Adhesion of fibroblasts on micro- and nanostructured surfaces prepared by chemical vapor deposition and pulsed laser treatment
Identifieur interne : 005457 ( Main/Exploration ); précédent : 005456; suivant : 005458Adhesion of fibroblasts on micro- and nanostructured surfaces prepared by chemical vapor deposition and pulsed laser treatment
Auteurs : M. Veith [Allemagne] ; O C Aktas [Allemagne] ; W. Metzger [Allemagne] ; D. Sossong [Allemagne] ; H. Ullah Wazir [Allemagne] ; I. Grobelsek [Allemagne] ; N. Ptz [Allemagne] ; G. Wennemuth [Allemagne] ; T. Pohlemann [Allemagne] ; M. Oberringer [Allemagne]Source :
- Biofabrication [ 1758-5082 ] ; 2010.
Descripteurs français
- Wicri :
- topic : Titane.
English descriptors
- KwdEn :
- Adhesion, Biofabrication, Biomed, Broblasts, Cell compatibility, Cell density, Cell morphology, Ceramic materials, Chemical composition, Chemical vapor deposition, Compatibility, Contact angle, Focal adhesion sites, Glass control, Gure, Gures, Highest cell density, Implant, Implant surface, Laser, Laser pulse, Laser pulses, Laser treatment, Lopodia, Mater, Microstructures, Mitotic, Mitotic cells, Morphology, Nano, Nanostructured, Nanowires, Nhdf, Osteoblast, Other substrates, Permanent implants, Pulse, Scale bars, Single laser pulse, Spherical particles, Such nanowires, Surface topography, Third laser pulse, Titanium, Unusual morphology, Veith.
- Teeft :
- Adhesion, Biofabrication, Biomed, Broblasts, Cell compatibility, Cell density, Cell morphology, Ceramic materials, Chemical composition, Chemical vapor deposition, Compatibility, Contact angle, Focal adhesion sites, Glass control, Gure, Gures, Highest cell density, Implant, Implant surface, Laser, Laser pulse, Laser pulses, Laser treatment, Lopodia, Mater, Microstructures, Mitotic, Mitotic cells, Morphology, Nano, Nanostructured, Nanowires, Nhdf, Osteoblast, Other substrates, Permanent implants, Pulse, Scale bars, Single laser pulse, Spherical particles, Such nanowires, Surface topography, Third laser pulse, Titanium, Unusual morphology, Veith.
Abstract
The development of micro- and nanostructured surfaces which improve the cellsubstrate interaction is of great interest in today's implant applications. In this regard, AlAl2O3 bi-phasic nanowires were synthesized by chemical vapor deposition of the molecular precursor (tBuOAlH2)2. Heat treatment of such bi-phasic nanowires with short laser pulses leads to micro- and nanostructured Al2O3 surfaces. Such surfaces were characterized by scanning electron microscopy (SEM), electron dispersive spectroscopy and x-ray photoelectron spectroscopy. Following the detailed material characterization, the prepared surfaces were tested for their cell compatibility using normal human dermal fibroblasts. While the cells cultivated on AlAl2O3 bi-phasic nanowires showed an unusual morphology, cells cultivated on nanowires treated with one and two laser pulses exhibited morphologies similar to those observed on the control substrate. The highest cell density was observed on surfaces treated with one laser pulse. The interaction of the cells with the nano- and microstructures was investigated by SEM analysis in detail. Laser treatment of AlAl2O3 bi-phasic nanowires is a fast and easy method to fabricate nano- and microstructured Al2O3-surfaces for studying cellsurface interactions. It is our goal to develop a biocompatible Al2O3-surface which could be used as a coating material for medical implants exhibiting a cell selective response because of its specific physical landscape and especially because it promotes the adhesion of osteoblasts while minimizing the adhesion of fibroblasts.
