dominate over the Th2 response, monocytic activation provides the main source of proinflammatory mediators. In addition, LPS-stimulated peripheral blood monocytes demonstrate an upregulated inflammatory mediator secretion in the terminal dentition.
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Salvi</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>University of North Carolina at Chapel Hill, School of Dentistry, Department of Periodontology, Dental Research Center</s1><s2>Chapel Hill, NC</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region><settlement type="city">Chapel Hill (Caroline du Nord)</settlement></placeName><orgName type="university">Université de Caroline du Nord à Chapel Hill</orgName></affiliation><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>University of Berne, School of Dental Medicine</s1><s2>Berne</s2><s3>CHE</s3><sZ>1 aut.</sZ></inist:fA14><country>Suisse</country><placeName><settlement type="city">Berne</settlement><region type="région" nuts="3">Canton de Berne</region></placeName></affiliation></author><author><name sortKey="Brown, C E" sort="Brown, C E" uniqKey="Brown C" first="C. E." last="Brown">C. E. 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E." last="Salvi">G. E. Salvi</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>University of North Carolina at Chapel Hill, School of Dentistry, Department of Periodontology, Dental Research Center</s1><s2>Chapel Hill, NC</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region><settlement type="city">Chapel Hill (Caroline du Nord)</settlement></placeName><orgName type="university">Université de Caroline du Nord à Chapel Hill</orgName></affiliation><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>University of Berne, School of Dental Medicine</s1><s2>Berne</s2><s3>CHE</s3><sZ>1 aut.</sZ></inist:fA14><country>Suisse</country><placeName><settlement type="city">Berne</settlement><region type="région" nuts="3">Canton de Berne</region></placeName></affiliation></author><author><name sortKey="Brown, C E" sort="Brown, C E" uniqKey="Brown C" first="C. E." last="Brown">C. E. Brown</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>University of North Carolina at Chapel Hill, School of Dentistry, Department of Periodontology, Dental Research Center</s1><s2>Chapel Hill, NC</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region><settlement type="city">Chapel Hill (Caroline du Nord)</settlement></placeName><orgName type="university">Université de Caroline du Nord à Chapel Hill</orgName></affiliation></author><author><name sortKey="Fujihashi, K" sort="Fujihashi, K" uniqKey="Fujihashi K" first="K." last="Fujihashi">K. Fujihashi</name><affiliation wicri:level="2"><inist:fA14 i1="03"><s1>University of Alabama at Birmingham, Department of Oral Biology, Immunobiology Vaccine Center</s1><s2>Birmingham, AL</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Alabama</region></placeName></affiliation></author><author><name sortKey="Kiyono, H" sort="Kiyono, H" uniqKey="Kiyono H" first="H." last="Kiyono">H. Kiyono</name><affiliation wicri:level="2"><inist:fA14 i1="03"><s1>University of Alabama at Birmingham, Department of Oral Biology, Immunobiology Vaccine Center</s1><s2>Birmingham, AL</s2><s3>USA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Alabama</region></placeName></affiliation></author><author><name sortKey="Smith, F W" sort="Smith, F W" uniqKey="Smith F" first="F. W." last="Smith">F. W. Smith</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>University of North Carolina at Chapel Hill, School of Dentistry, Department of Periodontology, Dental Research Center</s1><s2>Chapel Hill, NC</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region><settlement type="city">Chapel Hill (Caroline du Nord)</settlement></placeName><orgName type="university">Université de Caroline du Nord à Chapel Hill</orgName></affiliation></author><author><name sortKey="Becks, J D" sort="Becks, J D" uniqKey="Becks J" first="J. D." last="Becks">J. D. Becks</name><affiliation wicri:level="4"><inist:fA14 i1="04"><s1>University of North Carolina at Chapel Hill, School of Dentistry, Department of Dental Ecology</s1><s2>Chapel Hill, NC</s2><s3>USA</s3><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region><settlement type="city">Chapel Hill (Caroline du Nord)</settlement></placeName><orgName type="university">Université de Caroline du Nord à Chapel Hill</orgName></affiliation></author><author><name sortKey="Offenbacher, S" sort="Offenbacher, S" uniqKey="Offenbacher S" first="S." last="Offenbacher">S. Offenbacher</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>University of North Carolina at Chapel Hill, School of Dentistry, Department of Periodontology, Dental Research Center</s1><s2>Chapel Hill, NC</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Caroline du Nord</region><settlement type="city">Chapel Hill (Caroline du Nord)</settlement></placeName><orgName type="university">Université de Caroline du Nord à Chapel Hill</orgName></affiliation></author></analytic><series><title level="j" type="main">Journal of periodontal research</title><title level="j" type="abbreviated">J. periodontal res.