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Ultrastructural and immunohistochemical study of early repair of alveolar sockets after the extraction of molars from alendronate‐treated rats

Identifieur interne : 002808 ( Main/Exploration ); précédent : 002807; suivant : 002809

Ultrastructural and immunohistochemical study of early repair of alveolar sockets after the extraction of molars from alendronate‐treated rats

Auteurs : Fernanda P. Yamamoto-Silva [Brésil] ; Vivian Bradaschia-Correa [Brésil] ; Luiz A. P. A. Lima [Brésil] ; Victor E. Arana-Chavez [Brésil]

Source :

RBID : ISTEX:23686ABC3314E334A2B859331F72A443DD43E875

English descriptors

Abstract

The aim of the present research was to analyze ultrastructural and immunohistochemical aspects of the alveolar repair after the extraction of molars of alendronate (ALN)‐treated rats. Wistar rats received 2.5mg/kg body wt/day of ALN during 14 days previously and 7, 14 and 21 days after the extraction of the second mandibular molar. Specimens were fixed in 2% glutaraldehyde + 2.5% formaldehyde under microwave irradiation, decalcified in 4.13% EDTA and paraffin embedded for TRAP histochemistry and immunohistochemistry for OPN, BSP and endoglin, or embedded in Spurr epoxy resin for TEM analysis. Additional specimens had their soft tissues removed and were processed for scanning electron microscopy. The ALN group presented latent TRAP‐positive osteoclasts and nonresorbed alveolar crests with bacterial infection. Mild bone necrosis signs were observed at all time points studied. Ultrastructurally, empty osteocyte lacunae were observed and bone trabeculae surface presented hyalinized aspect. A significant delay in alveolar repair occurred, as well as decreased angiogenesis. ALN treatment provoked mild signs of bone necrosis, despite the high dose employed. The present findings add new information about the ultrastructural aspect of the early repair of rats under ALN treatment and highlight for giving attention when oral surgeries are performed in patients using this drug. Microsc. Res. Tech. 76:633–640, 2013. © 2013 Wiley Periodicals, Inc.

