Influence of topography and hydrophilicity on initial oral biofilm formation on microstructured titanium surfaces in vitro
Identifieur interne : 003A35 ( Main/Merge ); précédent : 003A34; suivant : 003A36Influence of topography and hydrophilicity on initial oral biofilm formation on microstructured titanium surfaces in vitro
Auteurs : A. Almaguer-Flores [Mexique] ; R. Olivares-Navarrete [États-Unis] ; M. Wieland [Suisse] ; L. A. Ximénez-Fyvie [Mexique] ; Z. Schwartz [États-Unis] ; B. D. Boyan [États-Unis]Source :
- Clinical Oral Implants Research [ 0905-7161 ] ; 2012-03.
Descripteurs français
- Wicri :
- topic : Titane.
English descriptors
- KwdEn :
- Actinomyces israelii, Actinomycetemcomitans, Adhesion, American type culture collection, Anaerobic conditions, Anaerobic incubation, Analytical biochemistry, Bacterial adhesion, Bacterial counts, Bacterial species, Bacterial strains, Biomedical, Biomedical materials research, Biomedical materials research part, Biorad laboratories, Boyan, Chemical properties, Clin, Clinical periodontology, Colonization, Concise review, Culture medium, Dental implant, Dental research, Eikenella corrodens, Extracellular matrix, Fusobacterium nucleatum, Gingivalis, High proportions, Higher numbers, Human saliva, Hybridization, Hybridization solution, Hydrophilic, Hydrophilicity, Impl, Implant, Implant surfaces, Implants research, Incubation media, Initial formation, Institut straumann, Israelii, John wiley sons, Katsikogianni missirlis, Lowest proportion, Microstructured, Microstructured titanium, Microstructured titanium surfaces, Microtopography, Moda, Moda surfaces, Modsla, Modsla substrates, Modsla surfaces, More bacteria, Mycoplasma broth, Nucleatum, Oral cavity, Oral impl, Oral microbiology, Parvimonas micra, Periodontal, Periodontology, Pilot study, Plaque, Porphyromonas gingivalis, Prevotella intermedia, Quirynen, Reference strain, Reference strains, Roche diagnostics, Room temperature, Saliva, Sanguinis, Scanning electron micrographs, Socransky, Streptococcus sanguinis, Subgingival, Subgingival plaque, Surface energy, Surface microtopography, Surface morphology, Surface properties, Surface roughness, Surface topography, Test substrates, Titanium, Titanium surface, Total number, Universidad nacional autonoma, Vivo study, Wieland.
- Teeft :
- Actinomyces israelii, Actinomycetemcomitans, Adhesion, American type culture collection, Anaerobic conditions, Anaerobic incubation, Analytical biochemistry, Bacterial adhesion, Bacterial counts, Bacterial species, Bacterial strains, Biomedical, Biomedical materials research, Biomedical materials research part, Biorad laboratories, Boyan, Chemical properties, Clin, Clinical periodontology, Colonization, Concise review, Culture medium, Dental implant, Dental research, Eikenella corrodens, Extracellular matrix, Fusobacterium nucleatum, Gingivalis, High proportions, Higher numbers, Human saliva, Hybridization, Hybridization solution, Hydrophilic, Hydrophilicity, Impl, Implant, Implant surfaces, Implants research, Incubation media, Initial formation, Institut straumann, Israelii, John wiley sons, Katsikogianni missirlis, Lowest proportion, Microstructured, Microstructured titanium, Microstructured titanium surfaces, Microtopography, Moda, Moda surfaces, Modsla, Modsla substrates, Modsla surfaces, More bacteria, Mycoplasma broth, Nucleatum, Oral cavity, Oral impl, Oral microbiology, Parvimonas micra, Periodontal, Periodontology, Pilot study, Plaque, Porphyromonas gingivalis, Prevotella intermedia, Quirynen, Reference strain, Reference strains, Roche diagnostics, Room temperature, Saliva, Sanguinis, Scanning electron micrographs, Socransky, Streptococcus sanguinis, Subgingival, Subgingival plaque, Surface energy, Surface microtopography, Surface morphology, Surface properties, Surface roughness, Surface topography, Test substrates, Titanium, Titanium surface, Total number, Universidad nacional autonoma, Vivo study, Wieland.
Abstract
Objectives: The aim of this study was to analyse the influence of the microtopography and hydrophilicity of titanium (Ti) substrates on initial oral biofilm formation.
