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Analysis of osteoblastic gene expression in the early human mesenchymal cell response to a chemically modified implant surface: an in vitro study

Identifieur interne : 004980 ( Main/Exploration ); précédent : 004979; suivant : 004981

Analysis of osteoblastic gene expression in the early human mesenchymal cell response to a chemically modified implant surface: an in vitro study

Auteurs : Anastasios A. Mamalis [États-Unis] ; Spyridon S. Silvestros [Grèce]

Source :

RBID : ISTEX:146F241D4C1636F31051C482FFE7DCBE5B3C4CAE

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English descriptors

Abstract

Objectives: The effect of a chemical modification of the SLA surface (SLActive surface) on human bone marrow‐derived mesenchymal cells (hMSCs) on; (1) adhesion, (2) proliferation and (3) early transcriptional control of osteogenic differentiation was investigated. We are based on the hypothesis that expression patterns of genes responsible for osteogenesis might be dependent on the characteristics of the implant surface.

Url:
DOI: 10.1111/j.1600-0501.2010.02049.x


Affiliations:


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Le document en format XML

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<term>Assay</term>
<term>Biomedical</term>
<term>Biomedical materials research</term>
<term>Bone formation</term>
<term>Bone response</term>
<term>Boyan</term>
<term>Campomelic dysplasia</term>
<term>Cdna</term>
<term>Cdna gearrayt</term>
<term>Cell adhesion</term>
<term>Cell attachment</term>
<term>Cell counts</term>
<term>Cell cycle regulation</term>
<term>Cell density</term>
<term>Cell differentiation</term>
<term>Cell growth</term>
<term>Cellular biochemistry</term>
<term>Clin</term>
<term>Cochran</term>
<term>Collagen</term>
<term>Collagen type</term>
<term>Control surface</term>
<term>Culture medium</term>
<term>Dental implants</term>
<term>Drosophila</term>
<term>Early gene expression</term>
<term>Early osteogenesis</term>
<term>Early osteogenesis gene response</term>
<term>Early proliferation</term>
<term>Early stages</term>
<term>Experimental study</term>
<term>Extracellular matrix</term>
<term>Fibroblast growth factor receptor</term>
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<term>Gene expression</term>
<term>Growth factor</term>
<term>Health science center</term>
<term>Histomorphometric analysis</term>
<term>Hmsc</term>
<term>Hmsc cells</term>
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<term>Human mesenchymal</term>
<term>Human osteogenesis</term>
<term>Human osteogenesis gene array</term>
<term>Human rhinovirus receptor growth factor</term>
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<term>Implant</term>
<term>Implant surface</term>
<term>Implant surfaces</term>
<term>Implants research</term>
<term>Initial attachment</term>
<term>Initial cell attachment</term>
<term>Intercellular adhesion molecule</term>
<term>International journal</term>
<term>Isotonic nacl solution</term>
<term>John wiley sons</term>
<term>Kinase</term>
<term>Mamalis</term>
<term>Mamalis silvestros chemical</term>
<term>Maxillofacial surgery</term>
<term>Mesenchymal</term>
<term>Mesenchymal cell response</term>
<term>Microarray</term>
<term>Microarray analysis</term>
<term>Mineral research</term>
<term>Nuclear factor</term>
<term>Oral impl</term>
<term>Oral maxillofacial implants</term>
<term>Osseointegration</term>
<term>Osteoblast</term>
<term>Osteoblastic</term>
<term>Osteoblastic cells</term>
<term>Osteoblastic gene expression</term>
<term>Osteogenesis</term>
<term>Osteogenesis gene array</term>
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<term>Osteogenic differentiation</term>
<term>Pfeiffer syndrome</term>
<term>Polypeptide</term>
<term>Position unigene genebank symbol description gene name</term>
<term>Present study</term>
<term>Previous studies</term>
<term>Proliferation</term>
<term>Protein kinase</term>
<term>Protein receptor</term>
<term>Protein synthesis</term>
<term>Receptor</term>
<term>Reduction assay</term>
<term>Relative percent change</term>
<term>Rough surface</term>
<term>Roughness</term>
<term>Signal transduction</term>
<term>Silvestros</term>
<term>Slactive</term>
<term>Slactive implant surface</term>
<term>Slactive implant surfaces</term>
<term>Slactive surface</term>
<term>Slactive surfaces</term>
<term>Smad</term>
<term>Smooth surface</term>
<term>Smooth surfaces</term>
<term>Surface microtopography</term>
<term>Surface roughness</term>
<term>Surface roughness modulates</term>
<term>Syndrome type</term>
<term>Thrombospondin receptor</term>
<term>Time course</term>
<term>Time interval</term>
<term>Titanium</term>
<term>Titanium implants</term>
