Osteoblasts behavior on chemically treated commercially pure titanium surfaces
Identifieur interne : 001E75 ( Main/Exploration ); précédent : 001E74; suivant : 001E76Osteoblasts behavior on chemically treated commercially pure titanium surfaces
Auteurs : D. P. Oliveira [Brésil] ; A. Palmieri [Italie] ; F. Carinci [Italie] ; C. Bolfarini [Brésil]Source :
- Journal of Biomedical Materials Research Part A [ 1549-3296 ] ; 2014-06.
Abstract
Surface modifications of commercially pure titanium (Cp‐Ti), a material widely used to produce dental implants, can induce specific responses on osteoblastic cells after implantation. This work aims to investigate the influence of chemically modified surfaces of Cp‐Ti by acid etching or acid etching plus alkaline treatment on the gene expression of human osteoblastic (Hob) cells. Roughness and contact angle measurements were carried out to evaluate the surface properties of the samples. The surface morphology was investigated with scanning electron microscopy. Chemical composition was analyzed by energy dispersive X‐ray spectroscopy (EDS). The expression levels of some bone‐related genes (ALPL, COL1A1, COL3A1, SPP1, RUNX2, and SPARC) were analyzed using real time Reverse Transcription‐Polymerase Chain Reaction (real time RT‐PCR). The results showed that all the chemical modifications studied in this work influenced the surface morphology, wettability, roughness and induced an osteoconductive behavior. The samples that were acid etched and alkaline treated showed a more pronounced effect. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1816–1822, 2014.
Url:
DOI: 10.1002/jbm.a.34855
Affiliations:
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P." last="Oliveira">D. P. Oliveira</name><affiliation wicri:level="1"><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Materials Engineering, Federal University of São Carlos, São Carlos</wicri:regionArea><wicri:noRegion>São Carlos</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Brésil</country></affiliation></author><author><name sortKey="Palmieri, A" sort="Palmieri, A" uniqKey="Palmieri A" first="A." last="Palmieri">A. Palmieri</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Maxillofacial Surgery, University of Ferrara, Ferrara</wicri:regionArea><wicri:noRegion>Ferrara</wicri:noRegion></affiliation></author><author><name sortKey="Carinci, F" sort="Carinci, F" uniqKey="Carinci F" first="F." last="Carinci">F. 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Bolfarini</name><affiliation wicri:level="1"><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Materials Engineering, Federal University of São Carlos, São Carlos</wicri:regionArea><wicri:noRegion>São Carlos</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Biomedical Materials Research Part A</title><title level="j" type="alt">JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A</title><idno type="ISSN">1549-3296</idno><idno type="eISSN">1552-4965</idno><imprint><biblScope unit="vol">102</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1816">1816</biblScope><biblScope unit="page" to="1822">1822</biblScope><biblScope unit="page-count">7</biblScope><date type="published" when="2014-06">2014-06</date></imprint><idno type="ISSN">1549-3296</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1549-3296</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Surface modifications of commercially pure titanium (Cp‐Ti), a material widely used to produce dental implants, can induce specific responses on osteoblastic cells after implantation. This work aims to investigate the influence of chemically modified surfaces of Cp‐Ti by acid etching or acid etching plus alkaline treatment on the gene expression of human osteoblastic (Hob) cells. Roughness and contact angle measurements were carried out to evaluate the surface properties of the samples. The surface morphology was investigated with scanning electron microscopy. Chemical composition was analyzed by energy dispersive X‐ray spectroscopy (EDS). The expression levels of some bone‐related genes (ALPL, COL1A1, COL3A1, SPP1, RUNX2, and SPARC) were analyzed using real time Reverse Transcription‐Polymerase Chain Reaction (real time RT‐PCR). The results showed that all the chemical modifications studied in this work influenced the surface morphology, wettability, roughness and induced an osteoconductive behavior. The samples that were acid etched and alkaline treated showed a more pronounced effect. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1816–1822, 2014.</div></front></TEI><affiliations><list><country><li>Brésil</li><li>Italie</li></country></list><tree><country name="Brésil"><noRegion><name sortKey="Oliveira, D P" sort="Oliveira, D P" uniqKey="Oliveira D" first="D. P." last="Oliveira">D. P. Oliveira</name></noRegion><name sortKey="Bolfarini, C" sort="Bolfarini, C" uniqKey="Bolfarini C" first="C." last="Bolfarini">C. Bolfarini</name><name sortKey="Oliveira, D P" sort="Oliveira, D P" uniqKey="Oliveira D" first="D. P." last="Oliveira">D. P. Oliveira</name></country><country name="Italie"><noRegion><name sortKey="Palmieri, A" sort="Palmieri, A" uniqKey="Palmieri A" first="A." last="Palmieri">A. Palmieri</name></noRegion><name sortKey="Carinci, F" sort="Carinci, F" uniqKey="Carinci F" first="F." last="Carinci">F. Carinci</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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