Periodontal pathogen-related stimulation indicates unique phenotype of primary cultured human fibroblasts from gingiva and periodontal ligament: Implications for oral health disease
Identifieur interne : 009D88 ( Main/Curation ); précédent : 009D87; suivant : 009D89Periodontal pathogen-related stimulation indicates unique phenotype of primary cultured human fibroblasts from gingiva and periodontal ligament: Implications for oral health disease
Auteurs : Sreenivas Koka ; Richard A. ReinhardtSource :
- The Journal of Prosthetic Dentistry [ 0022-3913 ] ; 1997.
Descripteurs français
- Wicri :
- topic : Médiateur.
English descriptors
- KwdEn :
- Active disease, Adult periodontitis, Alveolar bone loss, Assay, Bacterial virulence factors, Bone loss, Bone resorption, Cell population, Connective tissue, Culture supernatants, Cytokine, Dent, Dental implants, Dentistry, Edentulous patients, Extracellular matrix, Fibroblast, Fibroblast heterogeneity, Fibroblast subpopulations, Gentamicin sulfate, Gingival, Gingival fibroblasts, Gingivalis, Higher levels, Human gingival, Human gingival fibroblasts, Identical conditions, Implant, Independent variables, Inflammatory, Inflammatory mediator production, Inflammatory mediators, Interactive effects, Koka, Ligament, Ligament cells, Marginal bone height, Mediator, Mediator secretion, Mononuclear cells, Number2 koka, Oral maxillofac implants, Osseointegrated, Pdlf, Periodont, Periodontal, Periodontal disease, Periodontal ligament, Periodontal ligament cells, Periodontal ligament fibroblasts, Periodontal tissues, Periodontitis, Pge2, Pge2 levels, Pge2 production, Pge2 secretion, Predominant cell type, Prostaglandin, Prosthet dent, Prosthetic dentistry, Refractory periodontitis, Relevant group, Resorption, Tukey post, Unstimulated cells, Virulence factor.
- Teeft :
- Active disease, Adult periodontitis, Alveolar bone loss, Assay, Bacterial virulence factors, Bone loss, Bone resorption, Cell population, Connective tissue, Culture supernatants, Cytokine, Dent, Dental implants, Dentistry, Edentulous patients, Extracellular matrix, Fibroblast, Fibroblast heterogeneity, Fibroblast subpopulations, Gentamicin sulfate, Gingival, Gingival fibroblasts, Gingivalis, Higher levels, Human gingival, Human gingival fibroblasts, Identical conditions, Implant, Independent variables, Inflammatory, Inflammatory mediator production, Inflammatory mediators, Interactive effects, Koka, Ligament, Ligament cells, Marginal bone height, Mediator, Mediator secretion, Mononuclear cells, Number2 koka, Oral maxillofac implants, Osseointegrated, Pdlf, Periodont, Periodontal, Periodontal disease, Periodontal ligament, Periodontal ligament cells, Periodontal ligament fibroblasts, Periodontal tissues, Periodontitis, Pge2, Pge2 levels, Pge2 production, Pge2 secretion, Predominant cell type, Prostaglandin, Prosthet dent, Prosthetic dentistry, Refractory periodontitis, Relevant group, Resorption, Tukey post, Unstimulated cells, Virulence factor.
Abstract
Abstract: Statement of Problem. The fibroblast is considered an important cellular component in periodontitis because it is the predominant cell type in periodontal connective tissue. Purpose. The purpose of this study was to test whether gingival fibroblasts (GF) and periodontal ligament fibroblasts (PDLF) are heterogeneous in their production of inflammatory mediators associated with bone resorption in response to lipopolysaccharides from the gram-negative bacterium Porphyromonas gingivalis . To test this hypothesis, we (1) compared interleukin-6 production by cultured human PDLF and GF isolated from the same individual when exposed to P. gingivalis lipopolysaccharide; and (2) compared prostaglandin-E 2 production by cultured human PDLF and GF isolated from the same individual when exposed to P. gingivalis lipopolysaccharide. Material and Methods. Human periodontal ligament and gingival fibroblasts were cultured from biopsies and exposed to P. gingivalis lipopolysaccharide. Levels of interleukin-6 and prostaglandin-E 2 secreted into culture supernatant were measured by enzyme-linked immunosorbent assay and the data analyzed. Gingival fibroblasts secreted similar interleukin-6 and elevated prostaglandin-E 2 levels compared with unstimulated cells. Periodontal ligament fibroblasts secreted elevated levels of both mediators compared with unstimulated cells. Secreted levels of interleukin-6 and prostaglandin-E 2 by primary gingival and periodontal ligament fibroblast cultures appear to differ when stimulated with a periodontal pathogen-related virulence factor. Conclusion. The fibroblast of the periodontal ligament and the fibroblast of the gingiva may represent different phenotypes that play unique roles in tissue responses to implants. (J Prosthet Dent 1997:77:191-6.)