Url:
DOI: 10.1088/1758-5082/2/3/035001
Affiliations:
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Oberringer</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Trauma-, Hand- and Reconstructive Surgery, Saarland University, Kirrberger Strae, Building 57, 66421 Homburg</wicri:regionArea><wicri:noRegion>66421 Homburg</wicri:noRegion><wicri:noRegion>66421 Homburg</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Biofabrication</title><title level="j" type="abbrev">Biofabrication</title><idno type="ISSN">1758-5082</idno><imprint><publisher>IOP Publishing</publisher><date type="published" when="2010">2010</date><biblScope unit="volume">2</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="1">1</biblScope><biblScope unit="page" to="10">10</biblScope><biblScope unit="production">Printed in the UK</biblScope></imprint><idno type="ISSN">1758-5082</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1758-5082</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adhesion</term><term>Biofabrication</term><term>Biomed</term><term>Broblasts</term><term>Cell compatibility</term><term>Cell density</term><term>Cell morphology</term><term>Ceramic materials</term><term>Chemical composition</term><term>Chemical vapor deposition</term><term>Compatibility</term><term>Contact angle</term><term>Focal adhesion sites</term><term>Glass control</term><term>Gure</term><term>Gures</term><term>Highest cell density</term><term>Implant</term><term>Implant surface</term><term>Laser</term><term>Laser pulse</term><term>Laser pulses</term><term>Laser treatment</term><term>Lopodia</term><term>Mater</term><term>Microstructures</term><term>Mitotic</term><term>Mitotic cells</term><term>Morphology</term><term>Nano</term><term>Nanostructured</term><term>Nanowires</term><term>Nhdf</term><term>Osteoblast</term><term>Other substrates</term><term>Permanent implants</term><term>Pulse</term><term>Scale bars</term><term>Single laser pulse</term><term>Spherical particles</term><term>Such nanowires</term><term>Surface topography</term><term>Third laser pulse</term><term>Titanium</term><term>Unusual morphology</term><term>Veith</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Adhesion</term><term>Biofabrication</term><term>Biomed</term><term>Broblasts</term><term>Cell compatibility</term><term>Cell density</term><term>Cell morphology</term><term>Ceramic materials</term><term>Chemical composition</term><term>Chemical vapor deposition</term><term>Compatibility</term><term>Contact angle</term><term>Focal adhesion sites</term><term>Glass control</term><term>Gure</term><term>Gures</term><term>Highest cell density</term><term>Implant</term><term>Implant surface</term><term>Laser</term><term>Laser pulse</term><term>Laser pulses</term><term>Laser treatment</term><term>Lopodia</term><term>Mater</term><term>Microstructures</term><term>Mitotic</term><term>Mitotic cells</term><term>Morphology</term><term>Nano</term><term>Nanostructured</term><term>Nanowires</term><term>Nhdf</term><term>Osteoblast</term><term>Other substrates</term><term>Permanent implants</term><term>Pulse</term><term>Scale bars</term><term>Single laser pulse</term><term>Spherical particles</term><term>Such nanowires</term><term>Surface topography</term><term>Third laser pulse</term><term>Titanium</term><term>Unusual morphology</term><term>Veith</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Titane</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">The development of micro- and nanostructured surfaces which improve the cellsubstrate interaction is of great interest in today's implant applications. In this regard, AlAl2O3 bi-phasic nanowires were synthesized by chemical vapor deposition of the molecular precursor (tBuOAlH2)2. Heat treatment of such bi-phasic nanowires with short laser pulses leads to micro- and nanostructured Al2O3 surfaces. Such surfaces were characterized by scanning electron microscopy (SEM), electron dispersive spectroscopy and x-ray photoelectron spectroscopy. Following the detailed material characterization, the prepared surfaces were tested for their cell compatibility using normal human dermal fibroblasts. While the cells cultivated on AlAl2O3 bi-phasic nanowires showed an unusual morphology, cells cultivated on nanowires treated with one and two laser pulses exhibited morphologies similar to those observed on the control substrate. The highest cell density was observed on surfaces treated with one laser pulse. The interaction of the cells with the nano- and microstructures was investigated by SEM analysis in detail. Laser treatment of AlAl2O3 bi-phasic nanowires is a fast and easy method to fabricate nano- and microstructured Al2O3-surfaces for studying cellsurface interactions. It is our goal to develop a biocompatible Al2O3-surface which could be used as a coating material for medical implants exhibiting a cell selective response because of its specific physical landscape and especially because it promotes the adhesion of osteoblasts while minimizing the adhesion of fibroblasts.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Sarre (Land)</li></region><settlement><li>Sarrebruck</li></settlement></list><tree><country name="Allemagne"><region name="Sarre (Land)"><name sortKey="Veith, M" sort="Veith, M" uniqKey="Veith M" first="M" last="Veith">M. Veith</name></region><name sortKey="Aktas, O C" sort="Aktas, O C" uniqKey="Aktas O" first="O C" last="Aktas">O C Aktas</name><name sortKey="Grobelsek, I" sort="Grobelsek, I" uniqKey="Grobelsek I" first="I" last="Grobelsek">I. Grobelsek</name><name sortKey="Metzger, W" sort="Metzger, W" uniqKey="Metzger W" first="W" last="Metzger">W. Metzger</name><name sortKey="Oberringer, M" sort="Oberringer, M" uniqKey="Oberringer M" first="M" last="Oberringer">M. Oberringer</name><name sortKey="Pohlemann, T" sort="Pohlemann, T" uniqKey="Pohlemann T" first="T" last="Pohlemann">T. Pohlemann</name><name sortKey="Ptz, N" sort="Ptz, N" uniqKey="Ptz N" first="N" last="Ptz">N. Ptz</name><name sortKey="Sossong, D" sort="Sossong, D" uniqKey="Sossong D" first="D" last="Sossong">D. Sossong</name><name sortKey="Ullah Wazir, H" sort="Ullah Wazir, H" uniqKey="Ullah Wazir H" first="H" last="Ullah Wazir">H. Ullah Wazir</name><name sortKey="Veith, M" sort="Veith, M" uniqKey="Veith M" first="M" last="Veith">M. Veith</name><name sortKey="Wennemuth, G" sort="Wennemuth, G" uniqKey="Wennemuth G" first="G" last="Wennemuth">G. Wennemuth</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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