</title><idno type="ISSN">0022-3484</idno><imprint><date when="1998">1998</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of periodontal research</title><title level="j" type="abbreviated">J. periodontal res.</title><idno type="ISSN">0022-3484</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell culture</term><term>Early</term><term>Edentulousness</term><term>Etiopathogenesis</term><term>Helper cell</term><term>Human</term><term>In vitro</term><term>In vivo</term><term>Inflammation</term><term>Interleukin</term><term>Lymphocyte</term><term>Monocyte</term><term>Periodontitis</term><term>Polymerase chain reaction</term><term>Prostaglandin</term><term>Quantitative analysis</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Parodontite</term><term>Edentation</term><term>Précoce</term><term>Analyse quantitative</term><term>Interleukine</term><term>Prostaglandine</term><term>Inflammation</term><term>Monocyte</term><term>Lymphocyte</term><term>Cellule helper</term><term>Réaction chaîne polymérase</term><term>Culture cellulaire</term><term>Etiopathogénie</term><term>Homme</term><term>In vivo</term><term>In vitro</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Analyse quantitative</term><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Based upon the prosthodontic literature, subjects who are at the transition stage between natural dentition and edentulism are called "terminal dentition" (TD) cases. The aim of the present cross-sectional investigation was to characterize the local and systemic inflammatory responses in 2 groups of patients with terminal dentition periodontitis. Eight severe adult periodontitis terminal dentition (AP-TD) subjects and 8 early onset periodontitis terminal dentition (EOP-TD) subjects were entered into the study. Our purpose was to measure an extended battery of cytokines in the gingival crevicular fluid (GCF) and in lipopolysaccharide (LPS)-stimulated monocytic culture supernatants as well as gingival mononuclear cell messenger RNA (mRNA) transcripts determined from biopsy samples. Within the GCF there were 3 tiers (levels) of mediators based upon approximate 10-fold differences in concentration. The highest tier included prostaglandin E<sub>2</sub> (PGE<sub>2</sub>), interleukin-1β (IL-1β) and interleukin-2 (IL-2), the intermediate tier included tumor necrosis factor alpha (TNFα) and interferon gamma (IFN-y) and at the lowest concentration level were interleukin-4 (IL-4) and interleukin-6 (IL-6). Thus, the GCF analysis clearly indicated that in both AP-TD and EOP-TD groups the monocytic, i.e. IL-1 and PGE<sub>2</sub> and Thl, i.e. IL-2 and IFN-y, inflammatory mediator levels quantitatively dominated over the Th2 mediators, i.e. IL-4 and IL-6. LPS-stimulated monocytic release of IL-1β, PGE<sub>2</sub> and TNFα was significantly elevated in both AP-TD and EOP-TD groups compared to those of a control group of 21 subjects with moderate to advanced adult periodontitis. The cytokine mRNA expression of isolated gingival mononuclear cells showed that in both the AP-TD and the EOP-TD groups Thl and Th2 cytokines were expressed, with low levels of IL-4 and IL-12. In conclusion, our data suggest that this cross-sectional TD periodontitis model may reflect progressive periodontal disease associated with tooth loss. Furthermore, although Thl cytokine levels in the GCF dominate over the Th2 response, monocytic activation provides the main source of proinflammatory mediators. In addition, LPS-stimulated peripheral blood monocytes demonstrate an upregulated inflammatory mediator secretion in the terminal dentition.</div></front></TEI><affiliations><list><country><li>Suisse</li><li>États-Unis</li></country><region><li>Alabama</li><li>Canton de Berne</li><li>Caroline du Nord</li></region><settlement><li>Berne</li><li>Chapel Hill (Caroline du Nord)</li></settlement><orgName><li>Université de Caroline du Nord à Chapel Hill</li></orgName></list><tree><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Salvi, G E" sort="Salvi, G E" uniqKey="Salvi G" first="G. E." last="Salvi">G. E. Salvi</name></region><name sortKey="Becks, J D" sort="Becks, J D" uniqKey="Becks J" first="J. D." last="Becks">J. D. Becks</name><name sortKey="Brown, C E" sort="Brown, C E" uniqKey="Brown C" first="C. E." last="Brown">C. E. Brown</name><name sortKey="Fujihashi, K" sort="Fujihashi, K" uniqKey="Fujihashi K" first="K." last="Fujihashi">K. Fujihashi</name><name sortKey="Kiyono, H" sort="Kiyono, H" uniqKey="Kiyono H" first="H." last="Kiyono">H. Kiyono</name><name sortKey="Offenbacher, S" sort="Offenbacher, S" uniqKey="Offenbacher S" first="S." last="Offenbacher">S. Offenbacher</name><name sortKey="Smith, F W" sort="Smith, F W" uniqKey="Smith F" first="F. W." last="Smith">F. W. Smith</name></country><country name="Suisse"><region name="Canton de Berne"><name sortKey="Salvi, G E" sort="Salvi, G E" uniqKey="Salvi G" first="G. E." last="Salvi">G. E. Salvi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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