Url:
DOI: 10.1016/S0022-3913(97)70234-8
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Reinhardt</name><affiliation><wicri:noCountry code="subField">Neb</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j">The Journal of Prosthetic Dentistry</title><title level="j" type="abbrev">YMPR</title><idno type="ISSN">0022-3913</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1997">1997</date><biblScope unit="volume">77</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="191">191</biblScope><biblScope unit="page" to="196">196</biblScope></imprint><idno type="ISSN">0022-3913</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-3913</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Active disease</term><term>Adult periodontitis</term><term>Alveolar bone loss</term><term>Assay</term><term>Bacterial virulence factors</term><term>Bone loss</term><term>Bone resorption</term><term>Cell population</term><term>Connective tissue</term><term>Culture supernatants</term><term>Cytokine</term><term>Dent</term><term>Dental implants</term><term>Dentistry</term><term>Edentulous patients</term><term>Extracellular matrix</term><term>Fibroblast</term><term>Fibroblast heterogeneity</term><term>Fibroblast subpopulations</term><term>Gentamicin sulfate</term><term>Gingival</term><term>Gingival fibroblasts</term><term>Gingivalis</term><term>Higher levels</term><term>Human gingival</term><term>Human gingival fibroblasts</term><term>Identical conditions</term><term>Implant</term><term>Independent variables</term><term>Inflammatory</term><term>Inflammatory mediator production</term><term>Inflammatory mediators</term><term>Interactive effects</term><term>Koka</term><term>Ligament</term><term>Ligament cells</term><term>Marginal bone height</term><term>Mediator</term><term>Mediator secretion</term><term>Mononuclear cells</term><term>Number2 koka</term><term>Oral maxillofac implants</term><term>Osseointegrated</term><term>Pdlf</term><term>Periodont</term><term>Periodontal</term><term>Periodontal disease</term><term>Periodontal ligament</term><term>Periodontal ligament cells</term><term>Periodontal ligament fibroblasts</term><term>Periodontal tissues</term><term>Periodontitis</term><term>Pge2</term><term>Pge2 levels</term><term>Pge2 production</term><term>Pge2 secretion</term><term>Predominant cell type</term><term>Prostaglandin</term><term>Prosthet dent</term><term>Prosthetic dentistry</term><term>Refractory periodontitis</term><term>Relevant group</term><term>Resorption</term><term>Tukey post</term><term>Unstimulated cells</term><term>Virulence factor</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Active disease</term><term>Adult periodontitis</term><term>Alveolar bone loss</term><term>Assay</term><term>Bacterial virulence factors</term><term>Bone loss</term><term>Bone resorption</term><term>Cell population</term><term>Connective tissue</term><term>Culture supernatants</term><term>Cytokine</term><term>Dent</term><term>Dental implants</term><term>Dentistry</term><term>Edentulous patients</term><term>Extracellular matrix</term><term>Fibroblast</term><term>Fibroblast heterogeneity</term><term>Fibroblast subpopulations</term><term>Gentamicin sulfate</term><term>Gingival</term><term>Gingival fibroblasts</term><term>Gingivalis</term><term>Higher levels</term><term>Human gingival</term><term>Human gingival fibroblasts</term><term>Identical conditions</term><term>Implant</term><term>Independent variables</term><term>Inflammatory</term><term>Inflammatory mediator production</term><term>Inflammatory mediators</term><term>Interactive effects</term><term>Koka</term><term>Ligament</term><term>Ligament cells</term><term>Marginal bone height</term><term>Mediator</term><term>Mediator secretion</term><term>Mononuclear cells</term><term>Number2 koka</term><term>Oral maxillofac implants</term><term>Osseointegrated</term><term>Pdlf</term><term>Periodont</term><term>Periodontal</term><term>Periodontal disease</term><term>Periodontal ligament</term><term>Periodontal ligament cells</term><term>Periodontal ligament fibroblasts</term><term>Periodontal tissues</term><term>Periodontitis</term><term>Pge2</term><term>Pge2 levels</term><term>Pge2 production</term><term>Pge2 secretion</term><term>Predominant cell type</term><term>Prostaglandin</term><term>Prosthet dent</term><term>Prosthetic dentistry</term><term>Refractory periodontitis</term><term>Relevant group</term><term>Resorption</term><term>Tukey post</term><term>Unstimulated cells</term><term>Virulence factor</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Médiateur</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Statement of Problem. The fibroblast is considered an important cellular component in periodontitis because it is the predominant cell type in periodontal connective tissue. Purpose. The purpose of this study was to test whether gingival fibroblasts (GF) and periodontal ligament fibroblasts (PDLF) are heterogeneous in their production of inflammatory mediators associated with bone resorption in response to lipopolysaccharides from the gram-negative bacterium Porphyromonas gingivalis . To test this hypothesis, we (1) compared interleukin-6 production by cultured human PDLF and GF isolated from the same individual when exposed to P. gingivalis lipopolysaccharide; and (2) compared prostaglandin-E 2 production by cultured human PDLF and GF isolated from the same individual when exposed to P. gingivalis lipopolysaccharide. Material and Methods. Human periodontal ligament and gingival fibroblasts were cultured from biopsies and exposed to P. gingivalis lipopolysaccharide. Levels of interleukin-6 and prostaglandin-E 2 secreted into culture supernatant were measured by enzyme-linked immunosorbent assay and the data analyzed. Gingival fibroblasts secreted similar interleukin-6 and elevated prostaglandin-E 2 levels compared with unstimulated cells. Periodontal ligament fibroblasts secreted elevated levels of both mediators compared with unstimulated cells. Secreted levels of interleukin-6 and prostaglandin-E 2 by primary gingival and periodontal ligament fibroblast cultures appear to differ when stimulated with a periodontal pathogen-related virulence factor. Conclusion. The fibroblast of the periodontal ligament and the fibroblast of the gingiva may represent different phenotypes that play unique roles in tissue responses to implants. (J Prosthet Dent 1997:77:191-6.)</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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