Url:
- https://api.istex.fr/document/4D395FE06830C42DEF39804B27F8CEB5B62AF6E6/fulltext/pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287405
DOI: 10.1111/j.1600-0501.2011.02184.x
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ISTEX:4D395FE06830C42DEF39804B27F8CEB5B62AF6E6Le document en format XML
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proportions</term><term>Higher numbers</term><term>Human saliva</term><term>Hybridization</term><term>Hybridization solution</term><term>Hydrophilic</term><term>Hydrophilicity</term><term>Impl</term><term>Implant</term><term>Implant surfaces</term><term>Implants research</term><term>Incubation media</term><term>Initial formation</term><term>Institut straumann</term><term>Israelii</term><term>John wiley sons</term><term>Katsikogianni missirlis</term><term>Lowest proportion</term><term>Microstructured</term><term>Microstructured titanium</term><term>Microstructured titanium surfaces</term><term>Microtopography</term><term>Moda</term><term>Moda surfaces</term><term>Modsla</term><term>Modsla substrates</term><term>Modsla surfaces</term><term>More bacteria</term><term>Mycoplasma broth</term><term>Nucleatum</term><term>Oral cavity</term><term>Oral impl</term><term>Oral microbiology</term><term>Parvimonas micra</term><term>Periodontal</term><term>Periodontology</term><term>Pilot study</term><term>Plaque</term><term>Porphyromonas gingivalis</term><term>Prevotella intermedia</term><term>Quirynen</term><term>Reference strain</term><term>Reference strains</term><term>Roche diagnostics</term><term>Room temperature</term><term>Saliva</term><term>Sanguinis</term><term>Scanning electron micrographs</term><term>Socransky</term><term>Streptococcus sanguinis</term><term>Subgingival</term><term>Subgingival plaque</term><term>Surface energy</term><term>Surface microtopography</term><term>Surface morphology</term><term>Surface properties</term><term>Surface roughness</term><term>Surface topography</term><term>Test substrates</term><term>Titanium</term><term>Titanium surface</term><term>Total number</term><term>Universidad nacional autonoma</term><term>Vivo study</term><term>Wieland</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Actinomyces israelii</term><term>Actinomycetemcomitans</term><term>Adhesion</term><term>American type culture collection</term><term>Anaerobic conditions</term><term>Anaerobic incubation</term><term>Analytical biochemistry</term><term>Bacterial adhesion</term><term>Bacterial counts</term><term>Bacterial species</term><term>Bacterial strains</term><term>Biomedical</term><term>Biomedical materials research</term><term>Biomedical materials research part</term><term>Biorad laboratories</term><term>Boyan</term><term>Chemical properties</term><term>Clin</term><term>Clinical periodontology</term><term>Colonization</term><term>Concise review</term><term>Culture medium</term><term>Dental implant</term><term>Dental research</term><term>Eikenella corrodens</term><term>Extracellular matrix</term><term>Fusobacterium nucleatum</term><term>Gingivalis</term><term>High proportions</term><term>Higher numbers</term><term>Human saliva</term><term>Hybridization</term><term>Hybridization solution</term><term>Hydrophilic</term><term>Hydrophilicity</term><term>Impl</term><term>Implant</term><term>Implant surfaces</term><term>Implants research</term><term>Incubation media</term><term>Initial formation</term><term>Institut straumann</term><term>Israelii</term><term>John wiley sons</term><term>Katsikogianni missirlis</term><term>Lowest proportion</term><term>Microstructured</term><term>Microstructured titanium</term><term>Microstructured titanium surfaces</term><term>Microtopography</term><term>Moda</term><term>Moda surfaces</term><term>Modsla</term><term>Modsla substrates</term><term>Modsla surfaces</term><term>More bacteria</term><term>Mycoplasma broth</term><term>Nucleatum</term><term>Oral cavity</term><term>Oral impl</term><term>Oral microbiology</term><term>Parvimonas micra</term><term>Periodontal</term><term>Periodontology</term><term>Pilot study</term><term>Plaque</term><term>Porphyromonas gingivalis</term><term>Prevotella intermedia</term><term>Quirynen</term><term>Reference strain</term><term>Reference strains</term><term>Roche diagnostics</term><term>Room temperature</term><term>Saliva</term><term>Sanguinis</term><term>Scanning electron micrographs</term><term>Socransky</term><term>Streptococcus sanguinis</term><term>Subgingival</term><term>Subgingival plaque</term><term>Surface energy</term><term>Surface microtopography</term><term>Surface morphology</term><term>Surface properties</term><term>Surface roughness</term><term>Surface topography</term><term>Test substrates</term><term>Titanium</term><term>Titanium surface</term><term>Total number</term><term>Universidad nacional autonoma</term><term>Vivo study</term><term>Wieland</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Titane</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">Objectives: The aim of this study was to analyse the influence of the microtopography and hydrophilicity of titanium (Ti) substrates on initial oral biofilm formation.