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DOI: 10.1002/jemt.22210


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<term>Active osteoblasts</term>
<term>Active osteoclasts</term>
<term>Alendronate</term>
<term>Alveolar</term>
<term>Alveolar bone</term>
<term>Alveolar bone crest</term>
<term>Alveolar bone repair</term>
<term>Alveolar crest</term>
<term>Alveolar crests</term>
<term>Alveolar healing</term>
<term>Alveolar healing process</term>
<term>Alveolar repair</term>
<term>Alveolar socket</term>
<term>Alveolar sockets</term>
<term>Alveolar wall</term>
<term>Alveolar walls</term>
<term>Alveolus</term>
<term>American association</term>
<term>Apical</term>
<term>Apical region</term>
<term>Aseptic necrosis</term>
<term>Bacterial contamination</term>
<term>Bacterial infection</term>
<term>Bacterial infection foci</term>
<term>Biochem cell biol</term>
<term>Bisphosphonate</term>
<term>Bisphosphonate therapy</term>
<term>Bisphosphonates</term>
<term>Blood vessels</term>
<term>Bone formation</term>
<term>Bone healing</term>
<term>Bone matrix</term>
<term>Bone necrosis</term>
<term>Bone resorption</term>
<term>Bone surfaces</term>
<term>Bone trabeculae</term>
<term>Cement lines</term>
<term>Cervical</term>
<term>Cervical area</term>
<term>Cervical portion</term>
<term>Clinical reports</term>
<term>Connective tissue</term>
<term>Early repair</term>
<term>Empty osteocyte lacunae</term>
<term>Extraction</term>
<term>Healing process</term>
<term>High dose</term>
<term>Histomorphometric analysis</term>
<term>Hyalinized aspect</term>
<term>Hyalinized material</term>
<term>Immature bone</term>
<term>Immature bone matrix</term>
<term>Immature bone trabeculae</term>
<term>Lacuna</term>
<term>Larry fisher</term>
<term>Light micrographs</term>
<term>Light microscope</term>
<term>Lled</term>
<term>Lower bone formation</term>
<term>Matrix</term>
<term>Microscopy</term>
<term>Microscopy research</term>
<term>Microwave irradiation</term>
<term>Mild signs</term>
<term>Mineralized matrix</term>
<term>Molar</term>
<term>Neoformed bone trabeculae</term>
<term>Noncollagenous matrix proteins</term>
<term>Numerous spaces</term>
<term>Online issue</term>
<term>Oral biology</term>
<term>Oral pathol</term>
<term>Oral surg</term>
<term>Oral surgeries</term>
<term>Orthodontic movement</term>
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<term>Osteoblast function</term>
<term>Osteoclast</term>
<term>Osteocyte</term>
<term>Osteonecrosis</term>
<term>Paulo</term>
<term>Periodontal ligament</term>
<term>Present study</term>
<term>Pressure areas</term>
<term>Pressure zones</term>
<term>Rat</term>
<term>Resorption</term>
<term>Resorption lacunae</term>
<term>Room temperature</term>
<term>Scanning electron microscopy</term>
<term>Second mandibular molar</term>
<term>Second molar</term>
<term>Socket</term>
<term>Technique alendronate effects</term>
<term>Thin bone trabeculae</term>
<term>Thin layer</term>
<term>Time point</term>
<term>Time points</term>
<term>Tooth extraction</term>
<term>Trabecula</term>
<term>Transmission electron microscopy</term>
<term>Trap histochemistry</term>
<term>Ultrastructural</term>
<term>Wiley periodicals</term>
<term>Wistar rats</term>
<term>Xative solution</term>
<term>Zoledronic</term>
<term>Zoledronic acid</term>
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<term>Active osteoblasts</term>
<term>Active osteoclasts</term>
<term>Alendronate</term>
<term>Alveolar</term>
<term>Alveolar bone</term>
<term>Alveolar bone crest</term>
<term>Alveolar bone repair</term>
<term>Alveolar crest</term>
<term>Alveolar crests</term>
<term>Alveolar healing</term>
<term>Alveolar healing process</term>
<term>Alveolar repair</term>
<term>Alveolar socket</term>
<term>Alveolar sockets</term>
<term>Alveolar wall</term>
<term>Alveolar walls</term>
<term>Alveolus</term>
<term>American association</term>
<term>Apical</term>
<term>Apical region</term>
<term>Aseptic necrosis</term>
<term>Bacterial contamination</term>
<term>Bacterial infection</term>
<term>Bacterial infection foci</term>
<term>Biochem cell biol</term>
<term>Bisphosphonate</term>
<term>Bisphosphonate therapy</term>
<term>Bisphosphonates</term>
<term>Blood vessels</term>
<term>Bone formation</term>
<term>Bone healing</term>
<term>Bone matrix</term>
<term>Bone necrosis</term>
<term>Bone resorption</term>
<term>Bone surfaces</term>
<term>Bone trabeculae</term>
<term>Cement lines</term>
<term>Cervical</term>
<term>Cervical area</term>
<term>Cervical portion</term>
<term>Clinical reports</term>
<term>Connective tissue</term>
<term>Early repair</term>
<term>Empty osteocyte lacunae</term>
<term>Extraction</term>
<term>Healing process</term>
<term>High dose</term>
<term>Histomorphometric analysis</term>
<term>Hyalinized aspect</term>
<term>Hyalinized material</term>
<term>Immature bone</term>
<term>Immature bone matrix</term>
<term>Immature bone trabeculae</term>
<term>Lacuna</term>
<term>Larry fisher</term>
<term>Light micrographs</term>
<term>Light microscope</term>
<term>Lled</term>
<term>Lower bone formation</term>
<term>Matrix</term>
<term>Microscopy</term>
<term>Microscopy research</term>
<term>Microwave irradiation</term>
<term>Mild signs</term>
<term>Mineralized matrix</term>
<term>Molar</term>
<term>Neoformed bone trabeculae</term>
<term>Noncollagenous matrix proteins</term>
<term>Numerous spaces</term>
<term>Online issue</term>
<term>Oral biology</term>
<term>Oral pathol</term>
<term>Oral surg</term>
<term>Oral surgeries</term>
<term>Orthodontic movement</term>
<term>Osteoblast</term>
<term>Osteoblast function</term>
<term>Osteoclast</term>
<term>Osteocyte</term>
<term>Osteonecrosis</term>
<term>Paulo</term>
<term>Periodontal ligament</term>
<term>Present study</term>
<term>Pressure areas</term>
<term>Pressure zones</term>
<term>Rat</term>
<term>Resorption</term>
<term>Resorption lacunae</term>
<term>Room temperature</term>
<term>Scanning electron microscopy</term>
<term>Second mandibular molar</term>
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<term>Socket</term>
<term>Technique alendronate effects</term>
<term>Thin bone trabeculae</term>
<term>Thin layer</term>
<term>Time point</term>
<term>Time points</term>
<term>Tooth extraction</term>
<term>Trabecula</term>
<term>Transmission electron microscopy</term>
<term>Trap histochemistry</term>
<term>Ultrastructural</term>
<term>Wiley periodicals</term>
<term>Wistar rats</term>
<term>Xative solution</term>
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<div type="abstract">The aim of the present research was to analyze ultrastructural and immunohistochemical aspects of the alveolar repair after the extraction of molars of alendronate (ALN)‐treated rats. Wistar rats received 2.5mg/kg body wt/day of ALN during 14 days previously and 7, 14 and 21 days after the extraction of the second mandibular molar. Specimens were fixed in 2% glutaraldehyde + 2.5% formaldehyde under microwave irradiation, decalcified in 4.13% EDTA and paraffin embedded for TRAP histochemistry and immunohistochemistry for OPN, BSP and endoglin, or embedded in Spurr epoxy resin for TEM analysis. Additional specimens had their soft tissues removed and were processed for scanning electron microscopy. The ALN group presented latent TRAP‐positive osteoclasts and nonresorbed alveolar crests with bacterial infection. Mild bone necrosis signs were observed at all time points studied. Ultrastructurally, empty osteocyte lacunae were observed and bone trabeculae surface presented hyalinized aspect. A significant delay in alveolar repair occurred, as well as decreased angiogenesis. ALN treatment provoked mild signs of bone necrosis, despite the high dose employed. The present findings add new information about the ultrastructural aspect of the early repair of rats under ALN treatment and highlight for giving attention when oral surgeries are performed in patients using this drug. Microsc. Res. Tech. 76:633–640, 2013. © 2013 Wiley Periodicals, Inc.</div>
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