<term>Titanium surface</term>
<term>Titanium surface roughness</term>
<term>Twist homolog</term>
<term>Tyrosine kinase</term>
<term>Upregulated</term>
<term>Upregulated genes control cell differentiation</term>
<term>Vascular cell adhesion molecule</term>
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<term>Absolute cell numbers</term>
<term>Adhesion</term>
<term>Alpha collagen</term>
<term>Assay</term>
<term>Biomedical</term>
<term>Biomedical materials research</term>
<term>Bone formation</term>
<term>Bone response</term>
<term>Boyan</term>
<term>Campomelic dysplasia</term>
<term>Cdna</term>
<term>Cdna gearrayt</term>
<term>Cell adhesion</term>
<term>Cell attachment</term>
<term>Cell counts</term>
<term>Cell cycle regulation</term>
<term>Cell density</term>
<term>Cell differentiation</term>
<term>Cell growth</term>
<term>Cellular biochemistry</term>
<term>Clin</term>
<term>Cochran</term>
<term>Collagen</term>
<term>Collagen type</term>
<term>Control surface</term>
<term>Culture medium</term>
<term>Dental implants</term>
<term>Drosophila</term>
<term>Early gene expression</term>
<term>Early osteogenesis</term>
<term>Early osteogenesis gene response</term>
<term>Early proliferation</term>
<term>Early stages</term>
<term>Experimental study</term>
<term>Extracellular matrix</term>
<term>Fibroblast growth factor receptor</term>
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<term>Gene expression</term>
<term>Growth factor</term>
<term>Health science center</term>
<term>Histomorphometric analysis</term>
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<term>Hmsc cells</term>
<term>Hmscs</term>
<term>Homolog</term>
<term>Human bone mesenchymal cells</term>
<term>Human mesenchymal</term>
<term>Human osteogenesis</term>
<term>Human osteogenesis gene array</term>
<term>Human rhinovirus receptor growth factor</term>
<term>Impl</term>
<term>Implant</term>
<term>Implant surface</term>
<term>Implant surfaces</term>
<term>Implants research</term>
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<term>Initial cell attachment</term>
<term>Intercellular adhesion molecule</term>
<term>International journal</term>
<term>Isotonic nacl solution</term>
<term>John wiley sons</term>
<term>Kinase</term>
<term>Mamalis</term>
<term>Mamalis silvestros chemical</term>
<term>Maxillofacial surgery</term>
<term>Mesenchymal</term>
<term>Mesenchymal cell response</term>
<term>Microarray</term>
<term>Microarray analysis</term>
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<term>Nuclear factor</term>
<term>Oral impl</term>
<term>Oral maxillofacial implants</term>
<term>Osseointegration</term>
<term>Osteoblast</term>
<term>Osteoblastic</term>
<term>Osteoblastic cells</term>
<term>Osteoblastic gene expression</term>
<term>Osteogenesis</term>
<term>Osteogenesis gene array</term>
<term>Osteogenic</term>
<term>Osteogenic differentiation</term>
<term>Pfeiffer syndrome</term>
<term>Polypeptide</term>
<term>Position unigene genebank symbol description gene name</term>
<term>Present study</term>
<term>Previous studies</term>
<term>Proliferation</term>
<term>Protein kinase</term>
<term>Protein receptor</term>
<term>Protein synthesis</term>
<term>Receptor</term>
<term>Reduction assay</term>
<term>Relative percent change</term>
<term>Rough surface</term>
<term>Roughness</term>
<term>Signal transduction</term>
<term>Silvestros</term>
<term>Slactive</term>
<term>Slactive implant surface</term>
<term>Slactive implant surfaces</term>
<term>Slactive surface</term>
<term>Slactive surfaces</term>
<term>Smad</term>
<term>Smooth surface</term>
<term>Smooth surfaces</term>
<term>Surface microtopography</term>
<term>Surface roughness</term>
<term>Surface roughness modulates</term>
<term>Syndrome type</term>
<term>Thrombospondin receptor</term>
<term>Time course</term>
<term>Time interval</term>
<term>Titanium</term>
<term>Titanium implants</term>
<term>Titanium surface</term>
<term>Titanium surface roughness</term>
<term>Twist homolog</term>
<term>Tyrosine kinase</term>
<term>Upregulated</term>
<term>Upregulated genes control cell differentiation</term>
<term>Vascular cell adhesion molecule</term>
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<front>
<div type="abstract">Objectives: The effect of a chemical modification of the SLA surface (SLActive surface) on human bone marrow‐derived mesenchymal cells (hMSCs) on; (1) adhesion, (2) proliferation and (3) early transcriptional control of osteogenic differentiation was investigated. We are based on the hypothesis that expression patterns of genes responsible for osteogenesis might be dependent on the characteristics of the implant surface.</div>
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