Expression of Mn‐SOD, iNOS and eNOS mRNAs in Osteoblasts from the Maxilla of Osteopetrotic Mice
Identifieur interne : 002C16 ( Main/Exploration ); précédent : 002C15; suivant : 002C17Expression of Mn‐SOD, iNOS and eNOS mRNAs in Osteoblasts from the Maxilla of Osteopetrotic Mice
Auteurs : M. Kuwana [Japon] ; H. Watanabe [Japon] ; S. Abe [Japon] ; T. Yanagisawa [Japon] ; J. Sasaki [Japon]Source :
- Anatomia, Histologia, Embryologia [ 0340-2096 ] ; 2013-04.
English descriptors
- KwdEn :
- Active oxygens, Anat, Blackwell verlag gmbh anat, Bone marrow, Cell membrane, Control group, Control mice, Enos, Enos mrna expression, Enos mrna signal, Free radicals, Hybridisation, Inos, Inos mrna expression, Inos mrna signal, Maxilla, Maxilla bones, Maxilla osteoblasts, Mrna, Mrna expression, Mrna signal, Nitric, Nitric oxide, Normal osteoblasts, Osteoblast, Osteopetrotic, Osteopetrotic mice, Osteopetrotic model mice, Periosteum, Protein kinase, Silver particles, Similar results, Watanabe.
- Teeft :
- Active oxygens, Anat, Blackwell verlag gmbh anat, Bone marrow, Cell membrane, Control group, Control mice, Enos, Enos mrna expression, Enos mrna signal, Free radicals, Hybridisation, Inos, Inos mrna expression, Inos mrna signal, Maxilla, Maxilla bones, Maxilla osteoblasts, Mrna, Mrna expression, Mrna signal, Nitric, Nitric oxide, Normal osteoblasts, Osteoblast, Osteopetrotic, Osteopetrotic mice, Osteopetrotic model mice, Periosteum, Protein kinase, Silver particles, Similar results, Watanabe.
Abstract
Active oxygens and free radicals are involved in the metabolism and clinical conditions of tissues; however, little is known about the localisation and expression levels of associated enzymes. The expressions of active oxygens, free radicals and associated enzymes are reported to be site‐specific; therefore, the expression states of free radical enzymes differ between sites, even within the same cell. In particular, there has been no report concerning the catabolic enzymes of active oxygens in osteoblasts of the maxilla, other than normal osteoblasts that were weakly positive by immunohistochemical staining. We conducted this study to elucidate the relationship between osteodystrophy and radical‐associated enzymes by investigating mRNAs of enzymes associated with active oxygens and free radicals using osteoblasts from the maxilla of normal and osteopetrotic model (op/op) mice. In op/op mouse maxilla osteoblasts, mRNAs of Mn‐SOD, i‐NOS and e‐NOS were strongly positive. Mn‐SOD and iNOS enzymes were considered to be highly expressed in osteoblasts of the narrowed medullary cavity of this bone.
