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Effects of adhesion molecules on the behavior of osteoblast‐like cells and normal human fibroblasts on different titanium surfaces

Identifieur interne : 007671 ( Main/Merge ); précédent : 007670; suivant : 007672

Effects of adhesion molecules on the behavior of osteoblast‐like cells and normal human fibroblasts on different titanium surfaces

Auteurs : Beom Seok Park [Corée du Sud] ; Seong Joo Heo [Corée du Sud] ; Chul Sang Kim [Corée du Sud] ; Ju-Eun Oh [Corée du Sud] ; Jin-Man Kim [Corée du Sud] ; Gene Lee [Corée du Sud] ; Won Ho Park [Corée du Sud] ; Chong-Pyoung Chung [Corée du Sud] ; Byung-Moo Min [Corée du Sud]

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RBID : ISTEX:BA54A984C9723F1C97990920DD90DBFEE93FF59C

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Abstract

This study examined the influences of titanium (Ti) discs with similar surface roughnesses (Ra values), but with different topographies and chemical compositions, on the adhesion, spreading, and the alkaline phosphatase (ALP) activity of osteoblast‐like cells and normal human fibroblasts. The presence of adhesion molecules on the Ti surfaces and their effects on cell activity were also investigated. Two types of Ti discs were prepared. One kind was a mechanically polished Ti disc, and the other type was a disc obtained by the heating of hydroxyapatite (HA) dip‐coated Ti. Scanning electron microscopy, optical interferometry, and scanning Auger electron spectroscopy were used to examine the surface morphology, roughness, and chemical composition, respectively, of the superficial Ti layer. The two types of Ti discs had different topographies and chemical compositions, but had similar Ra values. The cells on both surface types had similar behaviors and ALP activities. A biological evaluation of the surface‐modified Ti discs showed that the type I collagen coating was functionally active in terms of cell spreading in both types of Ti discs. In the mechanically polished Ti discs, fibronectin was functionally active in the normal human fibroblasts, but not in the osteoblast‐like cells. Cell adhesion was slightly better on the heat‐treated HA dip‐coated Ti discs, but not on the mechanically polished Ti discs. Type I collagen and fibronectin mediated the adhesion and spreading of osteoblast‐like cells through α2β1 integrin and α5β1 integrin, respectively. These results suggest that type I collagen might be a good candidate for the biochemical modification of Ti surfaces, particularly those surfaces obtained by heating of HA dip‐coated Ti. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005