</div></front></TEI><double doi="10.1111/j.1600-0501.2011.02184.x"><ISTEX><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Influence of topography and hydrophilicity on initial oral biofilm formation on microstructured titanium surfaces in vitro</title><author><name sortKey="Almaguer Lores, A" sort="Almaguer Lores, A" uniqKey="Almaguer Lores A" first="A." last="Almaguer-Flores">A. Almaguer-Flores</name></author><author><name sortKey="Olivares Avarrete, R" sort="Olivares Avarrete, R" uniqKey="Olivares Avarrete R" first="R." last="Olivares-Navarrete">R. Olivares-Navarrete</name></author><author><name sortKey="Wieland, M" sort="Wieland, M" uniqKey="Wieland M" first="M." last="Wieland">M. Wieland</name></author><author><name sortKey="Ximenez Yvie, L A" sort="Ximenez Yvie, L A" uniqKey="Ximenez Yvie L" first="L. A." last="Ximénez-Fyvie">L. A. Ximénez-Fyvie</name></author><author><name sortKey="Schwartz, Z" sort="Schwartz, Z" uniqKey="Schwartz Z" first="Z." last="Schwartz">Z. Schwartz</name></author><author><name sortKey="Boyan, B D" sort="Boyan, B D" uniqKey="Boyan B" first="B. D." last="Boyan">B. D. Boyan</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:4D395FE06830C42DEF39804B27F8CEB5B62AF6E6</idno><date when="2012" year="2012">2012</date><idno type="doi">10.1111/j.1600-0501.2011.02184.x</idno><idno type="url">https://api.istex.fr/document/4D395FE06830C42DEF39804B27F8CEB5B62AF6E6/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">002595</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002595</idno><idno type="wicri:Area/Istex/Curation">002595</idno><idno type="wicri:Area/Istex/Checkpoint">000E88</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000E88</idno><idno type="wicri:doubleKey">0905-7161:2012:Almaguer Lores A:influence:of:topography</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Influence of topography and hydrophilicity on initial oral biofilm formation on microstructured titanium surfaces <hi rend="italic">in vitro</hi></title><author><name sortKey="Almaguer Lores, A" sort="Almaguer Lores, A" uniqKey="Almaguer Lores A" first="A." last="Almaguer-Flores">A. Almaguer-Flores</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Mexique</country><wicri:regionArea>Instituto de Investigaciones en Materiales, Universidad Nacional, Autónoma de México, Ciudad Universitaria, México D. F.</wicri:regionArea><wicri:noRegion>México D. F.</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Mexique</country><wicri:regionArea>Laboratorio de Genética Molecular, Facultad de Odontología, Universidad Nacional Autónoma de México, México D. F.</wicri:regionArea><wicri:noRegion>México D. F.</wicri:noRegion></affiliation></author><author><name sortKey="Olivares Avarrete, R" sort="Olivares Avarrete, R" uniqKey="Olivares Avarrete R" first="R." last="Olivares-Navarrete">R. Olivares-Navarrete</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Wieland, M" sort="Wieland, M" uniqKey="Wieland M" first="M." last="Wieland">M. Wieland</name><affiliation wicri:level="3"><country xml:lang="fr">Suisse</country><wicri:regionArea>MyoPowers Medical Technologies SA, Lausanne</wicri:regionArea><placeName><settlement type="city">Lausanne</settlement><region nuts="3" type="region">Canton de Vaud</region></placeName></affiliation></author><author><name sortKey="Ximenez Yvie, L A" sort="Ximenez Yvie, L A" uniqKey="Ximenez Yvie L" first="L. A." last="Ximénez-Fyvie">L. A. Ximénez-Fyvie</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Mexique</country><wicri:regionArea>Laboratorio de Genética Molecular, Facultad de Odontología, Universidad Nacional Autónoma de México, México D. F.</wicri:regionArea><wicri:noRegion>México D. F.</wicri:noRegion></affiliation></author><author><name sortKey="Schwartz, Z" sort="Schwartz, Z" uniqKey="Schwartz Z" first="Z." last="Schwartz">Z. Schwartz</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Boyan, B D" sort="Boyan, B D" uniqKey="Boyan B" first="B. D." last="Boyan">B. D. Boyan</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Clinical Oral Implants Research</title><title level="j" type="alt">CLINICAL ORAL IMPLANTS RESEARCH</title><idno type="ISSN">0905-7161</idno><idno type="eISSN">1600-0501</idno><imprint><biblScope unit="vol">23</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="301">301</biblScope><biblScope unit="page" to="307">307</biblScope><biblScope unit="page-count">7</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2012-03">2012-03</date></imprint><idno