Url:
DOI: 10.1111/j.1439-0264.2012.01173.x
Affiliations:
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Abe</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Anatomy, Tokyo Dental College, Masago 1‐2‐2, 261‐8502, MihamakuChiba‐cityChiba</wicri:regionArea><wicri:noRegion>MihamakuChiba‐cityChiba</wicri:noRegion></affiliation></author><author><name sortKey="Yanagisawa, T" sort="Yanagisawa, T" uniqKey="Yanagisawa T" first="T." last="Yanagisawa">T. Yanagisawa</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Ultrastructural Science, Tokyo Dental College, Masago 1‐2‐2, 261‐8502, MihamakuChiba‐CityChiba</wicri:regionArea><wicri:noRegion>MihamakuChiba‐CityChiba</wicri:noRegion></affiliation></author><author><name sortKey="Sasaki, J" sort="Sasaki, J" uniqKey="Sasaki J" first="J." last="Sasaki">J. Sasaki</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2‐5‐1 Shikatacho, 700‐8558, Okayama</wicri:regionArea><wicri:noRegion>Okayama</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Anatomia, Histologia, Embryologia</title><title level="j" type="alt">ANATOMIA, HISTOLOGIA, EMBRYOLOGIA</title><idno type="ISSN">0340-2096</idno><idno type="eISSN">1439-0264</idno><imprint><biblScope unit="vol">42</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="124">124</biblScope><biblScope unit="page" to="129">129</biblScope><biblScope unit="page-count">6</biblScope><date type="published" when="2013-04">2013-04</date></imprint><idno type="ISSN">0340-2096</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0340-2096</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Active oxygens</term><term>Anat</term><term>Blackwell verlag gmbh anat</term><term>Bone marrow</term><term>Cell membrane</term><term>Control group</term><term>Control mice</term><term>Enos</term><term>Enos mrna expression</term><term>Enos mrna signal</term><term>Free radicals</term><term>Hybridisation</term><term>Inos</term><term>Inos mrna expression</term><term>Inos mrna signal</term><term>Maxilla</term><term>Maxilla bones</term><term>Maxilla osteoblasts</term><term>Mrna</term><term>Mrna expression</term><term>Mrna signal</term><term>Nitric</term><term>Nitric oxide</term><term>Normal osteoblasts</term><term>Osteoblast</term><term>Osteopetrotic</term><term>Osteopetrotic mice</term><term>Osteopetrotic model mice</term><term>Periosteum</term><term>Protein kinase</term><term>Silver particles</term><term>Similar results</term><term>Watanabe</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Active oxygens</term><term>Anat</term><term>Blackwell verlag gmbh anat</term><term>Bone marrow</term><term>Cell membrane</term><term>Control group</term><term>Control mice</term><term>Enos</term><term>Enos mrna expression</term><term>Enos mrna signal</term><term>Free radicals</term><term>Hybridisation</term><term>Inos</term><term>Inos mrna expression</term><term>Inos mrna signal</term><term>Maxilla</term><term>Maxilla bones</term><term>Maxilla osteoblasts</term><term>Mrna</term><term>Mrna expression</term><term>Mrna signal</term><term>Nitric</term><term>Nitric oxide</term><term>Normal osteoblasts</term><term>Osteoblast</term><term>Osteopetrotic</term><term>Osteopetrotic mice</term><term>Osteopetrotic model mice</term><term>Periosteum</term><term>Protein kinase</term><term>Silver particles</term><term>Similar results</term><term>Watanabe</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">Active oxygens and free radicals are involved in the metabolism and clinical conditions of tissues; however, little is known about the localisation and expression levels of associated enzymes. The expressions of active oxygens, free radicals and associated enzymes are reported to be site‐specific; therefore, the expression states of free radical enzymes differ between sites, even within the same cell. In particular, there has been no report concerning the catabolic enzymes of active oxygens in osteoblasts of the maxilla, other than normal osteoblasts that were weakly positive by immunohistochemical staining. We conducted this study to elucidate the relationship between osteodystrophy and radical‐associated enzymes by investigating mRNAs of enzymes associated with active oxygens and free radicals using osteoblasts from the maxilla of normal and osteopetrotic model (op/op) mice. In op/op mouse maxilla osteoblasts, mRNAs of Mn‐SOD, i‐NOS and e‐NOS were strongly positive. Mn‐SOD and iNOS enzymes were considered to be highly expressed in osteoblasts of the narrowed medullary cavity of this bone.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Kuwana, M" sort="Kuwana, M" uniqKey="Kuwana M" first="M." last="Kuwana">M. Kuwana</name></noRegion><name sortKey="Abe, S" sort="Abe, S" uniqKey="Abe S" first="S." last="Abe">S. Abe</name><name sortKey="Sasaki, J" sort="Sasaki, J" uniqKey="Sasaki J" first="J." last="Sasaki">J. Sasaki</name><name sortKey="Watanabe, H" sort="Watanabe, H" uniqKey="Watanabe H" first="H." last="Watanabe">H. Watanabe</name><name sortKey="Watanabe, H" sort="Watanabe, H" uniqKey="Watanabe H" first="H." last="Watanabe">H. Watanabe</name><name sortKey="Yanagisawa, T" sort="Yanagisawa, T" uniqKey="Yanagisawa T" first="T." last="Yanagisawa">T. Yanagisawa</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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