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DOI: 10.1002/jbm.a.30326

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<term>Absolute values</term>
<term>Adherent cells</term>
<term>Adhesion</term>
<term>Adhesion molecules</term>
<term>Adhesion titanium surfaces</term>
<term>Adsorption</term>
<term>Alkaline phosphatase</term>
<term>Assay</term>
<term>Auger electron transitions</term>
<term>Biol chem</term>
<term>Biomaterials</term>
<term>Biomaterials science</term>
<term>Biomed</term>
<term>Biomed mater</term>
<term>Bone cells</term>
<term>Broblasts</term>
<term>Bronectin</term>
<term>Bronectin conformation</term>
<term>Cacodylate buffer</term>
<term>Calcium phosphate</term>
<term>Calvarial osteoblasts</term>
<term>Cell adhesion</term>
<term>Cell attachment</term>
<term>Cell behaviors</term>
<term>Cell growth</term>
<term>Cell suspension</term>
<term>Cellular response</term>
<term>Chemical composition</term>
<term>Chemical compositions</term>
<term>Collagen</term>
<term>Collagen coating</term>
<term>Collagen immobilization</term>
<term>Compositional variations</term>
<term>Control plastic dishes</term>
<term>Crystal violet</term>
<term>Culture medium</term>
<term>Culture plate</term>
<term>Culture plates</term>
<term>Dental implants</term>
<term>Dental research institute</term>
<term>Different surfaces</term>
<term>Different topographies</term>
<term>Different types</term>
<term>Disc</term>
<term>Disc surfaces</term>
<term>Disc types</term>
<term>Extracellular matrix</term>
<term>Good candidate</term>
<term>Heat treatment</term>
<term>Human plasma</term>
<term>Implant</term>
<term>Implant material</term>
<term>Implant materials</term>
<term>Implant surface</term>
<term>Implant surfaces</term>
<term>Important role</term>
<term>Independent experiments</term>
<term>Inhibition assay</term>
<term>Initial behavior</term>
<term>Integrin</term>
<term>Integrin binding</term>
<term>Integrin interaction</term>
<term>Integrin subunits</term>
<term>Integrins</term>
<term>Kinetic energies</term>
<term>Mater</term>
<term>Matrix</term>
<term>Matrix molecules</term>
<term>Monoclonal antibodies</term>
<term>Nhgf</term>
<term>Nonspreading cells</term>
<term>Optical interferometer</term>
<term>Oral maxillofac implants</term>
<term>Osteoblast</term>
<term>Osteoblast phenotype</term>
<term>Osteoblast responses</term>
<term>Osteoblastic</term>
<term>Osteoblastic cells</term>
<term>Osteoblastic differentiation</term>
<term>Osteoblastlike cells</term>
<term>Other type</term>
<term>Overnight adsorption</term>
<term>Pairwise comparisons</term>
<term>Phase transition temperature</term>
<term>Plate surface</term>
<term>Polygonal shape</term>
<term>Polystyrene</term>
<term>Polystyrene surface</term>
<term>Protective argon atmosphere</term>
<term>Receptor</term>
<term>Representative count</term>
<term>Rinsed</term>
<term>Saes depth</term>
<term>Scanning auger electron spectroscopy</term>
<term>Scanning electron microscopy</term>
<term>Seoul</term>
<term>Seoul korea</term>
<term>Subunit</term>
<term>Surface departures</term>
<term>Surface engineering</term>
<term>Surface roughness</term>
<term>Surface topography</term>
<term>Titanium</term>
<term>Titanium implants</term>
<term>Unattached cells</term>
<term>Uncoated</term>
<term>Uncoated counterparts</term>
<term>Uncoated surfaces</term>
<term>Wiley periodicals</term>
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<term>Absolute values</term>
<term>Adherent cells</term>
<term>Adhesion</term>
<term>Adhesion molecules</term>
<term>Adhesion titanium surfaces</term>
<term>Adsorption</term>
<term>Alkaline phosphatase</term>
<term>Assay</term>
<term>Auger electron transitions</term>
<term>Biol chem</term>
<term>Biomaterials</term>
<term>Biomaterials science</term>
<term>Biomed</term>
<term>Biomed mater</term>
<term>Bone cells</term>
<term>Broblasts</term>
<term>Bronectin</term>
<term>Bronectin conformation</term>
<term>Cacodylate buffer</term>
<term>Calcium phosphate</term>
<term>Calvarial osteoblasts</term>
<term>Cell adhesion</term>
<term>Cell attachment</term>
<term>Cell behaviors</term>
<term>Cell growth</term>
<term>Cell suspension</term>
<term>Cellular response</term>
<term>Chemical composition</term>
<term>Chemical compositions</term>
<term>Collagen</term>
<term>Collagen coating</term>
<term>Collagen immobilization</term>
<term>Compositional variations</term>
<term>Control plastic dishes</term>
<term>Crystal violet</term>
<term>Culture medium</term>