type="ISSN">0905-7161</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0905-7161</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actinomyces israelii</term><term>Actinomycetemcomitans</term><term>Adhesion</term><term>American type culture collection</term><term>Anaerobic conditions</term><term>Anaerobic incubation</term><term>Analytical biochemistry</term><term>Bacterial adhesion</term><term>Bacterial counts</term><term>Bacterial species</term><term>Bacterial strains</term><term>Biomedical</term><term>Biomedical materials research</term><term>Biomedical materials research part</term><term>Biorad laboratories</term><term>Boyan</term><term>Chemical properties</term><term>Clin</term><term>Clinical periodontology</term><term>Colonization</term><term>Concise review</term><term>Culture medium</term><term>Dental implant</term><term>Dental research</term><term>Eikenella corrodens</term><term>Extracellular matrix</term><term>Fusobacterium nucleatum</term><term>Gingivalis</term><term>High proportions</term><term>Higher numbers</term><term>Human saliva</term><term>Hybridization</term><term>Hybridization solution</term><term>Hydrophilic</term><term>Hydrophilicity</term><term>Impl</term><term>Implant</term><term>Implant surfaces</term><term>Implants research</term><term>Incubation media</term><term>Initial formation</term><term>Institut straumann</term><term>Israelii</term><term>John wiley sons</term><term>Katsikogianni missirlis</term><term>Lowest proportion</term><term>Microstructured</term><term>Microstructured titanium</term><term>Microstructured titanium surfaces</term><term>Microtopography</term><term>Moda</term><term>Moda surfaces</term><term>Modsla</term><term>Modsla substrates</term><term>Modsla surfaces</term><term>More bacteria</term><term>Mycoplasma broth</term><term>Nucleatum</term><term>Oral cavity</term><term>Oral impl</term><term>Oral microbiology</term><term>Parvimonas micra</term><term>Periodontal</term><term>Periodontology</term><term>Pilot study</term><term>Plaque</term><term>Porphyromonas gingivalis</term><term>Prevotella intermedia</term><term>Quirynen</term><term>Reference strain</term><term>Reference strains</term><term>Roche diagnostics</term><term>Room temperature</term><term>Saliva</term><term>Sanguinis</term><term>Scanning electron micrographs</term><term>Socransky</term><term>Streptococcus sanguinis</term><term>Subgingival</term><term>Subgingival plaque</term><term>Surface energy</term><term>Surface microtopography</term><term>Surface morphology</term><term>Surface properties</term><term>Surface roughness</term><term>Surface topography</term><term>Test substrates</term><term>Titanium</term><term>Titanium surface</term><term>Total number</term><term>Universidad nacional autonoma</term><term>Vivo study</term><term>Wieland</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Actinomyces israelii</term><term>Actinomycetemcomitans</term><term>Adhesion</term><term>American type culture collection</term><term>Anaerobic conditions</term><term>Anaerobic incubation</term><term>Analytical biochemistry</term><term>Bacterial adhesion</term><term>Bacterial counts</term><term>Bacterial species</term><term>Bacterial strains</term><term>Biomedical</term><term>Biomedical materials research</term><term>Biomedical materials research part</term><term>Biorad laboratories</term><term>Boyan</term><term>Chemical properties</term><term>Clin</term><term>Clinical periodontology</term><term>Colonization</term><term>Concise review</term><term>Culture medium</term><term>Dental implant</term><term>Dental research</term><term>Eikenella corrodens</term><term>Extracellular matrix</term><term>Fusobacterium nucleatum</term><term>Gingivalis</term><term>High proportions</term><term>Higher numbers</term><term>Human saliva</term><term>Hybridization</term><term>Hybridization solution</term><term>Hydrophilic</term><term>Hydrophilicity</term><term>Impl</term><term>Implant</term><term>Implant surfaces</term><term>Implants research</term><term>Incubation media</term><term>Initial formation</term><term>Institut straumann</term><term>Israelii</term><term>John wiley sons</term><term>Katsikogianni missirlis</term><term>Lowest proportion</term><term>Microstructured</term><term>Microstructured titanium</term><term>Microstructured titanium surfaces</term><term>Microtopography</term><term>Moda</term><term>Moda surfaces</term><term>Modsla</term><term>Modsla substrates</term><term>Modsla surfaces</term><term>More bacteria</term><term>Mycoplasma broth</term><term>Nucleatum</term><term>Oral cavity</term><term>Oral impl</term><term>Oral microbiology</term><term>Parvimonas micra</term><term>Periodontal</term><term>Periodontology</term><term>Pilot study</term><term>Plaque</term><term>Porphyromonas gingivalis</term><term>Prevotella intermedia</term><term>Quirynen</term><term>Reference strain</term><term>Reference