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Collection and Extraction of Saliva DNA for Next Generation Sequencing

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Collection and Extraction of Saliva DNA for Next Generation Sequencing

Auteurs : Michael R. Goode ; Soo Yeon Cheong ; Ning Li ; William C. Ray ; Christopher W. Bartlett

Source :

Journal of Visualized Experiments : JoVE [ 1940-087X ] ; 2014.

RBID : PMC:4442313

Abstract

The preferred source of DNA in human genetics research is blood, or cell lines derived from blood, as these sources yield large quantities of high quality DNA. However, DNA extraction from saliva can yield high quality DNA with little to no degradation/fragmentation that is suitable for a variety of DNA assays without the expense of a phlebotomist and can even be acquired through the mail. However, at present, no saliva DNA collection/extraction protocols for next generation sequencing have been presented in the literature. This protocol optimizes parameters of saliva collection/storage and DNA extraction to be of sufficient quality and quantity for DNA assays with the highest standards, including microarray genotyping and next generation sequencing.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4442313

DOI: 10.3791/51697
PubMed: 25225892
PubMed Central: 4442313

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PMC:4442313

Le document en format XML

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<author><name sortKey="Li, Ning" sort="Li, Ning" uniqKey="Li N" first="Ning" last="Li">Ning Li</name>
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<affiliation><nlm:aff id="ID2">The Interdisciplinary Graduate Program in Biophysics, The Ohio State University</nlm:aff>
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<front><div type="abstract" xml:lang="en"><p>The preferred source of DNA in human genetics research is blood, or cell lines derived from blood, as these sources yield large quantities of high quality DNA. However, DNA extraction from saliva can yield high quality DNA with little to no degradation/fragmentation that is suitable for a variety of DNA assays without the expense of a phlebotomist and can even be acquired through the mail. However, at present, no saliva DNA collection/extraction protocols for next generation sequencing have been presented in the literature. This protocol optimizes parameters of saliva collection/storage and DNA extraction to be of sufficient quality and quantity for DNA assays with the highest standards, including microarray genotyping and next generation sequencing.</p>
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Collection and Extraction of Saliva DNA for Next Generation Sequencing

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki