An ectopic study of apatite-coated silk fibroin scaffolds seeded with AdBMP-2 modified canine bMSCs
Identifieur interne : 004308 ( Main/Curation ); précédent : 004307; suivant : 004309An ectopic study of apatite-coated silk fibroin scaffolds seeded with AdBMP-2 modified canine bMSCs
Auteurs : Kaige Lü [République populaire de Chine] ; Ling Xu [République populaire de Chine] ; Lunguo Xia [République populaire de Chine] ; Yilin Zhang [République populaire de Chine] ; Xiuli Zhang [République populaire de Chine] ; David L. Kaplan [États-Unis] ; Xinquan Jiang [République populaire de Chine] ; Fuqiang Zhang [République populaire de Chine]Source :
- Journal of Biomaterials Science. Polymer Edition [ 0920-5063 ] ; 2011.
Abstract
The present study was undertaken to evaluate ectopic new bone formation effects of apatite-coated silk fibroin scaffolds (mSS) seeded with adenovirus mediated bone morphogenic protein-2 gene (AdBMP-2) transduced canine bone marrow stromal cells (bMSCs) in nude mice. In this study, bMSCs derived from canine were cultured and transduced with AdBMP-2, adenovirus mediated enhanced green fluorescent protein gene (AdEGFP) in vitro. Osteogenic differentiation of bMSCs was determined by alkaline phosphatase (ALP) activity analysis, and the transcript levels for BMP-2, osteopontin (OPN), osteocalcin (OCN) and bone sialoprotein (BSP) genes via real-time quantitative PCR (RT-qPCR) analysis. The ectopic bone formation effects of mSS seeded with AdBMP-2 modified bMSCs were evaluated through histological and histomorphological analysis 4, 8 and 12 weeks post-operation in nude mice. ALP activity was statistically increased in AdBMP-2 group, when compared with control groups. The mRNA expression of BMP-2, OPN, OCN and BSP also statistically up-regulated 6, 9 days after AdBMP-2 transduction. Significantly higher bone volume was achieved in AdBMP-2-transduced bMSCs/mSS constructs than that of AdEGFP-transduced bMSCs/mSS or bMSCs/mSS groups at 4, 8 and 12 weeks (
Url:
DOI: 10.1163/092050610X552861
PubMed: 21294971
PubMed Central: 3406182
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002754
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :002754
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :002249
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :003429
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :003429
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :003429
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :004335
Links to Exploration step
PMC:3406182Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An ectopic study of apatite-coated silk fibroin scaffolds seeded with AdBMP-2 modified canine bMSCs</title>
<author><name sortKey="Lu, Kaige" sort="Lu, Kaige" uniqKey="Lu K" first="Kaige" last="Lü">Kaige Lü</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xu, Ling" sort="Xu, Ling" uniqKey="Xu L" first="Ling" last="Xu">Ling Xu</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xia, Lunguo" sort="Xia, Lunguo" uniqKey="Xia L" first="Lunguo" last="Xia">Lunguo Xia</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Department of Oral and Maxillofacial Surgery, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Oral and Maxillofacial Surgery, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011</wicri:regionArea>
<wicri:noRegion>Shanghai 200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Yilin" sort="Zhang, Yilin" uniqKey="Zhang Y" first="Yilin" last="Zhang">Yilin Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Xiuli" sort="Zhang, Xiuli" uniqKey="Zhang X" first="Xiuli" last="Zhang">Xiuli Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="A3">Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011</wicri:regionArea>
<wicri:noRegion>Shanghai 200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kaplan, David L" sort="Kaplan, David L" uniqKey="Kaplan D" first="David L." last="Kaplan">David L. Kaplan</name>
<affiliation wicri:level="1"><nlm:aff id="A4">Department of Biomedical Engineering, Tufts University, Massachusetts 02155, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biomedical Engineering, Tufts University, Massachusetts 02155</wicri:regionArea>
<wicri:noRegion>Massachusetts 02155</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jiang, Xinquan" sort="Jiang, Xinquan" uniqKey="Jiang X" first="Xinquan" last="Jiang">Xinquan Jiang</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A3">Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011</wicri:regionArea>
<wicri:noRegion>Shanghai 200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Fuqiang" sort="Zhang, Fuqiang" uniqKey="Zhang F" first="Fuqiang" last="Zhang">Fuqiang Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">21294971</idno>
<idno type="pmc">3406182</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3406182</idno>
<idno type="RBID">PMC:3406182</idno>
<idno type="doi">10.1163/092050610X552861</idno>
<date when="2011">2011</date>
<idno type="wicri:Area/Pmc/Corpus">002754</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002754</idno>
<idno type="wicri:Area/Pmc/Curation">002754</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002754</idno>
<idno type="wicri:Area/Pmc/Checkpoint">002249</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002249</idno>
<idno type="wicri:Area/Ncbi/Merge">003429</idno>
<idno type="wicri:Area/Ncbi/Curation">003429</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">003429</idno>
<idno type="wicri:doubleKey">0920-5063:2011:Lu K:an:ectopic:study</idno>
<idno type="wicri:Area/Main/Merge">004335</idno>
<idno type="wicri:Area/Main/Curation">004308</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">An ectopic study of apatite-coated silk fibroin scaffolds seeded with AdBMP-2 modified canine bMSCs</title>
<author><name sortKey="Lu, Kaige" sort="Lu, Kaige" uniqKey="Lu K" first="Kaige" last="Lü">Kaige Lü</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xu, Ling" sort="Xu, Ling" uniqKey="Xu L" first="Ling" last="Xu">Ling Xu</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xia, Lunguo" sort="Xia, Lunguo" uniqKey="Xia L" first="Lunguo" last="Xia">Lunguo Xia</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Department of Oral and Maxillofacial Surgery, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Oral and Maxillofacial Surgery, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011</wicri:regionArea>
<wicri:noRegion>Shanghai 200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Yilin" sort="Zhang, Yilin" uniqKey="Zhang Y" first="Yilin" last="Zhang">Yilin Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Xiuli" sort="Zhang, Xiuli" uniqKey="Zhang X" first="Xiuli" last="Zhang">Xiuli Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="A3">Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011</wicri:regionArea>
<wicri:noRegion>Shanghai 200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kaplan, David L" sort="Kaplan, David L" uniqKey="Kaplan D" first="David L." last="Kaplan">David L. Kaplan</name>
<affiliation wicri:level="1"><nlm:aff id="A4">Department of Biomedical Engineering, Tufts University, Massachusetts 02155, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biomedical Engineering, Tufts University, Massachusetts 02155</wicri:regionArea>
<wicri:noRegion>Massachusetts 02155</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jiang, Xinquan" sort="Jiang, Xinquan" uniqKey="Jiang X" first="Xinquan" last="Jiang">Xinquan Jiang</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A3">Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011</wicri:regionArea>
<wicri:noRegion>Shanghai 200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Fuqiang" sort="Zhang, Fuqiang" uniqKey="Zhang F" first="Fuqiang" last="Zhang">Fuqiang Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Biomaterials Science. Polymer Edition</title>
<idno type="ISSN">0920-5063</idno>
<idno type="eISSN">1568-5624</idno>
<imprint><date when="2011">2011</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">The present study was undertaken to evaluate ectopic new bone formation effects of apatite-coated silk fibroin scaffolds (mSS) seeded with adenovirus mediated bone morphogenic protein-2 gene (AdBMP-2) transduced canine bone marrow stromal cells (bMSCs) in nude mice. In this study, bMSCs derived from canine were cultured and transduced with AdBMP-2, adenovirus mediated enhanced green fluorescent protein gene (AdEGFP) in vitro. Osteogenic differentiation of bMSCs was determined by alkaline phosphatase (ALP) activity analysis, and the transcript levels for BMP-2, osteopontin (OPN), osteocalcin (OCN) and bone sialoprotein (BSP) genes via real-time quantitative PCR (RT-qPCR) analysis. The ectopic bone formation effects of mSS seeded with AdBMP-2 modified bMSCs were evaluated through histological and histomorphological analysis 4, 8 and 12 weeks post-operation in nude mice. ALP activity was statistically increased in AdBMP-2 group, when compared with control groups. The mRNA expression of BMP-2, OPN, OCN and BSP also statistically up-regulated 6, 9 days after AdBMP-2 transduction. Significantly higher bone volume was achieved in AdBMP-2-transduced bMSCs/mSS constructs than that of AdEGFP-transduced bMSCs/mSS or bMSCs/mSS groups at 4, 8 and 12 weeks (<italic>P</italic>
<0.01). These results demonstrated that mSS seeded with AdBMP-2-transduced canine bMSCs can promote ectopic new bone formation and maturation in nude mice suggesting the potential of this silk scaffold based tissue engineered bone for further bone regeneration studies in canine models.</p>
</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/EdenteV2/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004308 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 004308 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Santé |area= EdenteV2 |flux= Main |étape= Curation |type= RBID |clé= PMC:3406182 |texte= An ectopic study of apatite-coated silk fibroin scaffolds seeded with AdBMP-2 modified canine bMSCs }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:21294971" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \ | NlmPubMed2Wicri -a EdenteV2
This area was generated with Dilib version V0.6.32. |