<term>Culture plate</term>
<term>Culture plates</term>
<term>Dental implants</term>
<term>Dental research institute</term>
<term>Different surfaces</term>
<term>Different topographies</term>
<term>Different types</term>
<term>Disc</term>
<term>Disc surfaces</term>
<term>Disc types</term>
<term>Extracellular matrix</term>
<term>Good candidate</term>
<term>Heat treatment</term>
<term>Human plasma</term>
<term>Implant</term>
<term>Implant material</term>
<term>Implant materials</term>
<term>Implant surface</term>
<term>Implant surfaces</term>
<term>Important role</term>
<term>Independent experiments</term>
<term>Inhibition assay</term>
<term>Initial behavior</term>
<term>Integrin</term>
<term>Integrin binding</term>
<term>Integrin interaction</term>
<term>Integrin subunits</term>
<term>Integrins</term>
<term>Kinetic energies</term>
<term>Mater</term>
<term>Matrix</term>
<term>Matrix molecules</term>
<term>Monoclonal antibodies</term>
<term>Nhgf</term>
<term>Nonspreading cells</term>
<term>Optical interferometer</term>
<term>Oral maxillofac implants</term>
<term>Osteoblast</term>
<term>Osteoblast phenotype</term>
<term>Osteoblast responses</term>
<term>Osteoblastic</term>
<term>Osteoblastic cells</term>
<term>Osteoblastic differentiation</term>
<term>Osteoblastlike cells</term>
<term>Other type</term>
<term>Overnight adsorption</term>
<term>Pairwise comparisons</term>
<term>Phase transition temperature</term>
<term>Plate surface</term>
<term>Polygonal shape</term>
<term>Polystyrene</term>
<term>Polystyrene surface</term>
<term>Protective argon atmosphere</term>
<term>Receptor</term>
<term>Representative count</term>
<term>Rinsed</term>
<term>Saes depth</term>
<term>Scanning auger electron spectroscopy</term>
<term>Scanning electron microscopy</term>
<term>Seoul</term>
<term>Seoul korea</term>
<term>Subunit</term>
<term>Surface departures</term>
<term>Surface engineering</term>
<term>Surface roughness</term>
<term>Surface topography</term>
<term>Titanium</term>
<term>Titanium implants</term>
<term>Unattached cells</term>
<term>Uncoated</term>
<term>Uncoated counterparts</term>
<term>Uncoated surfaces</term>
<term>Wiley periodicals</term>
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<term>Biomatériau</term>
<term>Titane</term>
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<front>
<div type="abstract" xml:lang="en">This study examined the influences of titanium (Ti) discs with similar surface roughnesses (Ra values), but with different topographies and chemical compositions, on the adhesion, spreading, and the alkaline phosphatase (ALP) activity of osteoblast‐like cells and normal human fibroblasts. The presence of adhesion molecules on the Ti surfaces and their effects on cell activity were also investigated. Two types of Ti discs were prepared. One kind was a mechanically polished Ti disc, and the other type was a disc obtained by the heating of hydroxyapatite (HA) dip‐coated Ti. Scanning electron microscopy, optical interferometry, and scanning Auger electron spectroscopy were used to examine the surface morphology, roughness, and chemical composition, respectively, of the superficial Ti layer. The two types of Ti discs had different topographies and chemical compositions, but had similar Ra values. The cells on both surface types had similar behaviors and ALP activities. A biological evaluation of the surface‐modified Ti discs showed that the type I collagen coating was functionally active in terms of cell spreading in both types of Ti discs. In the mechanically polished Ti discs, fibronectin was functionally active in the normal human fibroblasts, but not in the osteoblast‐like cells. Cell adhesion was slightly better on the heat‐treated HA dip‐coated Ti discs, but not on the mechanically polished Ti discs. Type I collagen and fibronectin mediated the adhesion and spreading of osteoblast‐like cells through α2β1 integrin and α5β1 integrin, respectively. These results suggest that type I collagen might be a good candidate for the biochemical modification of Ti surfaces, particularly those surfaces obtained by heating of HA dip‐coated Ti. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005</div>
</front>
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   |texte=   Effects of adhesion molecules on the behavior of osteoblast‐like cells and normal human fibroblasts on different titanium surfaces
}}

Wicri

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