Comparison of fibronectin in human marginal gingiva and interdental papilla using immunohistochemistry
Identifieur interne : 004282 ( Main/Merge ); précédent : 004281; suivant : 004283Comparison of fibronectin in human marginal gingiva and interdental papilla using immunohistochemistry
Auteurs : Mohammad Tavakoli [Iran] ; Ensiyeh Bateni [Iran] ; Fazeleh Attarbashi-Moghadam [Iran] ; Ardeshir Talebi [Iran] ; Jaber Yaghini [Iran] ; Ahmad Mogharehabed [Iran]Source :
- Dental Research Journal [ 1735-3327 ] ; 2011.
Abstract
The differences between marginal gingiva and interdental papilla may be due to variation in the molecular composition of these two different anatomical structures. The aim of this study was to evaluate the staining intensity of fibronectin in human marginal gingiva and interdental papilla.
In a prospective analytical study, 16 healthy subjects needing crown lengthening surgery were selected. All participants were medically healthy, non-smokers, with no medication intake, and a healthy periodontium. During surgery, facial/buccal marginal gingiva and interdental papilla were separately harvested. The specimens were subjected to hematoxylin and eosin, histochemical (Masson’ strichorom, reticulin, and elastic), and immunohistochemical staining for evaluation of morphology and inflammation; assessment of connective tissue fibers (collagen, reticulin, and elastic); and determination of fibronectin staining intensity. The data were analyzed by Spsssoftware, Wilcoxon, and Spearman tests.
From a total of 32 specimens, 21 specimens were found to be normal or having mild inflammation, while the remaining specimens had moderate to severe inflammation in some parts. Collagen fibers were found to be dense in reticular connective tissue and degenerated in the region of inflammation. Reticulin fibers strongly stained near epithelium. Elastic fibers were sparsely found. Mean fibronectin staining intensity between marginal gingiva and interdental papilla was not statically significant (
This study did not show any difference in interdental papilla and marginal gingival with respect to fibronectin composition. More studies in this context are needed.
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PubMed: 23372588
PubMed Central: 3556296
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Center, Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Torabinejad Dental Research Center, Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan</wicri:regionArea><wicri:noRegion>Isfahan</wicri:noRegion></affiliation></author><author><name sortKey="Attarbashi Moghadam, Fazeleh" sort="Attarbashi Moghadam, Fazeleh" uniqKey="Attarbashi Moghadam F" first="Fazeleh" last="Attarbashi-Moghadam">Fazeleh Attarbashi-Moghadam</name><affiliation wicri:level="1"><nlm:aff id="aff1">Torabinejad Dental Research Center, Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Torabinejad Dental Research Center, Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, 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interdental papilla using immunohistochemistry</title><author><name sortKey="Tavakoli, Mohammad" sort="Tavakoli, Mohammad" uniqKey="Tavakoli M" first="Mohammad" last="Tavakoli">Mohammad Tavakoli</name><affiliation wicri:level="1"><nlm:aff id="aff1">Torabinejad Dental Research Center, Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Torabinejad Dental Research Center, Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan</wicri:regionArea><wicri:noRegion>Isfahan</wicri:noRegion></affiliation></author><author><name sortKey="Bateni, Ensiyeh" sort="Bateni, Ensiyeh" uniqKey="Bateni E" first="Ensiyeh" last="Bateni">Ensiyeh Bateni</name><affiliation wicri:level="1"><nlm:aff id="aff1">Torabinejad Dental Research Center, Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Torabinejad Dental Research Center, Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan</wicri:regionArea><wicri:noRegion>Isfahan</wicri:noRegion></affiliation></author><author><name sortKey="Attarbashi Moghadam, Fazeleh" sort="Attarbashi Moghadam, Fazeleh" uniqKey="Attarbashi Moghadam F" first="Fazeleh" last="Attarbashi-Moghadam">Fazeleh Attarbashi-Moghadam</name><affiliation wicri:level="1"><nlm:aff id="aff1">Torabinejad Dental Research Center, Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Torabinejad Dental Research Center, Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan</wicri:regionArea><wicri:noRegion>Isfahan</wicri:noRegion></affiliation></author><author><name sortKey="Talebi, Ardeshir" sort="Talebi, Ardeshir" uniqKey="Talebi A" first="Ardeshir" last="Talebi">Ardeshir Talebi</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan</wicri:regionArea><wicri:noRegion>Isfahan</wicri:noRegion></affiliation></author><author><name sortKey="Yaghini, Jaber" sort="Yaghini, Jaber" uniqKey="Yaghini J" first="Jaber" last="Yaghini">Jaber Yaghini</name><affiliation wicri:level="1"><nlm:aff id="aff1">Torabinejad Dental Research Center, Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Torabinejad Dental Research Center, Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan</wicri:regionArea><wicri:noRegion>Isfahan</wicri:noRegion></affiliation></author><author><name sortKey="Mogharehabed, Ahmad" sort="Mogharehabed, Ahmad" uniqKey="Mogharehabed A" first="Ahmad" last="Mogharehabed">Ahmad Mogharehabed</name><affiliation wicri:level="1"><nlm:aff id="aff3">Department of Periodontology, Faculty of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran</nlm:aff><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Periodontology, Faculty of Dentistry, Isfahan University of Medical Sciences, Isfahan</wicri:regionArea><wicri:noRegion>Isfahan</wicri:noRegion></affiliation></author></analytic><series><title level="j">Dental Research Journal</title><idno type="ISSN">1735-3327</idno><idno type="eISSN">2008-0255</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="st1"><title>Background:</title><p>The differences between marginal gingiva and interdental papilla may be due to variation in the molecular composition of these two different anatomical structures. The aim of this study was to evaluate the staining intensity of fibronectin in human marginal gingiva and interdental papilla.</p></sec><sec id="st2"><title>Materials and Methods:</title><p>In a prospective analytical study, 16 healthy subjects needing crown lengthening surgery were selected. All participants were medically healthy, non-smokers, with no medication intake, and a healthy periodontium. During surgery, facial/buccal marginal gingiva and interdental papilla were separately harvested. The specimens were subjected to hematoxylin and eosin, histochemical (Masson’ strichorom, reticulin, and elastic), and immunohistochemical staining for evaluation of morphology and inflammation; assessment of connective tissue fibers (collagen, reticulin, and elastic); and determination of fibronectin staining intensity. The data were analyzed by Spsssoftware, Wilcoxon, and Spearman tests. <italic>P</italic><0.05 was considered to be statistically significant.</p></sec><sec id="st3"><title>Results:</title><p>From a total of 32 specimens, 21 specimens were found to be normal or having mild inflammation, while the remaining specimens had moderate to severe inflammation in some parts. Collagen fibers were found to be dense in reticular connective tissue and degenerated in the region of inflammation. Reticulin fibers strongly stained near epithelium. Elastic fibers were sparsely found. Mean fibronectin staining intensity between marginal gingiva and interdental papilla was not statically significant (<italic>P</italic>=0.44). There is no statistically significant correlation between tissue inflammation and fibronectin staining intensity (<italic>P</italic>=0.76 for marginal gingival and <italic>P</italic>=0.20 for interdental papilla). Considering all specimens, fibronectin staining intensity of connective tissue adjacent to Sulcular/Junctional epithelium was higher than reticular connective tissue (<italic>P</italic>=0.003) and higher than connective tissue adjacent to oral epithelium (<italic>P</italic><0.001).</p></sec><sec id="st4"><title>Conclusion:</title><p>This study did not show any difference in interdental papilla and marginal gingival with respect to fibronectin composition. 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