strains</term><term>Roche diagnostics</term><term>Room temperature</term><term>Saliva</term><term>Sanguinis</term><term>Scanning electron micrographs</term><term>Socransky</term><term>Streptococcus sanguinis</term><term>Subgingival</term><term>Subgingival plaque</term><term>Surface energy</term><term>Surface microtopography</term><term>Surface morphology</term><term>Surface properties</term><term>Surface roughness</term><term>Surface topography</term><term>Test substrates</term><term>Titanium</term><term>Titanium surface</term><term>Total number</term><term>Universidad nacional autonoma</term><term>Vivo study</term><term>Wieland</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Titane</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">Objectives: The aim of this study was to analyse the influence of the microtopography and hydrophilicity of titanium (Ti) substrates on initial oral biofilm formation.</div></front></TEI></ISTEX><PMC><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Influence of topography and hydrophilicity on initial oral biofilm formation on microstructured titanium surfaces <italic>in vitro</italic></title><author><name sortKey="Almaguer Flores, A" sort="Almaguer Flores, A" uniqKey="Almaguer Flores A" first="A." last="Almaguer-Flores">A. Almaguer-Flores</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21492236</idno><idno type="pmc">4287405</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287405</idno><idno type="RBID">PMC:4287405</idno><idno type="doi">10.1111/j.1600-0501.2011.02184.x</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">002761</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002761</idno><idno type="wicri:Area/Pmc/Curation">002761</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002761</idno><idno type="wicri:Area/Pmc/Checkpoint">002152</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002152</idno><idno type="wicri:Area/Ncbi/Merge">003576</idno><idno type="wicri:Area/Ncbi/Curation">003576</idno><idno type="wicri:Area/Ncbi/Checkpoint">003576</idno><idno type="wicri:doubleKey">0905-7161:2011:Almaguer Flores A:influence:of:topography</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Influence of topography and hydrophilicity on initial oral biofilm formation on microstructured titanium surfaces <italic>in vitro</italic></title><author><name sortKey="Almaguer Flores, A" sort="Almaguer Flores, A" uniqKey="Almaguer Flores A" first="A." last="Almaguer-Flores">A. Almaguer-Flores</name></author></analytic><series><title level="j">Clinical oral implants research</title><idno type="ISSN">0905-7161</idno><idno type="eISSN">1600-0501</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="S1"><title>Objectives</title><p id="P1">The aim of this study was to analyse the influence of the microtopography and hydrophilicity of titanium (Ti) substrates on initial oral biofilm formation.</p></sec><sec id="S2"><title>Materials and methods</title><p id="P2">Nine bacterial species belonging to the normal oral microbiota, including: Aggregatibacter actinomycetemcomitans, Actinomyces israelii, Campylobacter rectus, Eikenella corrodens, Fusobacterium nucleatum, Parvimonas micra, Porphyromonas gingivalis, Prevotella intermedia, and <italic>Streptococcus sanguinis</italic> were tested on Ti surfaces: pretreatment (PT [<italic>R</italic><sub>a</sub><0.2 μm]), acid-etched (A [<italic>R</italic><sub>a</sub><0.8 μm]), A modified to be hydrophilic (modA), sand-blasted/acid-etched (SLA [<italic>R</italic><sub>a</sub> = 4 μm]), and hydrophilic SLA (modSLA). Disks were incubated for 24 h in anaerobic conditions using a normal culture medium (CM) or human saliva (HS). The total counts of bacteria and the proportion of each bacterial species were analysed by checkerboard DNA–DNA hybridization. Results: Higher counts of bacteria were observed on all surfaces incubated with CM compared with the samples incubated with HS. PT, SLA, and modSLA exhibited higher numbers of attached bacteria in CM, whereas SLA and modSLA had a significant increase in bacterial adhesion in HS. The proportion of the species in the initial biofilms was also influenced by the surface properties and the media used: SLA and modSLA increased the proportion of species like A. <italic>actinomycetemcomitans</italic> and <italic>S. sanguinis</italic> in both media, while the adhesion of <italic>A. israelii</italic> and <italic>P. gingivalis</italic> on the same surfaces was affected in the presence of saliva.</p></sec><sec id="S3"><title>Conclusions</title><p id="P3">The initial biofilm formation and composition were affected by the microtopography and hydrophilicity of the surface and by the media used.</p></sec></div></front></TEI></PMC></double></record>Pour manipuler ce document sous Unix (Dilib)
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